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Protein

Mannan polymerase II complex ANP1 subunit

Gene

ANP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the organization of the secretory pathway. Required to maintain a functional Golgi apparatus.
The M-Pol II complex possesses alpha-1,6-mannosyltransferase activity and is probably involved in the elongation of the mannan backbone of N-linked glycans on cell wall and periplasmic proteins.

GO - Molecular functioni

  • alpha-1,6-mannosyltransferase activity Source: UniProtKB
  • mannosyltransferase activity Source: SGD

GO - Biological processi

  • cell wall mannoprotein biosynthetic process Source: UniProtKB
  • mannosylation Source: GOC
  • protein N-linked glycosylation Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:YEL036C-MONOMER.

Protein family/group databases

CAZyiGT62. Glycosyltransferase Family 62.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan polymerase II complex ANP1 subunit
Short name:
M-Pol II subunit ANP1
Alternative name(s):
Aminonitrophenyl propanediol resistance protein
Gene namesi
Name:ANP1
Synonyms:GEM3
Ordered Locus Names:YEL036C
ORF Names:SYGP-ORF28
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL036C.
SGDiS000000762. ANP1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515CytoplasmicSequence analysisAdd
BLAST
Transmembranei16 – 2712Helical; Signal-anchor for type II membrane proteinAdd
BLAST
Topological domaini28 – 500473LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • alpha-1,6-mannosyltransferase complex Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • Golgi cis cisterna Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500Mannan polymerase II complex ANP1 subunitPRO_0000193668Add
BLAST

Proteomic databases

MaxQBiP32629.

Interactioni

Subunit structurei

Component of the M-Pol II complex composed of ANP1, MNN9, MNN10, MNN11 and HOC1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HOC1P471242EBI-2595,EBI-8430
MNN10P501083EBI-2595,EBI-11043
MNN11P469853EBI-2595,EBI-11052
MNN9P391076EBI-2595,EBI-11082

Protein-protein interaction databases

BioGridi36693. 509 interactions.
DIPiDIP-845N.
IntActiP32629. 19 interactions.
MINTiMINT-478883.

Structurei

3D structure databases

ProteinModelPortaliP32629.
SMRiP32629. Positions 62-357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi446 – 47328Gln-richAdd
BLAST

Sequence similaritiesi

Belongs to the ANP1/MMN9/VAN1 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000005201.
HOGENOMiHOG000167461.
InParanoidiP32629.
KOiK05533.
OMAiWKESEGG.
OrthoDBiEOG73V6VP.

Family and domain databases

InterProiIPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 3 hits.

Sequencei

Sequence statusi: Complete.

P32629-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYNNRKLSF NPTTVSIAGT LLTVFFLTRL VLSFFSISLF QLVTFQGIFK
60 70 80 90 100
PYVPDFKNTP SVEFYDLRNY QGNKDGWQQG DRILFCVPLR DASEHLPMFF
110 120 130 140 150
NHLNTMTYPH NLIDLSFLVS DSSDNTMGVL LSNLQMAQSQ QDKSKRFGNI
160 170 180 190 200
EIYEKDFGQI IGQSFSDRHG FGAQGPRRKL MARARNWLGS VALKPYHSWV
210 220 230 240 250
YWRDVDVETI PTTIMEDLMH HDKDVIVPNV WRPLPDWLGN IQPYDLNSWK
260 270 280 290 300
ESEGGLQLAD SLDEDAVIVE GYPEYATWRP HLAYMRDPNG NPEDEMELDG
310 320 330 340 350
IGGVSILAKA KVFRTGSHFP AFSFEKHAET EAFGRLSRRM NYNVIGLPHY
360 370 380 390 400
VIWHIYEPSS DDLKHMAWMA EEEKRKLEEE RIREFYNKIW EIGFEDVRDQ
410 420 430 440 450
WNEERDSILK NIDSTLNNKV TVDWSEEGDG SELVDSKGDF VSPNNQQQQQ
460 470 480 490 500
QQQQQQQQQQ QQQQQQQLDG NPQGKPLDDN DKNKKKHPKE VPLDFDPDRN
Length:500
Mass (Da):58,182
Last modified:February 1, 1995 - v3
Checksum:i845B395CE548CD14
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti220 – 2245HHDKD → QSGQGN in AAD13971 (PubMed:8411151).Curated
Sequence conflicti220 – 2245HHDKD → QSGQGN in AAA34426 (PubMed:8411151).Curated
Sequence conflicti313 – 3131F → L in AAD13971 (PubMed:8411151).Curated
Sequence conflicti313 – 3131F → L in AAA34426 (PubMed:8411151).Curated
Sequence conflicti313 – 3131F → L in AAB28440 (PubMed:8411151).Curated
Sequence conflicti313 – 3131F → L in AAA34937 (PubMed:8411151).Curated
Sequence conflicti472 – 50029PQGKP…DPDRN → RRGNLLMTTTRTRKNILKKF H in AAD13971 (PubMed:8411151).CuratedAdd
BLAST
Sequence conflicti472 – 50029PQGKP…DPDRN → RRGNLLMTTTRTRKNILKKF H in AAA34426 (PubMed:8411151).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S65964 Genomic DNA. Translation: AAD13971.1.
L22171 Genomic DNA. Translation: AAA34426.1.
S66114 mRNA. Translation: AAB28440.1.
L22173 Genomic DNA. Translation: AAA34937.1.
U18779 Genomic DNA. Translation: AAB65006.1.
BK006939 Genomic DNA. Translation: DAA07617.1.
PIRiS50508.
RefSeqiNP_010878.1. NM_001178851.1.

Genome annotation databases

EnsemblFungiiYEL036C; YEL036C; YEL036C.
GeneIDi856675.
KEGGisce:YEL036C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S65964 Genomic DNA. Translation: AAD13971.1.
L22171 Genomic DNA. Translation: AAA34426.1.
S66114 mRNA. Translation: AAB28440.1.
L22173 Genomic DNA. Translation: AAA34937.1.
U18779 Genomic DNA. Translation: AAB65006.1.
BK006939 Genomic DNA. Translation: DAA07617.1.
PIRiS50508.
RefSeqiNP_010878.1. NM_001178851.1.

3D structure databases

ProteinModelPortaliP32629.
SMRiP32629. Positions 62-357.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36693. 509 interactions.
DIPiDIP-845N.
IntActiP32629. 19 interactions.
MINTiMINT-478883.

Protein family/group databases

CAZyiGT62. Glycosyltransferase Family 62.

Proteomic databases

MaxQBiP32629.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYEL036C; YEL036C; YEL036C.
GeneIDi856675.
KEGGisce:YEL036C.

Organism-specific databases

EuPathDBiFungiDB:YEL036C.
SGDiS000000762. ANP1.

Phylogenomic databases

GeneTreeiENSGT00390000005201.
HOGENOMiHOG000167461.
InParanoidiP32629.
KOiK05533.
OMAiWKESEGG.
OrthoDBiEOG73V6VP.

Enzyme and pathway databases

BioCyciYEAST:YEL036C-MONOMER.

Miscellaneous databases

NextBioi982696.
PROiP32629.

Family and domain databases

InterProiIPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 3 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The gene clusters ARC and COR on chromosomes 5 and 10, respectively, of Saccharomyces cerevisiae share a common ancestry."
    Melnick L., Sherman F.
    J. Mol. Biol. 233:372-388(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: B-6441.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Novel membrane protein complexes for protein glycosylation in the yeast Golgi apparatus."
    Hashimoto H., Yoda K.
    Biochem. Biophys. Res. Commun. 241:682-686(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-10, SUBCELLULAR LOCATION, SUBUNIT.
  5. "Proteins in the early Golgi compartment of Saccharomyces cerevisiae immunoisolated by Sed5p."
    Cho J.-H., Noda Y., Yoda K.
    FEBS Lett. 469:151-154(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-5, SUBCELLULAR LOCATION.
  6. "The functioning of the yeast Golgi apparatus requires an ER protein encoded by ANP1, a member of a new family of genes affecting the secretory pathway."
    Chapman R.E., Munro S.
    EMBO J. 13:4896-4907(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha-1,6-mannosyltransferase activity."
    Jungmann J., Munro S.
    EMBO J. 17:423-434(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVITY OF M-POL II COMPLEX, SUBUNIT, SUBCELLULAR LOCATION.
  8. "Active recycling of yeast Golgi mannosyltransferase complexes through the endoplasmic reticulum."
    Todorow Z., Spang A., Carmack E., Yates J., Schekman R.
    Proc. Natl. Acad. Sci. U.S.A. 97:13643-13648(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiANP1_YEAST
AccessioniPrimary (citable) accession number: P32629
Secondary accession number(s): D3DLL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.