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Protein

UV excision repair protein RAD23

Gene

RAD23

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a central role both in proteasomal degradation of misfolded proteins and DNA repair. Central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol. Involved in DNA excision repair. May play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes.

GO - Molecular functioni

  • damaged DNA binding Source: SGD
  • proteasome binding Source: SGD
  • protein binding, bridging Source: SGD
  • ubiquitin binding Source: SGD

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • nucleotide-excision repair Source: InterPro
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
  • protein deglycosylation Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-30158-MONOMER.
ReactomeiR-SCE-5696394. DNA Damage Recognition in GG-NER.
R-SCE-5696395. Formation of Incision Complex in GG-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
UV excision repair protein RAD23
Gene namesi
Name:RAD23
Ordered Locus Names:YEL037C
ORF Names:SYGP-ORF29
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL037C.
SGDiS000000763. RAD23.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleotide-excision repair factor 2 complex Source: SGD
  • proteasome complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398UV excision repair protein RAD23PRO_0000114902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki49 – 49Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei94 – 941PhosphothreonineCombined sources
Modified residuei121 – 1211PhosphoserineCombined sources
Modified residuei139 – 1391PhosphothreonineCombined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP32628.
PRIDEiP32628.

PTM databases

iPTMnetiP32628.

Interactioni

Subunit structurei

Interacts directly with PNG1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PTH2P342225EBI-14668,EBI-2345448
RAD4P147363EBI-14668,EBI-14766
RPN1P387645EBI-14668,EBI-15913
RPT1P332993EBI-14668,EBI-13910
RPT3P332983EBI-14668,EBI-13905
RPT6Q019394EBI-14668,EBI-13914
SNF6P188882EBI-14668,EBI-17550
UBCP0CG484EBI-14668,EBI-3390054From a different organism.
UBI4P0CG634EBI-14668,EBI-7000452

GO - Molecular functioni

  • proteasome binding Source: SGD
  • protein binding, bridging Source: SGD
  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi36692. 192 interactions.
DIPiDIP-1548N.
IntActiP32628. 31 interactions.
MINTiMINT-404213.

Structurei

Secondary structure

1
398
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 74Combined sources
Beta strandi13 – 164Combined sources
Helixi24 – 3310Combined sources
Turni34 – 363Combined sources
Helixi39 – 413Combined sources
Beta strandi43 – 464Combined sources
Turni57 – 615Combined sources
Beta strandi67 – 715Combined sources
Helixi259 – 27012Combined sources
Helixi273 – 2753Combined sources
Helixi276 – 28611Combined sources
Helixi290 – 2967Combined sources
Helixi298 – 30710Combined sources
Helixi355 – 36511Combined sources
Turni366 – 3683Combined sources
Helixi371 – 38010Combined sources
Turni381 – 3833Combined sources
Helixi385 – 3928Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1X3WX-ray3.00B238-309[»]
1X3ZX-ray2.80B238-309[»]
2QSFX-ray2.35X230-398[»]
2QSGX-ray3.10X230-398[»]
2QSHX-ray2.81X230-398[»]
3ESWX-ray3.40B254-308[»]
3M62X-ray2.40B1-84[»]
4YIRX-ray3.05X230-398[»]
ProteinModelPortaliP32628.
SMRiP32628. Positions 2-398.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32628.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 7777Ubiquitin-likePROSITE-ProRule annotationAdd
BLAST
Domaini146 – 18641UBA 1PROSITE-ProRule annotationAdd
BLAST
Domaini355 – 39541UBA 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 UBA domains.PROSITE-ProRule annotation
Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00390000012078.
HOGENOMiHOG000172162.
InParanoidiP32628.
KOiK10839.
OMAiSATKQEN.
OrthoDBiEOG092C4GFV.

Family and domain databases

Gene3Di1.10.10.540. 1 hit.
InterProiIPR004806. Rad23.
IPR006636. STI1_HS-bd.
IPR015940. UBA.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
IPR015360. XPC-bd.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
PF00240. ubiquitin. 1 hit.
PF09280. XPC-binding. 1 hit.
[Graphical view]
PRINTSiPR01839. RAD23PROTEIN.
SMARTiSM00727. STI1. 1 hit.
SM00165. UBA. 2 hits.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF101238. SSF101238. 1 hit.
SSF46934. SSF46934. 2 hits.
SSF54236. SSF54236. 1 hit.
TIGRFAMsiTIGR00601. rad23. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32628-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSLTFKNFK KEKVPLDLEP SNTILETKTK LAQSISCEES QIKLIYSGKV
60 70 80 90 100
LQDSKTVSEC GLKDGDQVVF MVSQKKSTKT KVTEPPIAPE SATTPGRENS
110 120 130 140 150
TEASPSTDAS AAPAATAPEG SQPQEEQTAT TERTESASTP GFVVGTERNE
160 170 180 190 200
TIERIMEMGY QREEVERALR AAFNNPDRAV EYLLMGIPEN LRQPEPQQQT
210 220 230 240 250
AAAAEQPSTA ATTAEQPAED DLFAQAAQGG NASSGALGTT GGATDAAQGG
260 270 280 290 300
PPGSIGLTVE DLLSLRQVVS GNPEALAPLL ENISARYPQL REHIMANPEV
310 320 330 340 350
FVSMLLEAVG DNMQDVMEGA DDMVEGEDIE VTGEAAAAGL GQGEGEGSFQ
360 370 380 390
VDYTPEDDQA ISRLCELGFE RDLVIQVYFA CDKNEEAAAN ILFSDHAD
Length:398
Mass (Da):42,367
Last modified:October 1, 1993 - v1
Checksum:iB3F0436DAB60B833
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti277 – 2771A → R in AAA34935 (PubMed:8411151).Curated
Sequence conflicti277 – 2771A → R in AAA34938 (PubMed:8411151).Curated
Sequence conflicti277 – 2771A → R in AAD13972 (PubMed:8411151).Curated
Sequence conflicti277 – 2771A → R in AAB28441 (PubMed:8411151).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25428 Unassigned DNA. Translation: AAA16070.1.
L22172 Genomic DNA. Translation: AAA34935.1.
L22173 Genomic DNA. Translation: AAA34938.1.
S65964 Genomic DNA. Translation: AAD13972.1.
S66117 mRNA. Translation: AAB28441.1.
U18779 Genomic DNA. Translation: AAB65005.1.
AY693018 Genomic DNA. Translation: AAT93037.1.
BK006939 Genomic DNA. Translation: DAA07616.1.
PIRiS50507.
RefSeqiNP_010877.3. NM_001178852.3.

Genome annotation databases

EnsemblFungiiYEL037C; YEL037C; YEL037C.
GeneIDi856674.
KEGGisce:YEL037C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25428 Unassigned DNA. Translation: AAA16070.1.
L22172 Genomic DNA. Translation: AAA34935.1.
L22173 Genomic DNA. Translation: AAA34938.1.
S65964 Genomic DNA. Translation: AAD13972.1.
S66117 mRNA. Translation: AAB28441.1.
U18779 Genomic DNA. Translation: AAB65005.1.
AY693018 Genomic DNA. Translation: AAT93037.1.
BK006939 Genomic DNA. Translation: DAA07616.1.
PIRiS50507.
RefSeqiNP_010877.3. NM_001178852.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1X3WX-ray3.00B238-309[»]
1X3ZX-ray2.80B238-309[»]
2QSFX-ray2.35X230-398[»]
2QSGX-ray3.10X230-398[»]
2QSHX-ray2.81X230-398[»]
3ESWX-ray3.40B254-308[»]
3M62X-ray2.40B1-84[»]
4YIRX-ray3.05X230-398[»]
ProteinModelPortaliP32628.
SMRiP32628. Positions 2-398.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36692. 192 interactions.
DIPiDIP-1548N.
IntActiP32628. 31 interactions.
MINTiMINT-404213.

PTM databases

iPTMnetiP32628.

Proteomic databases

MaxQBiP32628.
PRIDEiP32628.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYEL037C; YEL037C; YEL037C.
GeneIDi856674.
KEGGisce:YEL037C.

Organism-specific databases

EuPathDBiFungiDB:YEL037C.
SGDiS000000763. RAD23.

Phylogenomic databases

GeneTreeiENSGT00390000012078.
HOGENOMiHOG000172162.
InParanoidiP32628.
KOiK10839.
OMAiSATKQEN.
OrthoDBiEOG092C4GFV.

Enzyme and pathway databases

BioCyciYEAST:G3O-30158-MONOMER.
ReactomeiR-SCE-5696394. DNA Damage Recognition in GG-NER.
R-SCE-5696395. Formation of Incision Complex in GG-NER.

Miscellaneous databases

EvolutionaryTraceiP32628.
PROiP32628.

Family and domain databases

Gene3Di1.10.10.540. 1 hit.
InterProiIPR004806. Rad23.
IPR006636. STI1_HS-bd.
IPR015940. UBA.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
IPR015360. XPC-bd.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
PF00240. ubiquitin. 1 hit.
PF09280. XPC-binding. 1 hit.
[Graphical view]
PRINTSiPR01839. RAD23PROTEIN.
SMARTiSM00727. STI1. 1 hit.
SM00165. UBA. 2 hits.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF101238. SSF101238. 1 hit.
SSF46934. SSF46934. 2 hits.
SSF54236. SSF54236. 1 hit.
TIGRFAMsiTIGR00601. rad23. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAD23_YEAST
AccessioniPrimary (citable) accession number: P32628
Secondary accession number(s): D3DLL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 7, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.