Reviewed, UniProtKB/Swiss-Prot P32616 (YEF5_YEAST)

Names and origin

Protein names

Recommended name:
    Uncharacterized protein YEL045C

Gene names

Ordered Locus Names:	YEL045C
ORF Names:	SYGP-ORF33

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	141 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Required for optimal growth under alkaline conditions. Ref.4 Ref.5 Ref.6
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Induction	Repressed after alkaline pH stress. Ref.3

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 141	141	Uncharacterized protein YEL045C		PRO_0000202606
Regions
	Transmembrane	38 – 58	21	Potential
	Transmembrane	67 – 87	21	Potential
	Nucleotide binding	15 – 22	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P32616-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: F6604AC5343A5D5C	FASTA	141	16,469
	10 20 30 40 50 60 MKCHAKRTLA FLATALPLSG KSRACTRTPQ SFASGFRAAA PFLFSRCFAL CITHCALFHL 70 80 90 100 110 120 PYSFTGFSFY FFVFFRLFLH WIYAFCICNS TYVYIYKQVL SLPVKSSMCP SRLSCSVWYR 130 140 VILTYIYVHC FRWLVRNHLL S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The transcriptional response to alkaline pH in Saccharomyces cerevisiae: evidence for calcium-mediated signalling." Serrano R., Ruiz A., Bernal D., Chambers J.R., Arino J. Mol. Microbiol. 46:1319-1333(2002) [PubMed: 12453218] [Abstract] Cited for: INDUCTION.
[4]	"Copper and iron are the limiting factors for growth of the yeast Saccharomyces cerevisiae in an alkaline environment." Serrano R., Bernal D., Simon E., Arino J. J. Biol. Chem. 279:19698-19704(2004) [PubMed: 14993228] [Abstract] Cited for: FUNCTION.
[5]	"Evidence of a new role for the high-osmolarity glycerol mitogen-activated protein kinase pathway in yeast: regulating adaptation to citric acid stress." Lawrence C.L., Botting C.H., Antrobus R., Coote P.J. Mol. Cell. Biol. 24:3307-3323(2004) [PubMed: 15060153] [Abstract] Cited for: FUNCTION.
[6]	"A genomic screen for yeast vacuolar membrane ATPase mutants." Sambade M., Alba M., Smardon A.M., West R.W., Kane P.M. Genetics 170:1539-1551(2005) [PubMed: 15937126] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18779 Genomic DNA. Translation: AAB64997.1. AY558443 Genomic DNA. Translation: AAS56769.1.
PIR	S30832.
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	YEL045C; YEL045C; YEL045C; Saccharomyces cerevisiae. [Genome view]
Organism-specific databases
CYGD	YEL045c.
SGD	S000000771. YEL045C.
Gene expression databases
ArrayExpress	P32616.
Genevestigator	P32616.
GermOnline	YEL045C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

YEF5_YEAST

Accession

Primary (citable) accession number: P32616

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	October 13, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)