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Protein

Formate-dependent nitrite reductase complex subunit NrfF

Gene

nrfF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Not required for the biosynthesis of any of the c-type cytochromes. Possible subunit of a heme lyase.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44Heme (covalent)Sequence analysis1
Binding sitei47Heme (covalent)Sequence analysis1

GO - Molecular functioni

GO - Biological processi

  • cytochrome c-heme linkage via heme-L-cysteine Source: EcoCyc

Keywordsi

LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11949-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate-dependent nitrite reductase complex subunit NrfF
Gene namesi
Name:nrfF
Synonyms:yjcM
Ordered Locus Names:b4075, JW4036
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11949. nrfF.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Triple nrfE-nrfF-nrfG deletion mutants no longer attach heme 1 (covalently attached to "Cys-122", the CWSCK motif) of cytochrome c552 (AC P0ABK9).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000000661428 – 127Formate-dependent nitrite reductase complex subunit NrfFAdd BLAST100

Proteomic databases

PaxDbiP32711.
PRIDEiP32711.

Interactioni

Protein-protein interaction databases

BioGridi4262678. 9 interactors.
STRINGi316385.ECDH10B_4266.

Structurei

3D structure databases

ProteinModelPortaliP32711.
SMRiP32711.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CcmH/CycL/Ccl2/NrfF family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3088. LUCA.
HOGENOMiHOG000274670.
InParanoidiP32711.
KOiK04017.
PhylomeDBiP32711.

Family and domain databases

InterProiView protein in InterPro
IPR005616. CcmH/CycL/Ccl2/NrfF.
IPR017565. For-dep_Cytc_NO2Rdtase_NrfF.
PfamiView protein in Pfam
PF03918. CcmH. 1 hit.
TIGRFAMsiTIGR03147. cyt_nit_nrfF. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32711-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKGLLTLLL LFTCFAHAQV VDTWQFANPQ QQQQALNIAS QLRCPQCQNQ
60 70 80 90 100
NLLESNAPVA VSMRHQVYSM VAEGKNEVEI IGWMTERYGD FVRYNPPLTG
110 120
QTLVLWALPV VLLLLMALIL WRVRAKR
Length:127
Mass (Da):14,523
Last modified:October 1, 1993 - v1
Checksum:iAFE4404B9A398231
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72298 Genomic DNA. Translation: CAA51046.1.
U00006 Genomic DNA. Translation: AAC43169.1.
U00096 Genomic DNA. Translation: AAD13458.1.
AP009048 Genomic DNA. Translation: BAE78077.1.
PIRiF57987.
RefSeqiNP_418499.1. NC_000913.3.
WP_001032531.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAD13458; AAD13458; b4075.
BAE78077; BAE78077; BAE78077.
GeneIDi948578.
KEGGiecj:JW4036.
eco:b4075.
PATRICifig|1411691.4.peg.2629.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNRFF_ECOLI
AccessioniPrimary (citable) accession number: P32711
Secondary accession number(s): Q2M6M9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 5, 2017
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families