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Protein

Phospholipid-transporting ATPase DNF1

Gene

DNF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids.Curated

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei6674-aspartylphosphate intermediateBy similarity1

GO - Molecular functioni

  • ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Source: SGD
  • ATP binding Source: UniProtKB-KW
  • magnesium ion binding Source: InterPro
  • phospholipid-translocating ATPase activity Source: SGD

GO - Biological processi

  • endocytosis Source: SGD
  • establishment or maintenance of cell polarity Source: SGD
  • intracellular protein transport Source: SGD
  • phospholipid translocation Source: SGD
  • response to pheromone involved in conjugation with cellular fusion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30327-MONOMER.
BRENDAi3.6.3.1. 984.
ReactomeiR-SCE-6798695. Neutrophil degranulation.
R-SCE-936837. Ion transport by P-type ATPases.

Protein family/group databases

TCDBi3.A.3.8.4. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid-transporting ATPase DNF1 (EC:3.6.3.1)
Alternative name(s):
Flippase DNF1
Gene namesi
Name:DNF1
Ordered Locus Names:YER166W
ORF Names:SYGP-ORF7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER166W.
SGDiS000000968. DNF1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 214CytoplasmicSequence analysisAdd BLAST214
Transmembranei215 – 235HelicalSequence analysisAdd BLAST21
Topological domaini236 – 239ExtracellularSequence analysis4
Transmembranei240 – 260HelicalSequence analysisAdd BLAST21
Topological domaini261 – 553CytoplasmicSequence analysisAdd BLAST293
Transmembranei554 – 574HelicalSequence analysisAdd BLAST21
Topological domaini575 – 594ExtracellularSequence analysisAdd BLAST20
Transmembranei595 – 615HelicalSequence analysisAdd BLAST21
Topological domaini616 – 1188CytoplasmicSequence analysisAdd BLAST573
Transmembranei1189 – 1209HelicalSequence analysisAdd BLAST21
Topological domaini1210 – 1219ExtracellularSequence analysis10
Transmembranei1220 – 1240HelicalSequence analysisAdd BLAST21
Topological domaini1241 – 1270CytoplasmicSequence analysisAdd BLAST30
Transmembranei1271 – 1291HelicalSequence analysisAdd BLAST21
Topological domaini1292 – 1307ExtracellularSequence analysisAdd BLAST16
Transmembranei1308 – 1328HelicalSequence analysisAdd BLAST21
Topological domaini1329 – 1334CytoplasmicSequence analysis6
Transmembranei1335 – 1355HelicalSequence analysisAdd BLAST21
Topological domaini1356 – 1375ExtracellularSequence analysisAdd BLAST20
Transmembranei1376 – 1396HelicalSequence analysisAdd BLAST21
Topological domaini1397 – 1571CytoplasmicSequence analysisAdd BLAST175

GO - Cellular componenti

  • endosome membrane Source: UniProtKB-SubCell
  • Golgi apparatus Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • Lem3p-Dnf1p complex Source: SGD
  • mating projection tip membrane Source: SGD
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462351 – 1571Phospholipid-transporting ATPase DNF1Add BLAST1571

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53PhosphoserineCombined sources1
Modified residuei70PhosphothreonineCombined sources1
Modified residuei81PhosphoserineCombined sources1
Modified residuei85PhosphothreonineCombined sources1
Modified residuei92PhosphoserineCombined sources1
Modified residuei94PhosphothreonineCombined sources1
Modified residuei104PhosphoserineCombined sources1
Modified residuei109PhosphothreonineCombined sources1
Modified residuei351PhosphoserineCombined sources1
Modified residuei354PhosphoserineBy similarity1
Modified residuei358PhosphoserineBy similarity1
Modified residuei365PhosphoserineCombined sources1
Modified residuei368PhosphotyrosineBy similarity1
Cross-linki895Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei1506PhosphoserineCombined sources1
Modified residuei1551PhosphothreonineCombined sources1
Modified residuei1552PhosphoserineCombined sources1
Modified residuei1563PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by FPK1 and KIN82.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP32660.
PRIDEiP32660.

PTM databases

iPTMnetiP32660.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
LEM3P428384EBI-3121,EBI-28396

Protein-protein interaction databases

BioGridi36919. 46 interactors.
DIPiDIP-7949N.
IntActiP32660. 16 interactors.
MINTiMINT-1364907.

Structurei

3D structure databases

ProteinModelPortaliP32660.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00860000133775.
HOGENOMiHOG000202528.
InParanoidiP32660.
KOiK01530.
OMAiFWAVFFV.
OrthoDBiEOG092C0BQ4.

Family and domain databases

Gene3Di2.70.150.10. 3 hits.
3.40.1110.10. 2 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
IPR026871. PLip_transp_ATPase.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 2 hits.
PTHR24092:SF87. PTHR24092:SF87. 2 hits.
PfamiPF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 2 hits.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32660-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGTFHGDGH APMSPFEDTF QFEDNSSNED THIAPTHFDD GATSNKYSRP
60 70 80 90 100
QVSFNDETPK NKREDAEEFT FNDDTEYDNH SFQPTPKLNN GSGTFDDVEL
110 120 130 140 150
DNDSGEPHTN YDGMKRFRMG TKRNKKGNPI MGRSKTLKWA RKNIPNPFED
160 170 180 190 200
FTKDDIDPGA INRAQELRTV YYNMPLPKDM IDEEGNPIMQ YPRNKIRTTK
210 220 230 240 250
YTPLTFLPKN ILFQFHNFAN VYFLVLIILG AFQIFGVTNP GLSAVPLVVI
260 270 280 290 300
VIITAIKDAI EDSRRTVLDL EVNNTKTHIL EGVENENVST DNISLWRRFK
310 320 330 340 350
KANSRLLFKF IQYCKEHLTE EGKKKRMQRK RHELRVQKTV GTSGPRSSLD
360 370 380 390 400
SIDSYRVSAD YGRPSLDYDN LEQGAGEANI VDRSLPPRTD CKFAKNYWKG
410 420 430 440 450
VKVGDIVRIH NNDEIPADII LLSTSDTDGA CYVETKNLDG ETNLKVRQSL
460 470 480 490 500
KCTNTIRTSK DIARTKFWIE SEGPHSNLYT YQGNMKWRNL ADGEIRNEPI
510 520 530 540 550
TINNVLLRGC TLRNTKWAMG VVMFTGGDTK IMLNSGITPT KKSRISRELN
560 570 580 590 600
FSVVINFVLL FILCFVSGIA NGVYYDKKGR SRFSYEFGTI AGSAATNGFV
610 620 630 640 650
SFWVAVILYQ SLVPISLYIS VEIIKTAQAA FIYGDVLLYN AKLDYPCTPK
660 670 680 690 700
SWNISDDLGQ VEYIFSDKTG TLTQNVMEFK KCTINGVSYG RAYTEALAGL
710 720 730 740 750
RKRQGIDVET EGRREKAEIA KDRDTMIDEL RALSGNSQFY PEEVTFVSKE
760 770 780 790 800
FVRDLKGASG EVQQRCCEHF MLALALCHSV LVEANPDNPK KLDLKAQSPD
810 820 830 840 850
EAALVATARD VGFSFVGKTK KGLIIEMQGI QKEFEILNIL EFNSSRKRMS
860 870 880 890 900
CIVKIPGLNP GDEPRALLIC KGADSIIYSR LSRQSGSNSE AILEKTALHL
910 920 930 940 950
EQYATEGLRT LCIAQRELSW SEYEKWNEKY DIAAASLANR EDELEVVADS
960 970 980 990 1000
IERELILLGG TAIEDRLQDG VPDCIELLAE AGIKLWVLTG DKVETAINIG
1010 1020 1030 1040 1050
FSCNLLNNEM ELLVIKTTGD DVKEFGSEPS EIVDALLSKY LKEYFNLTGS
1060 1070 1080 1090 1100
EEEIFEAKKD HEFPKGNYAI VIDGDALKLA LYGEDIRRKF LLLCKNCRAV
1110 1120 1130 1140 1150
LCCRVSPSQK AAVVKLVKDS LDVMTLAIGD GSNDVAMIQS ADVGIGIAGE
1160 1170 1180 1190 1200
EGRQAVMCSD YAIGQFRYLA RLVLVHGRWS YKRLAEMIPE FFYKNMIFAL
1210 1220 1230 1240 1250
ALFWYGIYND FDGSYLYEYT YMMFYNLAFT SLPVIFLGIL DQDVNDTISL
1260 1270 1280 1290 1300
VVPQLYRVGI LRKEWNQRKF LWYMLDGLYQ SIICFFFPYL VYHKNMIVTS
1310 1320 1330 1340 1350
NGLGLDHRYF VGVYVTTIAV ISCNTYVLLH QYRWDWFSGL FIALSCLVVF
1360 1370 1380 1390 1400
AWTGIWSSAI ASREFFKAAA RIYGAPSFWA VFFVAVLFCL LPRFTYDSFQ
1410 1420 1430 1440 1450
KFFYPTDVEI VREMWQHGHF DHYPPGYDPT DPNRPKVTKA GQHGEKIIEG
1460 1470 1480 1490 1500
IALSDNLGGS NYSRDSVVTE EIPMTFMHGE DGSPSGYQKQ ETWMTSPKET
1510 1520 1530 1540 1550
QDLLQSPQFQ QAQTFGRGPS TNVRSSLDRT REQMIATNQL DNRYSVERAR
1560 1570
TSLDLPGVTN AASLIGTQQN N
Length:1,571
Mass (Da):177,798
Last modified:February 1, 1995 - v2
Checksum:i3CC3FBA8ADDE8960
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18922 Genomic DNA. Translation: AAB64693.1.
BK006939 Genomic DNA. Translation: DAA07828.1.
PIRiS50669.
RefSeqiNP_011093.3. NM_001179056.3.

Genome annotation databases

EnsemblFungiiYER166W; YER166W; YER166W.
GeneIDi856913.
KEGGisce:YER166W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18922 Genomic DNA. Translation: AAB64693.1.
BK006939 Genomic DNA. Translation: DAA07828.1.
PIRiS50669.
RefSeqiNP_011093.3. NM_001179056.3.

3D structure databases

ProteinModelPortaliP32660.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36919. 46 interactors.
DIPiDIP-7949N.
IntActiP32660. 16 interactors.
MINTiMINT-1364907.

Protein family/group databases

TCDBi3.A.3.8.4. the p-type atpase (p-atpase) superfamily.

PTM databases

iPTMnetiP32660.

Proteomic databases

MaxQBiP32660.
PRIDEiP32660.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER166W; YER166W; YER166W.
GeneIDi856913.
KEGGisce:YER166W.

Organism-specific databases

EuPathDBiFungiDB:YER166W.
SGDiS000000968. DNF1.

Phylogenomic databases

GeneTreeiENSGT00860000133775.
HOGENOMiHOG000202528.
InParanoidiP32660.
KOiK01530.
OMAiFWAVFFV.
OrthoDBiEOG092C0BQ4.

Enzyme and pathway databases

BioCyciYEAST:G3O-30327-MONOMER.
BRENDAi3.6.3.1. 984.
ReactomeiR-SCE-6798695. Neutrophil degranulation.
R-SCE-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiP32660.

Family and domain databases

Gene3Di2.70.150.10. 3 hits.
3.40.1110.10. 2 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
IPR026871. PLip_transp_ATPase.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 2 hits.
PTHR24092:SF87. PTHR24092:SF87. 2 hits.
PfamiPF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 2 hits.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATC5_YEAST
AccessioniPrimary (citable) accession number: P32660
Secondary accession number(s): D3DM74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 166 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.