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Protein

Probable glycosidase CRH2

Gene

UTR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable glycosidase that plays a role in cell wall architecture. Required for the transfer of chitin to 1,6-beta-glucan in the cell wall.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei166NucleophileBy similarity1
Active sitei170Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cell wall chitin metabolic process Source: SGD
  • fungal-type cell wall organization Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-30161-MONOMER.

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glycosidase CRH2 (EC:3.2.-.-)
Alternative name(s):
Congo red hypersensitive protein 2
Unknown transcript 2 protein
Gene namesi
Name:UTR2
Synonyms:CRH2
Ordered Locus Names:YEL040W
ORF Names:SYGP-ORF18
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL040W.
SGDiS000000766. UTR2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000006574524 – 445Probable glycosidase CRH2Add BLAST422
PropeptideiPRO_0000232722446 – 467Removed in mature formSequence analysisAdd BLAST22

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi28N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi96N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi190N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi196N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi233N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi237N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi261N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi297N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi310N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi445GPI-anchor amidated asparagineSequence analysis1

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiP32623.
PRIDEiP32623.

Interactioni

Protein-protein interaction databases

BioGridi36689. 88 interactors.
IntActiP32623. 6 interactors.
MINTiMINT-2785828.
STRINGi4932.YEL040W.

Structurei

3D structure databases

ProteinModelPortaliP32623.
SMRiP32623.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 280GH16PROSITE-ProRule annotationAdd BLAST218

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi354 – 442Ser-richAdd BLAST89

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00610000086657.
HOGENOMiHOG000184016.
InParanoidiP32623.
OMAiMWYGNVK.
OrthoDBiEOG092C3MQ4.

Family and domain databases

InterProiView protein in InterPro
IPR013320. ConA-like_dom.
IPR000757. GH16.
IPR017168. Glyco_hydro_16_CRH1_prd.
PfamiView protein in Pfam
PF00722. Glyco_hydro_16. 1 hit.
PIRSFiPIRSF037299. Glycosidase_CRH1_prd. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiView protein in PROSITE
PS51762. GH16_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32623-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIVNSWLIC LVSIFSFVVR VEAATFCNAT QACPEDKPCC SQYGECGTGQ
60 70 80 90 100
YCLNNCDVRY SFSHDSCMPV PICKSSSTKF KDYSSKLGNA NTFLGNVSEA
110 120 130 140 150
DWLYTGDVLD YDDEESLILA MPKNSGGTVL SSTRAVWYGK VSARIKTSHL
160 170 180 190 200
AGVVTGFILY SGAGDELDYE FVGADLETAQ TNFYWESVLN YTNSANISTT
210 220 230 240 250
DTFENYHTYE LDWHEDYVTW SIDGVVGRTL YKNETYNATT QKYQYPQTPS
260 270 280 290 300
KVDISIWPGG NSTNAPGTIA WSGGEINWDA SDISNPGYYY AIVNEVNITC
310 320 330 340 350
YDPPSDTKKN GTSAYVYTSS SEFLAKDIAI TDDEVMMDSD EGSGLDPHKG
360 370 380 390 400
ATTSSTQKSS SSTATSSSKT SSDHSSSTKK SSKTSSTASS SSSSSSSSSS
410 420 430 440 450
SSSTATKNGD KVVSSVSSSV TSQTQTTSSV SGSASSSTSS MSGNNAGANV
460
AANWRLTVLC VILGYVL
Length:467
Mass (Da):49,906
Last modified:April 18, 2006 - v3
Checksum:iB6AE762B5489AA3A
GO

Sequence cautioni

The sequence AAB28444 differs from that shown. Reason: Frameshift at position 120.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti130L → V in AAA34941 (PubMed:8411151).Curated1
Sequence conflicti130L → V in AAD13975 (PubMed:8411151).Curated1
Sequence conflicti130L → V in AAB28444 (PubMed:8411151).Curated1
Sequence conflicti291A → R in AAA34941 (PubMed:8411151).Curated1
Sequence conflicti291A → R in AAD13975 (PubMed:8411151).Curated1
Sequence conflicti291A → R in AAB28444 (PubMed:8411151).Curated1
Sequence conflicti354S → C in AAA34941 (PubMed:8411151).Curated1
Sequence conflicti354S → C in AAD13975 (PubMed:8411151).Curated1
Sequence conflicti354S → C in AAB28444 (PubMed:8411151).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18779 Genomic DNA. Translation: AAB65002.1.
AY693014 Genomic DNA. Translation: AAT93033.1.
L22173 Genomic DNA. Translation: AAA34941.1. Frameshift.
S65964 Genomic DNA. Translation: AAD13975.1. Frameshift.
S66130 mRNA. Translation: AAB28444.1. Frameshift.
BK006939 Genomic DNA. Translation: DAA07613.1.
PIRiS30839.
RefSeqiNP_010874.3. NM_001178855.3.

Genome annotation databases

EnsemblFungiiYEL040W; YEL040W; YEL040W.
GeneIDi856671.
KEGGisce:YEL040W.

Similar proteinsi

Entry informationi

Entry nameiCRH2_YEAST
AccessioniPrimary (citable) accession number: P32623
Secondary accession number(s): D3DLK9, Q6B1R6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 18, 2006
Last modified: September 27, 2017
This is version 147 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names