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Protein

Formate acetyltransferase 2

Gene

pflD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acetyl-CoA + formate = CoA + pyruvate.

Pathwayi: pyruvate fermentation

This protein is involved in step 1 of the subpathway that synthesizes formate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. Putative formate acetyltransferase 3 (ybiW), Formate acetyltransferase 1 (pflB), Formate acetyltransferase 2 (pflD)
This subpathway is part of the pathway pyruvate fermentation, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes formate from pyruvate, the pathway pyruvate fermentation and in Fermentation.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:EG11910-MONOMER.
ECOL316407:JW3923-MONOMER.
UniPathwayiUPA00920; UER00891.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate acetyltransferase 2 (EC:2.3.1.54)
Alternative name(s):
Pyruvate formate-lyase 2
Gene namesi
Name:pflD
Synonyms:yijL
Ordered Locus Names:b3951, JW3923
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11910. pflD.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001666891 – 765Formate acetyltransferase 2Add BLAST765

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei741Glycine radicalPROSITE-ProRule annotation1

Keywords - PTMi

Organic radical

Proteomic databases

PaxDbiP32674.
PRIDEiP32674.

Interactioni

Protein-protein interaction databases

BioGridi4262065. 2 interactors.
DIPiDIP-10469N.
IntActiP32674. 3 interactors.
STRINGi511145.b3951.

Structurei

3D structure databases

ProteinModelPortaliP32674.
SMRiP32674.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 637PFLPROSITE-ProRule annotationAdd BLAST635
Domaini645 – 765Glycine radicalPROSITE-ProRule annotationAdd BLAST121

Sequence similaritiesi

Contains 1 glycine radical domain.PROSITE-ProRule annotation
Contains 1 PFL domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105C6N. Bacteria.
COG1882. LUCA.
HOGENOMiHOG000274400.
InParanoidiP32674.
KOiK00656.
OMAiEDARDWS.
PhylomeDBiP32674.

Family and domain databases

InterProiIPR019777. Form_AcTrfase_GR_CS.
IPR001150. Gly_radical.
IPR010098. PFL2/GDeHydtase_fam.
IPR004184. PFL_dom.
[Graphical view]
PfamiPF01228. Gly_radical. 1 hit.
PF02901. PFL-like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01774. PFL2-3. 1 hit.
PROSITEiPS00850. GLY_RADICAL_1. 1 hit.
PS51149. GLY_RADICAL_2. 1 hit.
PS51554. PFL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32674-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNRISRLKT ALFANTREIS LERALLYTAS HRQTEGEPVI LRRAKATAYI
60 70 80 90 100
LEHVEISIRD EELIAGNRTV KPRAGIMSPE MDPYWLLKEL DQFPTRPQDR
110 120 130 140 150
FAISEEDKRI YREELFPYWE KRSMKDFING QMTDEVKAAT NTQIFSINQT
160 170 180 190 200
DKGQGHIIID YPRLLNHGLG ELVAQMQQHC QQQPENHFYQ AALLLLEASQ
210 220 230 240 250
KHILRYAELA ETMAANCTDA QRREELLTIA EISRHNAQHK PQTFWQACQL
260 270 280 290 300
FWYMNIILQY ESNASSLSLG RFDQYMLPFY QTSLTQGEDA AFLKELLESL
310 320 330 340 350
WVKCNDIVLL RSTSSARYFA GFPTGYTALL GGLTENGRSA VNVLSFLCLD
360 370 380 390 400
AYQSVQLPQP NLGVRTNALI DTPFLMKTAE TIRFGTGIPQ IFNDEVVVPA
410 420 430 440 450
FLNRGVSLED ARDYSVVGCV ELSIPGRTYG LHDIAMFNLL KVMEICLHEN
460 470 480 490 500
EGNAALTYEG LLEQIRAKIS HYITLMVEGS NICDIGHRDW APVPLLSSFI
510 520 530 540 550
SDCLEKGRDI TDGGARYNFS GVQGIGIANL SDSLHALKGM VFEQQRLSFD
560 570 580 590 600
ELLSVLKANF ATPEGEKVRA RLINRFEKYG NDIDEVDNIS AELLRHYCKE
610 620 630 640 650
VEKYQNPRGG YFTPGSYTVS AHVPLGSVVG ATPDGRFAGE QLADGGLSPM
660 670 680 690 700
LGQDAQGPTA VLKSVSKLDN TLLSNGTLLN VKFTPATLEG EAGLRKLADF
710 720 730 740 750
LRAFTQLKLQ HIQFNVVNAD TLREAQQRPQ DYAGLVVRVA GYSAFFVELS
760
KEIQDDIIRR TAHQL
Length:765
Mass (Da):85,960
Last modified:October 1, 1993 - v1
Checksum:i369D6B6E1BBC5BA0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43057.1.
U00096 Genomic DNA. Translation: AAC76933.1.
AP009048 Genomic DNA. Translation: BAE77360.1.
PIRiB65202.
RefSeqiNP_418386.1. NC_000913.3.
WP_000184811.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76933; AAC76933; b3951.
BAE77360; BAE77360; BAE77360.
GeneIDi948454.
KEGGiecj:JW3923.
eco:b3951.
PATRICi32123423. VBIEscCol129921_4072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43057.1.
U00096 Genomic DNA. Translation: AAC76933.1.
AP009048 Genomic DNA. Translation: BAE77360.1.
PIRiB65202.
RefSeqiNP_418386.1. NC_000913.3.
WP_000184811.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP32674.
SMRiP32674.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262065. 2 interactors.
DIPiDIP-10469N.
IntActiP32674. 3 interactors.
STRINGi511145.b3951.

Proteomic databases

PaxDbiP32674.
PRIDEiP32674.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76933; AAC76933; b3951.
BAE77360; BAE77360; BAE77360.
GeneIDi948454.
KEGGiecj:JW3923.
eco:b3951.
PATRICi32123423. VBIEscCol129921_4072.

Organism-specific databases

EchoBASEiEB1854.
EcoGeneiEG11910. pflD.

Phylogenomic databases

eggNOGiENOG4105C6N. Bacteria.
COG1882. LUCA.
HOGENOMiHOG000274400.
InParanoidiP32674.
KOiK00656.
OMAiEDARDWS.
PhylomeDBiP32674.

Enzyme and pathway databases

UniPathwayiUPA00920; UER00891.
BioCyciEcoCyc:EG11910-MONOMER.
ECOL316407:JW3923-MONOMER.

Miscellaneous databases

PROiP32674.

Family and domain databases

InterProiIPR019777. Form_AcTrfase_GR_CS.
IPR001150. Gly_radical.
IPR010098. PFL2/GDeHydtase_fam.
IPR004184. PFL_dom.
[Graphical view]
PfamiPF01228. Gly_radical. 1 hit.
PF02901. PFL-like. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01774. PFL2-3. 1 hit.
PROSITEiPS00850. GLY_RADICAL_1. 1 hit.
PS51149. GLY_RADICAL_2. 1 hit.
PS51554. PFL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPFLD_ECOLI
AccessioniPrimary (citable) accession number: P32674
Secondary accession number(s): Q2M8P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.