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Protein

Glutathione-specific gamma-glutamylcyclotransferase

Gene

GCG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Allows utilization of gluthathione through subsequent cleavage of the Cys-Gly dipeptide by Cys-Gly metallodipeptidase DUG1.1 Publication

Miscellaneous

Present with 1300 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Glutathione = L-cysteinylglycine + 5-oxo-L-proline.1 Publication

Kineticsi

kcat is 50.8 min(-1) for glutathione.1 Publication
  1. KM=1.52 mM for glutathione1 Publication
  1. Vmax=110 µmol/h/mg enzyme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei115Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • glutathione catabolic process Source: SGD

Keywordsi

Molecular functionLyase

Enzyme and pathway databases

BioCyciMetaCyc:G3O-30324-MONOMER
YEAST:G3O-30324-MONOMER
ReactomeiR-SCE-174403 Glutathione synthesis and recycling

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione-specific gamma-glutamylcyclotransferase1 Publication (EC:4.3.2.71 Publication)
Short name:
Gamma-GCG1 Publication
Gene namesi
Name:GCG11 Publication
Ordered Locus Names:YER163CImported
ORF Names:SYGP-ORF3
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi

Organism-specific databases

EuPathDBiFungiDB:YER163C
SGDiS000000965 GCG1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi115E → Q: Loss of catalytic activity against glutathione. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002026571 – 232Glutathione-specific gamma-glutamylcyclotransferaseAdd BLAST232

Proteomic databases

MaxQBiP32656
PaxDbiP32656
PRIDEiP32656

Interactioni

Protein-protein interaction databases

BioGridi36916, 95 interactors
DIPiDIP-4233N
IntActiP32656, 1 interactor
STRINGi4932.YER163C

Structurei

Secondary structure

1232
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 14Combined sources7
Helixi15 – 18Combined sources4
Beta strandi25 – 48Combined sources24
Beta strandi50 – 52Combined sources3
Beta strandi54 – 61Combined sources8
Helixi62 – 65Combined sources4
Helixi69 – 78Combined sources10
Helixi87 – 89Combined sources3
Beta strandi91 – 99Combined sources9
Helixi101 – 113Combined sources13
Turni115 – 118Combined sources4
Beta strandi119 – 126Combined sources8
Helixi132 – 134Combined sources3
Helixi135 – 144Combined sources10
Turni149 – 151Combined sources3
Beta strandi154 – 163Combined sources10
Helixi176 – 185Combined sources10
Helixi193 – 205Combined sources13
Helixi219 – 232Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5HWIX-ray1.75A1-232[»]
5HWKX-ray1.34A/B1-232[»]
ProteinModelPortaliP32656
SMRiP32656
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni10 – 15Substrate bindingBy similarity6

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000003855
HOGENOMiHOG000264264
InParanoidiP32656
KOiK07232
OMAiDFPYEEK
OrthoDBiEOG092C4Q9H

Family and domain databases

CDDicd06661 GGCT_like, 1 hit
InterProiView protein in InterPro
IPR006840 ChaC
IPR013024 GGCT-like
PANTHERiPTHR12192 PTHR12192, 1 hit
PfamiView protein in Pfam
PF04752 ChaC, 1 hit

Sequencei

Sequence statusi: Complete.

P32656-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNDNSGIWV LGYGSLIYKP PSHYTHRIPA IIHGFARRFW QSSTDHRGTP
60 70 80 90 100
ANPGRVATLI PYEDIIRQTA FLKNVNLYSE SAPIQDPDDL VTIGVVYYIP
110 120 130 140 150
PEHAQEVREY LNVREQNGYT LHEVEVHLET NREHEAELGE ALEQLPRHNK
160 170 180 190 200
SGKRVLLTSV YIGTIDNEAF VGPETVDETA KVIAVSHGPS GSNYEYLAKL
210 220 230
EQALAQMPIM KERGRITDHY LTALLETVNK YR
Length:232
Mass (Da):26,294
Last modified:October 1, 1993 - v1
Checksum:i6B0B5306F348A59D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18917 Genomic DNA Translation: AAB64690.1
AY692652 Genomic DNA Translation: AAT92671.1
BK006939 Genomic DNA Translation: DAA07825.1
PIRiS30817
RefSeqiNP_011090.3, NM_001179053.3

Genome annotation databases

EnsemblFungiiYER163C; YER163C; YER163C
GeneIDi856910
KEGGisce:YER163C

Similar proteinsi

Entry informationi

Entry nameiCHAC_YEAST
AccessioniPrimary (citable) accession number: P32656
Secondary accession number(s): D3DM71
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

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