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Protein

Cation/acetate symporter ActP

Gene

actP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transports acetate. Also able to transport glycolate.1 Publication

Kineticsi

  1. KM=5.4 µM for acetate1 Publication
  1. Vmax=19.6 nmol/min/mg enzyme1 Publication

GO - Molecular functioni

GO - Biological processi

  • plasma membrane acetate transport Source: EcoCyc
  • sodium ion transport Source: UniProtKB-KW
  • tellurite transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

BioCyciEcoCyc:YJCG-MONOMER.
ECOL316407:JW4028-MONOMER.
MetaCyc:YJCG-MONOMER.

Protein family/group databases

TCDBi2.A.21.7.2. the solute:sodium symporter (sss) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Cation/acetate symporter ActP
Alternative name(s):
Acetate permease
Acetate transporter ActP
Gene namesi
Name:actP
Synonyms:yjcG
Ordered Locus Names:b4067, JW4028
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11942. actP.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3232PeriplasmicSequence analysisAdd
BLAST
Transmembranei33 – 5523HelicalSequence analysisAdd
BLAST
Topological domaini56 – 7520CytoplasmicSequence analysisAdd
BLAST
Transmembranei76 – 9823HelicalSequence analysisAdd
BLAST
Topological domaini99 – 1024PeriplasmicSequence analysis
Transmembranei103 – 12523HelicalSequence analysisAdd
BLAST
Topological domaini126 – 14520CytoplasmicSequence analysisAdd
BLAST
Transmembranei146 – 16823HelicalSequence analysisAdd
BLAST
Topological domaini169 – 18214PeriplasmicSequence analysisAdd
BLAST
Transmembranei183 – 20523HelicalSequence analysisAdd
BLAST
Topological domaini206 – 2116CytoplasmicSequence analysis
Transmembranei212 – 23423HelicalSequence analysisAdd
BLAST
Topological domaini235 – 26026PeriplasmicSequence analysisAdd
BLAST
Transmembranei261 – 28323HelicalSequence analysisAdd
BLAST
Topological domaini284 – 30219CytoplasmicSequence analysisAdd
BLAST
Transmembranei303 – 32523HelicalSequence analysisAdd
BLAST
Topological domaini326 – 35833PeriplasmicSequence analysisAdd
BLAST
Transmembranei359 – 38123HelicalSequence analysisAdd
BLAST
Topological domaini382 – 40120CytoplasmicSequence analysisAdd
BLAST
Transmembranei402 – 42423HelicalSequence analysisAdd
BLAST
Topological domaini425 – 4273PeriplasmicSequence analysis
Transmembranei428 – 45023HelicalSequence analysisAdd
BLAST
Topological domaini451 – 46111CytoplasmicSequence analysisAdd
BLAST
Transmembranei462 – 48423HelicalSequence analysisAdd
BLAST
Topological domaini485 – 4939PeriplasmicSequence analysis
Transmembranei494 – 51623HelicalSequence analysisAdd
BLAST
Topological domaini517 – 54933CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 549549Cation/acetate symporter ActPPRO_0000105407Add
BLAST

Proteomic databases

EPDiP32705.
PaxDbiP32705.

Interactioni

Subunit structurei

Has been isolated from inner membrane preparations as a homodimer.1 Publication

Protein-protein interaction databases

BioGridi4259431. 12 interactions.
DIPiDIP-12552N.
IntActiP32705. 1 interaction.
MINTiMINT-1259884.
STRINGi511145.b4067.

Structurei

3D structure databases

ProteinModelPortaliP32705.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107QN1. Bacteria.
COG4147. LUCA.
HOGENOMiHOG000242183.
InParanoidiP32705.
KOiK14393.
OMAiCAYALFI.
PhylomeDBiP32705.

Family and domain databases

HAMAPiMF_01426. Acet_symport_ActP. 1 hit.
InterProiIPR014083. Cation/Ac_symporter_ActP.
IPR001734. Na/solute_symporter.
IPR018212. Na/solute_symporter_CS.
[Graphical view]
PANTHERiPTHR11819. PTHR11819. 1 hit.
PfamiPF00474. SSF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00813. sss. 1 hit.
TIGR02711. symport_actP. 1 hit.
PROSITEiPS00456. NA_SOLUT_SYMP_1. 1 hit.
PS00457. NA_SOLUT_SYMP_2. 1 hit.
PS50283. NA_SOLUT_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32705-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRVLTALAA TLPFAANAAD AISGAVERQP TNWQAIIMFL IFVVFTLGIT
60 70 80 90 100
YWASKRVRSR SDYYTAGGNI TGFQNGLAIA GDYMSAASFL GISALVFTSG
110 120 130 140 150
YDGLIYSLGF LVGWPIILFL IAERLRNLGR YTFADVASYR LKQGPIRILS
160 170 180 190 200
ACGSLVVVAL YLIAQMVGAG KLIELLFGLN YHIAVVLVGV LMMMYVLFGG
210 220 230 240 250
MLATTWVQII KAVLLLFGAS FMAFMVMKHV GFSFNNLFSE AMAVHPKGVD
260 270 280 290 300
IMKPGGLVKD PISALSLGLG LMFGTAGLPH ILMRFFTVSD AREARKSVFY
310 320 330 340 350
ATGFMGYFYI LTFIIGFGAI MLVGANPEYK DAAGHLIGGN NMAAVHLANA
360 370 380 390 400
VGGNLFLGFI SAVAFATILA VVAGLTLAGA SAVSHDLYAN VFKKGATERE
410 420 430 440 450
ELRVSKITVL ILGVIAIILG VLFENQNIAF MVGLAFAIAA SCNFPIILLS
460 470 480 490 500
MYWSKLTTRG AMMGGWLGLI TAVVLMILGP TIWVQILGHE KAIFPYEYPA
510 520 530 540
LFSITVAFLG IWFFSATDNS AEGARERELF RAQFIRSQTG FGVEQGRAH
Length:549
Mass (Da):59,197
Last modified:October 1, 1993 - v1
Checksum:iE7E116FB919D0FFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43161.1.
U00096 Genomic DNA. Translation: AAC77037.1.
AP009048 Genomic DNA. Translation: BAE78069.1.
PIRiB65215.
RefSeqiNP_418491.1. NC_000913.3.
WP_000832573.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77037; AAC77037; b4067.
BAE78069; BAE78069; BAE78069.
GeneIDi948575.
KEGGiecj:JW4028.
eco:b4067.
PATRICi32123683. VBIEscCol129921_4190.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43161.1.
U00096 Genomic DNA. Translation: AAC77037.1.
AP009048 Genomic DNA. Translation: BAE78069.1.
PIRiB65215.
RefSeqiNP_418491.1. NC_000913.3.
WP_000832573.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP32705.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259431. 12 interactions.
DIPiDIP-12552N.
IntActiP32705. 1 interaction.
MINTiMINT-1259884.
STRINGi511145.b4067.

Protein family/group databases

TCDBi2.A.21.7.2. the solute:sodium symporter (sss) family.

Proteomic databases

EPDiP32705.
PaxDbiP32705.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77037; AAC77037; b4067.
BAE78069; BAE78069; BAE78069.
GeneIDi948575.
KEGGiecj:JW4028.
eco:b4067.
PATRICi32123683. VBIEscCol129921_4190.

Organism-specific databases

EchoBASEiEB1886.
EcoGeneiEG11942. actP.

Phylogenomic databases

eggNOGiENOG4107QN1. Bacteria.
COG4147. LUCA.
HOGENOMiHOG000242183.
InParanoidiP32705.
KOiK14393.
OMAiCAYALFI.
PhylomeDBiP32705.

Enzyme and pathway databases

BioCyciEcoCyc:YJCG-MONOMER.
ECOL316407:JW4028-MONOMER.
MetaCyc:YJCG-MONOMER.

Miscellaneous databases

PROiP32705.

Family and domain databases

HAMAPiMF_01426. Acet_symport_ActP. 1 hit.
InterProiIPR014083. Cation/Ac_symporter_ActP.
IPR001734. Na/solute_symporter.
IPR018212. Na/solute_symporter_CS.
[Graphical view]
PANTHERiPTHR11819. PTHR11819. 1 hit.
PfamiPF00474. SSF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00813. sss. 1 hit.
TIGR02711. symport_actP. 1 hit.
PROSITEiPS00456. NA_SOLUT_SYMP_1. 1 hit.
PS00457. NA_SOLUT_SYMP_2. 1 hit.
PS50283. NA_SOLUT_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACTP_ECOLI
AccessioniPrimary (citable) accession number: P32705
Secondary accession number(s): Q2M6N7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 7, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Although ActP belongs to the sodium:solute symporter family, the experiments did not allow to show that acetate transport depends on sodium. The inhibition of acetate transport by the uncoupler CCCP indicates that the driving force used by ActP is a transmembrane electrochemical potential. However, it does not allow to discriminate between hydrogen ion or sodium ion-coupled transporters (PubMed:14563880).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.