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Reviewed, UniProtKB/Swiss-Prot P32705 (ACTP_ECOLI)

Last modified June 16, 2009. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cation/acetate symporter actP
Alternative name(s):
    Acetate transporter actP
    Acetate permease
Gene names
Name: actP
Synonyms: yjcG
Ordered Locus Names: b4067, JW4028
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length549 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Transports acetate. Also able to transport glycolate. HAMAP MF_01426

Subunit structure

Has been isolated from inner membrane preparations as a homodimer. Ref.5

Subcellular location

Cell inner membrane; Multi-pass membrane protein. Ref.5

Miscellaneous

Although actP belongs to the sodium:solute symporter family, the experiments did not allow to show that acetate transport depends on sodium. The inhibition of acetate transport by the uncoupler CCCP indicates that the driving force used by actP is a transmembrane electrochemical potential. However, it does not allow to discriminate between hydrogen ion or sodium ion-coupled transporters. HAMAP MF_01426

Sequence similarities

Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. [View classification]

biophysicochemical properties

Kinetic parameters:

KM=5.4 µM for acetate HAMAP MF_01426

Vmax=19.6 nmol/min/mg enzyme

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Ontologies

Keywords
   Biological processIon transport
Sodium transport
Symport
Transport
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
   LigandSodium
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processsodium ion transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionacetate transmembrane transporter activity

Inferred from electronic annotation. Source: HAMAP

sodium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

symporter activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 549549Cation/acetate symporter actP HAMAP MF_01426
PRO_0000105407

Regions

Topological domain1 – 3232Periplasmic Potential
Transmembrane33 – 5523 Potential
Topological domain56 – 7520Cytoplasmic Potential
Transmembrane76 – 9823 Potential
Topological domain99 – 1024Periplasmic Potential
Transmembrane103 – 12523 Potential
Topological domain126 – 14520Cytoplasmic Potential
Transmembrane146 – 16823 Potential
Topological domain169 – 18214Periplasmic Potential
Transmembrane183 – 20523 Potential
Topological domain206 – 2116Cytoplasmic Potential
Transmembrane212 – 23423 Potential
Topological domain235 – 26026Periplasmic Potential
Transmembrane261 – 28323 Potential
Topological domain284 – 30219Cytoplasmic Potential
Transmembrane303 – 32523 Potential
Topological domain326 – 35833Periplasmic Potential
Transmembrane359 – 38123 Potential
Topological domain382 – 40120Cytoplasmic Potential
Transmembrane402 – 42423 Potential
Topological domain425 – 4273Periplasmic Potential
Transmembrane428 – 45023 Potential
Topological domain451 – 46111Cytoplasmic Potential
Transmembrane462 – 48423 Potential
Topological domain485 – 4939Periplasmic Potential
Transmembrane494 – 51623 Potential
Topological domain517 – 54933Cytoplasmic Potential

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Sequences

Sequence LengthMass (Da)Tools
P32705-1 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: E7E116FB919D0FFD

FASTA54959,197
        10         20         30         40         50         60 
MKRVLTALAA TLPFAANAAD AISGAVERQP TNWQAIIMFL IFVVFTLGIT YWASKRVRSR 

        70         80         90        100        110        120 
SDYYTAGGNI TGFQNGLAIA GDYMSAASFL GISALVFTSG YDGLIYSLGF LVGWPIILFL 

       130        140        150        160        170        180 
IAERLRNLGR YTFADVASYR LKQGPIRILS ACGSLVVVAL YLIAQMVGAG KLIELLFGLN 

       190        200        210        220        230        240 
YHIAVVLVGV LMMMYVLFGG MLATTWVQII KAVLLLFGAS FMAFMVMKHV GFSFNNLFSE 

       250        260        270        280        290        300 
AMAVHPKGVD IMKPGGLVKD PISALSLGLG LMFGTAGLPH ILMRFFTVSD AREARKSVFY 

       310        320        330        340        350        360 
ATGFMGYFYI LTFIIGFGAI MLVGANPEYK DAAGHLIGGN NMAAVHLANA VGGNLFLGFI 

       370        380        390        400        410        420 
SAVAFATILA VVAGLTLAGA SAVSHDLYAN VFKKGATERE ELRVSKITVL ILGVIAIILG 

       430        440        450        460        470        480 
VLFENQNIAF MVGLAFAIAA SCNFPIILLS MYWSKLTTRG AMMGGWLGLI TAVVLMILGP 

       490        500        510        520        530        540 
TIWVQILGHE KAIFPYEYPA LFSITVAFLG IWFFSATDNS AEGARERELF RAQFIRSQTG 


FGVEQGRAH

« Hide

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References

« Hide 'large scale' references
[1]"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The gene yjcG, cotranscribed with the gene acs, encodes an acetate permease in Escherichia coli."
Gimenez R., Nunez M.F., Badia J., Aguilar J., Baldoma L.
J. Bacteriol. 185:6448-6455(2003) [PubMed: 14563880] [Abstract]
Cited for: CHARACTERIZATION.
Strain: K12.
[5]"Protein complexes of the Escherichia coli cell envelope."
Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L., von Heijne G., Daley D.O.
J. Biol. Chem. 280:34409-34419(2005) [PubMed: 16079137] [Abstract]
Cited for: SUBUNIT, SUBCELLULAR LOCATION.
Strain: BL21-DE3.
[6]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.

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Cross-references

Sequence databases

U00006 Genomic DNA. Translation: AAC43161.1.
U00096 Genomic DNA. Translation: AAC77037.1.
AP009048 Genomic DNA. Translation: BAE78069.1.
PIRB65215.
RefSeqAP_004568.1.
NP_418491.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:12552N.
IntActP32705. 2 interactions.

Protein family/group databases

TCDB2.A.21.7.2. solute:sodium symporter (SSS) family.

Genome annotation databases

GeneID948575.
GenomeReviewsGene locus JW4028 in contig AP009048_GR.
Gene locus b4067 in contig U00096_GR.
KEGGecj:JW4028.
eco:b4067.

Organism-specific databases

EchoBASEEB1886.
EcoGeneEG11942. actP.
CMRSearch...

Phylogenomic databases

HOGENOMP32705.
OMAP32705. PQFVRSQ.

Enzyme and pathway databases

BioCycEcoCyc:YJCG-MON.

Family and domain databases

HAMAPMF_01426.
[Tree]
InterProIPR014083. Cation/Ac_symporter_ActP.
IPR001734. Na/solute_symporter.
IPR018212. Na/solute_symporter_CS.
IPR019900. Na/solute_symporter_subgr.
[Graphical view]
PANTHERPTHR11819. Na/solut_symport. 1 hit.
PfamPF00474. SSF. 1 hit.
[Graphical view]
TIGRFAMsTIGR00813. sss. 1 hit.
TIGR02711. symport_actP. 1 hit.
PROSITEPS00456. NA_SOLUT_SYMP_1. 1 hit.
PS00457. NA_SOLUT_SYMP_2. 1 hit.
PS50283. NA_SOLUT_SYMP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameACTP_ECOLI
AccessionPrimary (citable) accession number: P32705
Secondary accession number(s): Q2M6N7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents