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P32660

- ATC5_YEAST

UniProt

P32660 - ATC5_YEAST

Protein

Phospholipid-transporting ATPase DNF1

Gene

DNF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids.Curated

    Catalytic activityi

    ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei667 – 66714-aspartylphosphate intermediateBy similarity

    GO - Molecular functioni

    1. ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Source: SGD
    2. ATP binding Source: UniProtKB-KW
    3. cation-transporting ATPase activity Source: InterPro
    4. magnesium ion binding Source: InterPro
    5. phospholipid-translocating ATPase activity Source: SGD
    6. protein binding Source: IntAct

    GO - Biological processi

    1. endocytosis Source: SGD
    2. establishment or maintenance of cell polarity Source: SGD
    3. intracellular protein transport Source: SGD
    4. ion transmembrane transport Source: GOC
    5. phospholipid translocation Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30327-MONOMER.
    ReactomeiREACT_96459. Ion transport by P-type ATPases.

    Protein family/group databases

    TCDBi3.A.3.8.4. the p-type atpase (p-atpase) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phospholipid-transporting ATPase DNF1 (EC:3.6.3.1)
    Alternative name(s):
    Flippase DNF1
    Gene namesi
    Name:DNF1
    Ordered Locus Names:YER166W
    ORF Names:SYGP-ORF7
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER166w.
    SGDiS000000968. DNF1.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Endosome membrane 1 Publication; Multi-pass membrane protein 1 Publication. Golgi apparatustrans-Golgi network membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. endosome membrane Source: UniProtKB-SubCell
    2. Golgi apparatus Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Endosome, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15711571Phospholipid-transporting ATPase DNF1PRO_0000046235Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei53 – 531Phosphoserine4 Publications
    Modified residuei70 – 701Phosphothreonine2 Publications
    Modified residuei81 – 811Phosphoserine3 Publications
    Modified residuei85 – 851Phosphothreonine3 Publications
    Modified residuei92 – 921Phosphoserine2 Publications
    Modified residuei94 – 941Phosphothreonine2 Publications
    Modified residuei104 – 1041Phosphoserine2 Publications
    Modified residuei109 – 1091Phosphothreonine2 Publications
    Modified residuei351 – 3511Phosphoserine2 Publications
    Modified residuei365 – 3651Phosphoserine2 Publications
    Modified residuei1506 – 15061Phosphoserine4 Publications
    Modified residuei1551 – 15511Phosphothreonine2 Publications
    Modified residuei1552 – 15521Phosphoserine2 Publications
    Modified residuei1563 – 15631Phosphoserine2 Publications

    Post-translational modificationi

    Phosphorylated by FPK1 and KIN82.5 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP32660.
    PaxDbiP32660.
    PeptideAtlasiP32660.

    Expressioni

    Gene expression databases

    GenevestigatoriP32660.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LEM3P428384EBI-3121,EBI-28396

    Protein-protein interaction databases

    BioGridi36919. 36 interactions.
    DIPiDIP-7949N.
    IntActiP32660. 16 interactions.
    MINTiMINT-1364907.
    STRINGi4932.YER166W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32660.
    SMRiP32660. Positions 1107-1174.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 214214CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini236 – 2394ExtracellularSequence Analysis
    Topological domaini261 – 553293CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini575 – 59420ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini616 – 1188573CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1210 – 121910ExtracellularSequence Analysis
    Topological domaini1241 – 127030CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1292 – 130716ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1329 – 13346CytoplasmicSequence Analysis
    Topological domaini1356 – 137520ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1397 – 1571175CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei215 – 23521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei240 – 26021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei554 – 57421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei595 – 61521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1189 – 120921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1220 – 124021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1271 – 129121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1308 – 132821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1335 – 135521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1376 – 139621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    GeneTreeiENSGT00740000115303.
    HOGENOMiHOG000202528.
    KOiK01530.
    OMAiTHILEGV.
    OrthoDBiEOG7FR7QR.

    Family and domain databases

    Gene3Di2.70.150.10. 3 hits.
    3.40.1110.10. 2 hits.
    3.40.50.1000. 2 hits.
    InterProiIPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR006539. ATPase_P-typ_Plipid-transp.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    IPR026871. PLip_transp_ATPase.
    [Graphical view]
    PANTHERiPTHR24092. PTHR24092. 1 hit.
    PTHR24092:SF7. PTHR24092:SF7. 1 hit.
    PfamiPF00122. E1-E2_ATPase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SUPFAMiSSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 2 hits.
    TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32660-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGTFHGDGH APMSPFEDTF QFEDNSSNED THIAPTHFDD GATSNKYSRP     50
    QVSFNDETPK NKREDAEEFT FNDDTEYDNH SFQPTPKLNN GSGTFDDVEL 100
    DNDSGEPHTN YDGMKRFRMG TKRNKKGNPI MGRSKTLKWA RKNIPNPFED 150
    FTKDDIDPGA INRAQELRTV YYNMPLPKDM IDEEGNPIMQ YPRNKIRTTK 200
    YTPLTFLPKN ILFQFHNFAN VYFLVLIILG AFQIFGVTNP GLSAVPLVVI 250
    VIITAIKDAI EDSRRTVLDL EVNNTKTHIL EGVENENVST DNISLWRRFK 300
    KANSRLLFKF IQYCKEHLTE EGKKKRMQRK RHELRVQKTV GTSGPRSSLD 350
    SIDSYRVSAD YGRPSLDYDN LEQGAGEANI VDRSLPPRTD CKFAKNYWKG 400
    VKVGDIVRIH NNDEIPADII LLSTSDTDGA CYVETKNLDG ETNLKVRQSL 450
    KCTNTIRTSK DIARTKFWIE SEGPHSNLYT YQGNMKWRNL ADGEIRNEPI 500
    TINNVLLRGC TLRNTKWAMG VVMFTGGDTK IMLNSGITPT KKSRISRELN 550
    FSVVINFVLL FILCFVSGIA NGVYYDKKGR SRFSYEFGTI AGSAATNGFV 600
    SFWVAVILYQ SLVPISLYIS VEIIKTAQAA FIYGDVLLYN AKLDYPCTPK 650
    SWNISDDLGQ VEYIFSDKTG TLTQNVMEFK KCTINGVSYG RAYTEALAGL 700
    RKRQGIDVET EGRREKAEIA KDRDTMIDEL RALSGNSQFY PEEVTFVSKE 750
    FVRDLKGASG EVQQRCCEHF MLALALCHSV LVEANPDNPK KLDLKAQSPD 800
    EAALVATARD VGFSFVGKTK KGLIIEMQGI QKEFEILNIL EFNSSRKRMS 850
    CIVKIPGLNP GDEPRALLIC KGADSIIYSR LSRQSGSNSE AILEKTALHL 900
    EQYATEGLRT LCIAQRELSW SEYEKWNEKY DIAAASLANR EDELEVVADS 950
    IERELILLGG TAIEDRLQDG VPDCIELLAE AGIKLWVLTG DKVETAINIG 1000
    FSCNLLNNEM ELLVIKTTGD DVKEFGSEPS EIVDALLSKY LKEYFNLTGS 1050
    EEEIFEAKKD HEFPKGNYAI VIDGDALKLA LYGEDIRRKF LLLCKNCRAV 1100
    LCCRVSPSQK AAVVKLVKDS LDVMTLAIGD GSNDVAMIQS ADVGIGIAGE 1150
    EGRQAVMCSD YAIGQFRYLA RLVLVHGRWS YKRLAEMIPE FFYKNMIFAL 1200
    ALFWYGIYND FDGSYLYEYT YMMFYNLAFT SLPVIFLGIL DQDVNDTISL 1250
    VVPQLYRVGI LRKEWNQRKF LWYMLDGLYQ SIICFFFPYL VYHKNMIVTS 1300
    NGLGLDHRYF VGVYVTTIAV ISCNTYVLLH QYRWDWFSGL FIALSCLVVF 1350
    AWTGIWSSAI ASREFFKAAA RIYGAPSFWA VFFVAVLFCL LPRFTYDSFQ 1400
    KFFYPTDVEI VREMWQHGHF DHYPPGYDPT DPNRPKVTKA GQHGEKIIEG 1450
    IALSDNLGGS NYSRDSVVTE EIPMTFMHGE DGSPSGYQKQ ETWMTSPKET 1500
    QDLLQSPQFQ QAQTFGRGPS TNVRSSLDRT REQMIATNQL DNRYSVERAR 1550
    TSLDLPGVTN AASLIGTQQN N 1571
    Length:1,571
    Mass (Da):177,798
    Last modified:February 1, 1995 - v2
    Checksum:i3CC3FBA8ADDE8960
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18922 Genomic DNA. Translation: AAB64693.1.
    BK006939 Genomic DNA. Translation: DAA07828.1.
    PIRiS50669.
    RefSeqiNP_011093.3. NM_001179056.3.

    Genome annotation databases

    EnsemblFungiiYER166W; YER166W; YER166W.
    GeneIDi856913.
    KEGGisce:YER166W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18922 Genomic DNA. Translation: AAB64693.1 .
    BK006939 Genomic DNA. Translation: DAA07828.1 .
    PIRi S50669.
    RefSeqi NP_011093.3. NM_001179056.3.

    3D structure databases

    ProteinModelPortali P32660.
    SMRi P32660. Positions 1107-1174.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36919. 36 interactions.
    DIPi DIP-7949N.
    IntActi P32660. 16 interactions.
    MINTi MINT-1364907.
    STRINGi 4932.YER166W.

    Protein family/group databases

    TCDBi 3.A.3.8.4. the p-type atpase (p-atpase) superfamily.

    Proteomic databases

    MaxQBi P32660.
    PaxDbi P32660.
    PeptideAtlasi P32660.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER166W ; YER166W ; YER166W .
    GeneIDi 856913.
    KEGGi sce:YER166W.

    Organism-specific databases

    CYGDi YER166w.
    SGDi S000000968. DNF1.

    Phylogenomic databases

    eggNOGi COG0474.
    GeneTreei ENSGT00740000115303.
    HOGENOMi HOG000202528.
    KOi K01530.
    OMAi THILEGV.
    OrthoDBi EOG7FR7QR.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30327-MONOMER.
    Reactomei REACT_96459. Ion transport by P-type ATPases.

    Miscellaneous databases

    NextBioi 983359.

    Gene expression databases

    Genevestigatori P32660.

    Family and domain databases

    Gene3Di 2.70.150.10. 3 hits.
    3.40.1110.10. 2 hits.
    3.40.50.1000. 2 hits.
    InterProi IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR006539. ATPase_P-typ_Plipid-transp.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    IPR026871. PLip_transp_ATPase.
    [Graphical view ]
    PANTHERi PTHR24092. PTHR24092. 1 hit.
    PTHR24092:SF7. PTHR24092:SF7. 1 hit.
    Pfami PF00122. E1-E2_ATPase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SUPFAMi SSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 2 hits.
    TIGRFAMsi TIGR01652. ATPase-Plipid. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    4. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81; THR-85 AND SER-1506, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    5. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-53, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Protein kinases Fpk1p and Fpk2p are novel regulators of phospholipid asymmetry."
      Nakano K., Yamamoto T., Kishimoto T., Noji T., Tanaka K.
      Mol. Biol. Cell 19:1783-1797(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION BY FPK1 AND KIN82.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-53; SER-351 AND SER-1506, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-53; THR-70; SER-81; THR-85; SER-92; THR-94; SER-104; THR-109; SER-365; SER-1506; THR-1551; SER-1552 AND SER-1563, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiATC5_YEAST
    AccessioniPrimary (citable) accession number: P32660
    Secondary accession number(s): D3DM74
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 142 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3