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Protein

Formate acetyltransferase 2

Gene

pflD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Acetyl-CoA + formate = CoA + pyruvate.

Pathwayi: pyruvate fermentation

This protein is involved in step 1 of the subpathway that synthesizes formate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. Putative formate acetyltransferase 3 (ybiW), Formate acetyltransferase 1 (pflB), Formate acetyltransferase 2 (pflD)
This subpathway is part of the pathway pyruvate fermentation, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes formate from pyruvate, the pathway pyruvate fermentation and in Fermentation.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:EG11910-MONOMER
UniPathwayiUPA00920; UER00891

Names & Taxonomyi

Protein namesi
Recommended name:
Formate acetyltransferase 2 (EC:2.3.1.54)
Alternative name(s):
Pyruvate formate-lyase 2
Gene namesi
Name:pflD
Synonyms:yijL
Ordered Locus Names:b3951, JW3923
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11910 pflD

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001666891 – 765Formate acetyltransferase 2Add BLAST765

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei741Glycine radicalPROSITE-ProRule annotation1

Keywords - PTMi

Organic radical

Proteomic databases

PaxDbiP32674
PRIDEiP32674

Interactioni

Protein-protein interaction databases

BioGridi4262065, 3 interactors
DIPiDIP-10469N
IntActiP32674, 4 interactors
STRINGi316385.ECDH10B_4139

Structurei

3D structure databases

ProteinModelPortaliP32674
SMRiP32674
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 637PFLPROSITE-ProRule annotationAdd BLAST635
Domaini645 – 765Glycine radicalPROSITE-ProRule annotationAdd BLAST121

Phylogenomic databases

eggNOGiENOG4105C6N Bacteria
COG1882 LUCA
HOGENOMiHOG000274400
InParanoidiP32674
KOiK00656
OMAiMTNRTQR
PhylomeDBiP32674

Family and domain databases

InterProiView protein in InterPro
IPR019777 Form_AcTrfase_GR_CS
IPR001150 Gly_radical
IPR010098 PFL2/GDeHydtase_fam
IPR004184 PFL_dom
PfamiView protein in Pfam
PF01228 Gly_radical, 1 hit
PF02901 PFL-like, 1 hit
TIGRFAMsiTIGR01774 PFL2-3, 1 hit
PROSITEiView protein in PROSITE
PS00850 GLY_RADICAL_1, 1 hit
PS51149 GLY_RADICAL_2, 1 hit
PS51554 PFL, 1 hit

Sequencei

Sequence statusi: Complete.

P32674-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNRISRLKT ALFANTREIS LERALLYTAS HRQTEGEPVI LRRAKATAYI
60 70 80 90 100
LEHVEISIRD EELIAGNRTV KPRAGIMSPE MDPYWLLKEL DQFPTRPQDR
110 120 130 140 150
FAISEEDKRI YREELFPYWE KRSMKDFING QMTDEVKAAT NTQIFSINQT
160 170 180 190 200
DKGQGHIIID YPRLLNHGLG ELVAQMQQHC QQQPENHFYQ AALLLLEASQ
210 220 230 240 250
KHILRYAELA ETMAANCTDA QRREELLTIA EISRHNAQHK PQTFWQACQL
260 270 280 290 300
FWYMNIILQY ESNASSLSLG RFDQYMLPFY QTSLTQGEDA AFLKELLESL
310 320 330 340 350
WVKCNDIVLL RSTSSARYFA GFPTGYTALL GGLTENGRSA VNVLSFLCLD
360 370 380 390 400
AYQSVQLPQP NLGVRTNALI DTPFLMKTAE TIRFGTGIPQ IFNDEVVVPA
410 420 430 440 450
FLNRGVSLED ARDYSVVGCV ELSIPGRTYG LHDIAMFNLL KVMEICLHEN
460 470 480 490 500
EGNAALTYEG LLEQIRAKIS HYITLMVEGS NICDIGHRDW APVPLLSSFI
510 520 530 540 550
SDCLEKGRDI TDGGARYNFS GVQGIGIANL SDSLHALKGM VFEQQRLSFD
560 570 580 590 600
ELLSVLKANF ATPEGEKVRA RLINRFEKYG NDIDEVDNIS AELLRHYCKE
610 620 630 640 650
VEKYQNPRGG YFTPGSYTVS AHVPLGSVVG ATPDGRFAGE QLADGGLSPM
660 670 680 690 700
LGQDAQGPTA VLKSVSKLDN TLLSNGTLLN VKFTPATLEG EAGLRKLADF
710 720 730 740 750
LRAFTQLKLQ HIQFNVVNAD TLREAQQRPQ DYAGLVVRVA GYSAFFVELS
760
KEIQDDIIRR TAHQL
Length:765
Mass (Da):85,960
Last modified:October 1, 1993 - v1
Checksum:i369D6B6E1BBC5BA0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA Translation: AAC43057.1
U00096 Genomic DNA Translation: AAC76933.1
AP009048 Genomic DNA Translation: BAE77360.1
PIRiB65202
RefSeqiNP_418386.1, NC_000913.3
WP_000184811.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76933; AAC76933; b3951
BAE77360; BAE77360; BAE77360
GeneIDi948454
KEGGiecj:JW3923
eco:b3951
PATRICifig|1411691.4.peg.2754

Similar proteinsi

Entry informationi

Entry nameiPFLD_ECOLI
AccessioniPrimary (citable) accession number: P32674
Secondary accession number(s): Q2M8P6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: March 28, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health