Fructose-like phosphotransferase enzyme IIB component 3

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P32676 (PTFB3_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 92. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Fructose-like phosphotransferase enzyme IIB component 3
EC=2.7.1.69

Alternative name(s):
PTS system fructose-like EIIB component 3

Gene names

Name:	frwD
Synonyms:	yijN
Ordered Locus Names:	b3953, JW3925

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	113 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cytoplasm Potential.
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 113

113

Fructose-like phosphotransferase enzyme IIB component 3

PRO_0000186505

Regions

Domain

1 – 100

100

PTS EIIB type-2

Sites

Active site

Phosphocysteine intermediate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P32676 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 36252AB21DDB09EC
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FASTA

113

12,637

        10         20         30         40         50         60 
MAYLVAVTAC VSGVAHTYMA AERLEKLCLL EKWGVSIETQ GALGTENRLA DEDIRRADVA 

        70         80         90        100        110 
LLITDIELAG AERFEHCRYV QCSIYAFLRE PQRVMSAVRK VLSAPQQTHL ILE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Novel phosphotransferase system genes revealed by bacterial genome analysis -- a gene cluster encoding a unique Enzyme I and the proteins of a fructose-like permease system." Reizer J., Reizer A., Saier M.H. Jr. Microbiology 141:961-971(1995) [PubMed: 7773398] [Abstract] Cited for: DISCUSSION OF SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00006 Genomic DNA. Translation: AAC43059.1. U00096 Genomic DNA. Translation: AAC76935.1. AP009048 Genomic DNA. Translation: BAE77358.1.
PIR	D65202.
RefSeq	NP_418388.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P32676.
SMR	P32676. Positions 2-100.
ModBase	Search...
Protein family/group databases
TCDB	4.A.2.1.10. PTS fructose-mannitol (Fru) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003911; EBESCP00000003911; EBESCG00000003198. EBESCT00000016492; EBESCP00000015783; EBESCG00000015552.
GeneID	948452.
GenomeReviews	Gene locus JW3925 in contig AP009048_GR. Gene locus b3953 in contig U00096_GR.
KEGG	ecj:JW3925. eco:b3953.
PATRIC	32123427. VBIEscCol129921_4074.
Organism-specific databases
EchoBASE	EB1856.
EcoGene	EG11912. frwD.
Phylogenomic databases
eggNOG	COG1445.
GeneTree	EBGT00070000031719.
HOGENOM	HBG564028.
OMA	CQQEKWN.
PhylomeDB	P32676.
ProtClustDB	PRK10427.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11912-MONOMER.
Gene expression databases
Genevestigator	P32676.
Family and domain databases
InterPro	IPR013011. PTS_EIIB_2. IPR003501. PTS_EIIB_2/3. IPR003353. PTS_IIB_fruc. [Graphical view]
KO	K11202.
Pfam	PF02302. PTS_IIB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00829. FRU. 1 hit.
PROSITE	PS51099. PTS_EIIB_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTFB3_ECOLI

Accession

Primary (citable) accession number: P32676
Secondary accession number(s): Q2M8P8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	January 25, 2012
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents