P32675 (PFLC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate formate-lyase 2-activating enzyme
EC=1.97.1.4

Alternative name(s):
Formate-C-acetyltransferase-activating enzyme 2
PFL-activating enzyme 2

Gene names

Name:	pflC
Synonyms:	yijM
Ordered Locus Names:	b3952, JW3924

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity.
Catalytic activity	S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the organic radical-activating enzymes family. Contains 1 4Fe-4S ferredoxin-type domain.
Sequence caution	The sequence AAC43058.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW [formate-C-acetyltransferase]-activating enzyme activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Pyruvate formate-lyase 2-activating enzyme		PRO_0000200527
Regions
	Domain	62 – 96	35	4Fe-4S ferredoxin-type
Sites
	Metal binding	47	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	51	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	54	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P32675 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: 4842A78940E84A7B	FASTA	292	32,430
	10 20 30 40 50 60 MTSSAGQRIS CNVVETRRDD VARIFNIQRY SLNDGEGIRT VVFFKGCPHL CPWCANPESI 70 80 90 100 110 120 SGKIQTVRRE AKCLHCAKCL RDADECPSGA FERIGRDISL DALEREVMKD DIFFRTSGGG 130 140 150 160 170 180 VTLSGGEVLM QAEFATRFLQ RLRLWGVSCA IETAGDAPAS KLLPLAKLCD EVLFDLKIMD 190 200 210 220 230 240 ATQARDVVKM NLPRVLENLR LLVSEGVNVI PRLPLIPGFT LSRENMQQAL DVLIPLNIRQ 250 260 270 280 290 IHLLPFHQYG EPKYRLLGKT WSMKEVPAPS SADVATMREM AERAGLQVTV GG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Novel phosphotransferase system genes revealed by bacterial genome analysis -- a gene cluster encoding a unique Enzyme I and the proteins of a fructose-like permease system." Reizer J., Reizer A., Saier M.H. Jr. Microbiology 141:961-971(1995) [PubMed: 7773398] [Abstract] Cited for: DISCUSSION OF SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00006 Genomic DNA. Translation: AAC43058.1. Different initiation. U00096 Genomic DNA. Translation: AAC76934.1. AP009048 Genomic DNA. Translation: BAE77359.1.
PIR	C65202.
RefSeq	NP_418387.3. NC_000913.2.
3D structure databases
ProteinModelPortal	P32675.
SMR	P32675. Positions 20-292.
ModBase	Search...
Protein-protein interaction databases
IntAct	P32675. 3 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001950; EBESCP00000001950; EBESCG00000001599. EBESCT00000014274; EBESCP00000013565; EBESCG00000013336.
GeneID	948453.
GenomeReviews	Gene locus JW3924 in contig AP009048_GR. Gene locus b3952 in contig U00096_GR.
KEGG	ecj:JW3924. eco:b3952.
PATRIC	32123425. VBIEscCol129921_4073.
Organism-specific databases
EchoBASE	EB1855.
EcoGene	EG11911. pflC.
Phylogenomic databases
eggNOG	COG1180.
GeneTree	EBGT00050000009897.
HOGENOM	HBG554871.
OMA	LIPGYTL.
PhylomeDB	P32675.
ProtClustDB	PRK10076.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11911-MONOMER.
Gene expression databases
Genevestigator	P32675.
Family and domain databases
InterPro	IPR017896. 4Fe4S_Fe-S-bd. IPR006638. Elp3/MiaB/NifB. IPR012839. Glycyl_radical_activase. IPR001989. Radical_activat_CS. IPR007197. rSAM. [Graphical view]
KO	K04069.
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF000371. PFL_act_enz. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
PROSITE	PS51379. 4FE4S_FER_2. 1 hit. PS01087. RADICAL_ACTIVATING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PFLC_ECOLI

Accession

Primary (citable) accession number: P32675
Secondary accession number(s): Q2M8P7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1994
Last modified:	January 25, 2012
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program