P32695 (DUSA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-dihydrouridine synthase A
EC=1.-.-.-

Gene names

Name:	dusA
Synonyms:	yjbN
Ordered Locus Names:	b4049, JW5950

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Ref.4
Cofactor	FAD By similarity.
Sequence similarities	Belongs to the dus family. DusA subfamily.
Sequence caution	The sequence AAC43143.1 differs from that shown. Reason: Erroneous initiation. The sequence BAE78051.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro tRNA dihydrouridine synthase activity Inferred from mutant phenotype Ref.4. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	tRNA-dihydrouridine synthase A		PRO_0000162063

Sequences

	Sequence		Length	Mass (Da)
	P32695 [UniParc]. Last modified July 15, 1998. Version 3. Checksum: 8C4196ADED1E9C68	FASTA	330	36,810
	10 20 30 40 50 60 MPEKTDVHWS GRFSVAPMLD WTDRHCRYFL RLLSRNTLLY TEMVTTGAII HGKGDYLAYS 70 80 90 100 110 120 EEEHPVALQL GGSDPAALAQ CAKLAEARGY DEINLNVGCP SDRVQNGMFG ACLMGNAQLV 130 140 150 160 170 180 ADCVKAMRDV VSIPVTVKTR IGIDDQDSYE FLCDFINTVS GKGECEMFII HARKAWLSGL 190 200 210 220 230 240 SPKENREIPP LDYPRVYQLK RDFPHLTMSI NGGIKSLEEA KAHLQHMDGV MVGREAYQNP 250 260 270 280 290 300 GILAAVDREI FGSSDTDADP VAVVRAMYPY IERELSQGTY LGHITRHMLG LFQGIPGARQ 310 320 330 WRRYLSENAH KAGADINVLE HALKLVADKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Identification of the tRNA-dihydrouridine synthase family." Bishop A.C., Xu J., Johnson R.C., Schimmel P., de Crecy-Lagard V. J. Biol. Chem. 277:25090-25095(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: K12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00006 Genomic DNA. Translation: AAC43143.1. Different initiation. U00096 Genomic DNA. Translation: AAC77019.2. AP009048 Genomic DNA. Translation: BAE78051.1. Different initiation.
RefSeq	NP_418473.2. NC_000913.2. YP_492192.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P32695.
SMR	P32695. Positions 12-326.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b4049.
Proteomic databases
PaxDb	P32695.
PRIDE	P32695.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC77019; AAC77019; b4049. BAE78051; BAE78051; BAE78051.
GeneID	12930614. 948558.
KEGG	ecj:Y75_p3936. eco:b4049.
PATRIC	32123637. VBIEscCol129921_4167.
Organism-specific databases
EchoBASE	EB1876.
EcoGene	EG11932. dusA.
Phylogenomic databases
eggNOG	COG0042.
HOGENOM	HOG000259834.
KO	K05539.
OMA	IPPLDYD.
ProtClustDB	PRK11815.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11932-MONOMER. ECOL316407:JW5950-MONOMER. MetaCyc:EG11932-MONOMER.
Gene expression databases
Genevestigator	P32695.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR004653. tRNA_dU_YjbN. IPR001269. tRNA_hU_synthase. IPR018517. tRNA_hU_synthase_CS. [Graphical view]
PANTHER	PTHR11082. PTHR11082. 1 hit.
Pfam	PF01207. Dus. 1 hit. [Graphical view]
PIRSF	PIRSF006621. Dus. 1 hit.
TIGRFAMs	TIGR00742. yjbN. 1 hit.
PROSITE	PS01136. UPF0034. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DUSA_ECOLI

Accession

Primary (citable) accession number: P32695
Secondary accession number(s): P76786, Q2M6Q5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	July 15, 1998
Last modified:	May 1, 2013
This is version 97 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program