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P32634 (PMD1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Negative regulator of sporulation PMD1
Gene names
Name:PMD1
Ordered Locus Names:YER132C
ORF Names:SYGP-ORF50
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1753 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Negatively regulates early sporulation-specific genes. Seems to exert its function by positively regulating the Ras/cAMP pathway. Required for growth under alkaline conditions. Acts synergetically with MDS3. Ref.4 Ref.10

Subcellular location

Cytoplasm Ref.5.

Miscellaneous

Present with 815 molecules/cell in log phase SD medium.

Sequence similarities

Contains 2 Kelch repeats.

Ontologies

Keywords
   Biological processMeiosis
Sporulation
   Cellular componentCytoplasm
   DomainKelch repeat
Repeat
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmeiotic nuclear division

Inferred from electronic annotation. Source: UniProtKB-KW

sporulation resulting in formation of a cellular spore

Inferred from mutant phenotype Ref.3. Source: SGD

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 17531753Negative regulator of sporulation PMD1
PRO_0000119141

Regions

Repeat143 – 19856Kelch 1
Repeat206 – 25348Kelch 2

Amino acid modifications

Modified residue2981Phosphothreonine Ref.11
Modified residue8381Phosphoserine Ref.11
Modified residue12891Phosphoserine Ref.11
Modified residue13071Phosphoserine Ref.8 Ref.11
Modified residue13561Phosphoserine Ref.8
Modified residue16641Phosphoserine Ref.7 Ref.9 Ref.11

Sequences

Sequence LengthMass (Da)Tools
P32634 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: E4252998C6C2508B

FASTA1,753195,383
        10         20         30         40         50         60 
MTVLQPPSSV CYPLNLPIVP NPNLDEATRK KLTLECRTGA AVELARSGVF VHGGLTLPLN 

        70         80         90        100        110        120 
LTIINSLQLQ KELILYFGKQ KDRNADFKTL ADWISPEIFF LDLISRTWQR INTTIDTTSE 

       130        140        150        160        170        180 
NELNNGLSFK ERLFHSMCFT ESNIYIFGGL MVSPHNGYEL IATNELWKLD LKTKCWSLIS 

       190        200        210        220        230        240 
ENPQITRRFN HSMHVLNENN ENQDTKLIIV GGLDNMDIPV KKIDIFNLRT SLWESESKSD 

       250        260        270        280        290        300 
ENPASKGSSK ILVNIDGMPI SLSHDSNFSV LIENNQAEIP TLALYYPQRE ANTSRRGTDD 

       310        320        330        340        350        360 
GSFSTYAHDL DDKSKLPKHH HHHHGDLKYF ESDDADENAV KTLMSPIVIL PLLGNSQGAR 

       370        380        390        400        410        420 
MTSNPTQNNK ENSILQVPFH LQYPSGNYFN YNIVVIGFYP DPQPSNLHCF IYNIASGKWI 

       430        440        450        460        470        480 
RVNIACTECS ISMHRFWKLL IWKSHHQALL LGTRTDDFCS PSVQKFDHIL SFSLPMLNGY 

       490        500        510        520        530        540 
NKLVNTKHTR TNNGIANSHN LNVNLSLYDH LPYSNSSTIE HTNPYTVTQG YSLDDSGIPR 

       550        560        570        580        590        600 
LTSTATSQFE NYSRYITVPL EMESTSSIFP PYAMVLGKDA LEIFGKTLSD FEFITADGDS 

       610        620        630        640        650        660 
IGVPVYLLRK RWGRYFDSLL SNGYANTSFN YEFNGDTSNI ISFSPHTASK TTKFGNSSQS 

       670        680        690        700        710        720 
SNGSLEKYFS KNGNSKSNSN TSLKKPHSVD FTSSTSSPKQ RAISHNKLSP SEPILCADEE 

       730        740        750        760        770        780 
DSRSNTLKQH ATGDTGLKET GTSNKRPIST TCSSTGMVFR VPFQDMKNSK LGLSEQSGRS 

       790        800        810        820        830        840 
TRASSVSPPP VYKKSTNDGN DSNCTLSNTP LVYRRASTVG TTTNSSVDDG FSSIRRASHP 

       850        860        870        880        890        900 
LQSYIIAKSS PSSISKASPA EKAFSRRKSS ALRFIASPNQ SRQTSFASTA STASVVSSTS 

       910        920        930        940        950        960 
GRRRNSNQIS HLGSSASLPN SPILPVLNIP LPPQEKIPLE PLPPVPKAPS RRSSSLAEYV 

       970        980        990       1000       1010       1020 
QFGRDSPVAS RRSSHSTRKS SSSDARRISN SSLLRNTLDS QLLSNSYGSD IPYEASIQEY 

      1030       1040       1050       1060       1070       1080 
GMNNGRDEEE DGDNQDYGCI SPSNIRPIFS TINAININGN FKEGEFFSSK SYINNEKSRR 

      1090       1100       1110       1120       1130       1140 
LSYISNPESV ESTNSNNNAI IELEPLLTPR SLYMPWSTAS VRAFAEFFYT AQINGKWLLA 

      1150       1160       1170       1180       1190       1200 
PVTLDLLIMA KIYEIPILYE LITEVLYKII SKKEEGLSVT CEALLNLFQQ KVSRYCNENE 

      1210       1220       1230       1240       1250       1260 
GKIRKQLDSS ESYQDTLEIK RSLANIDNGY VDSYLLRNTS MAQSIHYTDD SNGETEIDMH 

      1270       1280       1290       1300       1310       1320 
HTGISSIGSL ANRAVPTVFA GGPRDSHNSI GSIAFPSNSG VQNIRRSVSL FSPATKKKSS 

      1330       1340       1350       1360       1370       1380 
LSRETDPLDT SDQFTDDVPD SGPVSRQQNF PRRSSSFTET VPTEPTRYNY QNLDSSKSNR 

      1390       1400       1410       1420       1430       1440 
ASDDKEEQNE QATLQDISNF DKYKVETLQK RNSNDGKDLD RTNDPLKNRG TEIPQNSSNL 

      1450       1460       1470       1480       1490       1500 
ETDPFIRDSF DSDSGSSFRS DSDDLDSQLG ILPFTKMNKK LQEQTSQEFD DSIDPLYKIG 

      1510       1520       1530       1540       1550       1560 
SSTPGSSRLH GSFSKYIRPN SQREDGSEYV NISSLENMVS PNALPPVDYV MKSIYRTSVL 

      1570       1580       1590       1600       1610       1620 
VNDSNLMTRT KEAIELSKVL KKLKKKVLQD ISQMDDEMRE TGKPIFARGS SSPTLSRQHS 

      1630       1640       1650       1660       1670       1680 
DVATPLKQQE NTRPALKFAS SSPISEGFRK SSIKFSQAPS TQISPRTSVT DFTASQQRRQ 

      1690       1700       1710       1720       1730       1740 
HMNKRFSTQT THSTSALFMN PAFMPSAVNT GRKESEGHCE DRSATANRTN RKEDATTNDN 

      1750 
DNIAPFPFFG KRR 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Identification of a new class of negative regulators affecting sporulation-specific gene expression in yeast."
Benni M.L., Neigeborn L.
Genetics 147:1351-1366(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[4]"Candida albicans Mds3p, a conserved regulator of pH responses and virulence identified through insertional mutagenesis."
Davis D.A., Bruno V.M., Loza L., Filler S.G., Mitchell A.P.
Genetics 162:1573-1581(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1664, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1307 AND SER-1356, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1664, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 and spore morphogenesis in Saccharomyces cerevisiae."
McDonald C.M., Wagner M., Dunham M.J., Shin M.E., Ahmed N.T., Winter E.
Genetics 181:511-523(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-298; SER-838; SER-1289; SER-1307 AND SER-1664, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18916 Genomic DNA. Translation: AAC03230.1.
BK006939 Genomic DNA. Translation: DAA07792.1.
PIRS30855.
RefSeqNP_011058.3. NM_001179022.3.

3D structure databases

ProteinModelPortalP32634.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36876. 24 interactions.
DIPDIP-2381N.
IntActP32634. 12 interactions.
MINTMINT-690039.
STRING4932.YER132C.

Proteomic databases

MaxQBP32634.
PaxDbP32634.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER132C; YER132C; YER132C.
GeneID856869.
KEGGsce:YER132C.

Organism-specific databases

CYGDYER132c.
SGDS000000934. PMD1.

Phylogenomic databases

eggNOGNOG41848.
GeneTreeENSGT00530000066388.
HOGENOMHOG000113513.
OMAPLEMEST.
OrthoDBEOG7HQNHW.

Enzyme and pathway databases

BioCycYEAST:G3O-30295-MONOMER.

Gene expression databases

GenevestigatorP32634.

Family and domain databases

Gene3D2.120.10.80. 3 hits.
InterProIPR015915. Kelch-typ_b-propeller.
[Graphical view]
ProtoNetSearch...

Other

NextBio983237.

Entry information

Entry namePMD1_YEAST
AccessionPrimary (citable) accession number: P32634
Secondary accession number(s): D3DM38
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 14, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families