P32709 (NRFD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein NrfD

Gene names

Name:	nrfD
Synonyms:	yjcK
Ordered Locus Names:	b4073, JW4034

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.5.
Sequence similarities	Belongs to the NrfD family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Protein NrfD		PRO_0000159322
Regions
	Transmembrane	18 – 38	21	Helical; Potential
	Transmembrane	57 – 73	17	Helical; Potential
	Transmembrane	90 – 112	23	Helical; Potential
	Transmembrane	150 – 170	21	Helical; Potential
	Transmembrane	180 – 199	20	Helical; Potential
	Transmembrane	222 – 242	21	Helical; Potential
	Transmembrane	258 – 278	21	Helical; Potential
	Transmembrane	288 – 310	23	Helical; Potential
Experimental info
	Sequence conflict	141	1	L → V in CAA51044. Ref.1
	Sequence conflict	202	1	A → R in CAA51044. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32709 [UniParc]. Last modified August 29, 2003. Version 2. Checksum: B650FB208C4C4FEE	FASTA	318	35,042
	10 20 30 40 50 60 MTQTSAFHFE SLVWDWPIAI YLFLIGISAG LVTLAVLLRR FYPQAGGADS TLLRTTLIVG 70 80 90 100 110 120 PGAVILGLLI LVFHLTRPWT FWKLMFHYSF TSVMSMGVML FQLYMVVLVL WLAKIFEHDL 130 140 150 160 170 180 LALQQRWLPK LGIVQKVLSL LTPVHRGLET LMLVLAVLLG AYTGFLLSAL KSYPFLNNPI 190 200 210 220 230 240 LPVLFLFSGI SSGAAVALIA MAIRQRSNPH STEAQFVHRM EIPVVWGEIF LLVAFFVGLA 250 260 270 280 290 300 LGDDGKVRAL VAALGGGFWT WWFWLGVAGL GLIVPMLLKP WVNRSSGIPA VLAACGASLV 310 GVLMLRFFIL YAGQLTVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A seven-gene operon essential for formate-dependent nitrite reduction to ammonia by enteric bacteria." Hussain H.A., Grove J., Griffiths L., Busby S., Cole J. Mol. Microbiol. 12:153-163(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X72298 Genomic DNA. Translation: CAA51044.1. U00006 Genomic DNA. Translation: AAC43167.1. U00096 Genomic DNA. Translation: AAC77043.1. AP009048 Genomic DNA. Translation: BAE78075.1.
PIR	D57987. H65215.
RefSeq	NP_418497.1. NC_000913.2. YP_492216.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P32709.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b4073.
Proteomic databases
PRIDE	P32709.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC77043; AAC77043; b4073. BAE78075; BAE78075; BAE78075.
GeneID	12933682. 948580.
KEGG	ecj:Y75_p3960. eco:b4073.
PATRIC	32123695. VBIEscCol129921_4196.
Organism-specific databases
EchoBASE	EB1890.
EcoGene	EG11947. nrfD.
Phylogenomic databases
eggNOG	COG3301.
HOGENOM	HOG000278536.
KO	K04015.
OMA	PIDAWCE.
ProtClustDB	CLSK869938.
Enzyme and pathway databases
BioCyc	EcoCyc:NRFD-MONOMER. ECOL316407:JW4034-MONOMER. MetaCyc:NRFD-MONOMER.
Gene expression databases
Genevestigator	P32709.
Family and domain databases
InterPro	IPR017566. NrfD. IPR005614. NrfD_fam. [Graphical view]
Pfam	PF03916. NrfD. 1 hit. [Graphical view]
TIGRFAMs	TIGR03148. cyt_nit_nrfD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NRFD_ECOLI

Accession

Primary (citable) accession number: P32709
Secondary accession number(s): Q2M6N1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	August 29, 2003
Last modified:	May 1, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program