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Protein

Glutathione-specific gamma-glutamylcyclotransferase

Gene

GCG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage glutathione into 5-oxoproline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Allows utilization of gluthathione through subsequent cleavage of the Cys-Gly dipeptide by Cys-Gly metallodipeptidase DUG1.1 Publication

Catalytic activityi

Glutathione = 5-oxoproline + cysteinylglycine.1 Publication

Kineticsi

  1. KM=1.52 mM for glutathione1 Publication
  1. Vmax=110 µmol/h/mg enzyme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei115Proton acceptorBy similarity1

GO - Molecular functioni

  • gamma-glutamylcyclotransferase activity Source: SGD

GO - Biological processi

  • glutathione catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-30324-MONOMER.
ReactomeiR-SCE-174403. Glutathione synthesis and recycling.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione-specific gamma-glutamylcyclotransferase1 Publication (EC:2.3.2.-1 Publication)
Short name:
Gamma-GCG1 Publication
Gene namesi
Name:GCG11 Publication
Ordered Locus Names:YER163CImported
ORF Names:SYGP-ORF3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER163C.
SGDiS000000965. GCG1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi115E → Q: Loss of catalytic activity against glutathione. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002026571 – 232Glutathione-specific gamma-glutamylcyclotransferaseAdd BLAST232

Proteomic databases

MaxQBiP32656.
PRIDEiP32656.

Interactioni

Protein-protein interaction databases

BioGridi36916. 20 interactors.
DIPiDIP-4233N.
IntActiP32656. 1 interactor.
MINTiMINT-510594.

Structurei

3D structure databases

ProteinModelPortaliP32656.
SMRiP32656.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni10 – 15Substrate bindingBy similarity6

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000003855.
HOGENOMiHOG000264264.
InParanoidiP32656.
KOiK07232.
OMAiSHDSHLF.
OrthoDBiEOG092C4Q9H.

Family and domain databases

CDDicd06661. GGCT_like. 1 hit.
Gene3Di3.10.490.10. 1 hit.
InterProiIPR006840. ChaC.
IPR013024. GGCT-like.
[Graphical view]
PANTHERiPTHR12192. PTHR12192. 2 hits.
PfamiPF04752. ChaC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32656-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNDNSGIWV LGYGSLIYKP PSHYTHRIPA IIHGFARRFW QSSTDHRGTP
60 70 80 90 100
ANPGRVATLI PYEDIIRQTA FLKNVNLYSE SAPIQDPDDL VTIGVVYYIP
110 120 130 140 150
PEHAQEVREY LNVREQNGYT LHEVEVHLET NREHEAELGE ALEQLPRHNK
160 170 180 190 200
SGKRVLLTSV YIGTIDNEAF VGPETVDETA KVIAVSHGPS GSNYEYLAKL
210 220 230
EQALAQMPIM KERGRITDHY LTALLETVNK YR
Length:232
Mass (Da):26,294
Last modified:October 1, 1993 - v1
Checksum:i6B0B5306F348A59D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18917 Genomic DNA. Translation: AAB64690.1.
AY692652 Genomic DNA. Translation: AAT92671.1.
BK006939 Genomic DNA. Translation: DAA07825.1.
PIRiS30817.
RefSeqiNP_011090.3. NM_001179053.3.

Genome annotation databases

EnsemblFungiiYER163C; YER163C; YER163C.
GeneIDi856910.
KEGGisce:YER163C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18917 Genomic DNA. Translation: AAB64690.1.
AY692652 Genomic DNA. Translation: AAT92671.1.
BK006939 Genomic DNA. Translation: DAA07825.1.
PIRiS30817.
RefSeqiNP_011090.3. NM_001179053.3.

3D structure databases

ProteinModelPortaliP32656.
SMRiP32656.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36916. 20 interactors.
DIPiDIP-4233N.
IntActiP32656. 1 interactor.
MINTiMINT-510594.

Proteomic databases

MaxQBiP32656.
PRIDEiP32656.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER163C; YER163C; YER163C.
GeneIDi856910.
KEGGisce:YER163C.

Organism-specific databases

EuPathDBiFungiDB:YER163C.
SGDiS000000965. GCG1.

Phylogenomic databases

GeneTreeiENSGT00390000003855.
HOGENOMiHOG000264264.
InParanoidiP32656.
KOiK07232.
OMAiSHDSHLF.
OrthoDBiEOG092C4Q9H.

Enzyme and pathway databases

BioCyciYEAST:G3O-30324-MONOMER.
ReactomeiR-SCE-174403. Glutathione synthesis and recycling.

Miscellaneous databases

PROiP32656.

Family and domain databases

CDDicd06661. GGCT_like. 1 hit.
Gene3Di3.10.490.10. 1 hit.
InterProiIPR006840. ChaC.
IPR013024. GGCT-like.
[Graphical view]
PANTHERiPTHR12192. PTHR12192. 2 hits.
PfamiPF04752. ChaC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHAC_YEAST
AccessioniPrimary (citable) accession number: P32656
Secondary accession number(s): D3DM71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.