Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

23S rRNA pseudouridine(2604) synthase

Gene

rluF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of pseudouridine from uracil-2604 in 23S ribosomal RNA. Can, to a small extent, also react with uracil-2605.1 Publication

Catalytic activityi

23S rRNA uridine(2604) = 23S rRNA pseudouridine(2604).2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei107 – 1071Nucleophile1 Publication

GO - Molecular functioni

  • pseudouridine synthase activity Source: EcoCyc
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • enzyme-directed rRNA pseudouridine synthesis Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11921-MONOMER.
ECOL316407:JW3982-MONOMER.
MetaCyc:EG11921-MONOMER.
BRENDAi5.4.99.21. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
23S rRNA pseudouridine(2604) synthase1 Publication (EC:5.4.99.212 Publications)
Alternative name(s):
Ribosomal large subunit pseudouridine synthase F
rRNA pseudouridylate synthase F
rRNA-uridine isomerase F
Gene namesi
Name:rluF
Synonyms:yjbC
Ordered Locus Names:b4022, JW3982
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11921. rluF.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi107 – 1071D → N or T: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 29029023S rRNA pseudouridine(2604) synthasePRO_0000100016Add
BLAST

Proteomic databases

PaxDbiP32684.
PRIDEiP32684.

Interactioni

Subunit structurei

Monomer.2 Publications

Protein-protein interaction databases

BioGridi4261777. 6 interactions.
IntActiP32684. 9 interactions.
STRINGi511145.b4022.

Structurei

Secondary structure

1
290
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 146Combined sources
Turni15 – 173Combined sources
Helixi21 – 299Combined sources
Beta strandi33 – 353Combined sources
Beta strandi52 – 543Combined sources
Beta strandi57 – 593Combined sources
Helixi64 – 663Combined sources
Beta strandi69 – 746Combined sources
Beta strandi82 – 854Combined sources
Beta strandi87 – 893Combined sources
Helixi90 – 934Combined sources
Beta strandi101 – 1044Combined sources
Beta strandi111 – 1188Combined sources
Helixi120 – 13213Combined sources
Beta strandi135 – 1439Combined sources
Helixi147 – 1537Combined sources
Beta strandi160 – 1634Combined sources
Beta strandi168 – 1736Combined sources
Beta strandi176 – 1816Combined sources
Helixi188 – 1958Combined sources
Beta strandi200 – 2089Combined sources
Beta strandi221 – 2233Combined sources
Helixi226 – 2338Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GMLX-ray2.60A/B66-290[»]
3DH3X-ray3.00A/B/C/D1-290[»]
ProteinModelPortaliP32684.
SMRiP32684. Positions 3-243.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32684.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 7266S4 RNA-bindingPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni105 – 1084Interaction with RNA1 Publication
Regioni187 – 1904Interaction with RNA1 Publication

Sequence similaritiesi

Belongs to the pseudouridine synthase RsuA family.Curated
Contains 1 S4 RNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105FE9. Bacteria.
COG1187. LUCA.
HOGENOMiHOG000044954.
InParanoidiP32684.
KOiK06182.
OMAiCEYLGYE.
PhylomeDBiP32684.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR000748. PsdUridine_synth_RsuA/RluB/E/F.
IPR018496. PsdUridine_synth_RsuA/RluB_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
IPR002942. S4_RNA-bd.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
SMARTiSM00363. S4. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00093. TIGR00093. 1 hit.
PROSITEiPS01149. PSI_RSU. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32684-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPDSSVRLN KYISESGICS RREADRYIEQ GNVFLNGKRA TIGDQVKPGD
60 70 80 90 100
VVKVNGQLIE PREAEDLVLI ALNKPVGIVS TTEDGERDNI VDFVNHSKRV
110 120 130 140 150
FPIGRLDKDS QGLIFLTNHG DLVNKILRAG NDHEKEYLVT VDKPITEEFI
160 170 180 190 200
RGMSAGVPIL GTVTKKCKVK KEAPFVFRIT LVQGLNRQIR RMCEHFGYEV
210 220 230 240 250
KKLERTRIMN VSLSGIPLGE WRDLTDDELI DLFKLIENSS SEVKPKAKAK
260 270 280 290
PKTAGIKRPV VKMEKTAEKG GRPASNGKRF TSPGRKKKGR
Length:290
Mass (Da):32,477
Last modified:October 1, 1993 - v1
Checksum:iC88C830CBE5DF2FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43116.1.
U00096 Genomic DNA. Translation: AAC76992.1.
AP009048 Genomic DNA. Translation: BAE78024.1.
PIRiE65209.
RefSeqiNP_418446.1. NC_000913.3.
WP_000936377.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76992; AAC76992; b4022.
BAE78024; BAE78024; BAE78024.
GeneIDi948519.
KEGGiecj:JW3982.
eco:b4022.
PATRICi32123573. VBIEscCol129921_4135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43116.1.
U00096 Genomic DNA. Translation: AAC76992.1.
AP009048 Genomic DNA. Translation: BAE78024.1.
PIRiE65209.
RefSeqiNP_418446.1. NC_000913.3.
WP_000936377.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GMLX-ray2.60A/B66-290[»]
3DH3X-ray3.00A/B/C/D1-290[»]
ProteinModelPortaliP32684.
SMRiP32684. Positions 3-243.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261777. 6 interactions.
IntActiP32684. 9 interactions.
STRINGi511145.b4022.

Proteomic databases

PaxDbiP32684.
PRIDEiP32684.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76992; AAC76992; b4022.
BAE78024; BAE78024; BAE78024.
GeneIDi948519.
KEGGiecj:JW3982.
eco:b4022.
PATRICi32123573. VBIEscCol129921_4135.

Organism-specific databases

EchoBASEiEB1865.
EcoGeneiEG11921. rluF.

Phylogenomic databases

eggNOGiENOG4105FE9. Bacteria.
COG1187. LUCA.
HOGENOMiHOG000044954.
InParanoidiP32684.
KOiK06182.
OMAiCEYLGYE.
PhylomeDBiP32684.

Enzyme and pathway databases

BioCyciEcoCyc:EG11921-MONOMER.
ECOL316407:JW3982-MONOMER.
MetaCyc:EG11921-MONOMER.
BRENDAi5.4.99.21. 2026.

Miscellaneous databases

EvolutionaryTraceiP32684.
PROiP32684.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR000748. PsdUridine_synth_RsuA/RluB/E/F.
IPR018496. PsdUridine_synth_RsuA/RluB_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
IPR002942. S4_RNA-bd.
[Graphical view]
PfamiPF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]
SMARTiSM00363. S4. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00093. TIGR00093. 1 hit.
PROSITEiPS01149. PSI_RSU. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRLUF_ECOLI
AccessioniPrimary (citable) accession number: P32684
Secondary accession number(s): Q2M6T2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 7, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.