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P32675 (PFLC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate formate-lyase 2-activating enzyme

EC=1.97.1.4
Alternative name(s):
Formate-C-acetyltransferase-activating enzyme 2
PFL-activating enzyme 2
Gene names
Name:pflC
Synonyms:yijM
Ordered Locus Names:b3952, JW3924
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length292 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity.

Catalytic activity

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the organic radical-activating enzymes family.

Contains 1 4Fe-4S ferredoxin-type domain.

Sequence caution

The sequence AAC43058.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 292292Pyruvate formate-lyase 2-activating enzyme
PRO_0000200527

Regions

Domain62 – 96354Fe-4S ferredoxin-type

Sites

Metal binding471Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding511Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding541Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
P32675 [UniParc].

Last modified October 1, 1994. Version 2.
Checksum: 4842A78940E84A7B

FASTA29232,430
        10         20         30         40         50         60 
MTSSAGQRIS CNVVETRRDD VARIFNIQRY SLNDGEGIRT VVFFKGCPHL CPWCANPESI 

        70         80         90        100        110        120 
SGKIQTVRRE AKCLHCAKCL RDADECPSGA FERIGRDISL DALEREVMKD DIFFRTSGGG 

       130        140        150        160        170        180 
VTLSGGEVLM QAEFATRFLQ RLRLWGVSCA IETAGDAPAS KLLPLAKLCD EVLFDLKIMD 

       190        200        210        220        230        240 
ATQARDVVKM NLPRVLENLR LLVSEGVNVI PRLPLIPGFT LSRENMQQAL DVLIPLNIRQ 

       250        260        270        280        290 
IHLLPFHQYG EPKYRLLGKT WSMKEVPAPS SADVATMREM AERAGLQVTV GG 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Novel phosphotransferase system genes revealed by bacterial genome analysis -- a gene cluster encoding a unique Enzyme I and the proteins of a fructose-like permease system."
Reizer J., Reizer A., Saier M.H. Jr.
Microbiology 141:961-971(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00006 Genomic DNA. Translation: AAC43058.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76934.1.
AP009048 Genomic DNA. Translation: BAE77359.1.
PIRC65202.
RefSeqNP_418387.3. NC_000913.3.
YP_491500.1. NC_007779.1.

3D structure databases

ProteinModelPortalP32675.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP32675. 3 interactions.
STRING511145.b3952.

Proteomic databases

PaxDbP32675.
PRIDEP32675.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76934; AAC76934; b3952.
BAE77359; BAE77359; BAE77359.
GeneID12934017.
948453.
KEGGecj:Y75_p3236.
eco:b3952.
PATRIC32123425. VBIEscCol129921_4073.

Organism-specific databases

EchoBASEEB1855.
EcoGeneEG11911. pflC.

Phylogenomic databases

eggNOGCOG1180.
HOGENOMHOG000011459.
KOK04069.
OMAHRCPWCA.
OrthoDBEOG63VBWZ.
PhylomeDBP32675.

Enzyme and pathway databases

BioCycEcoCyc:EG11911-MONOMER.
ECOL316407:JW3924-MONOMER.

Gene expression databases

GenevestigatorP32675.

Family and domain databases

InterProIPR017896. 4Fe4S_Fe-S-bd.
IPR006638. Elp3/MiaB/NifB.
IPR012839. Glycyl_radical_activase.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF000371. PFL_act_enz. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
PROSITEPS51379. 4FE4S_FER_2. 1 hit.
PS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP32675.

Entry information

Entry namePFLC_ECOLI
AccessionPrimary (citable) accession number: P32675
Secondary accession number(s): Q2M8P7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1994
Last modified: June 11, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene