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Protein

Pyruvate formate-lyase 2-activating enzyme

Gene

pflC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi51Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi54Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Binding sitei126S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei247S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG11911-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase 2-activating enzyme (EC:1.97.1.4)
Alternative name(s):
Formate-C-acetyltransferase-activating enzyme 2
PFL-activating enzyme 2
Gene namesi
Name:pflC
Synonyms:yijM
Ordered Locus Names:b3952, JW3924
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11911 pflC

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002005271 – 292Pyruvate formate-lyase 2-activating enzymeAdd BLAST292

Proteomic databases

PaxDbiP32675
PRIDEiP32675

Interactioni

Protein-protein interaction databases

IntActiP32675, 3 interactors
STRINGi316385.ECDH10B_4140

Structurei

3D structure databases

ProteinModelPortaliP32675
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini62 – 964Fe-4S ferredoxin-typePROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni53 – 55S-adenosyl-L-methionine bindingBy similarity3
Regioni175 – 177S-adenosyl-L-methionine bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108AHH Bacteria
COG1180 LUCA
HOGENOMiHOG000011459
InParanoidiP32675
KOiK04069
OMAiHRCPWCA
PhylomeDBiP32675

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR013785 Aldolase_TIM
IPR034457 Organic_radical-activating
IPR012839 Organic_radical_activase
IPR001989 Radical_activat_CS
IPR007197 rSAM
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF000371 PFL_act_enz, 1 hit
SFLDiSFLDG01066 organic_radical-activating_enz, 1 hit
SFLDS00029 Radical_SAM, 1 hit
PROSITEiView protein in PROSITE
PS51379 4FE4S_FER_2, 1 hit
PS01087 RADICAL_ACTIVATING, 1 hit

Sequencei

Sequence statusi: Complete.

P32675-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSSAGQRIS CNVVETRRDD VARIFNIQRY SLNDGEGIRT VVFFKGCPHL
60 70 80 90 100
CPWCANPESI SGKIQTVRRE AKCLHCAKCL RDADECPSGA FERIGRDISL
110 120 130 140 150
DALEREVMKD DIFFRTSGGG VTLSGGEVLM QAEFATRFLQ RLRLWGVSCA
160 170 180 190 200
IETAGDAPAS KLLPLAKLCD EVLFDLKIMD ATQARDVVKM NLPRVLENLR
210 220 230 240 250
LLVSEGVNVI PRLPLIPGFT LSRENMQQAL DVLIPLNIRQ IHLLPFHQYG
260 270 280 290
EPKYRLLGKT WSMKEVPAPS SADVATMREM AERAGLQVTV GG
Length:292
Mass (Da):32,430
Last modified:October 1, 1994 - v2
Checksum:i4842A78940E84A7B
GO

Sequence cautioni

The sequence AAC43058 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA Translation: AAC43058.1 Different initiation.
U00096 Genomic DNA Translation: AAC76934.1
AP009048 Genomic DNA Translation: BAE77359.1
PIRiC65202
RefSeqiNP_418387.3, NC_000913.3
WP_000204105.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76934; AAC76934; b3952
BAE77359; BAE77359; BAE77359
GeneIDi948453
KEGGiecj:JW3924
eco:b3952
PATRICifig|1411691.4.peg.2753

Similar proteinsi

Entry informationi

Entry nameiPFLC_ECOLI
AccessioniPrimary (citable) accession number: P32675
Secondary accession number(s): Q2M8P7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1994
Last modified: April 25, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health