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P32623

- CRH2_YEAST

UniProt

P32623 - CRH2_YEAST

Protein

Probable glycosidase CRH2

Gene

UTR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 3 (18 Apr 2006)
      Previous versions | rss
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    Functioni

    Probable glycosidase that plays a role in cell wall architecture. Required for the transfer of chitin to 1,6-beta-glucan in the cell wall.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei166 – 1661NucleophileBy similarity
    Active sitei170 – 1701Proton donorBy similarity

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
    2. transferase activity, transferring glycosyl groups Source: SGD

    GO - Biological processi

    1. cell wall chitin metabolic process Source: SGD
    2. fungal-type cell wall organization Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30161-MONOMER.

    Protein family/group databases

    CAZyiCBM18. Carbohydrate-Binding Module Family 18.
    GH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable glycosidase CRH2 (EC:3.2.-.-)
    Alternative name(s):
    Congo red hypersensitive protein 2
    Unknown transcript 2 protein
    Gene namesi
    Name:UTR2
    Synonyms:CRH2
    Ordered Locus Names:YEL040W
    ORF Names:SYGP-ORF18
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    CYGDiYEL040w.
    SGDiS000000766. UTR2.

    Subcellular locationi

    Secretedcell wall. Membrane; Lipid-anchorGPI-anchor
    Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP), localized particularly in chitin-rich areas. Localizes to sites of polarized growth. Found at the incipient bud site, as a ring at the bud neck as the bud grows, and in the septum at the time of cytokinesis. Redistributes uniformly over the cell cortex upon heat stress.

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. cellular bud neck septin ring Source: SGD
    3. extracellular region Source: UniProtKB-KW
    4. fungal-type cell wall Source: SGD

    Keywords - Cellular componenti

    Cell wall, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 445422Probable glycosidase CRH2PRO_0000065745Add
    BLAST
    Propeptidei446 – 46722Removed in mature formSequence AnalysisPRO_0000232722Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi28 – 281N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi96 – 961N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi190 – 1901N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi196 – 1961N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi233 – 2331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi237 – 2371N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi261 – 2611N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi310 – 3101N-linked (GlcNAc...)Sequence Analysis
    Lipidationi445 – 4451GPI-anchor amidated asparagineSequence Analysis

    Post-translational modificationi

    The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    MaxQBiP32623.
    PaxDbiP32623.
    PeptideAtlasiP32623.

    Expressioni

    Gene expression databases

    GenevestigatoriP32623.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AGP1P253761EBI-2098544,EBI-2357

    Protein-protein interaction databases

    BioGridi36689. 76 interactions.
    MINTiMINT-2785828.
    STRINGi4932.YEL040W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32623.
    SMRiP32623. Positions 132-260.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi354 – 44289Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 16 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2273.
    GeneTreeiENSGT00610000086657.
    HOGENOMiHOG000184016.
    OMAiLGGCDIR.
    OrthoDBiEOG7TN02H.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR017168. Glyco_hydro_16_CRH1_prd.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037299. Glycosidase_CRH1_prd. 1 hit.
    SUPFAMiSSF49899. SSF49899. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32623-1 [UniParc]FASTAAdd to Basket

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    MAIVNSWLIC LVSIFSFVVR VEAATFCNAT QACPEDKPCC SQYGECGTGQ    50
    YCLNNCDVRY SFSHDSCMPV PICKSSSTKF KDYSSKLGNA NTFLGNVSEA 100
    DWLYTGDVLD YDDEESLILA MPKNSGGTVL SSTRAVWYGK VSARIKTSHL 150
    AGVVTGFILY SGAGDELDYE FVGADLETAQ TNFYWESVLN YTNSANISTT 200
    DTFENYHTYE LDWHEDYVTW SIDGVVGRTL YKNETYNATT QKYQYPQTPS 250
    KVDISIWPGG NSTNAPGTIA WSGGEINWDA SDISNPGYYY AIVNEVNITC 300
    YDPPSDTKKN GTSAYVYTSS SEFLAKDIAI TDDEVMMDSD EGSGLDPHKG 350
    ATTSSTQKSS SSTATSSSKT SSDHSSSTKK SSKTSSTASS SSSSSSSSSS 400
    SSSTATKNGD KVVSSVSSSV TSQTQTTSSV SGSASSSTSS MSGNNAGANV 450
    AANWRLTVLC VILGYVL 467
    Length:467
    Mass (Da):49,906
    Last modified:April 18, 2006 - v3
    Checksum:iB6AE762B5489AA3A
    GO

    Sequence cautioni

    The sequence AAB28444.1 differs from that shown. Reason: Frameshift at position 120.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti130 – 1301L → V in AAA34941. (PubMed:8411151)Curated
    Sequence conflicti130 – 1301L → V in AAD13975. (PubMed:8411151)Curated
    Sequence conflicti130 – 1301L → V in AAB28444. (PubMed:8411151)Curated
    Sequence conflicti291 – 2911A → R in AAA34941. (PubMed:8411151)Curated
    Sequence conflicti291 – 2911A → R in AAD13975. (PubMed:8411151)Curated
    Sequence conflicti291 – 2911A → R in AAB28444. (PubMed:8411151)Curated
    Sequence conflicti354 – 3541S → C in AAA34941. (PubMed:8411151)Curated
    Sequence conflicti354 – 3541S → C in AAD13975. (PubMed:8411151)Curated
    Sequence conflicti354 – 3541S → C in AAB28444. (PubMed:8411151)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18779 Genomic DNA. Translation: AAB65002.1.
    AY693014 Genomic DNA. Translation: AAT93033.1.
    L22173 Genomic DNA. Translation: AAA34941.1. Frameshift.
    S65964 Genomic DNA. Translation: AAD13975.1. Frameshift.
    S66130 mRNA. Translation: AAB28444.1. Frameshift.
    BK006939 Genomic DNA. Translation: DAA07613.1.
    PIRiS30839.
    RefSeqiNP_010874.3. NM_001178855.3.

    Genome annotation databases

    EnsemblFungiiYEL040W; YEL040W; YEL040W.
    GeneIDi856671.
    KEGGisce:YEL040W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18779 Genomic DNA. Translation: AAB65002.1 .
    AY693014 Genomic DNA. Translation: AAT93033.1 .
    L22173 Genomic DNA. Translation: AAA34941.1 . Frameshift.
    S65964 Genomic DNA. Translation: AAD13975.1 . Frameshift.
    S66130 mRNA. Translation: AAB28444.1 . Frameshift.
    BK006939 Genomic DNA. Translation: DAA07613.1 .
    PIRi S30839.
    RefSeqi NP_010874.3. NM_001178855.3.

    3D structure databases

    ProteinModelPortali P32623.
    SMRi P32623. Positions 132-260.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36689. 76 interactions.
    MINTi MINT-2785828.
    STRINGi 4932.YEL040W.

    Protein family/group databases

    CAZyi CBM18. Carbohydrate-Binding Module Family 18.
    GH16. Glycoside Hydrolase Family 16.

    Proteomic databases

    MaxQBi P32623.
    PaxDbi P32623.
    PeptideAtlasi P32623.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YEL040W ; YEL040W ; YEL040W .
    GeneIDi 856671.
    KEGGi sce:YEL040W.

    Organism-specific databases

    CYGDi YEL040w.
    SGDi S000000766. UTR2.

    Phylogenomic databases

    eggNOGi COG2273.
    GeneTreei ENSGT00610000086657.
    HOGENOMi HOG000184016.
    OMAi LGGCDIR.
    OrthoDBi EOG7TN02H.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30161-MONOMER.

    Miscellaneous databases

    NextBioi 982684.

    Gene expression databases

    Genevestigatori P32623.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR017168. Glyco_hydro_16_CRH1_prd.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037299. Glycosidase_CRH1_prd. 1 hit.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "The gene clusters ARC and COR on chromosomes 5 and 10, respectively, of Saccharomyces cerevisiae share a common ancestry."
      Melnick L., Sherman F.
      J. Mol. Biol. 233:372-388(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 76-467.
      Strain: B-6441.
    5. "Screening for glycosylphosphatidylinositol (GPI)-dependent cell wall proteins in Saccharomyces cerevisiae."
      Hamada K., Fukuchi S., Arisawa M., Baba M., Kitada K.
      Mol. Gen. Genet. 258:53-59(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    6. "A novel family of cell wall-related proteins regulated differently during the yeast life cycle."
      Rodriguez-Pena J.M., Cid V.J., Arroyo J., Nombela C.
      Mol. Cell. Biol. 20:3245-3255(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "Mechanisms for targeting of the Saccharomyces cerevisiae GPI-anchored cell wall protein Crh2p to polarised growth sites."
      Rodriguez-Pena J.M., Rodriguez C., Alvarez A., Nombela C., Arroyo J.
      J. Cell Sci. 115:2549-2558(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    8. "Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of proteins covalently attached via glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages."
      Yin Q.Y., de Groot P.W.J., Dekker H.L., de Jong L., Klis F.M., de Koster C.G.
      J. Biol. Chem. 280:20894-20901(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    9. "Crh1p and Crh2p are required for the cross-linking of chitin to beta(1-6)glucan in the Saccharomyces cerevisiae cell wall."
      Cabib E., Blanco N., Grau C., Rodriguez-Pena J.M., Arroyo J.
      Mol. Microbiol. 63:921-935(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION BY HEAT STRESS.

    Entry informationi

    Entry nameiCRH2_YEAST
    AccessioniPrimary (citable) accession number: P32623
    Secondary accession number(s): D3DLK9, Q6B1R6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: April 18, 2006
    Last modified: October 1, 2014
    This is version 123 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3