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P32660

- ATC5_YEAST

UniProt

P32660 - ATC5_YEAST

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Protein

Phospholipid-transporting ATPase DNF1

Gene
DNF1, YER166W, SYGP-ORF7
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids Reviewed prediction.

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei667 – 66714-aspartylphosphate intermediate By similarity

GO - Molecular functioni

  1. ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Source: SGD
  2. ATP binding Source: UniProtKB-KW
  3. cation-transporting ATPase activity Source: InterPro
  4. magnesium ion binding Source: InterPro
  5. phospholipid-translocating ATPase activity Source: SGD
  6. protein binding Source: IntAct

GO - Biological processi

  1. endocytosis Source: SGD
  2. establishment or maintenance of cell polarity Source: SGD
  3. intracellular protein transport Source: SGD
  4. ion transmembrane transport Source: GOC
  5. phospholipid translocation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30327-MONOMER.
ReactomeiREACT_96459. Ion transport by P-type ATPases.

Protein family/group databases

TCDBi3.A.3.8.4. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid-transporting ATPase DNF1 (EC:3.6.3.1)
Alternative name(s):
Flippase DNF1
Gene namesi
Name:DNF1
Ordered Locus Names:YER166W
ORF Names:SYGP-ORF7
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER166w.
SGDiS000000968. DNF1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Golgi apparatustrans-Golgi network membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 214214Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei215 – 23521Helical; Reviewed predictionAdd
BLAST
Topological domaini236 – 2394Extracellular Reviewed prediction
Transmembranei240 – 26021Helical; Reviewed predictionAdd
BLAST
Topological domaini261 – 553293Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei554 – 57421Helical; Reviewed predictionAdd
BLAST
Topological domaini575 – 59420Extracellular Reviewed predictionAdd
BLAST
Transmembranei595 – 61521Helical; Reviewed predictionAdd
BLAST
Topological domaini616 – 1188573Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei1189 – 120921Helical; Reviewed predictionAdd
BLAST
Topological domaini1210 – 121910Extracellular Reviewed prediction
Transmembranei1220 – 124021Helical; Reviewed predictionAdd
BLAST
Topological domaini1241 – 127030Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei1271 – 129121Helical; Reviewed predictionAdd
BLAST
Topological domaini1292 – 130716Extracellular Reviewed predictionAdd
BLAST
Transmembranei1308 – 132821Helical; Reviewed predictionAdd
BLAST
Topological domaini1329 – 13346Cytoplasmic Reviewed prediction
Transmembranei1335 – 135521Helical; Reviewed predictionAdd
BLAST
Topological domaini1356 – 137520Extracellular Reviewed predictionAdd
BLAST
Transmembranei1376 – 139621Helical; Reviewed predictionAdd
BLAST
Topological domaini1397 – 1571175Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endosome membrane Source: UniProtKB-SubCell
  2. Golgi apparatus Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15711571Phospholipid-transporting ATPase DNF1PRO_0000046235Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 531Phosphoserine3 Publications
Modified residuei70 – 701Phosphothreonine1 Publication
Modified residuei81 – 811Phosphoserine2 Publications
Modified residuei85 – 851Phosphothreonine2 Publications
Modified residuei92 – 921Phosphoserine1 Publication
Modified residuei94 – 941Phosphothreonine1 Publication
Modified residuei104 – 1041Phosphoserine1 Publication
Modified residuei109 – 1091Phosphothreonine1 Publication
Modified residuei351 – 3511Phosphoserine1 Publication
Modified residuei365 – 3651Phosphoserine1 Publication
Modified residuei1506 – 15061Phosphoserine3 Publications
Modified residuei1551 – 15511Phosphothreonine1 Publication
Modified residuei1552 – 15521Phosphoserine1 Publication
Modified residuei1563 – 15631Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated by FPK1 and KIN82.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32660.
PaxDbiP32660.
PeptideAtlasiP32660.

Expressioni

Gene expression databases

GenevestigatoriP32660.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
LEM3P428384EBI-3121,EBI-28396

Protein-protein interaction databases

BioGridi36919. 36 interactions.
DIPiDIP-7949N.
IntActiP32660. 16 interactions.
MINTiMINT-1364907.
STRINGi4932.YER166W.

Structurei

3D structure databases

ProteinModelPortaliP32660.
SMRiP32660. Positions 1107-1174.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00740000115303.
HOGENOMiHOG000202528.
KOiK01530.
OMAiTHILEGV.
OrthoDBiEOG7FR7QR.

Family and domain databases

Gene3Di2.70.150.10. 3 hits.
3.40.1110.10. 2 hits.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR006539. ATPase_P-typ_Plipid-transp.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
IPR026871. PLip_transp_ATPase.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PTHR24092:SF7. PTHR24092:SF7. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 2 hits.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32660-1 [UniParc]FASTAAdd to Basket

« Hide

MSGTFHGDGH APMSPFEDTF QFEDNSSNED THIAPTHFDD GATSNKYSRP     50
QVSFNDETPK NKREDAEEFT FNDDTEYDNH SFQPTPKLNN GSGTFDDVEL 100
DNDSGEPHTN YDGMKRFRMG TKRNKKGNPI MGRSKTLKWA RKNIPNPFED 150
FTKDDIDPGA INRAQELRTV YYNMPLPKDM IDEEGNPIMQ YPRNKIRTTK 200
YTPLTFLPKN ILFQFHNFAN VYFLVLIILG AFQIFGVTNP GLSAVPLVVI 250
VIITAIKDAI EDSRRTVLDL EVNNTKTHIL EGVENENVST DNISLWRRFK 300
KANSRLLFKF IQYCKEHLTE EGKKKRMQRK RHELRVQKTV GTSGPRSSLD 350
SIDSYRVSAD YGRPSLDYDN LEQGAGEANI VDRSLPPRTD CKFAKNYWKG 400
VKVGDIVRIH NNDEIPADII LLSTSDTDGA CYVETKNLDG ETNLKVRQSL 450
KCTNTIRTSK DIARTKFWIE SEGPHSNLYT YQGNMKWRNL ADGEIRNEPI 500
TINNVLLRGC TLRNTKWAMG VVMFTGGDTK IMLNSGITPT KKSRISRELN 550
FSVVINFVLL FILCFVSGIA NGVYYDKKGR SRFSYEFGTI AGSAATNGFV 600
SFWVAVILYQ SLVPISLYIS VEIIKTAQAA FIYGDVLLYN AKLDYPCTPK 650
SWNISDDLGQ VEYIFSDKTG TLTQNVMEFK KCTINGVSYG RAYTEALAGL 700
RKRQGIDVET EGRREKAEIA KDRDTMIDEL RALSGNSQFY PEEVTFVSKE 750
FVRDLKGASG EVQQRCCEHF MLALALCHSV LVEANPDNPK KLDLKAQSPD 800
EAALVATARD VGFSFVGKTK KGLIIEMQGI QKEFEILNIL EFNSSRKRMS 850
CIVKIPGLNP GDEPRALLIC KGADSIIYSR LSRQSGSNSE AILEKTALHL 900
EQYATEGLRT LCIAQRELSW SEYEKWNEKY DIAAASLANR EDELEVVADS 950
IERELILLGG TAIEDRLQDG VPDCIELLAE AGIKLWVLTG DKVETAINIG 1000
FSCNLLNNEM ELLVIKTTGD DVKEFGSEPS EIVDALLSKY LKEYFNLTGS 1050
EEEIFEAKKD HEFPKGNYAI VIDGDALKLA LYGEDIRRKF LLLCKNCRAV 1100
LCCRVSPSQK AAVVKLVKDS LDVMTLAIGD GSNDVAMIQS ADVGIGIAGE 1150
EGRQAVMCSD YAIGQFRYLA RLVLVHGRWS YKRLAEMIPE FFYKNMIFAL 1200
ALFWYGIYND FDGSYLYEYT YMMFYNLAFT SLPVIFLGIL DQDVNDTISL 1250
VVPQLYRVGI LRKEWNQRKF LWYMLDGLYQ SIICFFFPYL VYHKNMIVTS 1300
NGLGLDHRYF VGVYVTTIAV ISCNTYVLLH QYRWDWFSGL FIALSCLVVF 1350
AWTGIWSSAI ASREFFKAAA RIYGAPSFWA VFFVAVLFCL LPRFTYDSFQ 1400
KFFYPTDVEI VREMWQHGHF DHYPPGYDPT DPNRPKVTKA GQHGEKIIEG 1450
IALSDNLGGS NYSRDSVVTE EIPMTFMHGE DGSPSGYQKQ ETWMTSPKET 1500
QDLLQSPQFQ QAQTFGRGPS TNVRSSLDRT REQMIATNQL DNRYSVERAR 1550
TSLDLPGVTN AASLIGTQQN N 1571
Length:1,571
Mass (Da):177,798
Last modified:February 1, 1995 - v2
Checksum:i3CC3FBA8ADDE8960
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18922 Genomic DNA. Translation: AAB64693.1.
BK006939 Genomic DNA. Translation: DAA07828.1.
PIRiS50669.
RefSeqiNP_011093.3. NM_001179056.3.

Genome annotation databases

EnsemblFungiiYER166W; YER166W; YER166W.
GeneIDi856913.
KEGGisce:YER166W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18922 Genomic DNA. Translation: AAB64693.1 .
BK006939 Genomic DNA. Translation: DAA07828.1 .
PIRi S50669.
RefSeqi NP_011093.3. NM_001179056.3.

3D structure databases

ProteinModelPortali P32660.
SMRi P32660. Positions 1107-1174.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36919. 36 interactions.
DIPi DIP-7949N.
IntActi P32660. 16 interactions.
MINTi MINT-1364907.
STRINGi 4932.YER166W.

Protein family/group databases

TCDBi 3.A.3.8.4. the p-type atpase (p-atpase) superfamily.

Proteomic databases

MaxQBi P32660.
PaxDbi P32660.
PeptideAtlasi P32660.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER166W ; YER166W ; YER166W .
GeneIDi 856913.
KEGGi sce:YER166W.

Organism-specific databases

CYGDi YER166w.
SGDi S000000968. DNF1.

Phylogenomic databases

eggNOGi COG0474.
GeneTreei ENSGT00740000115303.
HOGENOMi HOG000202528.
KOi K01530.
OMAi THILEGV.
OrthoDBi EOG7FR7QR.

Enzyme and pathway databases

BioCyci YEAST:G3O-30327-MONOMER.
Reactomei REACT_96459. Ion transport by P-type ATPases.

Miscellaneous databases

NextBioi 983359.

Gene expression databases

Genevestigatori P32660.

Family and domain databases

Gene3Di 2.70.150.10. 3 hits.
3.40.1110.10. 2 hits.
3.40.50.1000. 2 hits.
InterProi IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR006539. ATPase_P-typ_Plipid-transp.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
IPR026871. PLip_transp_ATPase.
[Graphical view ]
PANTHERi PTHR24092. PTHR24092. 1 hit.
PTHR24092:SF7. PTHR24092:SF7. 1 hit.
Pfami PF00122. E1-E2_ATPase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SUPFAMi SSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 2 hits.
TIGRFAMsi TIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  4. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81; THR-85 AND SER-1506, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  5. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-53, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Protein kinases Fpk1p and Fpk2p are novel regulators of phospholipid asymmetry."
    Nakano K., Yamamoto T., Kishimoto T., Noji T., Tanaka K.
    Mol. Biol. Cell 19:1783-1797(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION BY FPK1 AND KIN82.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-53; SER-351 AND SER-1506, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-53; THR-70; SER-81; THR-85; SER-92; THR-94; SER-104; THR-109; SER-365; SER-1506; THR-1551; SER-1552 AND SER-1563, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiATC5_YEAST
AccessioniPrimary (citable) accession number: P32660
Secondary accession number(s): D3DM74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1995
Last modified: September 3, 2014
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi