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Protein

Probable glycosidase CRH2

Gene

UTR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable glycosidase that plays a role in cell wall architecture. Required for the transfer of chitin to 1,6-beta-glucan in the cell wall.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei166NucleophileBy similarity1
Active sitei170Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cell wall chitin metabolic process Source: SGD
  • fungal-type cell wall organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-30161-MONOMER.

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glycosidase CRH2 (EC:3.2.-.-)
Alternative name(s):
Congo red hypersensitive protein 2
Unknown transcript 2 protein
Gene namesi
Name:UTR2
Synonyms:CRH2
Ordered Locus Names:YEL040W
ORF Names:SYGP-ORF18
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL040W.
SGDiS000000766. UTR2.

Subcellular locationi

  • Secretedcell wall
  • Membrane; Lipid-anchorGPI-anchor

  • Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP), localized particularly in chitin-rich areas. Localizes to sites of polarized growth. Found at the incipient bud site, as a ring at the bud neck as the bud grows, and in the septum at the time of cytokinesis. Redistributes uniformly over the cell cortex upon heat stress.

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • cellular bud neck septin ring Source: SGD
  • extracellular region Source: UniProtKB-KW
  • fungal-type cell wall Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000006574524 – 445Probable glycosidase CRH2Add BLAST422
PropeptideiPRO_0000232722446 – 467Removed in mature formSequence analysisAdd BLAST22

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi28N-linked (GlcNAc...)Sequence analysis1
Glycosylationi96N-linked (GlcNAc...)Sequence analysis1
Glycosylationi190N-linked (GlcNAc...)Sequence analysis1
Glycosylationi196N-linked (GlcNAc...)Sequence analysis1
Glycosylationi233N-linked (GlcNAc...)Sequence analysis1
Glycosylationi237N-linked (GlcNAc...)Sequence analysis1
Glycosylationi261N-linked (GlcNAc...)Sequence analysis1
Glycosylationi297N-linked (GlcNAc...)Sequence analysis1
Glycosylationi310N-linked (GlcNAc...)Sequence analysis1
Lipidationi445GPI-anchor amidated asparagineSequence analysis1

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiP32623.
PRIDEiP32623.

Interactioni

Protein-protein interaction databases

BioGridi36689. 77 interactors.
MINTiMINT-2785828.

Structurei

3D structure databases

ProteinModelPortaliP32623.
SMRiP32623.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 280GH16PROSITE-ProRule annotationAdd BLAST218

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi354 – 442Ser-richAdd BLAST89

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated
Contains 1 GH16 (glycosyl hydrolase family 16) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00610000086657.
HOGENOMiHOG000184016.
InParanoidiP32623.
OMAiLGGCDIR.
OrthoDBiEOG092C3MQ4.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. GH16.
IPR017168. Glyco_hydro_16_CRH1_prd.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PIRSFiPIRSF037299. Glycosidase_CRH1_prd. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51762. GH16_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32623-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIVNSWLIC LVSIFSFVVR VEAATFCNAT QACPEDKPCC SQYGECGTGQ
60 70 80 90 100
YCLNNCDVRY SFSHDSCMPV PICKSSSTKF KDYSSKLGNA NTFLGNVSEA
110 120 130 140 150
DWLYTGDVLD YDDEESLILA MPKNSGGTVL SSTRAVWYGK VSARIKTSHL
160 170 180 190 200
AGVVTGFILY SGAGDELDYE FVGADLETAQ TNFYWESVLN YTNSANISTT
210 220 230 240 250
DTFENYHTYE LDWHEDYVTW SIDGVVGRTL YKNETYNATT QKYQYPQTPS
260 270 280 290 300
KVDISIWPGG NSTNAPGTIA WSGGEINWDA SDISNPGYYY AIVNEVNITC
310 320 330 340 350
YDPPSDTKKN GTSAYVYTSS SEFLAKDIAI TDDEVMMDSD EGSGLDPHKG
360 370 380 390 400
ATTSSTQKSS SSTATSSSKT SSDHSSSTKK SSKTSSTASS SSSSSSSSSS
410 420 430 440 450
SSSTATKNGD KVVSSVSSSV TSQTQTTSSV SGSASSSTSS MSGNNAGANV
460
AANWRLTVLC VILGYVL
Length:467
Mass (Da):49,906
Last modified:April 18, 2006 - v3
Checksum:iB6AE762B5489AA3A
GO

Sequence cautioni

The sequence AAB28444 differs from that shown. Reason: Frameshift at position 120.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti130L → V in AAA34941 (PubMed:8411151).Curated1
Sequence conflicti130L → V in AAD13975 (PubMed:8411151).Curated1
Sequence conflicti130L → V in AAB28444 (PubMed:8411151).Curated1
Sequence conflicti291A → R in AAA34941 (PubMed:8411151).Curated1
Sequence conflicti291A → R in AAD13975 (PubMed:8411151).Curated1
Sequence conflicti291A → R in AAB28444 (PubMed:8411151).Curated1
Sequence conflicti354S → C in AAA34941 (PubMed:8411151).Curated1
Sequence conflicti354S → C in AAD13975 (PubMed:8411151).Curated1
Sequence conflicti354S → C in AAB28444 (PubMed:8411151).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18779 Genomic DNA. Translation: AAB65002.1.
AY693014 Genomic DNA. Translation: AAT93033.1.
L22173 Genomic DNA. Translation: AAA34941.1. Frameshift.
S65964 Genomic DNA. Translation: AAD13975.1. Frameshift.
S66130 mRNA. Translation: AAB28444.1. Frameshift.
BK006939 Genomic DNA. Translation: DAA07613.1.
PIRiS30839.
RefSeqiNP_010874.3. NM_001178855.3.

Genome annotation databases

EnsemblFungiiYEL040W; YEL040W; YEL040W.
GeneIDi856671.
KEGGisce:YEL040W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18779 Genomic DNA. Translation: AAB65002.1.
AY693014 Genomic DNA. Translation: AAT93033.1.
L22173 Genomic DNA. Translation: AAA34941.1. Frameshift.
S65964 Genomic DNA. Translation: AAD13975.1. Frameshift.
S66130 mRNA. Translation: AAB28444.1. Frameshift.
BK006939 Genomic DNA. Translation: DAA07613.1.
PIRiS30839.
RefSeqiNP_010874.3. NM_001178855.3.

3D structure databases

ProteinModelPortaliP32623.
SMRiP32623.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36689. 77 interactors.
MINTiMINT-2785828.

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH16. Glycoside Hydrolase Family 16.

Proteomic databases

MaxQBiP32623.
PRIDEiP32623.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYEL040W; YEL040W; YEL040W.
GeneIDi856671.
KEGGisce:YEL040W.

Organism-specific databases

EuPathDBiFungiDB:YEL040W.
SGDiS000000766. UTR2.

Phylogenomic databases

GeneTreeiENSGT00610000086657.
HOGENOMiHOG000184016.
InParanoidiP32623.
OMAiLGGCDIR.
OrthoDBiEOG092C3MQ4.

Enzyme and pathway databases

BioCyciYEAST:G3O-30161-MONOMER.

Miscellaneous databases

PROiP32623.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. GH16.
IPR017168. Glyco_hydro_16_CRH1_prd.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PIRSFiPIRSF037299. Glycosidase_CRH1_prd. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51762. GH16_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRH2_YEAST
AccessioniPrimary (citable) accession number: P32623
Secondary accession number(s): D3DLK9, Q6B1R6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: April 18, 2006
Last modified: November 2, 2016
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.