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Reviewed, UniProtKB/Swiss-Prot P32721 (ALSA_ECOLI)

Last modified June 16, 2009. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    D-allose import ATP-binding protein alsA
    EC=3.6.3.17
Gene names
Name: alsA
Synonyms: yjcW
Ordered Locus Names: b4087, JW4048
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length510 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Part of the ABC transporter complex alsBAC involved in D-allose import. Probably responsible for energy coupling to the transport system. Ref.5

Catalytic activity

ATP + H2O + monosaccharide(Out) = ADP + phosphate + monosaccharide(In).

Subunit structure

The complex is composed of two ATP-binding proteins (alsA), two transmembrane proteins (alsC) and a solute-binding protein (alsB) Probable.

Subcellular location

Cell inner membrane; Peripheral membrane protein By similarity.

Sequence similarities

Belongs to the ABC transporter family. D-allose importer (TC 3.A.1.2.6) subfamily. [View classification]

Contains 2 ABC transporter domains.

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Ontologies

Keywords
   Biological processSugar transport
Transport
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainRepeat
   LigandATP-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcarbohydrate transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

monosaccharide-transporting ATPase activity

Inferred from electronic annotation. Source: EC

protein binding

Inferred from physical interaction. Source: IntAct

sugar:hydrogen symporter activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

groLP0A6F51EBI-562166,EBI-543750

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 510510D-allose import ATP-binding protein alsA
PRO_0000091926

Regions

Domain6 – 245240ABC transporter 1
Domain260 – 509250ABC transporter 2
Nucleotide binding38 – 458ATP Potential

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Sequences

Sequence LengthMass (Da)Tools
P32721-1 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 733FA8ABD3618EE1

FASTA51056,745
        10         20         30         40         50         60 
MATPYISMAG IGKSFGPVHA LKSVNLTVYP GEIHALLGEN GAGKSTLMKV LSGIHEPTKG 

        70         80         90        100        110        120 
TITINNISYN KLDHKLAAQL GIGIIYQELS VIDELTVLEN LYIGRHLTKK ICGVNIIDWR 

       130        140        150        160        170        180 
EMRVRAAMML LRVGLKVDLD EKVANLSISH KQMLEIAKTL MLDAKVIIMD EPTSSLTNKE 

       190        200        210        220        230        240 
VDYLFLIMNQ LRKEGTAIVY ISHKLAEIRR ICDRYTVMKD GSSVCSGIVS DVSNDDIVRL 

       250        260        270        280        290        300 
MVGRELQNRF NAMKENVSNL AHETVFEVRN VTSRDRKKVR DISFSVCRGE ILGFAGLVGS 

       310        320        330        340        350        360 
GRTELMNCLF GVDKRAGGEI RLNGKDISPR SPLDAVKKGM AYITESRRDN GFFPNFSIAQ 

       370        380        390        400        410        420 
NMAISRSLKD GGYKGAMGLF HEVDEQRTAE NQRELLALKC HSVNQNITEL SGGNQQKVLI 

       430        440        450        460        470        480 
SKWLCCCPEV IIFDEPTRGI DVGAKAEIYK VMRQLADDGK VILMVSSELP EIITVCDRIA 

       490        500        510 
VFCEGRLTQI LTNRDDMSEE EIMAWALPQE

« Hide

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References

« Hide 'large scale' references
[1]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 9-510.
Strain: K12 / MG1655 / ATCC 47076.
[5]"The D-allose operon of Escherichia coli K-12."
Kim C., Song S., Park C.
J. Bacteriol. 179:7631-7637(1997) [PubMed: 9401019] [Abstract]
Cited for: FUNCTION.

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Cross-references

Sequence databases

U14003 Genomic DNA. Translation: AAA96986.1.
U00096 Genomic DNA. Translation: AAC77048.1.
AP009048 Genomic DNA. Translation: BAE78090.1.
U00006 Genomic DNA. Translation: AAC43181.1.
PIRS56315.
RefSeqAP_004589.1.
NP_418511.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:9093N.
IntActP32721. 5 interactions.

Protein family/group databases

TCDB3.A.1.2.6. ATP-binding cassette (ABC) superfamily.

Genome annotation databases

GeneID948593.
GenomeReviewsGene locus JW4048 in contig AP009048_GR.
Gene locus b4087 in contig U00096_GR.
KEGGecj:JW4048.
eco:b4087.

Organism-specific databases

EchoBASEEB1902.
EcoGeneEG11959. alsA.
CMRSearch...

Phylogenomic databases

HOGENOMP32721.
OMAP32721. HSINQNI.

Enzyme and pathway databases

BioCycEcoCyc:YJCW-MON.

Family and domain databases

InterProIPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR003593. ATPase_AAA+_core.
[Graphical view]
PfamPF00005. ABC_tran. 2 hits.
[Graphical view]
ProDomPD000006. ABC_transporter. 2 hits.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
PROSITEPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameALSA_ECOLI
AccessionPrimary (citable) accession number: P32721
Secondary accession number(s): Q2M6L6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1995
Last modified: June 16, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents