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P32634

- PMD1_YEAST

UniProt

P32634 - PMD1_YEAST

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Protein

Negative regulator of sporulation PMD1

Gene
PMD1, YER132C, SYGP-ORF50
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Negatively regulates early sporulation-specific genes. Seems to exert its function by positively regulating the Ras/cAMP pathway. Required for growth under alkaline conditions. Acts synergetically with MDS3.2 Publications

GO - Biological processi

  1. meiotic nuclear division Source: UniProtKB-KW
  2. sporulation resulting in formation of a cellular spore Source: SGD
Complete GO annotation...

Keywords - Biological processi

Meiosis, Sporulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30295-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Negative regulator of sporulation PMD1
Gene namesi
Name:PMD1
Ordered Locus Names:YER132C
ORF Names:SYGP-ORF50
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER132c.
SGDiS000000934. PMD1.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17531753Negative regulator of sporulation PMD1PRO_0000119141Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei298 – 2981Phosphothreonine1 Publication
Modified residuei838 – 8381Phosphoserine1 Publication
Modified residuei1289 – 12891Phosphoserine1 Publication
Modified residuei1307 – 13071Phosphoserine2 Publications
Modified residuei1356 – 13561Phosphoserine1 Publication
Modified residuei1664 – 16641Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32634.
PaxDbiP32634.

Expressioni

Gene expression databases

GenevestigatoriP32634.

Interactioni

Protein-protein interaction databases

BioGridi36876. 24 interactions.
DIPiDIP-2381N.
IntActiP32634. 12 interactions.
MINTiMINT-690039.
STRINGi4932.YER132C.

Structurei

3D structure databases

ProteinModelPortaliP32634.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati143 – 19856Kelch 1Add
BLAST
Repeati206 – 25348Kelch 2Add
BLAST

Sequence similaritiesi

Contains 2 Kelch repeats.

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiNOG41848.
GeneTreeiENSGT00530000066388.
HOGENOMiHOG000113513.
OMAiPLEMEST.
OrthoDBiEOG7HQNHW.

Family and domain databases

Gene3Di2.120.10.80. 3 hits.
InterProiIPR015915. Kelch-typ_b-propeller.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32634-1 [UniParc]FASTAAdd to Basket

« Hide

MTVLQPPSSV CYPLNLPIVP NPNLDEATRK KLTLECRTGA AVELARSGVF     50
VHGGLTLPLN LTIINSLQLQ KELILYFGKQ KDRNADFKTL ADWISPEIFF 100
LDLISRTWQR INTTIDTTSE NELNNGLSFK ERLFHSMCFT ESNIYIFGGL 150
MVSPHNGYEL IATNELWKLD LKTKCWSLIS ENPQITRRFN HSMHVLNENN 200
ENQDTKLIIV GGLDNMDIPV KKIDIFNLRT SLWESESKSD ENPASKGSSK 250
ILVNIDGMPI SLSHDSNFSV LIENNQAEIP TLALYYPQRE ANTSRRGTDD 300
GSFSTYAHDL DDKSKLPKHH HHHHGDLKYF ESDDADENAV KTLMSPIVIL 350
PLLGNSQGAR MTSNPTQNNK ENSILQVPFH LQYPSGNYFN YNIVVIGFYP 400
DPQPSNLHCF IYNIASGKWI RVNIACTECS ISMHRFWKLL IWKSHHQALL 450
LGTRTDDFCS PSVQKFDHIL SFSLPMLNGY NKLVNTKHTR TNNGIANSHN 500
LNVNLSLYDH LPYSNSSTIE HTNPYTVTQG YSLDDSGIPR LTSTATSQFE 550
NYSRYITVPL EMESTSSIFP PYAMVLGKDA LEIFGKTLSD FEFITADGDS 600
IGVPVYLLRK RWGRYFDSLL SNGYANTSFN YEFNGDTSNI ISFSPHTASK 650
TTKFGNSSQS SNGSLEKYFS KNGNSKSNSN TSLKKPHSVD FTSSTSSPKQ 700
RAISHNKLSP SEPILCADEE DSRSNTLKQH ATGDTGLKET GTSNKRPIST 750
TCSSTGMVFR VPFQDMKNSK LGLSEQSGRS TRASSVSPPP VYKKSTNDGN 800
DSNCTLSNTP LVYRRASTVG TTTNSSVDDG FSSIRRASHP LQSYIIAKSS 850
PSSISKASPA EKAFSRRKSS ALRFIASPNQ SRQTSFASTA STASVVSSTS 900
GRRRNSNQIS HLGSSASLPN SPILPVLNIP LPPQEKIPLE PLPPVPKAPS 950
RRSSSLAEYV QFGRDSPVAS RRSSHSTRKS SSSDARRISN SSLLRNTLDS 1000
QLLSNSYGSD IPYEASIQEY GMNNGRDEEE DGDNQDYGCI SPSNIRPIFS 1050
TINAININGN FKEGEFFSSK SYINNEKSRR LSYISNPESV ESTNSNNNAI 1100
IELEPLLTPR SLYMPWSTAS VRAFAEFFYT AQINGKWLLA PVTLDLLIMA 1150
KIYEIPILYE LITEVLYKII SKKEEGLSVT CEALLNLFQQ KVSRYCNENE 1200
GKIRKQLDSS ESYQDTLEIK RSLANIDNGY VDSYLLRNTS MAQSIHYTDD 1250
SNGETEIDMH HTGISSIGSL ANRAVPTVFA GGPRDSHNSI GSIAFPSNSG 1300
VQNIRRSVSL FSPATKKKSS LSRETDPLDT SDQFTDDVPD SGPVSRQQNF 1350
PRRSSSFTET VPTEPTRYNY QNLDSSKSNR ASDDKEEQNE QATLQDISNF 1400
DKYKVETLQK RNSNDGKDLD RTNDPLKNRG TEIPQNSSNL ETDPFIRDSF 1450
DSDSGSSFRS DSDDLDSQLG ILPFTKMNKK LQEQTSQEFD DSIDPLYKIG 1500
SSTPGSSRLH GSFSKYIRPN SQREDGSEYV NISSLENMVS PNALPPVDYV 1550
MKSIYRTSVL VNDSNLMTRT KEAIELSKVL KKLKKKVLQD ISQMDDEMRE 1600
TGKPIFARGS SSPTLSRQHS DVATPLKQQE NTRPALKFAS SSPISEGFRK 1650
SSIKFSQAPS TQISPRTSVT DFTASQQRRQ HMNKRFSTQT THSTSALFMN 1700
PAFMPSAVNT GRKESEGHCE DRSATANRTN RKEDATTNDN DNIAPFPFFG 1750
KRR 1753
Length:1,753
Mass (Da):195,383
Last modified:October 1, 1993 - v1
Checksum:iE4252998C6C2508B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18916 Genomic DNA. Translation: AAC03230.1.
BK006939 Genomic DNA. Translation: DAA07792.1.
PIRiS30855.
RefSeqiNP_011058.3. NM_001179022.3.

Genome annotation databases

EnsemblFungiiYER132C; YER132C; YER132C.
GeneIDi856869.
KEGGisce:YER132C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18916 Genomic DNA. Translation: AAC03230.1 .
BK006939 Genomic DNA. Translation: DAA07792.1 .
PIRi S30855.
RefSeqi NP_011058.3. NM_001179022.3.

3D structure databases

ProteinModelPortali P32634.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36876. 24 interactions.
DIPi DIP-2381N.
IntActi P32634. 12 interactions.
MINTi MINT-690039.
STRINGi 4932.YER132C.

Proteomic databases

MaxQBi P32634.
PaxDbi P32634.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER132C ; YER132C ; YER132C .
GeneIDi 856869.
KEGGi sce:YER132C.

Organism-specific databases

CYGDi YER132c.
SGDi S000000934. PMD1.

Phylogenomic databases

eggNOGi NOG41848.
GeneTreei ENSGT00530000066388.
HOGENOMi HOG000113513.
OMAi PLEMEST.
OrthoDBi EOG7HQNHW.

Enzyme and pathway databases

BioCyci YEAST:G3O-30295-MONOMER.

Miscellaneous databases

NextBioi 983237.

Gene expression databases

Genevestigatori P32634.

Family and domain databases

Gene3Di 2.120.10.80. 3 hits.
InterProi IPR015915. Kelch-typ_b-propeller.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Identification of a new class of negative regulators affecting sporulation-specific gene expression in yeast."
    Benni M.L., Neigeborn L.
    Genetics 147:1351-1366(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "Candida albicans Mds3p, a conserved regulator of pH responses and virulence identified through insertional mutagenesis."
    Davis D.A., Bruno V.M., Loza L., Filler S.G., Mitchell A.P.
    Genetics 162:1573-1581(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1664, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1307 AND SER-1356, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1664, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 and spore morphogenesis in Saccharomyces cerevisiae."
    McDonald C.M., Wagner M., Dunham M.J., Shin M.E., Ahmed N.T., Winter E.
    Genetics 181:511-523(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-298; SER-838; SER-1289; SER-1307 AND SER-1664, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPMD1_YEAST
AccessioniPrimary (citable) accession number: P32634
Secondary accession number(s): D3DM38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 14, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 815 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

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