Reviewed, UniProtKB/Swiss-Prot P32676 (PTFB3_ECOLI)

Names and origin

Protein names

Recommended name:
    Fructose-like phosphotransferase enzyme IIB component 3
    EC=2.7.1.69
Alternative name(s):
    PTS system fructose-like EIIB component 3

Gene names

Name:	frwD
Synonyms:	yijN
Ordered Locus Names:	b3953, JW3925

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	113 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cytoplasm Potential.
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 113	113	Fructose-like phosphotransferase enzyme IIB component 3		PRO_0000186505
Regions
	Domain	1 – 100	100	PTS EIIB type-2
Sites
	Active site	10	1	Phosphocysteine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32676-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 36252AB21DDB09EC	FASTA	113	12,637
	10 20 30 40 50 60 MAYLVAVTAC VSGVAHTYMA AERLEKLCLL EKWGVSIETQ GALGTENRLA DEDIRRADVA 70 80 90 100 110 LLITDIELAG AERFEHCRYV QCSIYAFLRE PQRVMSAVRK VLSAPQQTHL ILE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Novel phosphotransferase system genes revealed by bacterial genome analysis -- a gene cluster encoding a unique Enzyme I and the proteins of a fructose-like permease system." Reizer J., Reizer A., Saier M.H. Jr. Microbiology 141:961-971(1995) [PubMed: 7773398] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00006 Genomic DNA. Translation: AAC43059.1. U00096 Genomic DNA. Translation: AAC76935.1. AP009048 Genomic DNA. Translation: BAE77358.1.
PIR	D65202.
RefSeq	AP_003857.1. NP_418388.1.
3D structure databases
SMR	P32676. Positions 3-97.
ModBase	Search...
Protein-protein interaction databases
STRING	P32676.
Protein family/group databases
TCDB	4.A.2.1.10. PTS fructose-mannitol (Fru) family.
Genome annotation databases
GeneID	948452.
GenomeReviews	Gene locus JW3925 in contig AP009048_GR. Gene locus b3953 in contig U00096_GR.
KEGG	ecj:JW3925. eco:b3953.
Organism-specific databases
EchoBASE	EB1856.
EcoGene	EG11912. frwD.
CMR	Search...
Phylogenomic databases
eggNOG	COG1445.
HOGENOM	HBG564028.
OMA	PQQVMSA.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11912-MONOMER. ECOL168927:B3953-MONOMER.
Gene expression databases
Genevestigator	P32676.
Family and domain databases
InterPro	IPR013011. PTS_EIIB_2. IPR003501. PTS_EIIB_2/3. IPR003353. PTS_IIB_fruc. [Graphical view]
Pfam	PF02302. PTS_IIB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00829. FRU. 1 hit.
PROSITE	PS51099. PTS_EIIB_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTFB3_ECOLI

Accession

Primary (citable) accession number: P32676
Secondary accession number(s): Q2M8P8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	February 9, 2010
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)