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Protein

PTS system fructose-like EIIB component 3

Gene

frwD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane.By similarity1 Publication

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-fructose(Side 1) = [protein]-L-histidine + D-fructose 1-phosphate(Side 2).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei10Phosphocysteine intermediateBy similarityCurated1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11912-MONOMER.
ECOL316407:JW3925-MONOMER.

Protein family/group databases

TCDBi4.A.2.1.10. the pts fructose-mannitol (fru) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system fructose-like EIIB component 3By similarity (EC:2.7.1.202By similarity)
Alternative name(s):
Fructose-like phosphotransferase enzyme IIB component 3By similarity
Gene namesi
Name:frwD
Synonyms:yijN
Ordered Locus Names:b3953, JW3925
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11912. frwD.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001865051 – 113PTS system fructose-like EIIB component 3Add BLAST113

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10Phosphocysteine; by EIIAPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP32676.
PRIDEiP32676.

Interactioni

Protein-protein interaction databases

BioGridi4263010. 6 interactors.
IntActiP32676. 2 interactors.
STRINGi511145.b3953.

Structurei

Secondary structure

1113
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 9Combined sources7
Turni13 – 17Combined sources5
Helixi18 – 30Combined sources13
Beta strandi34 – 41Combined sources8
Beta strandi44 – 47Combined sources4
Helixi51 – 56Combined sources6
Beta strandi58 – 66Combined sources9
Helixi71 – 74Combined sources4
Beta strandi79 – 82Combined sources4
Helixi84 – 89Combined sources6
Helixi92 – 103Combined sources12
Beta strandi104 – 106Combined sources3
Beta strandi108 – 111Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4TN5X-ray2.29A/B1-113[»]
ProteinModelPortaliP32676.
SMRiP32676.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 100PTS EIIB type-2PROSITE-ProRule annotationAdd BLAST100

Domaini

The PTS EIIB type-2 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-2 domain.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 PTS EIIB type-2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108XCZ. Bacteria.
COG1445. LUCA.
HOGENOMiHOG000231313.
KOiK11202.
OMAiCQQEKWN.
PhylomeDBiP32676.

Family and domain databases

CDDicd05569. PTS_IIB_fructose. 1 hit.
InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003353. PTS_IIB_fruc.
[Graphical view]
PfamiPF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00829. FRU. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32676-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYLVAVTAC VSGVAHTYMA AERLEKLCLL EKWGVSIETQ GALGTENRLA
60 70 80 90 100
DEDIRRADVA LLITDIELAG AERFEHCRYV QCSIYAFLRE PQRVMSAVRK
110
VLSAPQQTHL ILE
Length:113
Mass (Da):12,637
Last modified:October 1, 1993 - v1
Checksum:i36252AB21DDB09EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43059.1.
U00096 Genomic DNA. Translation: AAC76935.1.
AP009048 Genomic DNA. Translation: BAE77358.1.
PIRiD65202.
RefSeqiNP_418388.1. NC_000913.3.
WP_000323846.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76935; AAC76935; b3953.
BAE77358; BAE77358; BAE77358.
GeneIDi948452.
KEGGiecj:JW3925.
eco:b3953.
PATRICi32123427. VBIEscCol129921_4074.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43059.1.
U00096 Genomic DNA. Translation: AAC76935.1.
AP009048 Genomic DNA. Translation: BAE77358.1.
PIRiD65202.
RefSeqiNP_418388.1. NC_000913.3.
WP_000323846.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4TN5X-ray2.29A/B1-113[»]
ProteinModelPortaliP32676.
SMRiP32676.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263010. 6 interactors.
IntActiP32676. 2 interactors.
STRINGi511145.b3953.

Protein family/group databases

TCDBi4.A.2.1.10. the pts fructose-mannitol (fru) family.

Proteomic databases

PaxDbiP32676.
PRIDEiP32676.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76935; AAC76935; b3953.
BAE77358; BAE77358; BAE77358.
GeneIDi948452.
KEGGiecj:JW3925.
eco:b3953.
PATRICi32123427. VBIEscCol129921_4074.

Organism-specific databases

EchoBASEiEB1856.
EcoGeneiEG11912. frwD.

Phylogenomic databases

eggNOGiENOG4108XCZ. Bacteria.
COG1445. LUCA.
HOGENOMiHOG000231313.
KOiK11202.
OMAiCQQEKWN.
PhylomeDBiP32676.

Enzyme and pathway databases

BioCyciEcoCyc:EG11912-MONOMER.
ECOL316407:JW3925-MONOMER.

Miscellaneous databases

PROiP32676.

Family and domain databases

CDDicd05569. PTS_IIB_fructose. 1 hit.
InterProiIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003353. PTS_IIB_fruc.
[Graphical view]
PfamiPF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00829. FRU. 1 hit.
PROSITEiPS51099. PTS_EIIB_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTFB3_ECOLI
AccessioniPrimary (citable) accession number: P32676
Secondary accession number(s): Q2M8P8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.