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Protein

Glutathione-specific gamma-glutamylcyclotransferase

Gene

GCG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage glutathione into 5-oxoproline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Allows utilization of gluthathione through subsequent cleavage of the Cys-Gly dipeptide by Cys-Gly metallodipeptidase DUG1.1 Publication

Miscellaneous

Present with 1300 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Glutathione = 5-oxoproline + cysteinylglycine.1 Publication

Kineticsi

  1. KM=1.52 mM for glutathione1 Publication
  1. Vmax=110 µmol/h/mg enzyme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei115Proton acceptorBy similarity1

GO - Molecular functioni

  • gamma-glutamylcyclotransferase activity Source: SGD

GO - Biological processi

  • glutathione catabolic process Source: SGD

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-30324-MONOMER.
ReactomeiR-SCE-174403. Glutathione synthesis and recycling.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione-specific gamma-glutamylcyclotransferase1 Publication (EC:2.3.2.-1 Publication)
Short name:
Gamma-GCG1 Publication
Gene namesi
Name:GCG11 Publication
Ordered Locus Names:YER163CImported
ORF Names:SYGP-ORF3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER163C.
SGDiS000000965. GCG1.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi115E → Q: Loss of catalytic activity against glutathione. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002026571 – 232Glutathione-specific gamma-glutamylcyclotransferaseAdd BLAST232

Proteomic databases

MaxQBiP32656.
PRIDEiP32656.

Interactioni

Protein-protein interaction databases

BioGridi36916. 93 interactors.
DIPiDIP-4233N.
IntActiP32656. 1 interactor.
MINTiMINT-510594.
STRINGi4932.YER163C.

Structurei

Secondary structure

1232
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 14Combined sources7
Helixi15 – 18Combined sources4
Beta strandi25 – 48Combined sources24
Beta strandi50 – 52Combined sources3
Beta strandi54 – 61Combined sources8
Helixi62 – 65Combined sources4
Helixi69 – 78Combined sources10
Helixi87 – 89Combined sources3
Beta strandi91 – 99Combined sources9
Helixi101 – 113Combined sources13
Turni115 – 118Combined sources4
Beta strandi119 – 126Combined sources8
Helixi132 – 134Combined sources3
Helixi135 – 144Combined sources10
Turni149 – 151Combined sources3
Beta strandi154 – 163Combined sources10
Helixi176 – 185Combined sources10
Helixi193 – 205Combined sources13
Helixi219 – 232Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5HWIX-ray1.75A1-232[»]
5HWKX-ray1.34A/B1-232[»]
ProteinModelPortaliP32656.
SMRiP32656.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni10 – 15Substrate bindingBy similarity6

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000003855.
HOGENOMiHOG000264264.
InParanoidiP32656.
KOiK07232.
OMAiDFPYEEK.
OrthoDBiEOG092C4Q9H.

Family and domain databases

CDDicd06661. GGCT_like. 1 hit.
InterProiView protein in InterPro
IPR006840. ChaC.
IPR013024. GGCT-like.
PANTHERiPTHR12192. PTHR12192. 1 hit.
PfamiView protein in Pfam
PF04752. ChaC. 1 hit.

Sequencei

Sequence statusi: Complete.

P32656-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNDNSGIWV LGYGSLIYKP PSHYTHRIPA IIHGFARRFW QSSTDHRGTP
60 70 80 90 100
ANPGRVATLI PYEDIIRQTA FLKNVNLYSE SAPIQDPDDL VTIGVVYYIP
110 120 130 140 150
PEHAQEVREY LNVREQNGYT LHEVEVHLET NREHEAELGE ALEQLPRHNK
160 170 180 190 200
SGKRVLLTSV YIGTIDNEAF VGPETVDETA KVIAVSHGPS GSNYEYLAKL
210 220 230
EQALAQMPIM KERGRITDHY LTALLETVNK YR
Length:232
Mass (Da):26,294
Last modified:October 1, 1993 - v1
Checksum:i6B0B5306F348A59D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18917 Genomic DNA. Translation: AAB64690.1.
AY692652 Genomic DNA. Translation: AAT92671.1.
BK006939 Genomic DNA. Translation: DAA07825.1.
PIRiS30817.
RefSeqiNP_011090.3. NM_001179053.3.

Genome annotation databases

EnsemblFungiiYER163C; YER163C; YER163C.
GeneIDi856910.
KEGGisce:YER163C.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCHAC_YEAST
AccessioniPrimary (citable) accession number: P32656
Secondary accession number(s): D3DM71
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 5, 2017
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names