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Protein

Pyruvate formate-lyase 2-activating enzyme

Gene

pflC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi51 – 511Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi54 – 541Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG11911-MONOMER.
ECOL316407:JW3924-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase 2-activating enzyme (EC:1.97.1.4)
Alternative name(s):
Formate-C-acetyltransferase-activating enzyme 2
PFL-activating enzyme 2
Gene namesi
Name:pflC
Synonyms:yijM
Ordered Locus Names:b3952, JW3924
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11911. pflC.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 292292Pyruvate formate-lyase 2-activating enzymePRO_0000200527Add
BLAST

Proteomic databases

PaxDbiP32675.
PRIDEiP32675.

Interactioni

Protein-protein interaction databases

IntActiP32675. 3 interactions.
STRINGi511145.b3952.

Structurei

3D structure databases

ProteinModelPortaliP32675.
SMRiP32675. Positions 21-288.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini62 – 96354Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1180.
HOGENOMiHOG000011459.
InParanoidiP32675.
KOiK04069.
OMAiHRCPWCA.
OrthoDBiEOG63VBWZ.
PhylomeDBiP32675.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR006638. Elp3/MiaB/NifB.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
PROSITEiPS51379. 4FE4S_FER_2. 1 hit.
PS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32675-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSSAGQRIS CNVVETRRDD VARIFNIQRY SLNDGEGIRT VVFFKGCPHL
60 70 80 90 100
CPWCANPESI SGKIQTVRRE AKCLHCAKCL RDADECPSGA FERIGRDISL
110 120 130 140 150
DALEREVMKD DIFFRTSGGG VTLSGGEVLM QAEFATRFLQ RLRLWGVSCA
160 170 180 190 200
IETAGDAPAS KLLPLAKLCD EVLFDLKIMD ATQARDVVKM NLPRVLENLR
210 220 230 240 250
LLVSEGVNVI PRLPLIPGFT LSRENMQQAL DVLIPLNIRQ IHLLPFHQYG
260 270 280 290
EPKYRLLGKT WSMKEVPAPS SADVATMREM AERAGLQVTV GG
Length:292
Mass (Da):32,430
Last modified:October 1, 1994 - v2
Checksum:i4842A78940E84A7B
GO

Sequence cautioni

The sequence AAC43058.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43058.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76934.1.
AP009048 Genomic DNA. Translation: BAE77359.1.
PIRiC65202.
RefSeqiNP_418387.3. NC_000913.3.
WP_000204105.1. NZ_CP010445.1.

Genome annotation databases

EnsemblBacteriaiAAC76934; AAC76934; b3952.
BAE77359; BAE77359; BAE77359.
GeneIDi948453.
KEGGieco:b3952.
PATRICi32123425. VBIEscCol129921_4073.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43058.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76934.1.
AP009048 Genomic DNA. Translation: BAE77359.1.
PIRiC65202.
RefSeqiNP_418387.3. NC_000913.3.
WP_000204105.1. NZ_CP010445.1.

3D structure databases

ProteinModelPortaliP32675.
SMRiP32675. Positions 21-288.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP32675. 3 interactions.
STRINGi511145.b3952.

Proteomic databases

PaxDbiP32675.
PRIDEiP32675.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76934; AAC76934; b3952.
BAE77359; BAE77359; BAE77359.
GeneIDi948453.
KEGGieco:b3952.
PATRICi32123425. VBIEscCol129921_4073.

Organism-specific databases

EchoBASEiEB1855.
EcoGeneiEG11911. pflC.

Phylogenomic databases

eggNOGiCOG1180.
HOGENOMiHOG000011459.
InParanoidiP32675.
KOiK04069.
OMAiHRCPWCA.
OrthoDBiEOG63VBWZ.
PhylomeDBiP32675.

Enzyme and pathway databases

BioCyciEcoCyc:EG11911-MONOMER.
ECOL316407:JW3924-MONOMER.

Miscellaneous databases

PROiP32675.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR006638. Elp3/MiaB/NifB.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
PROSITEiPS51379. 4FE4S_FER_2. 1 hit.
PS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
    Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
    Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Novel phosphotransferase system genes revealed by bacterial genome analysis -- a gene cluster encoding a unique Enzyme I and the proteins of a fructose-like permease system."
    Reizer J., Reizer A., Saier M.H. Jr.
    Microbiology 141:961-971(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.

Entry informationi

Entry nameiPFLC_ECOLI
AccessioniPrimary (citable) accession number: P32675
Secondary accession number(s): Q2M8P7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1994
Last modified: July 22, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.