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P32634

- PMD1_YEAST

UniProt

P32634 - PMD1_YEAST

Protein

Negative regulator of sporulation PMD1

Gene

PMD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Negatively regulates early sporulation-specific genes. Seems to exert its function by positively regulating the Ras/cAMP pathway. Required for growth under alkaline conditions. Acts synergetically with MDS3.2 Publications

    GO - Biological processi

    1. meiotic nuclear division Source: UniProtKB-KW
    2. sporulation resulting in formation of a cellular spore Source: SGD

    Keywords - Biological processi

    Meiosis, Sporulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30295-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Negative regulator of sporulation PMD1
    Gene namesi
    Name:PMD1
    Ordered Locus Names:YER132C
    ORF Names:SYGP-ORF50
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYER132c.
    SGDiS000000934. PMD1.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 17531753Negative regulator of sporulation PMD1PRO_0000119141Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei298 – 2981Phosphothreonine1 Publication
    Modified residuei838 – 8381Phosphoserine1 Publication
    Modified residuei1289 – 12891Phosphoserine1 Publication
    Modified residuei1307 – 13071Phosphoserine2 Publications
    Modified residuei1356 – 13561Phosphoserine1 Publication
    Modified residuei1664 – 16641Phosphoserine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP32634.
    PaxDbiP32634.

    Expressioni

    Gene expression databases

    GenevestigatoriP32634.

    Interactioni

    Protein-protein interaction databases

    BioGridi36876. 24 interactions.
    DIPiDIP-2381N.
    IntActiP32634. 12 interactions.
    MINTiMINT-690039.
    STRINGi4932.YER132C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32634.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati143 – 19856Kelch 1Add
    BLAST
    Repeati206 – 25348Kelch 2Add
    BLAST

    Sequence similaritiesi

    Contains 2 Kelch repeats.Curated

    Keywords - Domaini

    Kelch repeat, Repeat

    Phylogenomic databases

    eggNOGiNOG41848.
    GeneTreeiENSGT00530000066388.
    HOGENOMiHOG000113513.
    OMAiPLEMEST.
    OrthoDBiEOG7HQNHW.

    Family and domain databases

    Gene3Di2.120.10.80. 3 hits.
    InterProiIPR015915. Kelch-typ_b-propeller.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32634-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVLQPPSSV CYPLNLPIVP NPNLDEATRK KLTLECRTGA AVELARSGVF     50
    VHGGLTLPLN LTIINSLQLQ KELILYFGKQ KDRNADFKTL ADWISPEIFF 100
    LDLISRTWQR INTTIDTTSE NELNNGLSFK ERLFHSMCFT ESNIYIFGGL 150
    MVSPHNGYEL IATNELWKLD LKTKCWSLIS ENPQITRRFN HSMHVLNENN 200
    ENQDTKLIIV GGLDNMDIPV KKIDIFNLRT SLWESESKSD ENPASKGSSK 250
    ILVNIDGMPI SLSHDSNFSV LIENNQAEIP TLALYYPQRE ANTSRRGTDD 300
    GSFSTYAHDL DDKSKLPKHH HHHHGDLKYF ESDDADENAV KTLMSPIVIL 350
    PLLGNSQGAR MTSNPTQNNK ENSILQVPFH LQYPSGNYFN YNIVVIGFYP 400
    DPQPSNLHCF IYNIASGKWI RVNIACTECS ISMHRFWKLL IWKSHHQALL 450
    LGTRTDDFCS PSVQKFDHIL SFSLPMLNGY NKLVNTKHTR TNNGIANSHN 500
    LNVNLSLYDH LPYSNSSTIE HTNPYTVTQG YSLDDSGIPR LTSTATSQFE 550
    NYSRYITVPL EMESTSSIFP PYAMVLGKDA LEIFGKTLSD FEFITADGDS 600
    IGVPVYLLRK RWGRYFDSLL SNGYANTSFN YEFNGDTSNI ISFSPHTASK 650
    TTKFGNSSQS SNGSLEKYFS KNGNSKSNSN TSLKKPHSVD FTSSTSSPKQ 700
    RAISHNKLSP SEPILCADEE DSRSNTLKQH ATGDTGLKET GTSNKRPIST 750
    TCSSTGMVFR VPFQDMKNSK LGLSEQSGRS TRASSVSPPP VYKKSTNDGN 800
    DSNCTLSNTP LVYRRASTVG TTTNSSVDDG FSSIRRASHP LQSYIIAKSS 850
    PSSISKASPA EKAFSRRKSS ALRFIASPNQ SRQTSFASTA STASVVSSTS 900
    GRRRNSNQIS HLGSSASLPN SPILPVLNIP LPPQEKIPLE PLPPVPKAPS 950
    RRSSSLAEYV QFGRDSPVAS RRSSHSTRKS SSSDARRISN SSLLRNTLDS 1000
    QLLSNSYGSD IPYEASIQEY GMNNGRDEEE DGDNQDYGCI SPSNIRPIFS 1050
    TINAININGN FKEGEFFSSK SYINNEKSRR LSYISNPESV ESTNSNNNAI 1100
    IELEPLLTPR SLYMPWSTAS VRAFAEFFYT AQINGKWLLA PVTLDLLIMA 1150
    KIYEIPILYE LITEVLYKII SKKEEGLSVT CEALLNLFQQ KVSRYCNENE 1200
    GKIRKQLDSS ESYQDTLEIK RSLANIDNGY VDSYLLRNTS MAQSIHYTDD 1250
    SNGETEIDMH HTGISSIGSL ANRAVPTVFA GGPRDSHNSI GSIAFPSNSG 1300
    VQNIRRSVSL FSPATKKKSS LSRETDPLDT SDQFTDDVPD SGPVSRQQNF 1350
    PRRSSSFTET VPTEPTRYNY QNLDSSKSNR ASDDKEEQNE QATLQDISNF 1400
    DKYKVETLQK RNSNDGKDLD RTNDPLKNRG TEIPQNSSNL ETDPFIRDSF 1450
    DSDSGSSFRS DSDDLDSQLG ILPFTKMNKK LQEQTSQEFD DSIDPLYKIG 1500
    SSTPGSSRLH GSFSKYIRPN SQREDGSEYV NISSLENMVS PNALPPVDYV 1550
    MKSIYRTSVL VNDSNLMTRT KEAIELSKVL KKLKKKVLQD ISQMDDEMRE 1600
    TGKPIFARGS SSPTLSRQHS DVATPLKQQE NTRPALKFAS SSPISEGFRK 1650
    SSIKFSQAPS TQISPRTSVT DFTASQQRRQ HMNKRFSTQT THSTSALFMN 1700
    PAFMPSAVNT GRKESEGHCE DRSATANRTN RKEDATTNDN DNIAPFPFFG 1750
    KRR 1753
    Length:1,753
    Mass (Da):195,383
    Last modified:October 1, 1993 - v1
    Checksum:iE4252998C6C2508B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18916 Genomic DNA. Translation: AAC03230.1.
    BK006939 Genomic DNA. Translation: DAA07792.1.
    PIRiS30855.
    RefSeqiNP_011058.3. NM_001179022.3.

    Genome annotation databases

    EnsemblFungiiYER132C; YER132C; YER132C.
    GeneIDi856869.
    KEGGisce:YER132C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18916 Genomic DNA. Translation: AAC03230.1 .
    BK006939 Genomic DNA. Translation: DAA07792.1 .
    PIRi S30855.
    RefSeqi NP_011058.3. NM_001179022.3.

    3D structure databases

    ProteinModelPortali P32634.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36876. 24 interactions.
    DIPi DIP-2381N.
    IntActi P32634. 12 interactions.
    MINTi MINT-690039.
    STRINGi 4932.YER132C.

    Proteomic databases

    MaxQBi P32634.
    PaxDbi P32634.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER132C ; YER132C ; YER132C .
    GeneIDi 856869.
    KEGGi sce:YER132C.

    Organism-specific databases

    CYGDi YER132c.
    SGDi S000000934. PMD1.

    Phylogenomic databases

    eggNOGi NOG41848.
    GeneTreei ENSGT00530000066388.
    HOGENOMi HOG000113513.
    OMAi PLEMEST.
    OrthoDBi EOG7HQNHW.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30295-MONOMER.

    Miscellaneous databases

    NextBioi 983237.

    Gene expression databases

    Genevestigatori P32634.

    Family and domain databases

    Gene3Di 2.120.10.80. 3 hits.
    InterProi IPR015915. Kelch-typ_b-propeller.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Identification of a new class of negative regulators affecting sporulation-specific gene expression in yeast."
      Benni M.L., Neigeborn L.
      Genetics 147:1351-1366(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    4. "Candida albicans Mds3p, a conserved regulator of pH responses and virulence identified through insertional mutagenesis."
      Davis D.A., Bruno V.M., Loza L., Filler S.G., Mitchell A.P.
      Genetics 162:1573-1581(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1664, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1307 AND SER-1356, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1664, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 and spore morphogenesis in Saccharomyces cerevisiae."
      McDonald C.M., Wagner M., Dunham M.J., Shin M.E., Ahmed N.T., Winter E.
      Genetics 181:511-523(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-298; SER-838; SER-1289; SER-1307 AND SER-1664, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPMD1_YEAST
    AccessioniPrimary (citable) accession number: P32634
    Secondary accession number(s): D3DM38
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 815 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3