Reviewed, UniProtKB/Swiss-Prot P32614 (FRDS_YEAST)

Names and origin

Protein names

Recommended name:
    Fumarate reductase
Alternative name(s):
    NADH
    EC=1.3.1.6
    NADH-dependent fumarate reductase
    FAD-dependent oxidoreductase FRDS

Gene names

Ordered Locus Names:	YEL047C
ORF Names:	SYGP-ORF35

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Could be a fumarate reductase.
Catalytic activity	Succinate + NAD+ = fumarate + NADH.
Cofactor	FAD.
Subcellular location	Mitochondrion. Cytoplasm.
Miscellaneous	Present with 7620 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: SGD mitochondrion Ref.4 Inferred from direct assay. Source: SGD plasma membrane enriched fraction Inferred from direct assay. Source: SGD
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro fumarate reductase (NADH) activity Ref.1 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct succinate dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P43582	1	EBI-22366,EBI-22766
ESS1	P22696	1	EBI-22366,EBI-6679
PRP40	P33203	1	EBI-22366,EBI-701
SET2	P46995	1	EBI-22366,EBI-16985

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Fumarate reductase		PRO_0000158669
Regions
	Nucleotide binding	6 – 20	15	AMP Potential
Sites
	Active site	249	1	By similarity
	Active site	272	1	By similarity
Amino acid modifications
	Modified residue	66	1	Phosphoserine Ref.5
Experimental info
	Sequence conflict	285	1	R → K AA sequence Ref.1
	Sequence conflict	440	1	V → L AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32614-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 63C30B39252DC935	FASTA	470	50,844
	10 20 30 40 50 60 MSLSPVVVIG TGLAGLAAAN ELVNKYNIPV TILEKASSIG GNSIKASSGI NGACTETQRH 70 80 90 100 110 120 FHIEDSPRLF EDDTIKSAKG KGVQELMAKL ANDSPLAIEW LKNEFDLKLD LLAQLGGHSV 130 140 150 160 170 180 ARTHRSSGKL PPGFEIVSAL SNNLKKLAET KPELVKINLD SKVVDIHEKD GSISAVVYED 190 200 210 220 230 240 KNGEKHMVSA NDVVFCSGGF GFSKEMLKEY APELVNLPTT NGQQTTGDGQ RLLQKLGADL 250 260 270 280 290 300 IDMDQIQVHP TGFIDPNDRS SSWKFLAAES LRGLGGILLN PITGRRFVNE LTTRDVVTAA 310 320 330 340 350 360 IQKVCPQEDN RALLVMGEKM YTDLKNNLDF YMFKKLVQKL TLSQVVSEYN LPITVAQLCE 370 380 390 400 410 420 ELQTYSSFTT KADPLGRTVI LNEFGSDVTP ETVVFIGEVT PVVHFTMGGA RINVKAQVIG 430 440 450 460 470 KNDERLLKGL YAAGEVSGGV HGANRLGGSS LLECVVFGRT AAESIANDRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the gene encoding the soluble fumarate reductase from Saccharomyces cerevisiae." Enomoto K., Ohki R., Muratsubaki H. DNA Res. 3:263-267(1996) [PubMed: 8946166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 89-106; 245-253; 322-327 AND 409-423, SUBCELLULAR LOCATION.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18779 Genomic DNA. Translation: AAB64995.1.
PIR	S30830.
RefSeq	NP_010867.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5287N.
IntAct	P32614. 5 interactions.
STRING	P32614.
Proteomic databases
PeptideAtlas	P32614.
PRIDE	P32614.
Genome annotation databases
Ensembl	YEL047C; YEL047C; YEL047C; Saccharomyces cerevisiae. [Genome view]
GeneID	856664.
KEGG	sce:YEL047C.
NMPDR	fig|4932.3.peg.1918.
Organism-specific databases
CYGD	YEL047c.
SGD	S000000773. YEL047C.
Phylogenomic databases
HOGENOM	P32614.
OMA	YRENAAK
OrthoDB	EOG9S7M67
Enzyme and pathway databases
BRENDA	1.3.1.6. 250.
Gene expression databases
ArrayExpress	P32614.
Genevestigator	P32614.
GermOnline	YEL047C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR010960. Flavocytochrome_c. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01813. flavo_cyto_c. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	982666.

Entry information

Entry name

FRDS_YEAST

Accession

Primary (citable) accession number: P32614

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	November 24, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)