P32614 (FRDS_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fumarate reductase

Alternative name(s):
FAD-dependent oxidoreductase FRDS
NADH
EC=1.3.1.6
NADH-dependent fumarate reductase

Gene names

Ordered Locus Names:	YEL047C
ORF Names:	SYGP-ORF35

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Could be a fumarate reductase.
Catalytic activity	Succinate + NAD+ = fumarate + NADH.
Cofactor	FAD.
Subcellular location	Mitochondrion. Cytoplasm Ref.1 Ref.5.
Miscellaneous	Present with 7620 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cellular response to anoxia Inferred from genetic interaction. Source: SGD
Cellular component	cytosol Inferred from direct assay. Source: SGD mitochondrion Inferred from direct assay Ref.5. Source: SGD plasma membrane enriched fraction Inferred from direct assay. Source: SGD
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro fumarate reductase (NADH) activity Inferred from direct assay Ref.1. Source: SGD succinate dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Fumarate reductase		PRO_0000158669
Regions
	Nucleotide binding	6 – 20	15	AMP Potential
Sites
	Active site	249	1	By similarity
	Active site	272	1	By similarity
Amino acid modifications
	Modified residue	66	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	285	1	R → K AA sequence Ref.1
	Sequence conflict	440	1	V → L AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32614 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 63C30B39252DC935	FASTA	470	50,844
	10 20 30 40 50 60 MSLSPVVVIG TGLAGLAAAN ELVNKYNIPV TILEKASSIG GNSIKASSGI NGACTETQRH 70 80 90 100 110 120 FHIEDSPRLF EDDTIKSAKG KGVQELMAKL ANDSPLAIEW LKNEFDLKLD LLAQLGGHSV 130 140 150 160 170 180 ARTHRSSGKL PPGFEIVSAL SNNLKKLAET KPELVKINLD SKVVDIHEKD GSISAVVYED 190 200 210 220 230 240 KNGEKHMVSA NDVVFCSGGF GFSKEMLKEY APELVNLPTT NGQQTTGDGQ RLLQKLGADL 250 260 270 280 290 300 IDMDQIQVHP TGFIDPNDRS SSWKFLAAES LRGLGGILLN PITGRRFVNE LTTRDVVTAA 310 320 330 340 350 360 IQKVCPQEDN RALLVMGEKM YTDLKNNLDF YMFKKLVQKL TLSQVVSEYN LPITVAQLCE 370 380 390 400 410 420 ELQTYSSFTT KADPLGRTVI LNEFGSDVTP ETVVFIGEVT PVVHFTMGGA RINVKAQVIG 430 440 450 460 470 KNDERLLKGL YAAGEVSGGV HGANRLGGSS LLECVVFGRT AAESIANDRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the gene encoding the soluble fumarate reductase from Saccharomyces cerevisiae." Enomoto K., Ohki R., Muratsubaki H. DNA Res. 3:263-267(1996) [PubMed: 8946166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 89-106; 245-253; 322-327 AND 409-423, SUBCELLULAR LOCATION.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66, MASS SPECTROMETRY. Strain: ADR376.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18779 Genomic DNA. Translation: AAB64995.1. BK006939 Genomic DNA. Translation: DAA07607.1.
PIR	S30830.
RefSeq	NP_010867.1. NM_001178862.1.
3D structure databases
ProteinModelPortal	P32614.
SMR	P32614. Positions 3-469.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5287N.
IntAct	P32614. 4 interactions.
MINT	MINT-534771.
STRING	P32614.
Proteomic databases
PeptideAtlas	P32614.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL047C; YEL047C; YEL047C.
GeneID	856664.
KEGG	sce:YEL047C.
NMPDR	fig|4932.3.peg.1918.
Organism-specific databases
CYGD	YEL047c.
SGD	S000000773. YEL047C.
Phylogenomic databases
eggNOG	fuNOG04455.
GeneTree	EFGT00050000001028.
HOGENOM	HBG678063.
OMA	CADCHGK.
OrthoDB	EOG43V340.
Gene expression databases
ArrayExpress	P32614.
Genevestigator	P32614.
GermOnline	YEL047C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR010960. Flavocytochrome_c. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01813. Flavo_cyto_c. 1 hit.
ProtoNet	Search...
Other
NextBio	982666.

Entry information

Entry name

FRDS_YEAST

Accession

Primary (citable) accession number: P32614
Secondary accession number(s): D3DLK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program