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Protein

Putative ATP-dependent RNA helicase ECM32

Gene

ECM32

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable RNA helicase, which may be involved in modulation of the translation termination process. Probably unwinds double-stranded RNA. In vitro, unwinds covalently closed, circular DNA in the presence of a DNA topoisomerase TOP1 and replication factor-A protein RFA1.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi670 – 677ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB-KW
  • DNA helicase activity Source: SGD
  • RNA binding Source: UniProtKB-KW
  • RNA helicase activity Source: SGD

GO - Biological processi

  • regulation of translational termination Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30335-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative ATP-dependent RNA helicase ECM32 (EC:3.6.4.13)
Alternative name(s):
DNA helicase B
Short name:
Hcs B
DNA helicase III
Extracellular mutant protein 32
Helicase 1
Short name:
scHelI
Modulator of translation termination protein 1
Gene namesi
Name:ECM32
Synonyms:HEL1, MTT1
Ordered Locus Names:YER176W
ORF Names:SYGP-ORF61
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER176W.
SGDiS000000978. ECM32.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic stress granule Source: SGD
  • polysome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Sensitivity to drugs that affect translation fidelity. Overexpression results in a nonsense suppression phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000807251 – 1121Putative ATP-dependent RNA helicase ECM32Add BLAST1121

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei227PhosphoserineCombined sources1
Modified residuei392PhosphoserineCombined sources1
Modified residuei465PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32644.
PRIDEiP32644.

PTM databases

iPTMnetiP32644.

Interactioni

Subunit structurei

Interacts with the peptidyl release factors SUP35 and weakly with SUP45.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SUP35P054533EBI-22223,EBI-6540

Protein-protein interaction databases

BioGridi36929. 17 interactors.
DIPiDIP-5404N.
IntActiP32644. 3 interactors.
MINTiMINT-499764.

Structurei

3D structure databases

ProteinModelPortaliP32644.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA2/NAM7 helicase family.Curated

Phylogenomic databases

GeneTreeiENSGT00860000134985.
HOGENOMiHOG000094475.
InParanoidiP32644.
OMAiSICLHHK.
OrthoDBiEOG092C0D29.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

P32644-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFQCRTCSQ AYDAEQMMKH LSSTRHKTVF DTSNDEDICC EECQDKNIHQ
60 70 80 90 100
LQIIRFGGED MVLLCNSCFR KEYSETERPS TSYSLQNGSI LKFWEKYVKV
110 120 130 140 150
RECCCDECGE ESNLNANRNG EVLCDKCLPK SNRAKDFVSE KSGRFLYIYL
160 170 180 190 200
GLNETQNSTR KPRKKGGRRV GRGKKGRKGA KIKKEKKETF EAKISRIAYE
210 220 230 240 250
VKKENSTIQS SSSSNLRNFK GFKAVESDPV VAAKVSKSET SRSNPGPSNR
260 270 280 290 300
NKGKGNKANH KKNSGNGIGK EKERKTNIRN NVRNSQPIPE DRKNTNSHVT
310 320 330 340 350
TNSGGKGKNE SVDKHQLPQP KALNGNGSGS TNTTGLKKGK KDHAGQKTKG
360 370 380 390 400
NDKTGNKNPR EAKLNSAGRK NALGKKSNNQ PNKGTSRWTI GSDTESSREP
410 420 430 440 450
SISPNENTTS ITKSRNRNKK ASKPTLNEKS KTTTMPKKLE TKNQEKNNGK
460 470 480 490 500
TKDGKLIYEE GEPLTRYNTF KSTLSYPDLN TYLNDYSFAL FLEQKLENEF
510 520 530 540 550
VQNFNILWPR NEKDTAFIIN VEKNNNSELE KLLPANLLAL GRPAFNERQP
560 570 580 590 600
FFFCTQDEQK VWYIFIKELS IQRGKYVLLV ELFSWNNLSL PTKNGSSQFK
610 620 630 640 650
LLPTSAQTSR ILFAMTRITN PKFIDLLLGQ KPIKEIYFDN RLKFSSDKLN
660 670 680 690 700
RSQKTAVEHV LNNSITILQG PPGTGKTSTI EEIIIQVIER FHAFPILCVA
710 720 730 740 750
ASNIAIDNIA EKIMENRPQI KILRILSKKK EQQYSDDHPL GEICLHNIVY
760 770 780 790 800
KNLSPDMQVV ANKTRRGEMI SKSEDTKFYK EKNRVTNKVV SQSQIIFTTN
810 820 830 840 850
IAAGGRELKV IKECPVVIMD EATQSSEAST LVPLSLPGIR NFVFVGDEKQ
860 870 880 890 900
LSSFSNIPQL ETSLFERVLS NGTYKNPLML DTQYRMHPKI SEFPIKKIYN
910 920 930 940 950
GELKDGVTDE QKAWPGVQHP LFFYQCDLGP ESRVRSTQRD IVGFTYENKH
960 970 980 990 1000
ECVEIVKIIQ ILMLDKKVPL EEIGVITPYS AQRDLLSDIL TKNVVINPKQ
1010 1020 1030 1040 1050
ISMQQEYDEI ELFNAAGSQG TAGSLQNNVI NIINGLHVAT VDSFQGHEKS
1060 1070 1080 1090 1100
FIIFSCVRNN TENKIGFLRD KRRLNVALTR AKHGLIVVGN KNVLRKGDPL
1110 1120
WKDYITYLEE QEVIFTDLTA Y
Length:1,121
Mass (Da):126,970
Last modified:October 1, 1993 - v1
Checksum:i641C4AA6810282A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18922 Genomic DNA. Translation: AAB64703.1.
BK006939 Genomic DNA. Translation: DAA07839.1.
PIRiS30862.
RefSeqiNP_011103.1. NM_001179066.1.

Genome annotation databases

EnsemblFungiiYER176W; YER176W; YER176W.
GeneIDi856923.
KEGGisce:YER176W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18922 Genomic DNA. Translation: AAB64703.1.
BK006939 Genomic DNA. Translation: DAA07839.1.
PIRiS30862.
RefSeqiNP_011103.1. NM_001179066.1.

3D structure databases

ProteinModelPortaliP32644.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36929. 17 interactors.
DIPiDIP-5404N.
IntActiP32644. 3 interactors.
MINTiMINT-499764.

PTM databases

iPTMnetiP32644.

Proteomic databases

MaxQBiP32644.
PRIDEiP32644.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER176W; YER176W; YER176W.
GeneIDi856923.
KEGGisce:YER176W.

Organism-specific databases

EuPathDBiFungiDB:YER176W.
SGDiS000000978. ECM32.

Phylogenomic databases

GeneTreeiENSGT00860000134985.
HOGENOMiHOG000094475.
InParanoidiP32644.
OMAiSICLHHK.
OrthoDBiEOG092C0D29.

Enzyme and pathway databases

BioCyciYEAST:G3O-30335-MONOMER.

Miscellaneous databases

PROiP32644.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiECM32_YEAST
AccessioniPrimary (citable) accession number: P32644
Secondary accession number(s): D3DM85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 30, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 736 molecules/cell in log phase SD medium.1 Publication

Caution

ECM32 was originally identified as a DNA helicase and as component of DNA polymerase I.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.