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Protein

ATP-NADH kinase YEF1

Gene

YEF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-NADH kinase with a low phosphorylation activity of both NADH and NAD+ to produce NADP and NADPH by using ATP. UTR1 is responsible for essentially all of the NAD/NADH kinase activity resident in the cytoplasm, whereas POS5 is responsible for all mitochondrial NAD/NADH kinase activity and consequent mitochondrial genome maintenance. YEF1 can substitute for UTR1 when overexpressed.3 Publications

Catalytic activityi

ATP + NADH = ADP + NADPH.2 Publications

Cofactori

Mg2+1 Publication, Mn2+1 Publication, Co2+1 Publication, Ca2+1 Publication

Kineticsi

  1. KM=1.9 mM for NAD2 Publications
  2. KM=2.0 mM for NADH2 Publications
  3. KM=0.17 mM for ATP2 Publications
  1. Vmax=3.3 µmol/min/mg enzyme for NAD phosphorylation2 Publications
  2. Vmax=1.2 µmol/min/mg enzyme for NADH phosphorylation2 Publications

pH dependencei

Optimum pH is 8.5.2 Publications

Temperature dependencei

Optimum temperature is 45 degrees Celsius.2 Publications

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • NAD+ kinase activity Source: SGD
  • NADH kinase activity Source: SGD

GO - Biological processi

  • NAD metabolic process Source: InterPro
  • NADP biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Cobalt, Magnesium, Manganese, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:G3O-30162-MONOMER.
YEAST:G3O-30162-MONOMER.
BRENDAi2.7.1.23. 984.
2.7.1.86. 984.
ReactomeiR-SCE-196807. Nicotinate metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-NADH kinase YEF1 (EC:2.7.1.86)
Gene namesi
Name:YEF1
Ordered Locus Names:YEL041W
ORF Names:SYGP-ORF17
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL041W.
SGDiS000000767. YEF1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001207171 – 495ATP-NADH kinase YEF1Add BLAST495

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei475PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32622.
PRIDEiP32622.

PTM databases

iPTMnetiP32622.

Interactioni

Subunit structurei

Homooctamer.1 Publication

Protein-protein interaction databases

BioGridi36688. 19 interactors.
DIPiDIP-1885N.
IntActiP32622. 5 interactors.
MINTiMINT-402769.

Structurei

3D structure databases

ProteinModelPortaliP32622.
SMRiP32622.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000013792.
HOGENOMiHOG000141591.
InParanoidiP32622.
OMAiINAFENT.
OrthoDBiEOG092C2G28.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

P32622-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTDRLLINA SPETCTKGDA EMDTMDTIDR MTSVKVLAEG KVLSNFEEPG
60 70 80 90 100
LMRCGYHDAK NWVRRLSSET IVGEDTSNLY PFYVDTAYDV RRLRKDLINA
110 120 130 140 150
KVDLQVENLI IICNINDIST VFLMREVVEW ILRNFHSITV YVQDIFKKST
160 170 180 190 200
QFAVGDLCKD SNCSKNRVKY WSKEFVKKHD SFFDLMITLG GDGTVLFASS
210 220 230 240 250
IFTKDVPPIV PFALGSLGFL TNFEFQNFKE TLKHILTDEV RINLRMRLQC
260 270 280 290 300
KLYRRNKPEI DAATGRKICY IDFISEHHVL NEVTIDRGPA PCLSLLELYG
310 320 330 340 350
NDSLMTKVQG DGLIVATPTG STAYSLSAGG SLISPSVNAI AVTPICPHTL
360 370 380 390 400
SFRPIILPDS MELKVRVDMN SRGTSWVNFD GKDRVELKQG DYVVITASPY
410 420 430 440 450
SVPTIESSAS EFFESISKNL NWNDREEQKP FAHILSPKNQ EKYRLDSSKN
460 470 480 490
GNDTISNPLE SSCISSDAQD EERKSVTETE TEIVVERTRQ AHFAI
Length:495
Mass (Da):55,874
Last modified:October 1, 1993 - v1
Checksum:iE8301D466249DA27
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18779 Genomic DNA. Translation: AAB65001.1.
BK006939 Genomic DNA. Translation: DAA07612.1.
PIRiS30838.
RefSeqiNP_010873.1. NM_001178856.1.

Genome annotation databases

EnsemblFungiiYEL041W; YEL041W; YEL041W.
GeneIDi856670.
KEGGisce:YEL041W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18779 Genomic DNA. Translation: AAB65001.1.
BK006939 Genomic DNA. Translation: DAA07612.1.
PIRiS30838.
RefSeqiNP_010873.1. NM_001178856.1.

3D structure databases

ProteinModelPortaliP32622.
SMRiP32622.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36688. 19 interactors.
DIPiDIP-1885N.
IntActiP32622. 5 interactors.
MINTiMINT-402769.

PTM databases

iPTMnetiP32622.

Proteomic databases

MaxQBiP32622.
PRIDEiP32622.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYEL041W; YEL041W; YEL041W.
GeneIDi856670.
KEGGisce:YEL041W.

Organism-specific databases

EuPathDBiFungiDB:YEL041W.
SGDiS000000767. YEF1.

Phylogenomic databases

GeneTreeiENSGT00390000013792.
HOGENOMiHOG000141591.
InParanoidiP32622.
OMAiINAFENT.
OrthoDBiEOG092C2G28.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-30162-MONOMER.
YEAST:G3O-30162-MONOMER.
BRENDAi2.7.1.23. 984.
2.7.1.86. 984.
ReactomeiR-SCE-196807. Nicotinate metabolism.

Miscellaneous databases

PROiP32622.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiYEF1_YEAST
AccessioniPrimary (citable) accession number: P32622
Secondary accession number(s): D3DLK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.