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P32675

- PFLC_ECOLI

UniProt

P32675 - PFLC_ECOLI

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Protein

Pyruvate formate-lyase 2-activating enzyme

Gene

pflC

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi51 – 511Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
Metal bindingi54 – 541Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

GO - Molecular functioni

  1. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glucose metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG11911-MONOMER.
ECOL316407:JW3924-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase 2-activating enzyme (EC:1.97.1.4)
Alternative name(s):
Formate-C-acetyltransferase-activating enzyme 2
PFL-activating enzyme 2
Gene namesi
Name:pflC
Synonyms:yijM
Ordered Locus Names:b3952, JW3924
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11911. pflC.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 292292Pyruvate formate-lyase 2-activating enzymePRO_0000200527Add
BLAST

Proteomic databases

PaxDbiP32675.
PRIDEiP32675.

Expressioni

Gene expression databases

GenevestigatoriP32675.

Interactioni

Protein-protein interaction databases

IntActiP32675. 3 interactions.
STRINGi511145.b3952.

Structurei

3D structure databases

ProteinModelPortaliP32675.
SMRiP32675. Positions 21-288.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini62 – 96354Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 4Fe-4S ferredoxin-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1180.
HOGENOMiHOG000011459.
InParanoidiP32675.
KOiK04069.
OMAiHRCPWCA.
OrthoDBiEOG63VBWZ.
PhylomeDBiP32675.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR006638. Elp3/MiaB/NifB.
IPR012839. Glycyl_radical_activase.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF000371. PFL_act_enz. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
PROSITEiPS51379. 4FE4S_FER_2. 1 hit.
PS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32675-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSSAGQRIS CNVVETRRDD VARIFNIQRY SLNDGEGIRT VVFFKGCPHL
60 70 80 90 100
CPWCANPESI SGKIQTVRRE AKCLHCAKCL RDADECPSGA FERIGRDISL
110 120 130 140 150
DALEREVMKD DIFFRTSGGG VTLSGGEVLM QAEFATRFLQ RLRLWGVSCA
160 170 180 190 200
IETAGDAPAS KLLPLAKLCD EVLFDLKIMD ATQARDVVKM NLPRVLENLR
210 220 230 240 250
LLVSEGVNVI PRLPLIPGFT LSRENMQQAL DVLIPLNIRQ IHLLPFHQYG
260 270 280 290
EPKYRLLGKT WSMKEVPAPS SADVATMREM AERAGLQVTV GG
Length:292
Mass (Da):32,430
Last modified:October 1, 1994 - v2
Checksum:i4842A78940E84A7B
GO

Sequence cautioni

The sequence AAC43058.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43058.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76934.1.
AP009048 Genomic DNA. Translation: BAE77359.1.
PIRiC65202.
RefSeqiNP_418387.3. NC_000913.3.
YP_491500.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76934; AAC76934; b3952.
BAE77359; BAE77359; BAE77359.
GeneIDi12934017.
948453.
KEGGiecj:Y75_p3236.
eco:b3952.
PATRICi32123425. VBIEscCol129921_4073.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43058.1 . Different initiation.
U00096 Genomic DNA. Translation: AAC76934.1 .
AP009048 Genomic DNA. Translation: BAE77359.1 .
PIRi C65202.
RefSeqi NP_418387.3. NC_000913.3.
YP_491500.1. NC_007779.1.

3D structure databases

ProteinModelPortali P32675.
SMRi P32675. Positions 21-288.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P32675. 3 interactions.
STRINGi 511145.b3952.

Proteomic databases

PaxDbi P32675.
PRIDEi P32675.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76934 ; AAC76934 ; b3952 .
BAE77359 ; BAE77359 ; BAE77359 .
GeneIDi 12934017.
948453.
KEGGi ecj:Y75_p3236.
eco:b3952.
PATRICi 32123425. VBIEscCol129921_4073.

Organism-specific databases

EchoBASEi EB1855.
EcoGenei EG11911. pflC.

Phylogenomic databases

eggNOGi COG1180.
HOGENOMi HOG000011459.
InParanoidi P32675.
KOi K04069.
OMAi HRCPWCA.
OrthoDBi EOG63VBWZ.
PhylomeDBi P32675.

Enzyme and pathway databases

BioCyci EcoCyc:EG11911-MONOMER.
ECOL316407:JW3924-MONOMER.

Miscellaneous databases

PROi P32675.

Gene expression databases

Genevestigatori P32675.

Family and domain databases

InterProi IPR017896. 4Fe4S_Fe-S-bd.
IPR006638. Elp3/MiaB/NifB.
IPR012839. Glycyl_radical_activase.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view ]
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF000371. PFL_act_enz. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
PROSITEi PS51379. 4FE4S_FER_2. 1 hit.
PS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
    Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
    Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Novel phosphotransferase system genes revealed by bacterial genome analysis -- a gene cluster encoding a unique Enzyme I and the proteins of a fructose-like permease system."
    Reizer J., Reizer A., Saier M.H. Jr.
    Microbiology 141:961-971(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.

Entry informationi

Entry nameiPFLC_ECOLI
AccessioniPrimary (citable) accession number: P32675
Secondary accession number(s): Q2M8P7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1994
Last modified: November 26, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3