Reviewed, UniProtKB/Swiss-Prot P32613 (TCA17_YEAST)

Names and origin

Protein names

Recommended name:
    TRAPP-associated protein TCA17
Alternative name(s):
    17 kDa TRAPP complex-associated protein

Gene names

Name:	TCA17
Ordered Locus Names:	YEL048C
ORF Names:	SYGP-ORF36

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required, together with the TRAPP II subunit TRS33, for TRAPP II complex assembly or stability, and for proper Golgi localization of TRAPP and the Rab GTPase YPT31. Ref.4 Ref.5
Subunit structure	Interacts with the TRAPP II complex; TRAPP II subunits TRS33 and TRS65 are required for this interaction. Ref.4 Ref.5
Subcellular location	Golgi apparatus › trans-Golgi network.
Miscellaneous	Present with 8660 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the TRAPP small subunits family. Sedlin subfamily.

Ontologies

Keywords
Cellular component	Golgi apparatus
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ER to Golgi vesicle-mediated transport Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Ref.4 Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
KSS1	P14681	1	EBI-22370,EBI-9945

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	TRAPP-associated protein TCA17		PRO_0000202605
Experimental info
	Sequence conflict	2	1	S → T in AAS56768. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P32613-1 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: F7AF8E6EC34F193D	FASTA	152	17,354
	10 20 30 40 50 60 MSLRPCFVSL IDESDKPILI YVPNEAENEM NDVLKYNVLS NISLDYFESA LVEWHSLDSK 70 80 90 100 110 120 PLLKSIFQLE GVSVFAMLIK QTGLKIVIGF EQKSLSGADD EFEAINQIFE TVRKIYIRVK 130 140 150 CNPLLVSGDE KSIIKSLERK FDELFISTEV EL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"Identification of the novel TRAPP associated protein Tca17." Montpetit B., Conibear E. Traffic 10:713-723(2009) [PubMed: 19220810] [Abstract] Cited for: FUNCTION, INTERACTION WITH THE TRAPP II COMPLEX, SUBCELLULAR LOCATION.
[5]	"TRAPPC2L is a novel, highly conserved TRAPP-interacting protein." Scrivens P.J., Shahrzad N., Moores A., Morin A., Brunet S., Sacher M. Traffic 10:724-736(2009) [PubMed: 19416478] [Abstract] Cited for: FUNCTION, INTERACTION WITH THE TRAPP II COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18779 Genomic DNA. Translation: AAB64994.1. AY558442 Genomic DNA. Translation: AAS56768.1.
PIR	S30829.
RefSeq	NP_010866.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5288N.
IntAct	P32613. 7 interactions.
STRING	P32613.
Genome annotation databases
Ensembl	YEL048C; YEL048C; YEL048C; Saccharomyces cerevisiae. [Genome view]
GeneID	856663.
KEGG	sce:YEL048C.
NMPDR	fig|4932.3.peg.1917.
Organism-specific databases
CYGD	YEL048c.
SGD	S000000774. TCA17.
Phylogenomic databases
HOGENOM	P32613.
OMA	NISLDYF
OrthoDB	EOG90K9FX
Gene expression databases
ArrayExpress	P32613.
Genevestigator	P32613.
GermOnline	YEL048C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011012. Longin-like. IPR006722. Sedlin. [Graphical view]
Pfam	PF04628. Sedlin_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	982663.

Entry information

Entry name

TCA17_YEAST

Accession

Primary (citable) accession number: P32613
Secondary accession number(s): Q6Q561

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	November 24, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)