Negative regulator of sporulation PMD1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Sequence or UniProt identifier

>sp|P32634|PMD1_YEAST Negative regulator of sporulation PMD1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PMD1 PE=1 SV=1 MTVLQPPSSVCYPLNLPIVPNPNLDEATRKKLTLECRTGAAVELARSGVFVHGGLTLPLN LTIINSLQLQKELILYFGKQKDRNADFKTLADWISPEIFFLDLISRTWQRINTTIDTTSE NELNNGLSFKERLFHSMCFTESNIYIFGGLMVSPHNGYELIATNELWKLDLKTKCWSLIS ENPQITRRFNHSMHVLNENNENQDTKLIIVGGLDNMDIPVKKIDIFNLRTSLWESESKSD ENPASKGSSKILVNIDGMPISLSHDSNFSVLIENNQAEIPTLALYYPQREANTSRRGTDD GSFSTYAHDLDDKSKLPKHHHHHHGDLKYFESDDADENAVKTLMSPIVILPLLGNSQGAR MTSNPTQNNKENSILQVPFHLQYPSGNYFNYNIVVIGFYPDPQPSNLHCFIYNIASGKWI RVNIACTECSISMHRFWKLLIWKSHHQALLLGTRTDDFCSPSVQKFDHILSFSLPMLNGY NKLVNTKHTRTNNGIANSHNLNVNLSLYDHLPYSNSSTIEHTNPYTVTQGYSLDDSGIPR LTSTATSQFENYSRYITVPLEMESTSSIFPPYAMVLGKDALEIFGKTLSDFEFITADGDS IGVPVYLLRKRWGRYFDSLLSNGYANTSFNYEFNGDTSNIISFSPHTASKTTKFGNSSQS SNGSLEKYFSKNGNSKSNSNTSLKKPHSVDFTSSTSSPKQRAISHNKLSPSEPILCADEE DSRSNTLKQHATGDTGLKETGTSNKRPISTTCSSTGMVFRVPFQDMKNSKLGLSEQSGRS TRASSVSPPPVYKKSTNDGNDSNCTLSNTPLVYRRASTVGTTTNSSVDDGFSSIRRASHP LQSYIIAKSSPSSISKASPAEKAFSRRKSSALRFIASPNQSRQTSFASTASTASVVSSTS GRRRNSNQISHLGSSASLPNSPILPVLNIPLPPQEKIPLEPLPPVPKAPSRRSSSLAEYV QFGRDSPVASRRSSHSTRKSSSSDARRISNSSLLRNTLDSQLLSNSYGSDIPYEASIQEY GMNNGRDEEEDGDNQDYGCISPSNIRPIFSTINAININGNFKEGEFFSSKSYINNEKSRR LSYISNPESVESTNSNNNAIIELEPLLTPRSLYMPWSTASVRAFAEFFYTAQINGKWLLA PVTLDLLIMAKIYEIPILYELITEVLYKIISKKEEGLSVTCEALLNLFQQKVSRYCNENE GKIRKQLDSSESYQDTLEIKRSLANIDNGYVDSYLLRNTSMAQSIHYTDDSNGETEIDMH HTGISSIGSLANRAVPTVFAGGPRDSHNSIGSIAFPSNSGVQNIRRSVSLFSPATKKKSS LSRETDPLDTSDQFTDDVPDSGPVSRQQNFPRRSSSFTETVPTEPTRYNYQNLDSSKSNR ASDDKEEQNEQATLQDISNFDKYKVETLQKRNSNDGKDLDRTNDPLKNRGTEIPQNSSNL ETDPFIRDSFDSDSGSSFRSDSDDLDSQLGILPFTKMNKKLQEQTSQEFDDSIDPLYKIG SSTPGSSRLHGSFSKYIRPNSQREDGSEYVNISSLENMVSPNALPPVDYVMKSIYRTSVL VNDSNLMTRTKEAIELSKVLKKLKKKVLQDISQMDDEMRETGKPIFARGSSSPTLSRQHS DVATPLKQQENTRPALKFASSSPISEGFRKSSIKFSQAPSTQISPRTSVTDFTASQQRRQ HMNKRFSTQTTHSTSALFMNPAFMPSAVNTGRKESEGHCEDRSATANRTNRKEDATTNDN DNIAPFPFFGKRR

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P32634 (PMD1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Negative regulator of sporulation PMD1

Gene names

Name:	PMD1
Ordered Locus Names:	YER132C
ORF Names:	SYGP-ORF50

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1753 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Negatively regulates early sporulation-specific genes. Seems to exert its function by positively regulating the Ras/cAMP pathway. Required for growth under alkaline conditions. Acts synergetically with MDS3. Ref.4 Ref.11
Subcellular location	Cytoplasm Ref.5.
Miscellaneous	Present with 815 molecules/cell in log phase SD medium.
Sequence similarities	Contains 2 Kelch repeats.

Ontologies

Keywords
Biological process	Meiosis Sporulation
Cellular component	Cytoplasm
Domain	Kelch repeat Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	meiosis Inferred from electronic annotation. Source: UniProtKB-KW sporulation resulting in formation of a cellular spore Inferred from genetic interaction Ref.3. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1753

1753

Negative regulator of sporulation PMD1

PRO_0000119141

Regions

Repeat

143 – 198

Kelch 1

Repeat

206 – 253

Kelch 2

Amino acid modifications

Modified residue

546

Phosphothreonine Ref.10

Modified residue

697

Phosphoserine Ref.10

Modified residue

711

Phosphoserine Ref.10

Modified residue

784

Phosphoserine Ref.9

Modified residue

785

Phosphoserine Ref.9

Modified residue

787

Phosphoserine Ref.9

Modified residue

877

Phosphoserine Ref.7

Modified residue

989

Phosphoserine Ref.9

Modified residue

992

Phosphoserine Ref.9 Ref.10

Modified residue

1286

Phosphoserine Ref.10

Modified residue

1292

Phosphoserine Ref.7

Modified residue

1307

Phosphoserine Ref.8 Ref.10

Modified residue

1309

Phosphoserine Ref.10

Modified residue

1355

Phosphoserine Ref.10

Modified residue

1356

Phosphoserine Ref.8 Ref.9 Ref.10

Modified residue

1486

Phosphoserine Ref.10

Modified residue

1611

Phosphoserine Ref.10

Modified residue

1612

Phosphoserine Ref.10

Modified residue

1642

Phosphoserine Ref.7 Ref.10

Modified residue

1664

Phosphoserine Ref.7 Ref.10

Modified residue

1668

Phosphoserine Ref.10

Modified residue

1670

Phosphothreonine Ref.9

Modified residue

1675

Phosphoserine Ref.9

Sequences

Sequence

Length

Mass (Da)

Tools

P32634 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: E4252998C6C2508B
Show »

FASTA

1,753

195,383

        10         20         30         40         50         60 
MTVLQPPSSV CYPLNLPIVP NPNLDEATRK KLTLECRTGA AVELARSGVF VHGGLTLPLN 

        70         80         90        100        110        120 
LTIINSLQLQ KELILYFGKQ KDRNADFKTL ADWISPEIFF LDLISRTWQR INTTIDTTSE 

       130        140        150        160        170        180 
NELNNGLSFK ERLFHSMCFT ESNIYIFGGL MVSPHNGYEL IATNELWKLD LKTKCWSLIS 

       190        200        210        220        230        240 
ENPQITRRFN HSMHVLNENN ENQDTKLIIV GGLDNMDIPV KKIDIFNLRT SLWESESKSD 

       250        260        270        280        290        300 
ENPASKGSSK ILVNIDGMPI SLSHDSNFSV LIENNQAEIP TLALYYPQRE ANTSRRGTDD 

       310        320        330        340        350        360 
GSFSTYAHDL DDKSKLPKHH HHHHGDLKYF ESDDADENAV KTLMSPIVIL PLLGNSQGAR 

       370        380        390        400        410        420 
MTSNPTQNNK ENSILQVPFH LQYPSGNYFN YNIVVIGFYP DPQPSNLHCF IYNIASGKWI 

       430        440        450        460        470        480 
RVNIACTECS ISMHRFWKLL IWKSHHQALL LGTRTDDFCS PSVQKFDHIL SFSLPMLNGY 

       490        500        510        520        530        540 
NKLVNTKHTR TNNGIANSHN LNVNLSLYDH LPYSNSSTIE HTNPYTVTQG YSLDDSGIPR 

       550        560        570        580        590        600 
LTSTATSQFE NYSRYITVPL EMESTSSIFP PYAMVLGKDA LEIFGKTLSD FEFITADGDS 

       610        620        630        640        650        660 
IGVPVYLLRK RWGRYFDSLL SNGYANTSFN YEFNGDTSNI ISFSPHTASK TTKFGNSSQS 

       670        680        690        700        710        720 
SNGSLEKYFS KNGNSKSNSN TSLKKPHSVD FTSSTSSPKQ RAISHNKLSP SEPILCADEE 

       730        740        750        760        770        780 
DSRSNTLKQH ATGDTGLKET GTSNKRPIST TCSSTGMVFR VPFQDMKNSK LGLSEQSGRS 

       790        800        810        820        830        840 
TRASSVSPPP VYKKSTNDGN DSNCTLSNTP LVYRRASTVG TTTNSSVDDG FSSIRRASHP 

       850        860        870        880        890        900 
LQSYIIAKSS PSSISKASPA EKAFSRRKSS ALRFIASPNQ SRQTSFASTA STASVVSSTS 

       910        920        930        940        950        960 
GRRRNSNQIS HLGSSASLPN SPILPVLNIP LPPQEKIPLE PLPPVPKAPS RRSSSLAEYV 

       970        980        990       1000       1010       1020 
QFGRDSPVAS RRSSHSTRKS SSSDARRISN SSLLRNTLDS QLLSNSYGSD IPYEASIQEY 

      1030       1040       1050       1060       1070       1080 
GMNNGRDEEE DGDNQDYGCI SPSNIRPIFS TINAININGN FKEGEFFSSK SYINNEKSRR 

      1090       1100       1110       1120       1130       1140 
LSYISNPESV ESTNSNNNAI IELEPLLTPR SLYMPWSTAS VRAFAEFFYT AQINGKWLLA 

      1150       1160       1170       1180       1190       1200 
PVTLDLLIMA KIYEIPILYE LITEVLYKII SKKEEGLSVT CEALLNLFQQ KVSRYCNENE 

      1210       1220       1230       1240       1250       1260 
GKIRKQLDSS ESYQDTLEIK RSLANIDNGY VDSYLLRNTS MAQSIHYTDD SNGETEIDMH 

      1270       1280       1290       1300       1310       1320 
HTGISSIGSL ANRAVPTVFA GGPRDSHNSI GSIAFPSNSG VQNIRRSVSL FSPATKKKSS 

      1330       1340       1350       1360       1370       1380 
LSRETDPLDT SDQFTDDVPD SGPVSRQQNF PRRSSSFTET VPTEPTRYNY QNLDSSKSNR 

      1390       1400       1410       1420       1430       1440 
ASDDKEEQNE QATLQDISNF DKYKVETLQK RNSNDGKDLD RTNDPLKNRG TEIPQNSSNL 

      1450       1460       1470       1480       1490       1500 
ETDPFIRDSF DSDSGSSFRS DSDDLDSQLG ILPFTKMNKK LQEQTSQEFD DSIDPLYKIG 

      1510       1520       1530       1540       1550       1560 
SSTPGSSRLH GSFSKYIRPN SQREDGSEYV NISSLENMVS PNALPPVDYV MKSIYRTSVL 

      1570       1580       1590       1600       1610       1620 
VNDSNLMTRT KEAIELSKVL KKLKKKVLQD ISQMDDEMRE TGKPIFARGS SSPTLSRQHS 

      1630       1640       1650       1660       1670       1680 
DVATPLKQQE NTRPALKFAS SSPISEGFRK SSIKFSQAPS TQISPRTSVT DFTASQQRRQ 

      1690       1700       1710       1720       1730       1740 
HMNKRFSTQT THSTSALFMN PAFMPSAVNT GRKESEGHCE DRSATANRTN RKEDATTNDN 

      1750 
DNIAPFPFFG KRR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Identification of a new class of negative regulators affecting sporulation-specific gene expression in yeast." Benni M.L., Neigeborn L. Genetics 147:1351-1366(1997) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Candida albicans Mds3p, a conserved regulator of pH responses and virulence identified through insertional mutagenesis." Davis D.A., Bruno V.M., Loza L., Filler S.G., Mitchell A.P. Genetics 162:1573-1581(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-877; SER-1292; SER-1642 AND SER-1664, MASS SPECTROMETRY. Strain: YAL6B.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1307 AND SER-1356, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-784; SER-785; SER-787; SER-989; SER-992; SER-1356; THR-1670 AND SER-1675, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-546; SER-697; SER-711; SER-992; SER-1286; SER-1307; SER-1309; SER-1355; SER-1356; SER-1486; SER-1611; SER-1612; SER-1642; SER-1664 AND SER-1668, MASS SPECTROMETRY.
[11]	"The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 and spore morphogenesis in Saccharomyces cerevisiae." McDonald C.M., Wagner M., Dunham M.J., Shin M.E., Ahmed N.T., Winter E. Genetics 181:511-523(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18916 Genomic DNA. Translation: AAC03230.1. BK006939 Genomic DNA. Translation: DAA07792.1.
PIR	S30855.
RefSeq	NP_011058.3. NM_001179022.3.
3D structure databases
ProteinModelPortal	P32634.
SMR	P32634. Positions 129-233, 377-467.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2381N.
IntAct	P32634. 10 interactions.
MINT	MINT-690039.
STRING	4932.YER132C.
Proteomic databases
PaxDb	P32634.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER132C; YER132C; YER132C.
GeneID	856869.
KEGG	sce:YER132C. sce:YER137C.
Organism-specific databases
CYGD	YER132c.
SGD	S000000934. PMD1.
Phylogenomic databases
eggNOG	NOG41848.
GeneTree	ENSGT00530000066388.
HOGENOM	HOG000113513.
OMA	PLEMEST.
OrthoDB	EOG437VP0.
Gene expression databases
Genevestigator	P32634.
GermOnline	YER132C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.120.10.80. 3 hits.
InterPro	IPR015915. Kelch-typ_b-propeller. [Graphical view]
ProtoNet	Search...
Other
NextBio	983237.

Entry information

Entry name

PMD1_YEAST

Accession

Primary (citable) accession number: P32634
Secondary accession number(s): D3DM38

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents