Ribosomal large subunit pseudouridine synthase F

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Reviewed, UniProtKB/Swiss-Prot P32684 (RLUF_ECOLI)

Last modified June 16, 2009. Version 74. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Ribosomal large subunit pseudouridine synthase F
    EC=5.4.99.-
Alternative name(s):
    rRNA-uridine isomerase F
    rRNA pseudouridylate synthase F

Gene names

Name:	rluF
Synonyms:	yjbC
Ordered Locus Names:	b4022, JW3982

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function

Responsible for synthesis of pseudouridine from uracil-2604 in 23S ribosomal RNA. Can, to a small extent, also react with uracil-2605. Ref.4

Catalytic activity

rRNA uridine = rRNA pseudouridine.

Sequence similarities

Belongs to the pseudouridine synthase rsuA family.

Contains 1 S4 RNA-binding domain.

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Ontologies

Keywords
Biological process	rRNA processing
Ligand	RNA-binding
Molecular function	Isomerase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW pseudouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 290

290

Ribosomal large subunit pseudouridine synthase F

PRO_0000100016

Regions

Domain

7 – 72

S4 RNA-binding

Sites

Active site

107

Experimental info

Mutagenesis

107

D → N or T: Loss of activity. Ref.4

Secondary structure

290

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P32684-1 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: C88C830CBE5DF2FA
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FASTA

290

32,477

        10         20         30         40         50         60 
MLPDSSVRLN KYISESGICS RREADRYIEQ GNVFLNGKRA TIGDQVKPGD VVKVNGQLIE 

        70         80         90        100        110        120 
PREAEDLVLI ALNKPVGIVS TTEDGERDNI VDFVNHSKRV FPIGRLDKDS QGLIFLTNHG 

       130        140        150        160        170        180 
DLVNKILRAG NDHEKEYLVT VDKPITEEFI RGMSAGVPIL GTVTKKCKVK KEAPFVFRIT 

       190        200        210        220        230        240 
LVQGLNRQIR RMCEHFGYEV KKLERTRIMN VSLSGIPLGE WRDLTDDELI DLFKLIENSS 

       250        260        270        280        290 
SEVKPKAKAK PKTAGIKRPV VKMEKTAEKG GRPASNGKRF TSPGRKKKGR

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Identification and site of action of the remaining four putative pseudouridine synthases in Escherichia coli." Del Campo M., Kaya Y., Ofengand J. RNA 7:1603-1615(2001) [PubMed: 11720289] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ASP-107. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

U00006 Genomic DNA. Translation: AAC43116.1.
U00096 Genomic DNA. Translation: AAC76992.1.
AP009048 Genomic DNA. Translation: BAE78024.1.

PIR

E65209.

RefSeq

AP_004523.1.
NP_418446.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GML	X-ray	2.60	A/B	66-290	[»]
3DH3	X-ray	3.00	A/B/C/D	1-290	[»]

ModBase

Search...

Genome annotation databases

GeneID

948519.

GenomeReviews

Gene locus JW3982 in contig AP009048_GR.
Gene locus b4022 in contig U00096_GR.

KEGG

ecj:JW3982.
eco:b4022.

Organism-specific databases

EchoBASE

EB1865.

EcoGene

EG11921. rluF.

CMR

Search...

Phylogenomic databases

HOGENOM

P32684.

OMA

P32684. MNVSLKG.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11921-MON.

Family and domain databases

InterPro

IPR006145. Pseudouridine_synth.
IPR000748. rRNA_pseudouridine_synth.
IPR018496. rRNA_pseudouridine_synth_CS.
IPR002942. S4_RNA_bd.
[Graphical view]

Pfam

PF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
[Graphical view]

SMART

SM00363. S4. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00093. Psi__synth_RSU. 1 hit.

PROSITE

PS01149. PSI_RSU. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

RLUF_ECOLI

Accession

Primary (citable) accession number: P32684
Secondary accession number(s): Q2M6T2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families