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Protein

Formate-dependent nitrite reductase complex subunit NrfF

Gene

nrfF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Not required for the biosynthesis of any of the c-type cytochromes. Possible subunit of a heme lyase.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441Heme (covalent)Sequence analysis
Binding sitei47 – 471Heme (covalent)Sequence analysis

GO - Molecular functioni

GO - Biological processi

  • cytochrome c-heme linkage via heme-L-cysteine Source: EcoCyc
Complete GO annotation...

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11949-MONOMER.
ECOL316407:JW4036-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate-dependent nitrite reductase complex subunit NrfF
Gene namesi
Name:nrfF
Synonyms:yjcM
Ordered Locus Names:b4075, JW4036
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11949. nrfF.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence analysisAdd
BLAST
Chaini28 – 127100Formate-dependent nitrite reductase complex subunit NrfFPRO_0000006614Add
BLAST

Proteomic databases

PaxDbiP32711.

Interactioni

Protein-protein interaction databases

BioGridi4262678. 9 interactions.
STRINGi511145.b4075.

Structurei

3D structure databases

ProteinModelPortaliP32711.
SMRiP32711. Positions 21-97.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CcmH/CycL/Ccl2/NrfF family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3088. LUCA.
HOGENOMiHOG000274670.
InParanoidiP32711.
KOiK04017.
OMAiLMILILW.
OrthoDBiEOG679TDV.
PhylomeDBiP32711.

Family and domain databases

InterProiIPR005616. CcmH/CycL/Ccl2/NrfF.
IPR017565. For-dep_Cytc_NO2Rdtase_NrfF.
[Graphical view]
PfamiPF03918. CcmH. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03147. cyt_nit_nrfF. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32711-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKGLLTLLL LFTCFAHAQV VDTWQFANPQ QQQQALNIAS QLRCPQCQNQ
60 70 80 90 100
NLLESNAPVA VSMRHQVYSM VAEGKNEVEI IGWMTERYGD FVRYNPPLTG
110 120
QTLVLWALPV VLLLLMALIL WRVRAKR
Length:127
Mass (Da):14,523
Last modified:October 1, 1993 - v1
Checksum:iAFE4404B9A398231
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72298 Genomic DNA. Translation: CAA51046.1.
U00006 Genomic DNA. Translation: AAC43169.1.
U00096 Genomic DNA. Translation: AAD13458.1.
AP009048 Genomic DNA. Translation: BAE78077.1.
PIRiF57987.
RefSeqiNP_418499.1. NC_000913.3.
WP_001032531.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAD13458; AAD13458; b4075.
BAE78077; BAE78077; BAE78077.
GeneIDi948578.
KEGGiecj:JW4036.
eco:b4075.
PATRICi32123699. VBIEscCol129921_4198.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72298 Genomic DNA. Translation: CAA51046.1.
U00006 Genomic DNA. Translation: AAC43169.1.
U00096 Genomic DNA. Translation: AAD13458.1.
AP009048 Genomic DNA. Translation: BAE78077.1.
PIRiF57987.
RefSeqiNP_418499.1. NC_000913.3.
WP_001032531.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP32711.
SMRiP32711. Positions 21-97.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262678. 9 interactions.
STRINGi511145.b4075.

Proteomic databases

PaxDbiP32711.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD13458; AAD13458; b4075.
BAE78077; BAE78077; BAE78077.
GeneIDi948578.
KEGGiecj:JW4036.
eco:b4075.
PATRICi32123699. VBIEscCol129921_4198.

Organism-specific databases

EchoBASEiEB1892.
EcoGeneiEG11949. nrfF.

Phylogenomic databases

eggNOGiCOG3088. LUCA.
HOGENOMiHOG000274670.
InParanoidiP32711.
KOiK04017.
OMAiLMILILW.
OrthoDBiEOG679TDV.
PhylomeDBiP32711.

Enzyme and pathway databases

BioCyciEcoCyc:EG11949-MONOMER.
ECOL316407:JW4036-MONOMER.

Miscellaneous databases

PROiP32711.

Family and domain databases

InterProiIPR005616. CcmH/CycL/Ccl2/NrfF.
IPR017565. For-dep_Cytc_NO2Rdtase_NrfF.
[Graphical view]
PfamiPF03918. CcmH. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03147. cyt_nit_nrfF. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A seven-gene operon essential for formate-dependent nitrite reduction to ammonia by enteric bacteria."
    Hussain H.A., Grove J., Griffiths L., Busby S., Cole J.
    Mol. Microbiol. 12:153-163(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
    Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
    Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The role of the genes nrf EFG and ccmFH in cytochrome c biosynthesis in Escherichia coli."
    Grovc J., Busby S., Cole J.
    Mol. Gen. Genet. 252:332-341(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE FUNCTION.
    Strain: K12.

Entry informationi

Entry nameiNRFF_ECOLI
AccessioniPrimary (citable) accession number: P32711
Secondary accession number(s): Q2M6M9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: January 20, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.