Reviewed,
UniProtKB/Swiss-Prot P32660 (ATC5_YEAST)
Last modified
February 9, 2010.
Version 97.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Probable phospholipid-transporting ATPase DNF1 EC=3.6.3.1 | ||||||
| Gene names |
| ||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 1571 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids Potential. |
| Catalytic activity | ATP + H2O + phospholipid(In) = ADP + phosphate + phospholipid(Out). |
| Subcellular location | |
| Sequence similarities | Belongs to the cation transport ATPase (P-type) family. Type IV subfamily. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| itself | 1 | EBI-3121,EBI-3121 | ||
| CHS3 | P29465 | 1 | EBI-3121,EBI-4632 | |
| CHS5 | Q12114 | 1 | EBI-3121,EBI-4640 | |
| DNF2 | Q12675 | 1 | EBI-3121,EBI-3114 | |
| DRS2 | P39524 | 1 | EBI-3121,EBI-3106 | |
| FTH1 | P38310 | 1 | EBI-3121,EBI-20959 | |
| KEX1 | P09620 | 1 | EBI-3121,EBI-9653 | |
| KEX2 | P13134 | 1 | EBI-3121,EBI-9658 | |
| LEM3 | P42838 | 2 | EBI-3121,EBI-28396 | |
| LSB3 | P43603 | 1 | EBI-3121,EBI-22980 | |
| PIN2 | Q12057 | 1 | EBI-3121,EBI-29838 | |
| SEC6 | P32844 | 1 | EBI-3121,EBI-16874 | |
| SEC7 | P11075 | 1 | EBI-3121,EBI-16882 | |
| SMI1 | P32566 | 1 | EBI-3121,EBI-17452 | |
| SYP1 | P25623 | 1 | EBI-3121,EBI-21900 | |
| YAP1802 | P53309 | 1 | EBI-3121,EBI-23557 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1571 | 1571 | Probable phospholipid-transporting ATPase DNF1 | PRO_0000046235 | |||||
Regions | |||||||||
| Topological domain | 1 – 214 | 214 | Cytoplasmic Potential | ||||||
| Transmembrane | 215 – 235 | 21 | Potential | ||||||
| Topological domain | 236 – 239 | 4 | Extracellular Potential | ||||||
| Transmembrane | 240 – 260 | 21 | Potential | ||||||
| Topological domain | 261 – 553 | 293 | Cytoplasmic Potential | ||||||
| Transmembrane | 554 – 574 | 21 | Potential | ||||||
| Topological domain | 575 – 594 | 20 | Extracellular Potential | ||||||
| Transmembrane | 595 – 615 | 21 | Potential | ||||||
| Topological domain | 616 – 1188 | 573 | Cytoplasmic Potential | ||||||
| Transmembrane | 1189 – 1209 | 21 | Potential | ||||||
| Topological domain | 1210 – 1219 | 10 | Extracellular Potential | ||||||
| Transmembrane | 1220 – 1240 | 21 | Potential | ||||||
| Topological domain | 1241 – 1270 | 30 | Cytoplasmic Potential | ||||||
| Transmembrane | 1271 – 1291 | 21 | Potential | ||||||
| Topological domain | 1292 – 1307 | 16 | Extracellular Potential | ||||||
| Transmembrane | 1308 – 1328 | 21 | Potential | ||||||
| Topological domain | 1329 – 1334 | 6 | Cytoplasmic Potential | ||||||
| Transmembrane | 1335 – 1355 | 21 | Potential | ||||||
| Topological domain | 1356 – 1375 | 20 | Extracellular Potential | ||||||
| Transmembrane | 1376 – 1396 | 21 | Potential | ||||||
| Topological domain | 1397 – 1571 | 175 | Cytoplasmic Potential | ||||||
Sites | |||||||||
| Active site | 667 | 1 | 4-aspartylphosphate intermediate By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 53 | 1 | Phosphoserine Ref.4 Ref.5 Ref.6 | ||||||
| Modified residue | 58 | 1 | Phosphothreonine Ref.6 | ||||||
| Modified residue | 77 | 1 | Phosphotyrosine Ref.6 | ||||||
| Modified residue | 81 | 1 | Phosphoserine Ref.6 Ref.3 | ||||||
| Modified residue | 85 | 1 | Phosphothreonine Ref.3 | ||||||
| Modified residue | 92 | 1 | Phosphoserine Ref.5 | ||||||
| Modified residue | 94 | 1 | Phosphothreonine Ref.6 | ||||||
| Modified residue | 347 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 351 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 365 | 1 | Phosphoserine Ref.5 Ref.6 | ||||||
| Modified residue | 1460 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 1483 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 1506 | 1 | Phosphoserine Ref.6 Ref.3 | ||||||
| Modified residue | 1545 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 1551 | 1 | Phosphothreonine Ref.6 | ||||||
| Modified residue | 1552 | 1 | Phosphoserine Ref.6 | ||||||
Sequences
| ||||||||||||||||||
References
| [1] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W.Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [2] | "A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. |
| [3] | "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81; THR-85 AND SER-1506, MASS SPECTROMETRY. |
| [4] | "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-53, MASS SPECTROMETRY. |
| [5] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-53; SER-92 AND SER-365, MASS SPECTROMETRY. |
| [6] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-53; THR-58; TYR-77; SER-81; THR-94; SER-347; SER-351; SER-365; SER-1460; SER-1483; SER-1506; SER-1545; THR-1551 AND SER-1552, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U18922 Genomic DNA. Translation: AAB64693.1. |
| PIR | S50669. |
| RefSeq | NP_011093.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-7949N. |
| IntAct | P32660. 22 interactions. |
| STRING | P32660. |
Protein family/group databases | |
| TCDB | 3.A.3.8.4. P-type ATPase (P-ATPase) superfamily. |
Proteomic databases | |
| PeptideAtlas | P32660. |
Genome annotation databases | |
| Ensembl | YER166W; YER166W; YER166W; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 856913. |
| KEGG | sce:YER166W. |
| NMPDR | fig|4932.3.peg.2179. |
Organism-specific databases | |
| CYGD | YER166w. |
| SGD | S000000968. DNF1. |
Phylogenomic databases | |
| eggNOG | fuNOG04167. |
| HOGENOM | HBG745019. |
| OMA | ALFWYGI. |
| OrthoDB | EOG9C898R. |
| PhylomeDB | P32660. |
Enzyme and pathway databases | |
| BRENDA | 3.6.3.1. 250. |
Gene expression databases | |
| ArrayExpress | P32660. |
| Genevestigator | P32660. |
| GermOnline | YER166W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR006539. ATPase_P-typ_Plipid-transl. [Graphical view] |
| PANTHER | PTHR11939. ATPase_P. 1 hit. |
| Pfam | PF00122. E1-E2_ATPase. 1 hit. [Graphical view] |
| PRINTS | PR00119. CATATPASE. |
| TIGRFAMs | TIGR01652. ATPase-Plipid. 1 hit. TIGR01494. ATPase_P-type. 3 hits. |
| PROSITE | PS00154. ATPASE_E1_E2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 983359. |
Entry information
| Entry name | ATC5_YEAST | ||||||||
| Accession | Primary (citable) accession number: P32660 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome V Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names |

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