ID   YEF3_YEAST              Reviewed;         956 AA.
AC   P32618; D3DLK6; Q66RC2;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   27-MAR-2024, entry version 170.
DE   RecName: Full=Uncharacterized protein YEL043W;
GN   OrderedLocusNames=YEL043W; ORFNames=SYGP-ORF14;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169868;
RA   Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA   Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA   Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA   Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA   Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA   Botstein D., Davis R.W.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL   Nature 387:78-81(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   DOMAIN FIBRONECTIN TYPE-III.
RX   PubMed=8994808; DOI=10.1016/s0960-9822(02)70765-3;
RA   Bateman A., Chothia C.;
RT   "Fibronectin type III domains in yeast detected by a hidden Markov model.";
RL   Curr. Biol. 6:1544-1546(1996).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-154, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ADR376;
RX   PubMed=17330950; DOI=10.1021/pr060559j;
RA   Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA   Elias J.E., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of alpha-factor-arrested
RT   Saccharomyces cerevisiae.";
RL   J. Proteome Res. 6:1190-1197(2007).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-154, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA   Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA   Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT   "Analysis of phosphorylation sites on proteins from Saccharomyces
RT   cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-501; SER-520 AND SER-802, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-154; SER-520; SER-802;
RP   SER-842 AND SER-895, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP   ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- INTERACTION:
CC       P32618; P53253: NNF2; NbExp=3; IntAct=EBI-22354, EBI-23229;
CC   -!- MISCELLANEOUS: Present with 149 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; U18779; AAB64999.1; -; Genomic_DNA.
DR   EMBL; AY723799; AAU09716.1; -; Genomic_DNA.
DR   EMBL; BK006939; DAA07610.1; -; Genomic_DNA.
DR   PIR; S30834; S30834.
DR   RefSeq; NP_010871.3; NM_001178858.3.
DR   AlphaFoldDB; P32618; -.
DR   SMR; P32618; -.
DR   BioGRID; 36686; 232.
DR   DIP; DIP-2682N; -.
DR   IntAct; P32618; 8.
DR   MINT; P32618; -.
DR   STRING; 4932.YEL043W; -.
DR   GlyGen; P32618; 10 sites, 1 O-linked glycan (10 sites).
DR   iPTMnet; P32618; -.
DR   MaxQB; P32618; -.
DR   PaxDb; 4932-YEL043W; -.
DR   PeptideAtlas; P32618; -.
DR   EnsemblFungi; YEL043W_mRNA; YEL043W; YEL043W.
DR   GeneID; 856668; -.
DR   KEGG; sce:YEL043W; -.
DR   AGR; SGD:S000000769; -.
DR   SGD; S000000769; YEL043W.
DR   VEuPathDB; FungiDB:YEL043W; -.
DR   eggNOG; ENOG502R2RI; Eukaryota.
DR   HOGENOM; CLU_012632_0_0_1; -.
DR   InParanoid; P32618; -.
DR   OMA; NYSKGFT; -.
DR   OrthoDB; 2012158at2759; -.
DR   BioCyc; YEAST:G3O-30163-MONOMER; -.
DR   BioGRID-ORCS; 856668; 7 hits in 10 CRISPR screens.
DR   PRO; PR:P32618; -.
DR   Proteomes; UP000002311; Chromosome V.
DR   RNAct; P32618; Protein.
DR   GO; GO:0071944; C:cell periphery; HDA:SGD.
DR   GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR   CDD; cd00063; FN3; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR003961; FN3_dom.
DR   InterPro; IPR036116; FN3_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   SUPFAM; SSF49265; Fibronectin type III; 1.
DR   PROSITE; PS50853; FN3; 1.
PE   1: Evidence at protein level;
KW   Phosphoprotein; Reference proteome.
FT   CHAIN           1..956
FT                   /note="Uncharacterized protein YEL043W"
FT                   /id="PRO_0000202607"
FT   DOMAIN          40..141
FT                   /note="Fibronectin type-III"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT   REGION          152..173
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          488..600
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          875..956
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        488..544
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        551..600
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        890..956
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         154
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:17287358,
FT                   ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:19779198"
FT   MOD_RES         501
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956"
FT   MOD_RES         520
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956,
FT                   ECO:0007744|PubMed:19779198"
FT   MOD_RES         802
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956,
FT                   ECO:0007744|PubMed:19779198"
FT   MOD_RES         842
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19779198"
FT   MOD_RES         895
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19779198"
FT   CONFLICT        467
FT                   /note="F -> S (in Ref. 3; AAU09716)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   956 AA;  106133 MW;  3F78B09A0FCA03AF CRC64;
     MPVSVITTVL ACLWLSYRLY KFLTIPVSSI VSTLKIKTPP ATKVSIDKIA TDSVTIHWEN
     EPVKAEDNGS ADRNFISHYL LYLNNTQLAI FPNNPNSLYT CCSITGLEAE TQYQLDFITI
     NNKGFINKLP SIYCMTKARE ANEALKTRKW RRNTITSSTA MQPRNSKSEP APLPSHYSSV
     SLSTFSSNIT NSATSNNGSN LPAYTSLTTL KDLDSFSIDD LKKILICAQE DLHDVLSQQT
     SLLQDFQESK LELELELDNL KTHWSHEIDL RKSLKSNIKS LENSKLLTDL KIEKLNKKID
     KSKEKISKMR NDMQKWSQED TELLSKDTIK EKYFKLLNES NASVANINKE IESLQNEISK
     MEESNKRLNA SKKSLITSIV VNANVENDKP IASGELSAVL KKLNDFTLEK NGFLSNAGEE
     FLSKLNADSS LIKMIKQELS IDQELEANWK LQRSNLLKKI SALENQFNEM SLNNRNLKTK
     LMVQPYKNNG DSLAATNSNN SAEKNRSSGS IQLPLSNNMS RTGSIDLISN NNKSINNSNA
     DSAPPLRLHN PVSYSPSNEP IQPSSSLLSQ LTQDTDNRSM LSNHISSNNE NKQQPSSYSH
     ALPTTATANA TATATATNGH SRSNLWTTAQ FAQPSHQQVS TELDQAFEYD NANHLISGLQ
     NMIYDETDYP DNISNYSKGF TTDELDNYWT KQQPQVRSTN ESLFSTTGTP MSSYKANPVI
     SPYSSSHLRQ TSNATNTNPM HPQSLLAATL NDPSLQSFVR SGSFYSAPQP ANSLQNNING
     NETENISPRI SSDFNLLVPN LSPRLSNDVP IVPGNNTTLT PSHSNILTMN HQPTADNITR
     RSFHASSPPF NSIWNSNTNQ LSPPLEEQYH LDVPVGPKVP AKEPSPKPSH KRNQSNSSIS
     SAWSKFKHKS ASSPANADTD IQDSSTPSTS PSGRRMSKLL SKSGMNNLFN PHSHDS
//