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P32618 (YEF3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Uncharacterized protein YEL043W
Gene names
Ordered Locus Names:YEL043W
ORF Names:SYGP-ORF14
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length956 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Miscellaneous

Present with 149 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Contains 1 fibronectin type-III domain.

Ontologies

Keywords
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componentendoplasmic reticulum

Inferred from direct assay. Source: SGD

   Molecular functionprotein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

NNF2P532533EBI-22354,EBI-23229

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 956956Uncharacterized protein YEL043W
PRO_0000202607

Regions

Domain35 – 12591Fibronectin type-III

Amino acid modifications

Modified residue1541Phosphothreonine Ref.6 Ref.7 Ref.9
Modified residue5011Phosphoserine Ref.9
Modified residue5071Phosphoserine Ref.9
Modified residue5081Phosphoserine Ref.9
Modified residue5201Phosphoserine Ref.9
Modified residue5221Phosphothreonine Ref.9
Modified residue5241Phosphoserine Ref.9
Modified residue7921Phosphoserine Ref.9
Modified residue8021Phosphoserine Ref.9
Modified residue9301Phosphoserine Ref.8

Experimental info

Sequence conflict4671F → S in AAU09716. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P32618 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 3F78B09A0FCA03AF

FASTA956106,133
        10         20         30         40         50         60 
MPVSVITTVL ACLWLSYRLY KFLTIPVSSI VSTLKIKTPP ATKVSIDKIA TDSVTIHWEN 

        70         80         90        100        110        120 
EPVKAEDNGS ADRNFISHYL LYLNNTQLAI FPNNPNSLYT CCSITGLEAE TQYQLDFITI 

       130        140        150        160        170        180 
NNKGFINKLP SIYCMTKARE ANEALKTRKW RRNTITSSTA MQPRNSKSEP APLPSHYSSV 

       190        200        210        220        230        240 
SLSTFSSNIT NSATSNNGSN LPAYTSLTTL KDLDSFSIDD LKKILICAQE DLHDVLSQQT 

       250        260        270        280        290        300 
SLLQDFQESK LELELELDNL KTHWSHEIDL RKSLKSNIKS LENSKLLTDL KIEKLNKKID 

       310        320        330        340        350        360 
KSKEKISKMR NDMQKWSQED TELLSKDTIK EKYFKLLNES NASVANINKE IESLQNEISK 

       370        380        390        400        410        420 
MEESNKRLNA SKKSLITSIV VNANVENDKP IASGELSAVL KKLNDFTLEK NGFLSNAGEE 

       430        440        450        460        470        480 
FLSKLNADSS LIKMIKQELS IDQELEANWK LQRSNLLKKI SALENQFNEM SLNNRNLKTK 

       490        500        510        520        530        540 
LMVQPYKNNG DSLAATNSNN SAEKNRSSGS IQLPLSNNMS RTGSIDLISN NNKSINNSNA 

       550        560        570        580        590        600 
DSAPPLRLHN PVSYSPSNEP IQPSSSLLSQ LTQDTDNRSM LSNHISSNNE NKQQPSSYSH 

       610        620        630        640        650        660 
ALPTTATANA TATATATNGH SRSNLWTTAQ FAQPSHQQVS TELDQAFEYD NANHLISGLQ 

       670        680        690        700        710        720 
NMIYDETDYP DNISNYSKGF TTDELDNYWT KQQPQVRSTN ESLFSTTGTP MSSYKANPVI 

       730        740        750        760        770        780 
SPYSSSHLRQ TSNATNTNPM HPQSLLAATL NDPSLQSFVR SGSFYSAPQP ANSLQNNING 

       790        800        810        820        830        840 
NETENISPRI SSDFNLLVPN LSPRLSNDVP IVPGNNTTLT PSHSNILTMN HQPTADNITR 

       850        860        870        880        890        900 
RSFHASSPPF NSIWNSNTNQ LSPPLEEQYH LDVPVGPKVP AKEPSPKPSH KRNQSNSSIS 

       910        920        930        940        950 
SAWSKFKHKS ASSPANADTD IQDSSTPSTS PSGRRMSKLL SKSGMNNLFN PHSHDS

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed: 9169868] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Fibronectin type III domains in yeast detected by a hidden Markov model."
Bateman A., Chothia C.
Curr. Biol. 6:1544-1546(1996) [PubMed: 8994808] [Abstract]
Cited for: DOMAIN FIBRONECTIN TYPE-III.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-154, MASS SPECTROMETRY.
Strain: ADR376.
[7]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-154, MASS SPECTROMETRY.
[8]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-930, MASS SPECTROMETRY.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-154; SER-501; SER-507; SER-508; SER-520; THR-522; SER-524; SER-792 AND SER-802, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U18779 Genomic DNA. Translation: AAB64999.1.
AY723799 Genomic DNA. Translation: AAU09716.1.
BK006939 Genomic DNA. Translation: DAA07610.1.
PIRS30834.
RefSeqNP_010871.1. NM_001178858.1.

3D structure databases

ProteinModelPortalP32618.
SMRP32618. Positions 451-481.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-2682N.
IntActP32618. 10 interactions.
MINTMINT-436832.
STRINGP32618.

Proteomic databases

PeptideAtlasP32618.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYEL043W; YEL043W; YEL043W.
GeneID856668.
KEGGsce:YEL043W.
NMPDRfig|4932.3.peg.1922.

Organism-specific databases

CYGDYEL043w.
SGDS000000769. YEL043W.

Phylogenomic databases

eggNOGfuNOG09848.
HOGENOMHBG203725.
OMATIHWENE.
OrthoDBEOG40GH0R.

Gene expression databases

ArrayExpressP32618.
GenevestigatorP32618.
GermOnlineYEL043W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR003961. Fibronectin_type3.
IPR013783. Ig-like_fold.
[Graphical view]
Gene3DG3DSA:2.60.40.10. Ig-like_fold. 1 hit.
SMARTSM00060. FN3. 1 hit.
[Graphical view]
PROSITEPS50853. FN3. False negative.
[Graphical view]
ProtoNetSearch...

Other

NextBio982675.

Entry information

Entry nameYEF3_YEAST
AccessionPrimary (citable) accession number: P32618
Secondary accession number(s): D3DLK6, Q66RC2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: December 14, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents