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Protein

Monothiol glutaredoxin-4

Gene

GRX4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters (By similarity). Binds one iron-sulfur cluster per dimer. The iron-sulfur cluster is bound between subunits, and is complexed by a bound glutathione and a cysteine residue from each subunit (Probable).By similarityCurated1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei163GlutathioneBy similarity1
Metal bindingi171Iron-sulfur (2Fe-2S); shared with dimeric partnerBy similarity1
Binding sitei200GlutathioneBy similarity1
Binding sitei212Glutathione; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: SGD
  • cell redox homeostasis Source: InterPro
  • cellular iron ion homeostasis Source: SGD
  • cellular response to oxidative stress Source: SGD
  • negative regulation of transcription regulatory region DNA binding Source: SGD
  • positive regulation of histone deacetylation Source: SGD
Complete GO annotation...

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30334-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Monothiol glutaredoxin-4
Gene namesi
Name:GRX4
Ordered Locus Names:YER174C
ORF Names:SYGP-ORF64
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER174C.
SGDiS000000976. GRX4.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022501 – 244Monothiol glutaredoxin-4Add BLAST244

Proteomic databases

MaxQBiP32642.
PRIDEiP32642.

PTM databases

iPTMnetiP32642.

Interactioni

Subunit structurei

Homodimer. Heterodimer with FRA2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AFT1P221492EBI-22211,EBI-2332
BUD32P5332310EBI-22211,EBI-3809

GO - Molecular functioni

  • RNA polymerase II activating transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi36927. 35 interactors.
DIPiDIP-6635N.
IntActiP32642. 13 interactors.
MINTiMINT-696308.

Structurei

3D structure databases

ProteinModelPortaliP32642.
SMRiP32642.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 110ThioredoxinPROSITE-ProRule annotationAdd BLAST108
Domaini146 – 244GlutaredoxinPROSITE-ProRule annotationAdd BLAST99

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni225 – 226Glutathione bindingBy similarity2

Sequence similaritiesi

Contains 1 glutaredoxin domain.PROSITE-ProRule annotation
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center

Phylogenomic databases

GeneTreeiENSGT00550000075030.
HOGENOMiHOG000165751.
InParanoidiP32642.
OMAiNNWPTFP.
OrthoDBiEOG092C3TIP.

Family and domain databases

CDDicd03028. GRX_PICOT_like. 1 hit.
Gene3Di3.40.30.10. 2 hits.
InterProiIPR002109. Glutaredoxin.
IPR033658. GRX_PICOT-like.
IPR004480. Monothiol_GRX-rel.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PANTHERiPTHR10293. PTHR10293. 1 hit.
PfamiPF00462. Glutaredoxin. 1 hit.
PF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 2 hits.
PROSITEiPS51354. GLUTAREDOXIN_2. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32642-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVVEIKSQD QFTQLTTTNA ANKLIVLYFK AQWADPCKTM SQVLEAVSEK
60 70 80 90 100
VRQEDVRFLS IDADEHPEIS DLFEIAAVPY FVFIQNGTIV KEISAADPKE
110 120 130 140 150
FVKSLEILSN ASASLANNAK GPKSTSDEES SGSSDDEEDE TEEEINARLV
160 170 180 190 200
KLVQAAPVML FMKGSPSEPK CGFSRQLVGI LREHQIRFGF FDILRDENVR
210 220 230 240
QSLKKFSDWP TFPQLYINGE FQGGLDIIKE SIEEDPEYFQ HALQ
Length:244
Mass (Da):27,493
Last modified:October 1, 1993 - v1
Checksum:i86B4C4993545B5B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18922 Genomic DNA. Translation: AAB64701.1.
BK006939 Genomic DNA. Translation: DAA07836.1.
PIRiS30860.
RefSeqiNP_011101.3. NM_001179064.3.

Genome annotation databases

EnsemblFungiiYER174C; YER174C; YER174C.
GeneIDi856921.
KEGGisce:YER174C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18922 Genomic DNA. Translation: AAB64701.1.
BK006939 Genomic DNA. Translation: DAA07836.1.
PIRiS30860.
RefSeqiNP_011101.3. NM_001179064.3.

3D structure databases

ProteinModelPortaliP32642.
SMRiP32642.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36927. 35 interactors.
DIPiDIP-6635N.
IntActiP32642. 13 interactors.
MINTiMINT-696308.

PTM databases

iPTMnetiP32642.

Proteomic databases

MaxQBiP32642.
PRIDEiP32642.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER174C; YER174C; YER174C.
GeneIDi856921.
KEGGisce:YER174C.

Organism-specific databases

EuPathDBiFungiDB:YER174C.
SGDiS000000976. GRX4.

Phylogenomic databases

GeneTreeiENSGT00550000075030.
HOGENOMiHOG000165751.
InParanoidiP32642.
OMAiNNWPTFP.
OrthoDBiEOG092C3TIP.

Enzyme and pathway databases

BioCyciYEAST:G3O-30334-MONOMER.

Miscellaneous databases

PROiP32642.

Family and domain databases

CDDicd03028. GRX_PICOT_like. 1 hit.
Gene3Di3.40.30.10. 2 hits.
InterProiIPR002109. Glutaredoxin.
IPR033658. GRX_PICOT-like.
IPR004480. Monothiol_GRX-rel.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PANTHERiPTHR10293. PTHR10293. 1 hit.
PfamiPF00462. Glutaredoxin. 1 hit.
PF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 2 hits.
PROSITEiPS51354. GLUTAREDOXIN_2. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLRX4_YEAST
AccessioniPrimary (citable) accession number: P32642
Secondary accession number(s): D3DM82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 30, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7800 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.