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P32634

- PMD1_YEAST

UniProt

P32634 - PMD1_YEAST

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Protein

Negative regulator of sporulation PMD1

Gene

PMD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Negatively regulates early sporulation-specific genes. Seems to exert its function by positively regulating the Ras/cAMP pathway. Required for growth under alkaline conditions. Acts synergetically with MDS3.2 Publications

GO - Biological processi

  1. meiotic nuclear division Source: UniProtKB-KW
  2. sporulation resulting in formation of a cellular spore Source: SGD
Complete GO annotation...

Keywords - Biological processi

Meiosis, Sporulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30295-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Negative regulator of sporulation PMD1
Gene namesi
Name:PMD1
Ordered Locus Names:YER132C
ORF Names:SYGP-ORF50
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYER132c.
SGDiS000000934. PMD1.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17531753Negative regulator of sporulation PMD1PRO_0000119141Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei298 – 2981Phosphothreonine1 Publication
Modified residuei838 – 8381Phosphoserine1 Publication
Modified residuei1289 – 12891Phosphoserine1 Publication
Modified residuei1307 – 13071Phosphoserine2 Publications
Modified residuei1356 – 13561Phosphoserine1 Publication
Modified residuei1664 – 16641Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32634.
PaxDbiP32634.

Expressioni

Gene expression databases

GenevestigatoriP32634.

Interactioni

Protein-protein interaction databases

BioGridi36876. 24 interactions.
DIPiDIP-2381N.
IntActiP32634. 12 interactions.
MINTiMINT-690039.
STRINGi4932.YER132C.

Structurei

3D structure databases

ProteinModelPortaliP32634.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati143 – 19856Kelch 1Add
BLAST
Repeati206 – 25348Kelch 2Add
BLAST

Sequence similaritiesi

Contains 2 Kelch repeats.Curated

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiNOG41848.
GeneTreeiENSGT00530000066388.
HOGENOMiHOG000113513.
InParanoidiP32634.
OMAiPLEMEST.
OrthoDBiEOG7HQNHW.

Family and domain databases

Gene3Di2.120.10.80. 3 hits.
InterProiIPR015915. Kelch-typ_b-propeller.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32634-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTVLQPPSSV CYPLNLPIVP NPNLDEATRK KLTLECRTGA AVELARSGVF
60 70 80 90 100
VHGGLTLPLN LTIINSLQLQ KELILYFGKQ KDRNADFKTL ADWISPEIFF
110 120 130 140 150
LDLISRTWQR INTTIDTTSE NELNNGLSFK ERLFHSMCFT ESNIYIFGGL
160 170 180 190 200
MVSPHNGYEL IATNELWKLD LKTKCWSLIS ENPQITRRFN HSMHVLNENN
210 220 230 240 250
ENQDTKLIIV GGLDNMDIPV KKIDIFNLRT SLWESESKSD ENPASKGSSK
260 270 280 290 300
ILVNIDGMPI SLSHDSNFSV LIENNQAEIP TLALYYPQRE ANTSRRGTDD
310 320 330 340 350
GSFSTYAHDL DDKSKLPKHH HHHHGDLKYF ESDDADENAV KTLMSPIVIL
360 370 380 390 400
PLLGNSQGAR MTSNPTQNNK ENSILQVPFH LQYPSGNYFN YNIVVIGFYP
410 420 430 440 450
DPQPSNLHCF IYNIASGKWI RVNIACTECS ISMHRFWKLL IWKSHHQALL
460 470 480 490 500
LGTRTDDFCS PSVQKFDHIL SFSLPMLNGY NKLVNTKHTR TNNGIANSHN
510 520 530 540 550
LNVNLSLYDH LPYSNSSTIE HTNPYTVTQG YSLDDSGIPR LTSTATSQFE
560 570 580 590 600
NYSRYITVPL EMESTSSIFP PYAMVLGKDA LEIFGKTLSD FEFITADGDS
610 620 630 640 650
IGVPVYLLRK RWGRYFDSLL SNGYANTSFN YEFNGDTSNI ISFSPHTASK
660 670 680 690 700
TTKFGNSSQS SNGSLEKYFS KNGNSKSNSN TSLKKPHSVD FTSSTSSPKQ
710 720 730 740 750
RAISHNKLSP SEPILCADEE DSRSNTLKQH ATGDTGLKET GTSNKRPIST
760 770 780 790 800
TCSSTGMVFR VPFQDMKNSK LGLSEQSGRS TRASSVSPPP VYKKSTNDGN
810 820 830 840 850
DSNCTLSNTP LVYRRASTVG TTTNSSVDDG FSSIRRASHP LQSYIIAKSS
860 870 880 890 900
PSSISKASPA EKAFSRRKSS ALRFIASPNQ SRQTSFASTA STASVVSSTS
910 920 930 940 950
GRRRNSNQIS HLGSSASLPN SPILPVLNIP LPPQEKIPLE PLPPVPKAPS
960 970 980 990 1000
RRSSSLAEYV QFGRDSPVAS RRSSHSTRKS SSSDARRISN SSLLRNTLDS
1010 1020 1030 1040 1050
QLLSNSYGSD IPYEASIQEY GMNNGRDEEE DGDNQDYGCI SPSNIRPIFS
1060 1070 1080 1090 1100
TINAININGN FKEGEFFSSK SYINNEKSRR LSYISNPESV ESTNSNNNAI
1110 1120 1130 1140 1150
IELEPLLTPR SLYMPWSTAS VRAFAEFFYT AQINGKWLLA PVTLDLLIMA
1160 1170 1180 1190 1200
KIYEIPILYE LITEVLYKII SKKEEGLSVT CEALLNLFQQ KVSRYCNENE
1210 1220 1230 1240 1250
GKIRKQLDSS ESYQDTLEIK RSLANIDNGY VDSYLLRNTS MAQSIHYTDD
1260 1270 1280 1290 1300
SNGETEIDMH HTGISSIGSL ANRAVPTVFA GGPRDSHNSI GSIAFPSNSG
1310 1320 1330 1340 1350
VQNIRRSVSL FSPATKKKSS LSRETDPLDT SDQFTDDVPD SGPVSRQQNF
1360 1370 1380 1390 1400
PRRSSSFTET VPTEPTRYNY QNLDSSKSNR ASDDKEEQNE QATLQDISNF
1410 1420 1430 1440 1450
DKYKVETLQK RNSNDGKDLD RTNDPLKNRG TEIPQNSSNL ETDPFIRDSF
1460 1470 1480 1490 1500
DSDSGSSFRS DSDDLDSQLG ILPFTKMNKK LQEQTSQEFD DSIDPLYKIG
1510 1520 1530 1540 1550
SSTPGSSRLH GSFSKYIRPN SQREDGSEYV NISSLENMVS PNALPPVDYV
1560 1570 1580 1590 1600
MKSIYRTSVL VNDSNLMTRT KEAIELSKVL KKLKKKVLQD ISQMDDEMRE
1610 1620 1630 1640 1650
TGKPIFARGS SSPTLSRQHS DVATPLKQQE NTRPALKFAS SSPISEGFRK
1660 1670 1680 1690 1700
SSIKFSQAPS TQISPRTSVT DFTASQQRRQ HMNKRFSTQT THSTSALFMN
1710 1720 1730 1740 1750
PAFMPSAVNT GRKESEGHCE DRSATANRTN RKEDATTNDN DNIAPFPFFG

KRR
Length:1,753
Mass (Da):195,383
Last modified:October 1, 1993 - v1
Checksum:iE4252998C6C2508B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18916 Genomic DNA. Translation: AAC03230.1.
BK006939 Genomic DNA. Translation: DAA07792.1.
PIRiS30855.
RefSeqiNP_011058.3. NM_001179022.3.

Genome annotation databases

EnsemblFungiiYER132C; YER132C; YER132C.
GeneIDi856869.
KEGGisce:YER132C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18916 Genomic DNA. Translation: AAC03230.1 .
BK006939 Genomic DNA. Translation: DAA07792.1 .
PIRi S30855.
RefSeqi NP_011058.3. NM_001179022.3.

3D structure databases

ProteinModelPortali P32634.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36876. 24 interactions.
DIPi DIP-2381N.
IntActi P32634. 12 interactions.
MINTi MINT-690039.
STRINGi 4932.YER132C.

Proteomic databases

MaxQBi P32634.
PaxDbi P32634.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER132C ; YER132C ; YER132C .
GeneIDi 856869.
KEGGi sce:YER132C.

Organism-specific databases

CYGDi YER132c.
SGDi S000000934. PMD1.

Phylogenomic databases

eggNOGi NOG41848.
GeneTreei ENSGT00530000066388.
HOGENOMi HOG000113513.
InParanoidi P32634.
OMAi PLEMEST.
OrthoDBi EOG7HQNHW.

Enzyme and pathway databases

BioCyci YEAST:G3O-30295-MONOMER.

Miscellaneous databases

NextBioi 983237.

Gene expression databases

Genevestigatori P32634.

Family and domain databases

Gene3Di 2.120.10.80. 3 hits.
InterProi IPR015915. Kelch-typ_b-propeller.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Identification of a new class of negative regulators affecting sporulation-specific gene expression in yeast."
    Benni M.L., Neigeborn L.
    Genetics 147:1351-1366(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "Candida albicans Mds3p, a conserved regulator of pH responses and virulence identified through insertional mutagenesis."
    Davis D.A., Bruno V.M., Loza L., Filler S.G., Mitchell A.P.
    Genetics 162:1573-1581(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1664, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1307 AND SER-1356, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1664, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 and spore morphogenesis in Saccharomyces cerevisiae."
    McDonald C.M., Wagner M., Dunham M.J., Shin M.E., Ahmed N.T., Winter E.
    Genetics 181:511-523(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-298; SER-838; SER-1289; SER-1307 AND SER-1664, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPMD1_YEAST
AccessioniPrimary (citable) accession number: P32634
Secondary accession number(s): D3DM38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 29, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 815 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3