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Protein

ATP-NADH kinase YEF1

Gene

YEF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-NADH kinase with a low phosphorylation activity of both NADH and NAD+ to produce NADP and NADPH by using ATP. UTR1 is responsible for essentially all of the NAD/NADH kinase activity resident in the cytoplasm, whereas POS5 is responsible for all mitochondrial NAD/NADH kinase activity and consequent mitochondrial genome maintenance. YEF1 can substitute for UTR1 when overexpressed.3 Publications

Miscellaneous

Present with 300 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + NADH = ADP + NADPH.2 Publications

Cofactori

Mg2+1 Publication, Mn2+1 Publication, Co2+1 Publication, Ca2+1 Publication

Kineticsi

  1. KM=1.9 mM for NAD2 Publications
  2. KM=2.0 mM for NADH2 Publications
  3. KM=0.17 mM for ATP2 Publications
  1. Vmax=3.3 µmol/min/mg enzyme for NAD phosphorylation2 Publications
  2. Vmax=1.2 µmol/min/mg enzyme for NADH phosphorylation2 Publications

pH dependencei

Optimum pH is 8.5.2 Publications

Temperature dependencei

Optimum temperature is 45 degrees Celsius.2 Publications

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • NAD+ kinase activity Source: SGD
  • NADH kinase activity Source: SGD

GO - Biological processi

  • NAD metabolic process Source: InterPro
  • NADP biosynthetic process Source: SGD

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Calcium, Cobalt, Magnesium, Manganese, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:G3O-30162-MONOMER
YEAST:G3O-30162-MONOMER
BRENDAi2.7.1.23 984
2.7.1.86 984

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-NADH kinase YEF1 (EC:2.7.1.86)
Gene namesi
Name:YEF1
Ordered Locus Names:YEL041W
ORF Names:SYGP-ORF17
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL041W
SGDiS000000767 YEF1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001207171 – 495ATP-NADH kinase YEF1Add BLAST495

Proteomic databases

MaxQBiP32622
PaxDbiP32622
PRIDEiP32622

PTM databases

iPTMnetiP32622

Interactioni

Subunit structurei

Homooctamer.1 Publication

Protein-protein interaction databases

BioGridi36688, 85 interactors
DIPiDIP-1885N
IntActiP32622, 7 interactors
MINTiP32622
STRINGi4932.YEL041W

Structurei

3D structure databases

ProteinModelPortaliP32622
SMRiP32622
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000013792
HOGENOMiHOG000141591
InParanoidiP32622
OMAiFKERTEF
OrthoDBiEOG092C2G28

Family and domain databases

Gene3Di2.60.200.30, 1 hit
HAMAPiMF_00361 NAD_kinase, 1 hit
InterProiView protein in InterPro
IPR017437 ATP-NAD_kinase_PpnK-typ_C
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002504 NADK
PANTHERiPTHR20275 PTHR20275, 1 hit
PfamiView protein in Pfam
PF01513 NAD_kinase, 1 hit
SUPFAMiSSF111331 SSF111331, 1 hit

Sequencei

Sequence statusi: Complete.

P32622-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTDRLLINA SPETCTKGDA EMDTMDTIDR MTSVKVLAEG KVLSNFEEPG
60 70 80 90 100
LMRCGYHDAK NWVRRLSSET IVGEDTSNLY PFYVDTAYDV RRLRKDLINA
110 120 130 140 150
KVDLQVENLI IICNINDIST VFLMREVVEW ILRNFHSITV YVQDIFKKST
160 170 180 190 200
QFAVGDLCKD SNCSKNRVKY WSKEFVKKHD SFFDLMITLG GDGTVLFASS
210 220 230 240 250
IFTKDVPPIV PFALGSLGFL TNFEFQNFKE TLKHILTDEV RINLRMRLQC
260 270 280 290 300
KLYRRNKPEI DAATGRKICY IDFISEHHVL NEVTIDRGPA PCLSLLELYG
310 320 330 340 350
NDSLMTKVQG DGLIVATPTG STAYSLSAGG SLISPSVNAI AVTPICPHTL
360 370 380 390 400
SFRPIILPDS MELKVRVDMN SRGTSWVNFD GKDRVELKQG DYVVITASPY
410 420 430 440 450
SVPTIESSAS EFFESISKNL NWNDREEQKP FAHILSPKNQ EKYRLDSSKN
460 470 480 490
GNDTISNPLE SSCISSDAQD EERKSVTETE TEIVVERTRQ AHFAI
Length:495
Mass (Da):55,874
Last modified:October 1, 1993 - v1
Checksum:iE8301D466249DA27
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18779 Genomic DNA Translation: AAB65001.1
BK006939 Genomic DNA Translation: DAA07612.1
PIRiS30838
RefSeqiNP_010873.1, NM_001178856.1

Genome annotation databases

EnsemblFungiiYEL041W; YEL041W; YEL041W
GeneIDi856670
KEGGisce:YEL041W

Similar proteinsi

Entry informationi

Entry nameiYEF1_YEAST
AccessioniPrimary (citable) accession number: P32622
Secondary accession number(s): D3DLK8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

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