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Protein

Multidrug resistance protein MdtO

Gene

mdtO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Could be involved in resistance to puromycin, acriflavine and tetraphenylarsonium chloride.

GO - Molecular functioni

  • sulfathiazole transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • response to antibiotic Source: UniProtKB-KW
  • sulfathiazole transmembrane transport Source: GOC
  • sulfathiazole transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YJCQ-MONOMER.
ECOL316407:JW4042-MONOMER.

Protein family/group databases

TCDBi2.A.85.6.1. the aromatic acid exporter (arae) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein MdtO
Gene namesi
Name:mdtO
Synonyms:yjcQ
Ordered Locus Names:b4081, JW4042
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11953. mdtO.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei43 – 6321HelicalSequence analysisAdd
BLAST
Transmembranei75 – 9521HelicalSequence analysisAdd
BLAST
Transmembranei100 – 12021HelicalSequence analysisAdd
BLAST
Transmembranei125 – 14521HelicalSequence analysisAdd
BLAST
Transmembranei158 – 17821HelicalSequence analysisAdd
BLAST
Transmembranei402 – 42221HelicalSequence analysisAdd
BLAST
Transmembranei426 – 44621HelicalSequence analysisAdd
BLAST
Transmembranei457 – 47721HelicalSequence analysisAdd
BLAST
Transmembranei483 – 50321HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 683683Multidrug resistance protein MdtOPRO_0000210089Add
BLAST

Proteomic databases

PaxDbiP32715.
PRIDEiP32715.

Expressioni

Inductioni

Induced by LeuO, part of the mdtNO operon.1 Publication

Interactioni

Subunit structurei

Could be part of a tripartite efflux system composed of MdtN, MdtO and MdtP.

Binary interactionsi

WithEntry#Exp.IntActNotes
pheTP073953EBI-555775,EBI-555713
yeaJP762372EBI-555775,EBI-555763

Protein-protein interaction databases

BioGridi4262952. 6 interactions.
DIPiDIP-12556N.
IntActiP32715. 3 interactions.
MINTiMINT-1312912.
STRINGi511145.b4081.

Structurei

3D structure databases

ProteinModelPortaliP32715.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MdtO family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108MI0. Bacteria.
COG1289. LUCA.
HOGENOMiHOG000126953.
KOiK15547.
OMAiQSDWRIT.
OrthoDBiEOG6WMHV2.

Family and domain databases

InterProiIPR006726. PHBA_efflux_AaeB/fusaric-R.
[Graphical view]
PfamiPF04632. FUSC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32715-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALNSLPLP VVRLLAFFHE ELSERRPGRV PQTVQLWVGC LLVILISMTF
60 70 80 90 100
EIPFVALSLA VLFYGIQSNA FYTKFVAILF VVATVLEIGS LFLIYKWSYG
110 120 130 140 150
EPLIRLIIAG PILMGCMFLM RTHRLGLVFF AVAIVAIYGQ TFPAMLDYPE
160 170 180 190 200
VVVRLTLWCI VVGLYPTLLM TLIGVLWFPS RAISQMHQAL NDRLDDAISH
210 220 230 240 250
LTDSLAPLPE TRIEREALAL QKLNVFCLAD DANWRTQNAW WQSCVATVTY
260 270 280 290 300
IYSTLNRYDP TSFADSQAII EFRQKLASEI NKLQHAVAEG QCWQSDWRIS
310 320 330 340 350
ESEAMAAREC NLENICQTLL QLGQMDPNTP PTPAAKPPSM AADAFTNPDY
360 370 380 390 400
MRYAVKTLLA CLICYTFYSG VDWEGIHTCM LTCVIVANPN VGSSYQKMVL
410 420 430 440 450
RFGGAFCGAI LALLFTLLVM PWLDNIVELL FVLAPIFLLG AWIATSSERS
460 470 480 490 500
SYIGTQMVVT FALATLENVF GPVYDLVEIR DRALGIIIGT VVSAVIYTFV
510 520 530 540 550
WPESEARTLP QKLAGTLGML SKVMRIPRQQ EVTALRTYLQ IRIGLHAAFN
560 570 580 590 600
ACEEMCQRVA LERQLDSEER ALLIERSQTV IRQGRDLLHA WDATWNSAQA
610 620 630 640 650
LDNALQPDRA GQFADALEKY AAGLATALSR SPQITLEETP ASQAILPTLL
660 670 680
KQEQHVCQLF ARLPDWTAPA LTPATEQAQG ATQ
Length:683
Mass (Da):76,151
Last modified:July 15, 1998 - v2
Checksum:iF9BE67EE4E01921C
GO

Sequence cautioni

The sequence AAC43175.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43175.1. Different initiation.
U00096 Genomic DNA. Translation: AAD13464.2.
AP009048 Genomic DNA. Translation: BAE78083.1.
PIRiH65216.
RefSeqiNP_418505.2. NC_000913.3.
WP_001275207.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAD13464; AAD13464; b4081.
BAE78083; BAE78083; BAE78083.
GeneIDi948582.
KEGGiecj:JW4042.
eco:b4081.
PATRICi32123715. VBIEscCol129921_4206.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43175.1. Different initiation.
U00096 Genomic DNA. Translation: AAD13464.2.
AP009048 Genomic DNA. Translation: BAE78083.1.
PIRiH65216.
RefSeqiNP_418505.2. NC_000913.3.
WP_001275207.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP32715.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262952. 6 interactions.
DIPiDIP-12556N.
IntActiP32715. 3 interactions.
MINTiMINT-1312912.
STRINGi511145.b4081.

Protein family/group databases

TCDBi2.A.85.6.1. the aromatic acid exporter (arae) family.

Proteomic databases

PaxDbiP32715.
PRIDEiP32715.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD13464; AAD13464; b4081.
BAE78083; BAE78083; BAE78083.
GeneIDi948582.
KEGGiecj:JW4042.
eco:b4081.
PATRICi32123715. VBIEscCol129921_4206.

Organism-specific databases

EchoBASEiEB1896.
EcoGeneiEG11953. mdtO.

Phylogenomic databases

eggNOGiENOG4108MI0. Bacteria.
COG1289. LUCA.
HOGENOMiHOG000126953.
KOiK15547.
OMAiQSDWRIT.
OrthoDBiEOG6WMHV2.

Enzyme and pathway databases

BioCyciEcoCyc:YJCQ-MONOMER.
ECOL316407:JW4042-MONOMER.

Miscellaneous databases

PROiP32715.

Family and domain databases

InterProiIPR006726. PHBA_efflux_AaeB/fusaric-R.
[Graphical view]
PfamiPF04632. FUSC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
    Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
    Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Antibiotic susceptibility profiles of Escherichia coli strains lacking multidrug efflux pump genes."
    Sulavik M.C., Houseweart C., Cramer C., Jiwani N., Murgolo N., Greene J., DiDomenico B., Shaw K.J., Miller G.H., Hare R., Shimer G.
    Antimicrob. Agents Chemother. 45:1126-1136(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PUTATIVE FUNCTION.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux."
    Shimada T., Yamamoto K., Ishihama A.
    J. Bacteriol. 191:4562-4571(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: OPERON STRUCTURE, INDUCTION.
    Strain: K12 / BW25113.

Entry informationi

Entry nameiMDTO_ECOLI
AccessioniPrimary (citable) accession number: P32715
Secondary accession number(s): Q2M6M3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: July 15, 1998
Last modified: January 20, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.