Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Monothiol glutaredoxin-4

Gene

GRX4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters (By similarity). Binds one iron-sulfur cluster per dimer. The iron-sulfur cluster is bound between subunits, and is complexed by a bound glutathione and a cysteine residue from each subunit (Probable).By similarityCurated1 Publication

Miscellaneous

Present with 7800 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei163GlutathioneBy similarity1
Metal bindingi171Iron-sulfur (2Fe-2S); shared with dimeric partnerBy similarity1
Binding sitei200GlutathioneBy similarity1
Binding sitei212Glutathione; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: SGD
  • cell redox homeostasis Source: InterPro
  • cellular iron ion homeostasis Source: SGD
  • cellular response to oxidative stress Source: SGD
  • negative regulation of transcription regulatory region DNA binding Source: SGD
  • positive regulation of histone deacetylation Source: SGD

Keywordsi

Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30334-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Monothiol glutaredoxin-4
Gene namesi
Name:GRX4
Ordered Locus Names:YER174C
ORF Names:SYGP-ORF64
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER174C
SGDiS000000976 GRX4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001022501 – 244Monothiol glutaredoxin-4Add BLAST244

Proteomic databases

MaxQBiP32642
PaxDbiP32642
PRIDEiP32642

PTM databases

iPTMnetiP32642

Interactioni

Subunit structurei

Homodimer. Heterodimer with FRA2.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • RNA polymerase II activating transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi36927, 104 interactors
DIPiDIP-6635N
IntActiP32642, 15 interactors
MINTiP32642
STRINGi4932.YER174C

Structurei

3D structure databases

ProteinModelPortaliP32642
SMRiP32642
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 110ThioredoxinPROSITE-ProRule annotationAdd BLAST108
Domaini146 – 244GlutaredoxinPROSITE-ProRule annotationAdd BLAST99

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni225 – 226Glutathione bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

GeneTreeiENSGT00550000075030
HOGENOMiHOG000165751
InParanoidiP32642
OMAiNNWPTFP
OrthoDBiEOG092C3TIP

Family and domain databases

CDDicd03028 GRX_PICOT_like, 1 hit
InterProiView protein in InterPro
IPR002109 Glutaredoxin
IPR033658 GRX_PICOT-like
IPR004480 Monothiol_GRX-rel
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PANTHERiPTHR10293 PTHR10293, 1 hit
PfamiView protein in Pfam
PF00462 Glutaredoxin, 1 hit
PF00085 Thioredoxin, 1 hit
SUPFAMiSSF52833 SSF52833, 2 hits
PROSITEiView protein in PROSITE
PS51354 GLUTAREDOXIN_2, 1 hit
PS51352 THIOREDOXIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

P32642-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVVEIKSQD QFTQLTTTNA ANKLIVLYFK AQWADPCKTM SQVLEAVSEK
60 70 80 90 100
VRQEDVRFLS IDADEHPEIS DLFEIAAVPY FVFIQNGTIV KEISAADPKE
110 120 130 140 150
FVKSLEILSN ASASLANNAK GPKSTSDEES SGSSDDEEDE TEEEINARLV
160 170 180 190 200
KLVQAAPVML FMKGSPSEPK CGFSRQLVGI LREHQIRFGF FDILRDENVR
210 220 230 240
QSLKKFSDWP TFPQLYINGE FQGGLDIIKE SIEEDPEYFQ HALQ
Length:244
Mass (Da):27,493
Last modified:October 1, 1993 - v1
Checksum:i86B4C4993545B5B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18922 Genomic DNA Translation: AAB64701.1
BK006939 Genomic DNA Translation: DAA07836.1
PIRiS30860
RefSeqiNP_011101.3, NM_001179064.3

Genome annotation databases

EnsemblFungiiYER174C; YER174C; YER174C
GeneIDi856921
KEGGisce:YER174C

Similar proteinsi

Entry informationi

Entry nameiGLRX4_YEAST
AccessioniPrimary (citable) accession number: P32642
Secondary accession number(s): D3DM82
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: March 28, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health