P32613 (TCA17_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
TRAPP-associated protein TCA17

Alternative name(s):
17 kDa TRAPP complex-associated protein

Gene names

Name:	TCA17
Ordered Locus Names:	YEL048C
ORF Names:	SYGP-ORF36

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required, together with the TRAPP II subunit TRS33, for TRAPP II complex assembly or stability, and for proper Golgi localization of TRAPP and the Rab GTPase YPT31. Ref.5 Ref.6
Subunit structure	Interacts with the TRAPP II complex; TRAPP II subunits TRS33 and TRS65 are required for this interaction. Ref.5 Ref.6
Subcellular location	Golgi apparatus › trans-Golgi network Ref.5.
Miscellaneous	Present with 8660 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the TRAPP small subunits family. Sedlin subfamily.

Ontologies

Keywords
Cellular component	Golgi apparatus
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from electronic annotation. Source: InterPro early endosome to Golgi transport Inferred from mutant phenotype Ref.5. Source: SGD protein complex assembly Inferred from mutant phenotype. Source: SGD
Cellular component	TRAPP complex Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	TRAPP-associated protein TCA17		PRO_0000202605
Experimental info
	Sequence conflict	2	1	S → T in AAS56768. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P32613 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: F7AF8E6EC34F193D	FASTA	152	17,354
	10 20 30 40 50 60 MSLRPCFVSL IDESDKPILI YVPNEAENEM NDVLKYNVLS NISLDYFESA LVEWHSLDSK 70 80 90 100 110 120 PLLKSIFQLE GVSVFAMLIK QTGLKIVIGF EQKSLSGADD EFEAINQIFE TVRKIYIRVK 130 140 150 CNPLLVSGDE KSIIKSLERK FDELFISTEV EL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Identification of the novel TRAPP associated protein Tca17." Montpetit B., Conibear E. Traffic 10:713-723(2009) [PubMed: 19220810] [Abstract] Cited for: FUNCTION, INTERACTION WITH THE TRAPP II COMPLEX, SUBCELLULAR LOCATION.
[6]	"TRAPPC2L is a novel, highly conserved TRAPP-interacting protein." Scrivens P.J., Shahrzad N., Moores A., Morin A., Brunet S., Sacher M. Traffic 10:724-736(2009) [PubMed: 19416478] [Abstract] Cited for: FUNCTION, INTERACTION WITH THE TRAPP II COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U18779 Genomic DNA. Translation: AAB64994.1.
AY558442 Genomic DNA. Translation: AAS56768.1.
BK006939 Genomic DNA. Translation: DAA07606.1.

PIR

S30829.

RefSeq

NP_010866.1. NM_001178863.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3PR6	X-ray	1.80	A	1-152	[»]

ProteinModelPortal

P32613.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5288N.

IntAct

P32613. 3 interactions.

MINT

MINT-491635.

STRING

P32613.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YEL048C; YEL048C; YEL048C.

GeneID

856663.

KEGG

sce:YEL048C.

NMPDR

fig|4932.3.peg.1917.

Organism-specific databases

CYGD

YEL048c.

SGD

S000000774. TCA17.

Phylogenomic databases

eggNOG

fuNOG12944.

HOGENOM

HBG203723.

OMA

NISLDYF.

OrthoDB

EOG4PCF2W.

Gene expression databases

ArrayExpress

P32613.

Genevestigator

P32613.

GermOnline

YEL048C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR011012. Longin-like.
IPR006722. Sedlin.
[Graphical view]

Pfam

PF04628. Sedlin_N. 1 hit.
[Graphical view]

SUPFAM

SSF64356. Longin_like. 1 hit.

ProtoNet

Search...

Other

NextBio

982663.

Entry information

Entry name

TCA17_YEAST

Accession

Primary (citable) accession number: P32613
Secondary accession number(s): D3DLK2, Q6Q561

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program