P32716 (MDTN_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Multidrug resistance protein MdtN

Gene names

Name:	mdtN
Synonyms:	yjcR
Ordered Locus Names:	b4082, JW4043

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Could be involved in resistance to puromycin, acriflavine and tetraphenylarsonium chloride. Ref.4
Subunit structure	Could be part of a tripartite efflux system composed of MdtN, MdtO and MdtP.
Subcellular location	Cell inner membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family. [View classification]

Ontologies

Keywords
Biological process	Antibiotic resistance Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Gram-negative-bacterium-type cell wall Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	drug transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Multidrug resistance protein MdtN		PRO_0000201883
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 33	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	34 – 343	310	Periplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P32716 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: C599B8707C475FFE	FASTA	343	36,908
	10 20 30 40 50 60 MESTPKKAPR SKFPALLVVA LALVALVFVI WRVDSAPSTN DAYASADTID VVPEVSGRIV 70 80 90 100 110 120 ELAVTDNQAV KQGDLLFRID PRPYEANLAK AEASLAALDK QIMLTQRSVD AQQFGADSVN 130 140 150 160 170 180 ATVEKARAAA KQATDTLRRT EPLLKEGFVS AEDVDRARTA QRAAEADLNA VLLQAQSAAS 190 200 210 220 230 240 AVSGVDALVA QRAAVEADIA LTKLHLEMAT VRAPFDGRVI SLKTSVGQFA SAMRPIFTLI 250 260 270 280 290 300 DTRHWYVIAN FRETDLKNIR SGTPATIRLM SDSGKTFEGK VDSIGYGVLP DDGGLVLGGL 310 320 330 340 PKVSRSINWV RVAQRFPVKI MVDKPDPEMF RIGASAVANL EPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Antibiotic susceptibility profiles of Escherichia coli strains lacking multidrug efflux pump genes." Sulavik M.C., Houseweart C., Cramer C., Jiwani N., Murgolo N., Greene J., DiDomenico B., Shaw K.J., Miller G.H., Hare R., Shimer G. Antimicrob. Agents Chemother. 45:1126-1136(2001) [PubMed: 11257026] [Abstract] Cited for: PUTATIVE FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00006 Genomic DNA. Translation: AAC43176.1. U00096 Genomic DNA. Translation: AAD13465.1. AP009048 Genomic DNA. Translation: BAE78084.1.
PIR	A65217.
RefSeq	NP_418506.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P32716.
SMR	P32716. Positions 47-342.
ModBase	Search...
Protein-protein interaction databases
IntAct	P32716. 3 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003131; EBESCP00000003131; EBESCG00000002567. EBESCT00000016625; EBESCP00000015916; EBESCG00000015684.
GeneID	948598.
GenomeReviews	Gene locus JW4043 in contig AP009048_GR. Gene locus b4082 in contig U00096_GR.
KEGG	ecj:JW4043. eco:b4082.
PATRIC	32123717. VBIEscCol129921_4207.
Organism-specific databases
EchoBASE	EB1897.
EcoGene	EG11954. mdtN.
Phylogenomic databases
eggNOG	COG1566.
GeneTree	EBGT00050000009283.
HOGENOM	HBG624965.
OMA	HLEMTTV.
PhylomeDB	P32716.
ProtClustDB	PRK10476.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11954-MONOMER.
Gene expression databases
Genevestigator	P32716.
Family and domain databases
InterPro	IPR005694. Emr. IPR006143. RND_pump_MFP. [Graphical view]
KO	K15549.
Pfam	PF00529. HlyD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00998. 8a0101. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDTN_ECOLI

Accession

Primary (citable) accession number: P32716
Secondary accession number(s): Q2M6M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	January 25, 2012
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program