P32622 (YEF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-NADH kinase YEF1
EC=2.7.1.86

Gene names

Name:	YEF1
Ordered Locus Names:	YEL041W
ORF Names:	SYGP-ORF17

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	ATP-NADH kinase with a low phosphorylation activity of both NADH and NAD+ to produce NADP and NADPH by using ATP. UTR1 is responsible for essentially all of the NAD/NADH kinase activity resident in the cytoplasm, whereas POS5 is responsible for all mitochondrial NAD/NADH kinase activity and consequent mitochondrial genome maintenance. YEF1 can substitute for UTR1 when overexpressed. Ref.4 Ref.5 Ref.6
Catalytic activity	ATP + NADH = ADP + NADPH. Ref.4 Ref.5
Cofactor	Magnesium, manganese, cobalt and calcium bivalent metal ions. Ref.4
Subunit structure	Homooctamer. Ref.4
Miscellaneous	Present with 300 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the NAD kinase family.
Biophysicochemical properties	Kinetic parameters: KM=1.9 mM for NAD Ref.4 Ref.5 KM=2.0 mM for NADH KM=0.17 mM for ATP Vmax=3.3 µmol/min/mg enzyme for NAD phosphorylation Vmax=1.2 µmol/min/mg enzyme for NADH phosphorylation pH dependence: Optimum pH is 8.5. Temperature dependence: Optimum temperature is 45 degrees Celsius.

Ontologies

Keywords
Ligand	ATP-binding Calcium Cobalt Magnesium Manganese NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NADP biosynthetic process Inferred from direct assay Ref.4. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from direct assay Ref.4. Source: SGD NADH kinase activity Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
UTR1	P21373	1	EBI-22350,EBI-20174

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 495	495	ATP-NADH kinase YEF1		PRO_0000120717

Sequences

	Sequence		Length	Mass (Da)
	P32622 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: E8301D466249DA27	FASTA	495	55,874
	10 20 30 40 50 60 MKTDRLLINA SPETCTKGDA EMDTMDTIDR MTSVKVLAEG KVLSNFEEPG LMRCGYHDAK 70 80 90 100 110 120 NWVRRLSSET IVGEDTSNLY PFYVDTAYDV RRLRKDLINA KVDLQVENLI IICNINDIST 130 140 150 160 170 180 VFLMREVVEW ILRNFHSITV YVQDIFKKST QFAVGDLCKD SNCSKNRVKY WSKEFVKKHD 190 200 210 220 230 240 SFFDLMITLG GDGTVLFASS IFTKDVPPIV PFALGSLGFL TNFEFQNFKE TLKHILTDEV 250 260 270 280 290 300 RINLRMRLQC KLYRRNKPEI DAATGRKICY IDFISEHHVL NEVTIDRGPA PCLSLLELYG 310 320 330 340 350 360 NDSLMTKVQG DGLIVATPTG STAYSLSAGG SLISPSVNAI AVTPICPHTL SFRPIILPDS 370 380 390 400 410 420 MELKVRVDMN SRGTSWVNFD GKDRVELKQG DYVVITASPY SVPTIESSAS EFFESISKNL 430 440 450 460 470 480 NWNDREEQKP FAHILSPKNQ EKYRLDSSKN GNDTISNPLE SSCISSDAQD EERKSVTETE 490 TEIVVERTRQ AHFAI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae." Shi F., Kawai S., Mori S., Kono E., Murata K. FEBS J. 272:3337-3349(2005) [PubMed: 15978040] [Abstract] Cited for: FUNCTION, SUBUNIT, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
[5]	"Synthetic lethal and biochemical analyses of NAD and NADH kinases in Saccharomyces cerevisiae establish separation of cellular functions." Bieganowski P., Seidle H.F., Wojcik M., Brenner C. J. Biol. Chem. 281:22439-22445(2006) [PubMed: 16760478] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
[6]	"Two sources of mitochondrial NADPH in the yeast Saccharomyces cerevisiae." Miyagi H., Kawai S., Murata K. J. Biol. Chem. 284:7553-7560(2009) [PubMed: 19158096] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18779 Genomic DNA. Translation: AAB65001.1. BK006939 Genomic DNA. Translation: DAA07612.1.
PIR	S30838.
RefSeq	NP_010873.1. NM_001178856.1.
3D structure databases
ProteinModelPortal	P32622.
SMR	P32622. Positions 105-422.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1885N.
IntAct	P32622. 3 interactions.
MINT	MINT-402769.
STRING	P32622.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL041W; YEL041W; YEL041W.
GeneID	856670.
KEGG	sce:YEL041W.
NMPDR	fig|4932.3.peg.1924.
Organism-specific databases
CYGD	YEL041w.
SGD	S000000767. YEF1.
Phylogenomic databases
eggNOG	fuNOG04457.
GeneTree	EFGT00050000004217.
HOGENOM	HBG713904.
OrthoDB	EOG4GQTDN.
Enzyme and pathway databases
BRENDA	2.7.1.23. 984.
Gene expression databases
ArrayExpress	P32622.
Genevestigator	P32622.
GermOnline	YEL041W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017438. ATP-NAD_kinase_PpnK-typ_a/b. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. polyP/ATP_NADK_prd. [Graphical view]
Gene3D	G3DSA:2.60.200.30. ATP-NAD_kinase_PpnK-typ. 1 hit. G3DSA:3.40.50.10330. ATP-NAD_kinase_PpnK-typ_a/b. 2 hits.
PANTHER	PTHR20275. ATP_NADK. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
SUPFAM	SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
ProtoNet	Search...
Other
NextBio	982681.

Entry information

Entry name

YEF1_YEAST

Accession

Primary (citable) accession number: P32622
Secondary accession number(s): D3DLK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program