Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P32623

- CRH2_YEAST

UniProt

P32623 - CRH2_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Probable glycosidase CRH2

Gene

UTR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Probable glycosidase that plays a role in cell wall architecture. Required for the transfer of chitin to 1,6-beta-glucan in the cell wall.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei166 – 1661NucleophileBy similarity
Active sitei170 – 1701Proton donorBy similarity

GO - Molecular functioni

  1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
  2. transferase activity, transferring glycosyl groups Source: SGD

GO - Biological processi

  1. cell wall chitin metabolic process Source: SGD
  2. fungal-type cell wall organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-30161-MONOMER.

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glycosidase CRH2 (EC:3.2.-.-)
Alternative name(s):
Congo red hypersensitive protein 2
Unknown transcript 2 protein
Gene namesi
Name:UTR2
Synonyms:CRH2
Ordered Locus Names:YEL040W
ORF Names:SYGP-ORF18
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYEL040w.
SGDiS000000766. UTR2.

Subcellular locationi

Secretedcell wall. Membrane; Lipid-anchorGPI-anchor
Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP), localized particularly in chitin-rich areas. Localizes to sites of polarized growth. Found at the incipient bud site, as a ring at the bud neck as the bud grows, and in the septum at the time of cytokinesis. Redistributes uniformly over the cell cortex upon heat stress.

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. cellular bud neck septin ring Source: SGD
  3. extracellular region Source: UniProtKB-KW
  4. fungal-type cell wall Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 445422Probable glycosidase CRH2PRO_0000065745Add
BLAST
Propeptidei446 – 46722Removed in mature formSequence AnalysisPRO_0000232722Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi28 – 281N-linked (GlcNAc...)Sequence Analysis
Glycosylationi96 – 961N-linked (GlcNAc...)Sequence Analysis
Glycosylationi190 – 1901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi196 – 1961N-linked (GlcNAc...)Sequence Analysis
Glycosylationi233 – 2331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi237 – 2371N-linked (GlcNAc...)Sequence Analysis
Glycosylationi261 – 2611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi310 – 3101N-linked (GlcNAc...)Sequence Analysis
Lipidationi445 – 4451GPI-anchor amidated asparagineSequence Analysis

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiP32623.
PaxDbiP32623.
PeptideAtlasiP32623.

Expressioni

Gene expression databases

GenevestigatoriP32623.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
AGP1P253761EBI-2098544,EBI-2357

Protein-protein interaction databases

BioGridi36689. 77 interactions.
MINTiMINT-2785828.
STRINGi4932.YEL040W.

Structurei

3D structure databases

ProteinModelPortaliP32623.
SMRiP32623. Positions 132-260.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi354 – 44289Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2273.
GeneTreeiENSGT00610000086657.
HOGENOMiHOG000184016.
InParanoidiP32623.
OMAiLGGCDIR.
OrthoDBiEOG7TN02H.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR017168. Glyco_hydro_16_CRH1_prd.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PIRSFiPIRSF037299. Glycosidase_CRH1_prd. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32623-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAIVNSWLIC LVSIFSFVVR VEAATFCNAT QACPEDKPCC SQYGECGTGQ
60 70 80 90 100
YCLNNCDVRY SFSHDSCMPV PICKSSSTKF KDYSSKLGNA NTFLGNVSEA
110 120 130 140 150
DWLYTGDVLD YDDEESLILA MPKNSGGTVL SSTRAVWYGK VSARIKTSHL
160 170 180 190 200
AGVVTGFILY SGAGDELDYE FVGADLETAQ TNFYWESVLN YTNSANISTT
210 220 230 240 250
DTFENYHTYE LDWHEDYVTW SIDGVVGRTL YKNETYNATT QKYQYPQTPS
260 270 280 290 300
KVDISIWPGG NSTNAPGTIA WSGGEINWDA SDISNPGYYY AIVNEVNITC
310 320 330 340 350
YDPPSDTKKN GTSAYVYTSS SEFLAKDIAI TDDEVMMDSD EGSGLDPHKG
360 370 380 390 400
ATTSSTQKSS SSTATSSSKT SSDHSSSTKK SSKTSSTASS SSSSSSSSSS
410 420 430 440 450
SSSTATKNGD KVVSSVSSSV TSQTQTTSSV SGSASSSTSS MSGNNAGANV
460
AANWRLTVLC VILGYVL
Length:467
Mass (Da):49,906
Last modified:April 18, 2006 - v3
Checksum:iB6AE762B5489AA3A
GO

Sequence cautioni

The sequence AAB28444.1 differs from that shown. Reason: Frameshift at position 120. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti130 – 1301L → V in AAA34941. (PubMed:8411151)Curated
Sequence conflicti130 – 1301L → V in AAD13975. (PubMed:8411151)Curated
Sequence conflicti130 – 1301L → V in AAB28444. (PubMed:8411151)Curated
Sequence conflicti291 – 2911A → R in AAA34941. (PubMed:8411151)Curated
Sequence conflicti291 – 2911A → R in AAD13975. (PubMed:8411151)Curated
Sequence conflicti291 – 2911A → R in AAB28444. (PubMed:8411151)Curated
Sequence conflicti354 – 3541S → C in AAA34941. (PubMed:8411151)Curated
Sequence conflicti354 – 3541S → C in AAD13975. (PubMed:8411151)Curated
Sequence conflicti354 – 3541S → C in AAB28444. (PubMed:8411151)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18779 Genomic DNA. Translation: AAB65002.1.
AY693014 Genomic DNA. Translation: AAT93033.1.
L22173 Genomic DNA. Translation: AAA34941.1. Frameshift.
S65964 Genomic DNA. Translation: AAD13975.1. Frameshift.
S66130 mRNA. Translation: AAB28444.1. Frameshift.
BK006939 Genomic DNA. Translation: DAA07613.1.
PIRiS30839.
RefSeqiNP_010874.3. NM_001178855.3.

Genome annotation databases

EnsemblFungiiYEL040W; YEL040W; YEL040W.
GeneIDi856671.
KEGGisce:YEL040W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18779 Genomic DNA. Translation: AAB65002.1 .
AY693014 Genomic DNA. Translation: AAT93033.1 .
L22173 Genomic DNA. Translation: AAA34941.1 . Frameshift.
S65964 Genomic DNA. Translation: AAD13975.1 . Frameshift.
S66130 mRNA. Translation: AAB28444.1 . Frameshift.
BK006939 Genomic DNA. Translation: DAA07613.1 .
PIRi S30839.
RefSeqi NP_010874.3. NM_001178855.3.

3D structure databases

ProteinModelPortali P32623.
SMRi P32623. Positions 132-260.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36689. 77 interactions.
MINTi MINT-2785828.
STRINGi 4932.YEL040W.

Protein family/group databases

CAZyi CBM18. Carbohydrate-Binding Module Family 18.
GH16. Glycoside Hydrolase Family 16.

Proteomic databases

MaxQBi P32623.
PaxDbi P32623.
PeptideAtlasi P32623.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YEL040W ; YEL040W ; YEL040W .
GeneIDi 856671.
KEGGi sce:YEL040W.

Organism-specific databases

CYGDi YEL040w.
SGDi S000000766. UTR2.

Phylogenomic databases

eggNOGi COG2273.
GeneTreei ENSGT00610000086657.
HOGENOMi HOG000184016.
InParanoidi P32623.
OMAi LGGCDIR.
OrthoDBi EOG7TN02H.

Enzyme and pathway databases

BioCyci YEAST:G3O-30161-MONOMER.

Miscellaneous databases

NextBioi 982684.

Gene expression databases

Genevestigatori P32623.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR017168. Glyco_hydro_16_CRH1_prd.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
[Graphical view ]
PIRSFi PIRSF037299. Glycosidase_CRH1_prd. 1 hit.
SUPFAMi SSF49899. SSF49899. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "The gene clusters ARC and COR on chromosomes 5 and 10, respectively, of Saccharomyces cerevisiae share a common ancestry."
    Melnick L., Sherman F.
    J. Mol. Biol. 233:372-388(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 76-467.
    Strain: B-6441.
  5. "Screening for glycosylphosphatidylinositol (GPI)-dependent cell wall proteins in Saccharomyces cerevisiae."
    Hamada K., Fukuchi S., Arisawa M., Baba M., Kitada K.
    Mol. Gen. Genet. 258:53-59(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. "A novel family of cell wall-related proteins regulated differently during the yeast life cycle."
    Rodriguez-Pena J.M., Cid V.J., Arroyo J., Nombela C.
    Mol. Cell. Biol. 20:3245-3255(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Mechanisms for targeting of the Saccharomyces cerevisiae GPI-anchored cell wall protein Crh2p to polarised growth sites."
    Rodriguez-Pena J.M., Rodriguez C., Alvarez A., Nombela C., Arroyo J.
    J. Cell Sci. 115:2549-2558(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages."
    Yin Q.Y., de Groot P.W.J., Dekker H.L., de Jong L., Klis F.M., de Koster C.G.
    J. Biol. Chem. 280:20894-20901(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  9. "Crh1p and Crh2p are required for the cross-linking of chitin to beta(1-6)glucan in the Saccharomyces cerevisiae cell wall."
    Cabib E., Blanco N., Grau C., Rodriguez-Pena J.M., Arroyo J.
    Mol. Microbiol. 63:921-935(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION BY HEAT STRESS.

Entry informationi

Entry nameiCRH2_YEAST
AccessioniPrimary (citable) accession number: P32623
Secondary accession number(s): D3DLK9, Q6B1R6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 18, 2006
Last modified: November 26, 2014
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3