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Protein

Cytochrome c-type biogenesis protein NrfE

Gene

nrfE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

May be required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase.

GO - Molecular functioni

GO - Biological processi

  • cytochrome c-heme linkage via heme-L-cysteine Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Cytochrome c-type biogenesis

Enzyme and pathway databases

BioCyciEcoCyc:EG11948-MONOMER.
ECOL316407:JW4035-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c-type biogenesis protein NrfE
Gene namesi
Name:nrfE
Synonyms:yjcL
Ordered Locus Names:b4074, JW4035
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11948. nrfE.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei26 – 46HelicalSequence analysisAdd BLAST21
Transmembranei77 – 97HelicalSequence analysisAdd BLAST21
Transmembranei105 – 125HelicalSequence analysisAdd BLAST21
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Transmembranei193 – 213HelicalSequence analysisAdd BLAST21
Transmembranei228 – 247HelicalSequence analysisAdd BLAST20
Transmembranei256 – 276HelicalSequence analysisAdd BLAST21
Transmembranei294 – 314HelicalSequence analysisAdd BLAST21
Transmembranei333 – 353HelicalSequence analysisAdd BLAST21
Transmembranei376 – 396HelicalSequence analysisAdd BLAST21
Transmembranei399 – 419HelicalSequence analysisAdd BLAST21
Transmembranei525 – 545HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002015891 – 552Cytochrome c-type biogenesis protein NrfEAdd BLAST552

Proteomic databases

PaxDbiP32710.
PRIDEiP32710.

Interactioni

Protein-protein interaction databases

BioGridi4262677. 7 interactors.
STRINGi511145.b4074.

Structurei

3D structure databases

ProteinModelPortaliP32710.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108QKW. Bacteria.
COG1138. LUCA.
HOGENOMiHOG000274659.
InParanoidiP32710.
KOiK04016.
OMAiIPERRYY.
PhylomeDBiP32710.

Family and domain databases

InterProiIPR032523. CcmF_C.
IPR002541. Cyt_c_assembly.
IPR003567. Cyt_c_biogenesis.
IPR003568. Cyt_c_biogenesis_CcmF.
IPR003570. Cyt_c_biogenesis_NrfE.
[Graphical view]
PfamiPF16327. CcmF_C. 2 hits.
PF01578. Cytochrom_C_asm. 1 hit.
[Graphical view]
PRINTSiPR01410. CCBIOGENESIS.
PR01413. NRFEBIOGNSIS.
TIGRFAMsiTIGR00353. nrfE. 1 hit.

Sequencei

Sequence statusi: Complete.

P32710-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTPLTAFAG VRLRWPAMMR LTCIGILAQF ALLLLAFGVL TYCFLISDFS
60 70 80 90 100
VIYVAQHSYS LLSWELKLAA VWGGHEGSLL LWVLLLSAWS ALFAWHYRQQ
110 120 130 140 150
TDPLFPLTLA VLSLMLAALL LFVVLWSDPF VRIFPPAIEG RDLNPMLQHP
160 170 180 190 200
GLIFHPPLLY LGYGGLMVAA SVALASLLRG EFDGACARIC WRWALPGWSA
210 220 230 240 250
LTAGIILGSW WAYCELGWGG WWFWDPVENA SLLPWLSATA LLHSLSLTRQ
260 270 280 290 300
RGIFCHWSLL LAIVTLMLSL LGTLIVRSGI LVSVHAFALD NVRAVPLFSL
310 320 330 340 350
FALISLASLA LYGWRARDGG PAVHFSGLSR EMLILATLLL FCAVLLIVLV
360 370 380 390 400
GTLYPMIYGL LGWGRLSVGA PYFNRATLPF GLLMLVVIVL ATFVSGKRVQ
410 420 430 440 450
LPALVAHAGV LLFAAGVVVS SVSRQEISLN LQPGQQVTLA GYTFRFECLD
460 470 480 490 500
LQAKGNYTSE KAIVALFDHQ QRIGELTPER RFYEARRQQM MEPSIRWNGI
510 520 530 540 550
HDWYAVMGEK TGPDRYAFRL YVQSGVRWIW GGGLLMIAGA LLSGWRGKKR

DE
Length:552
Mass (Da):61,295
Last modified:August 29, 2003 - v2
Checksum:i138C2E21933699E3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32L → V in CAA51045 (PubMed:8057835).Curated1
Sequence conflicti38G → A in CAA51045 (PubMed:8057835).Curated1
Sequence conflicti190C → S in CAA51045 (PubMed:8057835).Curated1
Sequence conflicti218W → L (PubMed:8057835).Curated1
Sequence conflicti220 – 226GWWFWDP → RLVVLGS (PubMed:8057835).Curated7
Sequence conflicti455G → A in CAA51045 (PubMed:8057835).Curated1
Sequence conflicti481R → A in CAA51045 (PubMed:8057835).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72298 Genomic DNA. Translation: CAA51045.1.
U00006 Genomic DNA. Translation: AAC43168.1.
U00096 Genomic DNA. Translation: AAD13457.1.
AP009048 Genomic DNA. Translation: BAE78076.1.
PIRiA65216. E57987.
RefSeqiNP_418498.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAD13457; AAD13457; b4074.
BAE78076; BAE78076; BAE78076.
GeneIDi948579.
KEGGiecj:JW4035.
eco:b4074.
PATRICi32123697. VBIEscCol129921_4197.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72298 Genomic DNA. Translation: CAA51045.1.
U00006 Genomic DNA. Translation: AAC43168.1.
U00096 Genomic DNA. Translation: AAD13457.1.
AP009048 Genomic DNA. Translation: BAE78076.1.
PIRiA65216. E57987.
RefSeqiNP_418498.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP32710.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262677. 7 interactors.
STRINGi511145.b4074.

Proteomic databases

PaxDbiP32710.
PRIDEiP32710.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD13457; AAD13457; b4074.
BAE78076; BAE78076; BAE78076.
GeneIDi948579.
KEGGiecj:JW4035.
eco:b4074.
PATRICi32123697. VBIEscCol129921_4197.

Organism-specific databases

EchoBASEiEB1891.
EcoGeneiEG11948. nrfE.

Phylogenomic databases

eggNOGiENOG4108QKW. Bacteria.
COG1138. LUCA.
HOGENOMiHOG000274659.
InParanoidiP32710.
KOiK04016.
OMAiIPERRYY.
PhylomeDBiP32710.

Enzyme and pathway databases

BioCyciEcoCyc:EG11948-MONOMER.
ECOL316407:JW4035-MONOMER.

Miscellaneous databases

PROiP32710.

Family and domain databases

InterProiIPR032523. CcmF_C.
IPR002541. Cyt_c_assembly.
IPR003567. Cyt_c_biogenesis.
IPR003568. Cyt_c_biogenesis_CcmF.
IPR003570. Cyt_c_biogenesis_NrfE.
[Graphical view]
PfamiPF16327. CcmF_C. 2 hits.
PF01578. Cytochrom_C_asm. 1 hit.
[Graphical view]
PRINTSiPR01410. CCBIOGENESIS.
PR01413. NRFEBIOGNSIS.
TIGRFAMsiTIGR00353. nrfE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNRFE_ECOLI
AccessioniPrimary (citable) accession number: P32710
Secondary accession number(s): Q2M6N0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: August 29, 2003
Last modified: November 2, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.