Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P32675

- PFLC_ECOLI

UniProt

P32675 - PFLC_ECOLI

Protein

Pyruvate formate-lyase 2-activating enzyme

Gene

pflC

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 2 (01 Oct 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi51 – 511Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi54 – 541Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

    GO - Molecular functioni

    1. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glucose metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11911-MONOMER.
    ECOL316407:JW3924-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate formate-lyase 2-activating enzyme (EC:1.97.1.4)
    Alternative name(s):
    Formate-C-acetyltransferase-activating enzyme 2
    PFL-activating enzyme 2
    Gene namesi
    Name:pflC
    Synonyms:yijM
    Ordered Locus Names:b3952, JW3924
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11911. pflC.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 292292Pyruvate formate-lyase 2-activating enzymePRO_0000200527Add
    BLAST

    Proteomic databases

    PaxDbiP32675.
    PRIDEiP32675.

    Expressioni

    Gene expression databases

    GenevestigatoriP32675.

    Interactioni

    Protein-protein interaction databases

    IntActiP32675. 3 interactions.
    STRINGi511145.b3952.

    Structurei

    3D structure databases

    ProteinModelPortaliP32675.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini62 – 96354Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1180.
    HOGENOMiHOG000011459.
    KOiK04069.
    OMAiHRCPWCA.
    OrthoDBiEOG63VBWZ.
    PhylomeDBiP32675.

    Family and domain databases

    InterProiIPR017896. 4Fe4S_Fe-S-bd.
    IPR006638. Elp3/MiaB/NifB.
    IPR012839. Glycyl_radical_activase.
    IPR001989. Radical_activat_CS.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000371. PFL_act_enz. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    PROSITEiPS51379. 4FE4S_FER_2. 1 hit.
    PS01087. RADICAL_ACTIVATING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32675-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSSAGQRIS CNVVETRRDD VARIFNIQRY SLNDGEGIRT VVFFKGCPHL    50
    CPWCANPESI SGKIQTVRRE AKCLHCAKCL RDADECPSGA FERIGRDISL 100
    DALEREVMKD DIFFRTSGGG VTLSGGEVLM QAEFATRFLQ RLRLWGVSCA 150
    IETAGDAPAS KLLPLAKLCD EVLFDLKIMD ATQARDVVKM NLPRVLENLR 200
    LLVSEGVNVI PRLPLIPGFT LSRENMQQAL DVLIPLNIRQ IHLLPFHQYG 250
    EPKYRLLGKT WSMKEVPAPS SADVATMREM AERAGLQVTV GG 292
    Length:292
    Mass (Da):32,430
    Last modified:October 1, 1994 - v2
    Checksum:i4842A78940E84A7B
    GO

    Sequence cautioni

    The sequence AAC43058.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00006 Genomic DNA. Translation: AAC43058.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC76934.1.
    AP009048 Genomic DNA. Translation: BAE77359.1.
    PIRiC65202.
    RefSeqiNP_418387.3. NC_000913.3.
    YP_491500.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76934; AAC76934; b3952.
    BAE77359; BAE77359; BAE77359.
    GeneIDi12934017.
    948453.
    KEGGiecj:Y75_p3236.
    eco:b3952.
    PATRICi32123425. VBIEscCol129921_4073.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00006 Genomic DNA. Translation: AAC43058.1 . Different initiation.
    U00096 Genomic DNA. Translation: AAC76934.1 .
    AP009048 Genomic DNA. Translation: BAE77359.1 .
    PIRi C65202.
    RefSeqi NP_418387.3. NC_000913.3.
    YP_491500.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P32675.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P32675. 3 interactions.
    STRINGi 511145.b3952.

    Proteomic databases

    PaxDbi P32675.
    PRIDEi P32675.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76934 ; AAC76934 ; b3952 .
    BAE77359 ; BAE77359 ; BAE77359 .
    GeneIDi 12934017.
    948453.
    KEGGi ecj:Y75_p3236.
    eco:b3952.
    PATRICi 32123425. VBIEscCol129921_4073.

    Organism-specific databases

    EchoBASEi EB1855.
    EcoGenei EG11911. pflC.

    Phylogenomic databases

    eggNOGi COG1180.
    HOGENOMi HOG000011459.
    KOi K04069.
    OMAi HRCPWCA.
    OrthoDBi EOG63VBWZ.
    PhylomeDBi P32675.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG11911-MONOMER.
    ECOL316407:JW3924-MONOMER.

    Miscellaneous databases

    PROi P32675.

    Gene expression databases

    Genevestigatori P32675.

    Family and domain databases

    InterProi IPR017896. 4Fe4S_Fe-S-bd.
    IPR006638. Elp3/MiaB/NifB.
    IPR012839. Glycyl_radical_activase.
    IPR001989. Radical_activat_CS.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000371. PFL_act_enz. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    PROSITEi PS51379. 4FE4S_FER_2. 1 hit.
    PS01087. RADICAL_ACTIVATING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
      Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
      Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Novel phosphotransferase system genes revealed by bacterial genome analysis -- a gene cluster encoding a unique Enzyme I and the proteins of a fructose-like permease system."
      Reizer J., Reizer A., Saier M.H. Jr.
      Microbiology 141:961-971(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISCUSSION OF SEQUENCE.

    Entry informationi

    Entry nameiPFLC_ECOLI
    AccessioniPrimary (citable) accession number: P32675
    Secondary accession number(s): Q2M8P7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3