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Protein

Guanosine-diphosphatase

Gene

GDA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

After transfer of sugars to endogenous macromolecular acceptors, the enzyme converts nucleoside diphosphates to nucleoside monophosphates which in turn exit the Golgi lumen in a coupled antiporter reaction, allowing entry of additional nucleotide sugar from the cytosol.1 Publication

Catalytic activityi

GDP + H2O = GMP + phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei216 – 2161Proton acceptorBy similarity

GO - Molecular functioni

  • guanosine-diphosphatase activity Source: SGD
  • uridine-diphosphatase activity Source: SGD

GO - Biological processi

  • protein glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciYEAST:YEL042W-MONOMER.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanosine-diphosphatase (EC:3.6.1.42)
Short name:
GDPase
Gene namesi
Name:GDA1
Ordered Locus Names:YEL042W
ORF Names:SYGP-ORF16
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL042W.
SGDiS000000768. GDA1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicSequence analysis
Transmembranei10 – 2415Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini25 – 518494LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi apparatus Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 518518Guanosine-diphosphatasePRO_0000209919Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi41 – 411N-linked (GlcNAc...)Sequence analysis
Glycosylationi280 – 2801N-linked (GlcNAc...)Sequence analysis
Glycosylationi335 – 3351N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP32621.

PTM databases

iPTMnetiP32621.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi36687. 136 interactions.
DIPiDIP-5284N.
IntActiP32621. 7 interactions.
MINTiMINT-475337.

Structurei

3D structure databases

ProteinModelPortaliP32621.
SMRiP32621. Positions 89-505.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GDA1/CD39 NTPase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000046675.
HOGENOMiHOG000206583.
InParanoidiP32621.
KOiK01526.
OMAiCAGESSW.
OrthoDBiEOG092C1F34.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 2 hits.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32621-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPIFRNYRF AIGAFAVIML ILLIKTSSIG PPSIARTVTP NASIPKTPED
60 70 80 90 100
ISILPVNDEP GYLQDSKTEQ NYPELADAVK SQTSQTCSEE HKYVIMIDAG
110 120 130 140 150
STGSRVHIYK FDVCTSPPTL LDEKFDMLEP GLSSFDTDSV GAANSLDPLL
160 170 180 190 200
KVAMNYVPIK ARSCTPVAVK ATAGLRLLGD AKSSKILSAV RDHLEKDYPF
210 220 230 240 250
PVVEGDGVSI MGGDEEGVFA WITTNYLLGN IGANGPKLPT AAVFDLGGGS
260 270 280 290 300
TQIVFEPTFP INEKMVDGEH KFDLKFGDEN YTLYQFSHLG YGLKEGRNKV
310 320 330 340 350
NSVLVENALK DGKILKGDNT KTHQLSSPCL PPKVNATNEK VTLESKETYT
360 370 380 390 400
IDFIGPDEPS GAQCRFLTDE ILNKDAQCQS PPCSFNGVHQ PSLVRTFKES
410 420 430 440 450
NDIYIFSYFY DRTRPLGMPL SFTLNELNDL ARIVCKGEET WNSVFSGIAG
460 470 480 490 500
SLDELESDSH FCLDLSFQVS LLHTGYDIPL QRELRTGKKI ANKEIGWCLG
510
ASLPLLKADN WKCKIQSA
Length:518
Mass (Da):56,822
Last modified:October 1, 1993 - v1
Checksum:i9A61182D5ED22ADF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19560 Genomic DNA. Translation: AAA34656.1.
U18779 Genomic DNA. Translation: AAB65000.1.
AY723798 Genomic DNA. Translation: AAU09715.1.
BK006939 Genomic DNA. Translation: DAA07611.1.
PIRiA40732.
RefSeqiNP_010872.1. NM_001178857.1.

Genome annotation databases

EnsemblFungiiYEL042W; YEL042W; YEL042W.
GeneIDi856669.
KEGGisce:YEL042W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19560 Genomic DNA. Translation: AAA34656.1.
U18779 Genomic DNA. Translation: AAB65000.1.
AY723798 Genomic DNA. Translation: AAU09715.1.
BK006939 Genomic DNA. Translation: DAA07611.1.
PIRiA40732.
RefSeqiNP_010872.1. NM_001178857.1.

3D structure databases

ProteinModelPortaliP32621.
SMRiP32621. Positions 89-505.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36687. 136 interactions.
DIPiDIP-5284N.
IntActiP32621. 7 interactions.
MINTiMINT-475337.

PTM databases

iPTMnetiP32621.

Proteomic databases

MaxQBiP32621.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYEL042W; YEL042W; YEL042W.
GeneIDi856669.
KEGGisce:YEL042W.

Organism-specific databases

EuPathDBiFungiDB:YEL042W.
SGDiS000000768. GDA1.

Phylogenomic databases

GeneTreeiENSGT00510000046675.
HOGENOMiHOG000206583.
InParanoidiP32621.
KOiK01526.
OMAiCAGESSW.
OrthoDBiEOG092C1F34.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciYEAST:YEL042W-MONOMER.

Miscellaneous databases

PROiP32621.

Family and domain databases

InterProiIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERiPTHR11782. PTHR11782. 2 hits.
PfamiPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEiPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGDA1_YEAST
AccessioniPrimary (citable) accession number: P32621
Secondary accession number(s): D3DLK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: September 7, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8680 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.