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P32626

- ENOPH_YEAST

UniProt

P32626 - ENOPH_YEAST

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Protein

Enolase-phosphatase E1

Gene
UTR4, YEL038W, SYGP-ORF20
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) By similarity.UniRule annotation

Catalytic activityi

5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi11 – 111Magnesium
Metal bindingi13 – 131Magnesium; via carbonyl oxygen
Binding sitei161 – 1611Substrate By similarity
Metal bindingi186 – 1861Magnesium

GO - Molecular functioni

  1. 2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity Source: UniProtKB-HAMAP
  2. 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity Source: UniProtKB-HAMAP
  3. acireductone synthase activity Source: SGD
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. L-methionine biosynthetic process from S-adenosylmethionine Source: UniProtKB-HAMAP
  3. L-methionine salvage from methylthioadenosine Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30159-MONOMER.
YEAST:MONOMER3O-175.
ReactomeiREACT_189018. Methionine salvage pathway.
UniPathwayiUPA00904; UER00876.
UPA00904; UER00877.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase-phosphatase E1 (EC:3.1.3.77)
Alternative name(s):
2,3-diketo-5-methylthio-1-phosphopentane phosphatase
Unknown transcript 4 protein
Gene namesi
Name:UTR4
Ordered Locus Names:YEL038W
ORF Names:SYGP-ORF20
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

SGDiS000000764. UTR4.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227Enolase-phosphatase E1UniRule annotationPRO_0000065746Add
BLAST

Proteomic databases

MaxQBiP32626.
PaxDbiP32626.
PeptideAtlasiP32626.

Expressioni

Gene expression databases

GenevestigatoriP32626.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

BioGridi36691. 24 interactions.
DIPiDIP-5487N.
MINTiMINT-513487.
STRINGi4932.YEL038W.

Structurei

Secondary structure

1
227
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 105
Turni14 – 163
Helixi21 – 244
Helixi26 – 3914
Helixi46 – 527
Helixi59 – 7113
Helixi77 – 9216
Helixi102 – 1109
Beta strandi114 – 1174
Helixi122 – 1309
Helixi146 – 1483
Beta strandi151 – 1533
Helixi155 – 1584
Helixi164 – 17411
Helixi178 – 1803
Beta strandi181 – 1866
Helixi188 – 1958
Turni196 – 1983
Beta strandi200 – 2045
Beta strandi220 – 2223

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2G80X-ray2.28A/B/C/D1-227[»]
ProteinModelPortaliP32626.
SMRiP32626. Positions 3-227.

Miscellaneous databases

EvolutionaryTraceiP32626.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni118 – 1192Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG4229.
GeneTreeiENSGT00440000039914.
HOGENOMiHOG000237286.
KOiK09880.
OMAiLLFGHTN.
OrthoDBiEOG75B8GZ.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
HAMAPiMF_03117. Salvage_MtnC_euk.
InterProiIPR023943. Enolase-ppase_E1.
IPR027511. ENOPH1_eukaryotes.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF13419. HAD_2. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01691. enolase-ppase. 1 hit.

Sequencei

Sequence statusi: Complete.

P32626-1 [UniParc]FASTAAdd to Basket

« Hide

MGDNYSTYLL DIEGTVCPIS FVKETLFPYF TNKVPQLVQQ DTRDSPVSNI    50
LSQFHIDNKE QLQAHILELV AKDVKDPILK QLQGYVWAHG YESGQIKAPV 100
YADAIDFIKR KKRVFIYSSG SVKAQKLLFG YVQDPNAPAH DSLDLNSYID 150
GYFDINTSGK KTETQSYANI LRDIGAKASE VLFLSDNPLE LDAAAGVGIA 200
TGLASRPGNA PVPDGQKYQV YKNFETL 227
Length:227
Mass (Da):25,187
Last modified:June 16, 2009 - v2
Checksum:i147305E847F765DB
GO

Sequence cautioni

The sequence AAA34939.1 differs from that shown. Reason: Frameshift at positions 139 and 216.
The sequence AAB28443.1 differs from that shown. Reason: Frameshift at positions 139 and 216.
The sequence AAD13973.1 differs from that shown. Reason: Frameshift at positions 139 and 216.
The sequence AAA34939.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence AAB28443.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence AAB65004.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence AAD13973.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 572ID → MH in AAA34939. 1 Publication
Sequence conflicti56 – 572ID → MH in AAD13973. 1 Publication
Sequence conflicti56 – 572ID → MH in AAB28443. 1 Publication
Sequence conflicti179 – 1791S → T in AAA34939. 1 Publication
Sequence conflicti179 – 1791S → T in AAD13973. 1 Publication
Sequence conflicti179 – 1791S → T in AAB28443. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L22173 Genomic DNA. Translation: AAA34939.1. Sequence problems.
S65964 Genomic DNA. Translation: AAD13973.1. Sequence problems.
S66121 mRNA. Translation: AAB28443.1. Sequence problems.
U18779 Genomic DNA. Translation: AAB65004.1. Different initiation.
BK006939 Genomic DNA. Translation: DAA07615.1.
PIRiS30843.
RefSeqiNP_010876.2. NM_001178853.1.

Genome annotation databases

EnsemblFungiiYEL038W; YEL038W; YEL038W.
GeneIDi856673.
KEGGisce:YEL038W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L22173 Genomic DNA. Translation: AAA34939.1 . Sequence problems.
S65964 Genomic DNA. Translation: AAD13973.1 . Sequence problems.
S66121 mRNA. Translation: AAB28443.1 . Sequence problems.
U18779 Genomic DNA. Translation: AAB65004.1 . Different initiation.
BK006939 Genomic DNA. Translation: DAA07615.1 .
PIRi S30843.
RefSeqi NP_010876.2. NM_001178853.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2G80 X-ray 2.28 A/B/C/D 1-227 [» ]
ProteinModelPortali P32626.
SMRi P32626. Positions 3-227.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36691. 24 interactions.
DIPi DIP-5487N.
MINTi MINT-513487.
STRINGi 4932.YEL038W.

Proteomic databases

MaxQBi P32626.
PaxDbi P32626.
PeptideAtlasi P32626.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YEL038W ; YEL038W ; YEL038W .
GeneIDi 856673.
KEGGi sce:YEL038W.

Organism-specific databases

SGDi S000000764. UTR4.

Phylogenomic databases

eggNOGi COG4229.
GeneTreei ENSGT00440000039914.
HOGENOMi HOG000237286.
KOi K09880.
OMAi LLFGHTN.
OrthoDBi EOG75B8GZ.

Enzyme and pathway databases

UniPathwayi UPA00904 ; UER00876 .
UPA00904 ; UER00877 .
BioCyci YEAST:G3O-30159-MONOMER.
YEAST:MONOMER3O-175.
Reactomei REACT_189018. Methionine salvage pathway.

Miscellaneous databases

EvolutionaryTracei P32626.
NextBioi 982690.
PROi P32626.

Gene expression databases

Genevestigatori P32626.

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
HAMAPi MF_03117. Salvage_MtnC_euk.
InterProi IPR023943. Enolase-ppase_E1.
IPR027511. ENOPH1_eukaryotes.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF13419. HAD_2. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR01691. enolase-ppase. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The gene clusters ARC and COR on chromosomes 5 and 10, respectively, of Saccharomyces cerevisiae share a common ancestry."
    Melnick L., Sherman F.
    J. Mol. Biol. 233:372-388(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: B-6441.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Crystal structure of UTR4 protein (unknown transcript 4 protein) (YEL038w) from Saccharomyces cerevisiae at 2.28 A resolution."
    Joint center for structural genomics (JCSG)
    Submitted (MAR-2006) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.28 ANGSTROMS) IN COMPLEX WITH MAGNESIUM.

Entry informationi

Entry nameiENOPH_YEAST
AccessioniPrimary (citable) accession number: P32626
Secondary accession number(s): D3DLL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: June 16, 2009
Last modified: September 3, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2850 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

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