Reviewed, UniProtKB/Swiss-Prot P32618 (YEF3_YEAST)

Names and origin

Protein names

Recommended name:
    Uncharacterized protein YEL043W

Gene names

Ordered Locus Names:	YEL043W
ORF Names:	SYGP-ORF14

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	956 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Miscellaneous	Present with 149 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Contains 1 fibronectin type-III domain.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	endoplasmic reticulum Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q12125	1	EBI-22354,EBI-36940

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 956	956	Uncharacterized protein YEL043W		PRO_0000202607
Regions
	Domain	35 – 125	91	Fibronectin type-III
Amino acid modifications
	Modified residue	154	1	Phosphothreonine Ref.5 Ref.6 Ref.8
	Modified residue	501	1	Phosphoserine Ref.8
	Modified residue	507	1	Phosphoserine Ref.8
	Modified residue	508	1	Phosphoserine Ref.8
	Modified residue	520	1	Phosphoserine Ref.8
	Modified residue	522	1	Phosphothreonine Ref.8
	Modified residue	524	1	Phosphoserine Ref.8
	Modified residue	792	1	Phosphoserine Ref.8
	Modified residue	802	1	Phosphoserine Ref.8
	Modified residue	930	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	467	1	F → S in AAU09716. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P32618-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 3F78B09A0FCA03AF	FASTA	956	106,133
	10 20 30 40 50 60 MPVSVITTVL ACLWLSYRLY KFLTIPVSSI VSTLKIKTPP ATKVSIDKIA TDSVTIHWEN 70 80 90 100 110 120 EPVKAEDNGS ADRNFISHYL LYLNNTQLAI FPNNPNSLYT CCSITGLEAE TQYQLDFITI 130 140 150 160 170 180 NNKGFINKLP SIYCMTKARE ANEALKTRKW RRNTITSSTA MQPRNSKSEP APLPSHYSSV 190 200 210 220 230 240 SLSTFSSNIT NSATSNNGSN LPAYTSLTTL KDLDSFSIDD LKKILICAQE DLHDVLSQQT 250 260 270 280 290 300 SLLQDFQESK LELELELDNL KTHWSHEIDL RKSLKSNIKS LENSKLLTDL KIEKLNKKID 310 320 330 340 350 360 KSKEKISKMR NDMQKWSQED TELLSKDTIK EKYFKLLNES NASVANINKE IESLQNEISK 370 380 390 400 410 420 MEESNKRLNA SKKSLITSIV VNANVENDKP IASGELSAVL KKLNDFTLEK NGFLSNAGEE 430 440 450 460 470 480 FLSKLNADSS LIKMIKQELS IDQELEANWK LQRSNLLKKI SALENQFNEM SLNNRNLKTK 490 500 510 520 530 540 LMVQPYKNNG DSLAATNSNN SAEKNRSSGS IQLPLSNNMS RTGSIDLISN NNKSINNSNA 550 560 570 580 590 600 DSAPPLRLHN PVSYSPSNEP IQPSSSLLSQ LTQDTDNRSM LSNHISSNNE NKQQPSSYSH 610 620 630 640 650 660 ALPTTATANA TATATATNGH SRSNLWTTAQ FAQPSHQQVS TELDQAFEYD NANHLISGLQ 670 680 690 700 710 720 NMIYDETDYP DNISNYSKGF TTDELDNYWT KQQPQVRSTN ESLFSTTGTP MSSYKANPVI 730 740 750 760 770 780 SPYSSSHLRQ TSNATNTNPM HPQSLLAATL NDPSLQSFVR SGSFYSAPQP ANSLQNNING 790 800 810 820 830 840 NETENISPRI SSDFNLLVPN LSPRLSNDVP IVPGNNTTLT PSHSNILTMN HQPTADNITR 850 860 870 880 890 900 RSFHASSPPF NSIWNSNTNQ LSPPLEEQYH LDVPVGPKVP AKEPSPKPSH KRNQSNSSIS 910 920 930 940 950 SAWSKFKHKS ASSPANADTD IQDSSTPSTS PSGRRMSKLL SKSGMNNLFN PHSHDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Fibronectin type III domains in yeast detected by a hidden Markov model." Bateman A., Chothia C. Curr. Biol. 6:1544-1546(1996) [PubMed: 8994808] [Abstract] Cited for: DOMAIN FIBRONECTIN TYPE-III.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-154, MASS SPECTROMETRY.
[6]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-154, MASS SPECTROMETRY.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-930, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-154; SER-501; SER-507; SER-508; SER-520; THR-522; SER-524; SER-792 AND SER-802, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18779 Genomic DNA. Translation: AAB64999.1. AY723799 Genomic DNA. Translation: AAU09716.1.
PIR	S30834.
RefSeq	NP_010871.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2682N.
IntAct	P32618. 10 interactions.
STRING	P32618.
Proteomic databases
PeptideAtlas	P32618.
Genome annotation databases
Ensembl	YEL043W; YEL043W; YEL043W; Saccharomyces cerevisiae. [Genome view]
GeneID	856668.
KEGG	sce:YEL043W.
NMPDR	fig|4932.3.peg.1922.
Organism-specific databases
CYGD	YEL043w.
SGD	S000000769. YEL043W.
Phylogenomic databases
HOGENOM	P32618.
OMA	TIHWENE
OrthoDB	EOG93JDMV
Gene expression databases
ArrayExpress	P32618.
Genevestigator	P32618.
GermOnline	YEL043W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003961. FN_III. [Graphical view]
SMART	SM00060. FN3. 1 hit. [Graphical view]
PROSITE	PS50853. FN3. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	982675.

Entry information

Entry name

YEF3_YEAST

Accession

Primary (citable) accession number: P32618
Secondary accession number(s): Q66RC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	November 24, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)