Cation/acetate symporter ActP - Escherichia coli (strain K12)

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P32705 (ACTP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cation/acetate symporter ActP

Alternative name(s):
Acetate permease
Acetate transporter ActP

Gene names

Name:	actP
Synonyms:	yjcG
Ordered Locus Names:	b4067, JW4028

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transports acetate. Also able to transport glycolate. HAMAP MF_01426
Subunit structure	Has been isolated from inner membrane preparations as a homodimer. Ref.5
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.5.
Miscellaneous	Although ActP belongs to the sodium:solute symporter family, the experiments did not allow to show that acetate transport depends on sodium. The inhibition of acetate transport by the uncoupler CCCP indicates that the driving force used by ActP is a transmembrane electrochemical potential. However, it does not allow to discriminate between hydrogen ion or sodium ion-coupled transporters. HAMAP MF_01426
Sequence similarities	Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. [View classification]
Biophysicochemical properties	Kinetic parameters: K_M=5.4 µM for acetate V_max=19.6 nmol/min/mg enzyme

Ontologies

Keywords
Biological process	Ion transport Sodium transport Symport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Sodium
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	sodium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to plasma membrane Inferred from electronic annotation. Source: InterPro
Molecular function	symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 549

549

Cation/acetate symporter ActP HAMAP MF_01426

PRO_0000105407

Regions

Topological domain

1 – 32

Periplasmic Potential

Transmembrane

33 – 55

Helical; Potential

Topological domain

56 – 75

Cytoplasmic Potential

Transmembrane

76 – 98

Helical; Potential

Topological domain

99 – 102

Periplasmic Potential

Transmembrane

103 – 125

Helical; Potential

Topological domain

126 – 145

Cytoplasmic Potential

Transmembrane

146 – 168

Helical; Potential

Topological domain

169 – 182

Periplasmic Potential

Transmembrane

183 – 205

Helical; Potential

Topological domain

206 – 211

Cytoplasmic Potential

Transmembrane

212 – 234

Helical; Potential

Topological domain

235 – 260

Periplasmic Potential

Transmembrane

261 – 283

Helical; Potential

Topological domain

284 – 302

Cytoplasmic Potential

Transmembrane

303 – 325

Helical; Potential

Topological domain

326 – 358

Periplasmic Potential

Transmembrane

359 – 381

Helical; Potential

Topological domain

382 – 401

Cytoplasmic Potential

Transmembrane

402 – 424

Helical; Potential

Topological domain

425 – 427

Periplasmic Potential

Transmembrane

428 – 450

Helical; Potential

Topological domain

451 – 461

Cytoplasmic Potential

Transmembrane

462 – 484

Helical; Potential

Topological domain

485 – 493

Periplasmic Potential

Transmembrane

494 – 516

Helical; Potential

Topological domain

517 – 549

Cytoplasmic Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P32705 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: E7E116FB919D0FFD
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FASTA

549

59,197

        10         20         30         40         50         60 
MKRVLTALAA TLPFAANAAD AISGAVERQP TNWQAIIMFL IFVVFTLGIT YWASKRVRSR 

        70         80         90        100        110        120 
SDYYTAGGNI TGFQNGLAIA GDYMSAASFL GISALVFTSG YDGLIYSLGF LVGWPIILFL 

       130        140        150        160        170        180 
IAERLRNLGR YTFADVASYR LKQGPIRILS ACGSLVVVAL YLIAQMVGAG KLIELLFGLN 

       190        200        210        220        230        240 
YHIAVVLVGV LMMMYVLFGG MLATTWVQII KAVLLLFGAS FMAFMVMKHV GFSFNNLFSE 

       250        260        270        280        290        300 
AMAVHPKGVD IMKPGGLVKD PISALSLGLG LMFGTAGLPH ILMRFFTVSD AREARKSVFY 

       310        320        330        340        350        360 
ATGFMGYFYI LTFIIGFGAI MLVGANPEYK DAAGHLIGGN NMAAVHLANA VGGNLFLGFI 

       370        380        390        400        410        420 
SAVAFATILA VVAGLTLAGA SAVSHDLYAN VFKKGATERE ELRVSKITVL ILGVIAIILG 

       430        440        450        460        470        480 
VLFENQNIAF MVGLAFAIAA SCNFPIILLS MYWSKLTTRG AMMGGWLGLI TAVVLMILGP 

       490        500        510        520        530        540 
TIWVQILGHE KAIFPYEYPA LFSITVAFLG IWFFSATDNS AEGARERELF RAQFIRSQTG 


FGVEQGRAH

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The gene yjcG, cotranscribed with the gene acs, encodes an acetate permease in Escherichia coli." Gimenez R., Nunez M.F., Badia J., Aguilar J., Baldoma L. J. Bacteriol. 185:6448-6455(2003) [PubMed: 14563880] [Abstract] Cited for: CHARACTERIZATION. Strain: K12.
[5]	"Protein complexes of the Escherichia coli cell envelope." Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L., von Heijne G., Daley D.O. J. Biol. Chem. 280:34409-34419(2005) [PubMed: 16079137] [Abstract] Cited for: SUBUNIT, SUBCELLULAR LOCATION. Strain: BL21-DE3.
[6]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00006 Genomic DNA. Translation: AAC43161.1. U00096 Genomic DNA. Translation: AAC77037.1. AP009048 Genomic DNA. Translation: BAE78069.1.
PIR	B65215.
RefSeq	NP_418491.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P32705.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-12552N.
IntAct	P32705. 2 interactions.
MINT	MINT-1259884.
Protein family/group databases
TCDB	2.A.21.7.2. solute:sodium symporter (SSS) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001078; EBESCP00000001078; EBESCG00000000893. EBESCT00000001079; EBESCP00000001079; EBESCG00000000893. EBESCT00000016835; EBESCP00000016126; EBESCG00000015894.
GeneID	948575.
GenomeReviews	Gene locus JW4028 in contig AP009048_GR. Gene locus b4067 in contig U00096_GR.
KEGG	ecj:JW4028. eco:b4067.
PATRIC	32123683. VBIEscCol129921_4190.
Organism-specific databases
EchoBASE	EB1886.
EcoGene	EG11942. actP.
Phylogenomic databases
eggNOG	COG4147.
GeneTree	EBGT00050000009742.
HOGENOM	HBG533958.
OMA	LYASVWR.
PhylomeDB	P32705.
ProtClustDB	PRK09395.
Enzyme and pathway databases
BioCyc	EcoCyc:YJCG-MONOMER.
Gene expression databases
Genevestigator	P32705.
Family and domain databases
HAMAP	MF_01426. Acet_symport_ActP. [Tree]
InterPro	IPR014083. Cation/Ac_symporter_ActP. IPR001734. Na/solute_symporter. IPR018212. Na/solute_symporter_CS. IPR019900. Na/solute_symporter_subgr. [Graphical view]
KO	K14393.
PANTHER	PTHR11819. Na/solut_symport. 1 hit.
Pfam	PF00474. SSF. 1 hit. [Graphical view]
TIGRFAMs	TIGR00813. Sss. 1 hit. TIGR02711. Symport_actP. 1 hit.
PROSITE	PS00456. NA_SOLUT_SYMP_1. 1 hit. PS00457. NA_SOLUT_SYMP_2. 1 hit. PS50283. NA_SOLUT_SYMP_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACTP_ECOLI

Accession

Primary (citable) accession number: P32705
Secondary accession number(s): Q2M6N7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	January 25, 2012
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents