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Protein

23S rRNA pseudouridine(2604) synthase

Gene

rluF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of pseudouridine from uracil-2604 in 23S ribosomal RNA. Can, to a small extent, also react with uracil-2605.1 Publication

Catalytic activityi

23S rRNA uridine(2604) = 23S rRNA pseudouridine(2604).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei107Nucleophile1 Publication1

GO - Molecular functioni

  • pseudouridine synthase activity Source: EcoCyc
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • enzyme-directed rRNA pseudouridine synthesis Source: EcoCyc
  • tRNA pseudouridine synthesis Source: EcoCyc

Keywordsi

Molecular functionIsomerase, RNA-binding
Biological processrRNA processing

Enzyme and pathway databases

BioCyciEcoCyc:EG11921-MONOMER.
MetaCyc:EG11921-MONOMER.
BRENDAi5.4.99.21. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
23S rRNA pseudouridine(2604) synthase1 Publication (EC:5.4.99.212 Publications)
Alternative name(s):
Ribosomal large subunit pseudouridine synthase F
rRNA pseudouridylate synthase F
rRNA-uridine isomerase F
Gene namesi
Name:rluF
Synonyms:yjbC
Ordered Locus Names:b4022, JW3982
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11921. rluF.

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi107D → N or T: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001000161 – 29023S rRNA pseudouridine(2604) synthaseAdd BLAST290

Proteomic databases

PaxDbiP32684.
PRIDEiP32684.

Interactioni

Subunit structurei

Monomer.2 Publications

Protein-protein interaction databases

BioGridi4261777. 60 interactors.
IntActiP32684. 9 interactors.
STRINGi316385.ECDH10B_4211.

Structurei

Secondary structure

1290
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 14Combined sources6
Turni15 – 17Combined sources3
Helixi21 – 29Combined sources9
Beta strandi33 – 35Combined sources3
Beta strandi52 – 54Combined sources3
Beta strandi57 – 59Combined sources3
Helixi64 – 66Combined sources3
Beta strandi69 – 74Combined sources6
Beta strandi82 – 85Combined sources4
Beta strandi87 – 89Combined sources3
Helixi90 – 93Combined sources4
Beta strandi101 – 104Combined sources4
Beta strandi111 – 118Combined sources8
Helixi120 – 132Combined sources13
Beta strandi135 – 143Combined sources9
Helixi147 – 153Combined sources7
Beta strandi160 – 163Combined sources4
Beta strandi168 – 173Combined sources6
Beta strandi176 – 181Combined sources6
Helixi188 – 195Combined sources8
Beta strandi200 – 208Combined sources9
Beta strandi221 – 223Combined sources3
Helixi226 – 233Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GMLX-ray2.60A/B66-290[»]
3DH3X-ray3.00A/B/C/D1-290[»]
ProteinModelPortaliP32684.
SMRiP32684.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32684.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 72S4 RNA-bindingPROSITE-ProRule annotationAdd BLAST66

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni105 – 108Interaction with RNA1 Publication4
Regioni187 – 190Interaction with RNA1 Publication4

Sequence similaritiesi

Belongs to the pseudouridine synthase RsuA family.Curated

Phylogenomic databases

eggNOGiENOG4105FE9. Bacteria.
COG1187. LUCA.
HOGENOMiHOG000044954.
InParanoidiP32684.
KOiK06182.
PhylomeDBiP32684.

Family and domain databases

Gene3Di3.10.290.10. 1 hit.
InterProiView protein in InterPro
IPR020103. PsdUridine_synth_cat_dom.
IPR000748. PsdUridine_synth_RsuA/RluB/E/F.
IPR018496. PsdUridine_synth_RsuA/RluB_CS.
IPR006145. PsdUridine_synth_RsuA/RluD.
IPR002942. S4_RNA-bd.
PANTHERiPTHR21600. PTHR21600. 1 hit.
PfamiView protein in Pfam
PF00849. PseudoU_synth_2. 1 hit.
PF01479. S4. 1 hit.
SMARTiView protein in SMART
SM00363. S4. 1 hit.
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00093. TIGR00093. 1 hit.
PROSITEiView protein in PROSITE
PS01149. PSI_RSU. 1 hit.
PS50889. S4. 1 hit.

Sequencei

Sequence statusi: Complete.

P32684-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPDSSVRLN KYISESGICS RREADRYIEQ GNVFLNGKRA TIGDQVKPGD
60 70 80 90 100
VVKVNGQLIE PREAEDLVLI ALNKPVGIVS TTEDGERDNI VDFVNHSKRV
110 120 130 140 150
FPIGRLDKDS QGLIFLTNHG DLVNKILRAG NDHEKEYLVT VDKPITEEFI
160 170 180 190 200
RGMSAGVPIL GTVTKKCKVK KEAPFVFRIT LVQGLNRQIR RMCEHFGYEV
210 220 230 240 250
KKLERTRIMN VSLSGIPLGE WRDLTDDELI DLFKLIENSS SEVKPKAKAK
260 270 280 290
PKTAGIKRPV VKMEKTAEKG GRPASNGKRF TSPGRKKKGR
Length:290
Mass (Da):32,477
Last modified:October 1, 1993 - v1
Checksum:iC88C830CBE5DF2FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43116.1.
U00096 Genomic DNA. Translation: AAC76992.1.
AP009048 Genomic DNA. Translation: BAE78024.1.
PIRiE65209.
RefSeqiNP_418446.1. NC_000913.3.
WP_000936377.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76992; AAC76992; b4022.
BAE78024; BAE78024; BAE78024.
GeneIDi948519.
KEGGiecj:JW3982.
eco:b4022.
PATRICifig|1411691.4.peg.2691.

Similar proteinsi

Entry informationi

Entry nameiRLUF_ECOLI
AccessioniPrimary (citable) accession number: P32684
Secondary accession number(s): Q2M6T2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: August 30, 2017
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families