P32616 (YEF5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized protein YEL045C

Gene names

Ordered Locus Names:	YEL045C
ORF Names:	SYGP-ORF33

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	141 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required for optimal growth under alkaline conditions.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Induction	Repressed after alkaline pH stress. Ref.3
Disruption phenotype	Growth defect under alkaline conditions and citric acid stress. Ref.4 Ref.5 Ref.6

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 141	141	Uncharacterized protein YEL045C		PRO_0000202606
Regions
	Transmembrane	38 – 58	21	Helical; Potential
	Transmembrane	67 – 87	21	Helical; Potential
	Nucleotide binding	15 – 22	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P32616 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: F6604AC5343A5D5C	FASTA	141	16,469
	10 20 30 40 50 60 MKCHAKRTLA FLATALPLSG KSRACTRTPQ SFASGFRAAA PFLFSRCFAL CITHCALFHL 70 80 90 100 110 120 PYSFTGFSFY FFVFFRLFLH WIYAFCICNS TYVYIYKQVL SLPVKSSMCP SRLSCSVWYR 130 140 VILTYIYVHC FRWLVRNHLL S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The transcriptional response to alkaline pH in Saccharomyces cerevisiae: evidence for calcium-mediated signalling." Serrano R., Ruiz A., Bernal D., Chambers J.R., Arino J. Mol. Microbiol. 46:1319-1333(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[4]	"Copper and iron are the limiting factors for growth of the yeast Saccharomyces cerevisiae in an alkaline environment." Serrano R., Bernal D., Simon E., Arino J. J. Biol. Chem. 279:19698-19704(2004) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[5]	"Evidence of a new role for the high-osmolarity glycerol mitogen-activated protein kinase pathway in yeast: regulating adaptation to citric acid stress." Lawrence C.L., Botting C.H., Antrobus R., Coote P.J. Mol. Cell. Biol. 24:3307-3323(2004) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[6]	"A genomic screen for yeast vacuolar membrane ATPase mutants." Sambade M., Alba M., Smardon A.M., West R.W., Kane P.M. Genetics 170:1539-1551(2005) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18779 Genomic DNA. Translation: AAB64997.1. AY558443 Genomic DNA. Translation: AAS56769.1.
PIR	S30832.
3D structure databases
ProteinModelPortal	P32616.
ModBase	Search...
Protein-protein interaction databases
STRING	4932.YEL045C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL045C; YEL045C; YEL045C.
Organism-specific databases
CYGD	YEL045c.
SGD	S000000771. YEL045C.
Gene expression databases
Genevestigator	P32616.
GermOnline	YEL045C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

YEF5_YEAST

Accession

Primary (citable) accession number: P32616

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	April 3, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program