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Protein

Multiphosphoryl transfer protein 2

Gene

ptsA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Multifunctional protein that includes general (non sugar-specific) and sugar-specific components of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II FrwABC PTS system is involved in fructose transport.1 Publication

Catalytic activityi

Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine.By similarity
[Protein]-N(pi)-phospho-L-histidine + D-fructose(Side 1) = [protein]-L-histidine + D-fructose 1-phosphate(Side 2).By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei16Tele-phosphohistidine intermediate; for HPr activityPROSITE-ProRule annotation1
Active sitei301Tele-phosphohistidine intermediate; for PTS EI activityPROSITE-ProRule annotationBy similarity1
Binding sitei408PEPBy similarity1
Binding sitei444PEPBy similarity1
Metal bindingi543MagnesiumBy similarity1
Metal bindingi567MagnesiumBy similarity1
Binding sitei577PEPBy similarity1
Active sitei614Proton donor; for EI activityBy similarity1
Active sitei750Tele-phosphohistidine intermediate; for PTS EIIA activityPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11906-MONOMER.
ECOL316407:JW5555-MONOMER.

Protein family/group databases

TCDBi4.A.2.1.10. the pts fructose-mannitol (fru) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Multiphosphoryl transfer protein 21 Publication
Short name:
MTP 21 Publication
Alternative name(s):
Triphosphoryl transfer protein 2By similarity
Short name:
TTP 2By similarity
Including the following 3 domains:
Phosphoenolpyruvate-protein phosphotransferase1 Publication (EC:2.7.3.9By similarity)
Alternative name(s):
Enzyme I-Ani1 Publication
Phosphotransferase system enzyme I1 Publication
Phosphocarrier protein HPrBy similarity
Short name:
Protein HBy similarity
PTS system fructose-like EIIA component1 Publication (EC:2.7.1.202By similarity)
Alternative name(s):
Fructose-like phosphotransferase enzyme IIA component1 Publication
Gene namesi
Name:ptsA1 Publication
Synonyms:frwA, yijH
Ordered Locus Names:b3947, JW5555
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11906. ptsA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001470941 – 833Multiphosphoryl transfer protein 2Add BLAST833

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16Phosphohistidine; by EICurated1
Modified residuei301Phosphohistidine; by autocatalysisCurated1
Modified residuei750Phosphohistidine; by HPrCurated1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP32670.
PRIDEiP32670.

Interactioni

Protein-protein interaction databases

BioGridi4262062. 11 interactors.
DIPiDIP-10601N.
STRINGi511145.b3947.

Structurei

3D structure databases

ProteinModelPortaliP32670.
SMRiP32670.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 91HPrPROSITE-ProRule annotationAdd BLAST90
Domaini688 – 830PTS EIIA type-2PROSITE-ProRule annotationAdd BLAST143

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni143 – 653PTS EI1 PublicationAdd BLAST511
Regioni566 – 567PEP bindingBy similarity2

Domaini

The PTS EIIA type-2 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-2 domain.PROSITE-ProRule annotation
In contrast to classical PTS systems, the fructose-like PTS has no requirement for HPr and Enzyme I; PtsA combines a IIA domain with an Enzyme I and a HPr domains.Curated

Sequence similaritiesi

Belongs to the PEP-utilizing enzyme family.Curated
Contains 1 HPr domain.PROSITE-ProRule annotation
Contains 1 PTS EIIA type-2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105BZ3. Bacteria.
COG1080. LUCA.
COG1762. LUCA.
COG1925. LUCA.
HOGENOMiHOG000122933.
InParanoidiP32670.
KOiK08483.
K11189.
K11201.
OMAiHIYGEQR.
PhylomeDBiP32670.

Family and domain databases

CDDicd00367. PTS-HPr_like. 1 hit.
Gene3Di1.10.274.10. 1 hit.
3.20.20.60. 1 hit.
3.30.1340.10. 1 hit.
3.40.930.10. 1 hit.
3.50.30.10. 1 hit.
InterProiIPR000032. HPr-like.
IPR008279. PEP-util_enz_mobile_dom.
IPR018274. PEP_util_AS.
IPR000121. PEP_util_C.
IPR023151. PEP_util_CS.
IPR016152. PTrfase/Anion_transptr.
IPR006318. PTS_EI-like.
IPR002178. PTS_EIIA_type-2_dom.
IPR008731. PTS_EIN.
IPR001020. PTS_HPr_His_P_site.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF05524. PEP-utilisers_N. 1 hit.
PF00391. PEP-utilizers. 1 hit.
PF02896. PEP-utilizers_C. 1 hit.
PF00381. PTS-HPr. 1 hit.
PF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
SUPFAMiSSF47831. SSF47831. 1 hit.
SSF51621. SSF51621. 1 hit.
SSF52009. SSF52009. 1 hit.
SSF55594. SSF55594. 1 hit.
SSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR01417. PTS_I_fam. 1 hit.
PROSITEiPS00742. PEP_ENZYMES_2. 1 hit.
PS00370. PEP_ENZYMES_PHOS_SITE. 1 hit.
PS51094. PTS_EIIA_TYPE_2. 1 hit.
PS51350. PTS_HPR_DOM. 1 hit.
PS00369. PTS_HPR_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32670-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALIVEFICE LPNGVHARPA SHVETLCNTF SSQIEWHNLR TDRKGNAKSA
60 70 80 90 100
LALIGTDTLA GDNCQLLISG ADEQEAHQRL SQWLRDEFPH CDAPLAEVKS
110 120 130 140 150
DELEPLPVSL TNLNPQIIRA RTVCSGSAGG ILTPISSLDL NALGNLPAAK
160 170 180 190 200
GVDAEQSALE NGLTLVLKNI EFRLLDSDGA TSAILEAHRS LAGDTSLREH
210 220 230 240 250
LLAGVSAGLS CAEAIVASAN HFCEEFSRSS SSYLQERALD VRDVCFQLLQ
260 270 280 290 300
QIYGEQRFPA PGKLTQPAIC MADELTPSQF LELDKNHLKG LLLKSGGTTS
310 320 330 340 350
HTVILARSFN IPTLVGVDID ALTPWQQQTI YIDGNAGAIV VEPGEAVARY
360 370 380 390 400
YQQEARVQDA LREQQRVWLT QQARTADGIR IEIAANIAHS VEAQAAFGNG
410 420 430 440 450
AEGVGLFRTE MLYMDRTSAP GESELYNIFC QALESANGRS IIVRTMDIGG
460 470 480 490 500
DKPVDYLNIP AEANPFLGYR AVRIYEEYAS LFTTQLRSIL RASAHGSLKI
510 520 530 540 550
MIPMISSMEE ILWVKEKLAE AKQQLRNEHI PFDEKIQLGI MLEVPSVMFI
560 570 580 590 600
IDQCCEEIDF FSIGSNDLTQ YLLAVDRDNA KVTRHYNSLN PAFLRALDYA
610 620 630 640 650
VQAVHRQGKW IGLCGELGAK GSVLPLLVGL GLDELSMSAP SIPAAKARMA
660 670 680 690 700
QLDSRECRKL LNQAMACRTS LEVEHLLAQF RMTQQDAPLV TAECITLESD
710 720 730 740 750
WRSKEEVLKG MTDNLLLAGR CRYPRKLEAD LWAREAVFST GLGFSFAIPH
760 770 780 790 800
SKSEHIEQST ISVARLQAPV RWGDDEAQFI IMLTLNKHAA GDQHMRIFSR
810 820 830
LARRIMHEEF RNALVNAASA DAIASLLQHE LEL
Length:833
Mass (Da):91,774
Last modified:April 27, 2001 - v2
Checksum:i8E667F44D29CC732
GO

Sequence cautioni

The sequence AAC43053 differs from that shown. Reason: Frameshift at position 45.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43053.1. Frameshift.
U00096 Genomic DNA. Translation: AAT48236.1.
AP009048 Genomic DNA. Translation: BAE77363.1.
RefSeqiWP_001174077.1. NZ_LN832404.1.
YP_026278.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48236; AAT48236; b3947.
BAE77363; BAE77363; BAE77363.
GeneIDi948437.
KEGGiecj:JW5555.
eco:b3947.
PATRICi32123417. VBIEscCol129921_4069.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43053.1. Frameshift.
U00096 Genomic DNA. Translation: AAT48236.1.
AP009048 Genomic DNA. Translation: BAE77363.1.
RefSeqiWP_001174077.1. NZ_LN832404.1.
YP_026278.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP32670.
SMRiP32670.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262062. 11 interactors.
DIPiDIP-10601N.
STRINGi511145.b3947.

Protein family/group databases

TCDBi4.A.2.1.10. the pts fructose-mannitol (fru) family.

Proteomic databases

PaxDbiP32670.
PRIDEiP32670.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48236; AAT48236; b3947.
BAE77363; BAE77363; BAE77363.
GeneIDi948437.
KEGGiecj:JW5555.
eco:b3947.
PATRICi32123417. VBIEscCol129921_4069.

Organism-specific databases

EchoBASEiEB1851.
EcoGeneiEG11906. ptsA.

Phylogenomic databases

eggNOGiENOG4105BZ3. Bacteria.
COG1080. LUCA.
COG1762. LUCA.
COG1925. LUCA.
HOGENOMiHOG000122933.
InParanoidiP32670.
KOiK08483.
K11189.
K11201.
OMAiHIYGEQR.
PhylomeDBiP32670.

Enzyme and pathway databases

BioCyciEcoCyc:EG11906-MONOMER.
ECOL316407:JW5555-MONOMER.

Miscellaneous databases

PROiP32670.

Family and domain databases

CDDicd00367. PTS-HPr_like. 1 hit.
Gene3Di1.10.274.10. 1 hit.
3.20.20.60. 1 hit.
3.30.1340.10. 1 hit.
3.40.930.10. 1 hit.
3.50.30.10. 1 hit.
InterProiIPR000032. HPr-like.
IPR008279. PEP-util_enz_mobile_dom.
IPR018274. PEP_util_AS.
IPR000121. PEP_util_C.
IPR023151. PEP_util_CS.
IPR016152. PTrfase/Anion_transptr.
IPR006318. PTS_EI-like.
IPR002178. PTS_EIIA_type-2_dom.
IPR008731. PTS_EIN.
IPR001020. PTS_HPr_His_P_site.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF05524. PEP-utilisers_N. 1 hit.
PF00391. PEP-utilizers. 1 hit.
PF02896. PEP-utilizers_C. 1 hit.
PF00381. PTS-HPr. 1 hit.
PF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
SUPFAMiSSF47831. SSF47831. 1 hit.
SSF51621. SSF51621. 1 hit.
SSF52009. SSF52009. 1 hit.
SSF55594. SSF55594. 1 hit.
SSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR01417. PTS_I_fam. 1 hit.
PROSITEiPS00742. PEP_ENZYMES_2. 1 hit.
PS00370. PEP_ENZYMES_PHOS_SITE. 1 hit.
PS51094. PTS_EIIA_TYPE_2. 1 hit.
PS51350. PTS_HPR_DOM. 1 hit.
PS00369. PTS_HPR_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTFX2_ECOLI
AccessioniPrimary (citable) accession number: P32670
Secondary accession number(s): Q2M8P3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 27, 2001
Last modified: November 2, 2016
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.