P32611 (RML2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
54S ribosomal protein RML2, mitochondrial
Short name=L2

Gene names

Name:	RML2
Ordered Locus Names:	YEL050C
ORF Names:	SYGP-ORF37

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.3
Subcellular location	Mitochondrion.
Miscellaneous	Present with 1600 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the ribosomal protein L2P family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cytoplasmic translation Traceable author statement. Source: SGD fatty acid metabolic process Inferred from mutant phenotype Ref.4. Source: SGD
Cellular component	mitochondrial large ribosomal subunit Traceable author statement. Source: SGD
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro structural constituent of ribosome Traceable author statement. Source: SGD transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 43	43	Mitochondrion Potential
	Chain	44 – 393	350	54S ribosomal protein RML2, mitochondrial		PRO_0000030512
Experimental info
	Mutagenesis	336 – 342	7	Missing: Loss of function. Ref.3
	Mutagenesis	343	1	H → Q: Causes a cold-sensitive respiratory growth defect. Does not impair assembly of the ribosomal subunit. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P32611 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 6AD955FF61C24923	FASTA	393	43,786
	10 20 30 40 50 60 MLVLGSLRSA LSCSSTASLI SKRNPCYPYG ILCRTLSQSV KLWQENTSKD DSSLNITPRL 70 80 90 100 110 120 LKIIPNDTDI VTLEKQDELI KRRRKLSKEV TQMKRLKPVS PGLRWYRSPI YPYLYKGRPV 130 140 150 160 170 180 RALTVVRKKH GGRNNSGKIT VRHQGGGHRN RTRLIDFNRW EGGAQTVQRI EYDPGRSSHI 190 200 210 220 230 240 ALLKHNTTGE LSYIIACDGL RPGDVVESFR RGIPQTLLNE MGGKVDPAIL SVKTTQRGNC 250 260 270 280 290 300 LPISMIPIGT IIHNVGITPV GPGKFCRSAG TYARVLAKLP EKKKAIVRLQ SGEHRYVSLE 310 320 330 340 350 360 AVATIGVVSN IDHQNRSLGK AGRSRWLGIR PTVRGVAMNK CDHPHGGGRG KSKSNKLSMS 370 380 390 PWGQLAKGYK TRRGKNQNRM KVKDRPRGKD ARL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Functional analysis of ribosomal protein L2 in yeast mitochondria." Pan C., Mason T.L. J. Biol. Chem. 272:8165-8171(1997) [PubMed: 9079633] [Abstract] Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF 336-VAL--ASP-342 AND HIS-343.
[4]	"A yeast strain defective in oleic acid utilization has a mutation in the RML2 gene." Trotter P.J., Hagerman R.A., Voelker D.R. Biochim. Biophys. Acta 1438:223-238(1999) [PubMed: 10320805] [Abstract] Cited for: FUNCTION.
[5]	"A mutation in the yeast mitochondrial ribosomal protein Rml2p is associated with a defect in catalase gene expression." Hagerman R.A., Trotter P.J. Mol. Cell Biol. Res. Commun. 4:299-306(2001) [PubMed: 11529680] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18779 Genomic DNA. Translation: AAB64992.1. BK006939 Genomic DNA. Translation: DAA07604.1.
PIR	S30827.
RefSeq	NP_010864.1. NM_001178865.1.
3D structure databases
ProteinModelPortal	P32611.
SMR	P32611. Positions 92-387.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6812N.
IntAct	P32611. 2 interactions.
MINT	MINT-615808.
STRING	P32611.
Proteomic databases
PeptideAtlas	P32611.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL050C; YEL050C; YEL050C.
GeneID	856660.
KEGG	sce:YEL050C.
NMPDR	fig|4932.3.peg.1915.
Organism-specific databases
CYGD	YEL050c.
SGD	S000000776. RML2.
Phylogenomic databases
eggNOG	fuNOG04946.
GeneTree	EFGT00050000005193.
HOGENOM	HBG748739.
OMA	QRGNCLP.
OrthoDB	EOG4W9NCQ.
Gene expression databases
ArrayExpress	P32611.
Genevestigator	P32611.
GermOnline	YEL050C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR022666. Rbsml_prot_L2_RNA-bd_dom. IPR002171. Ribosomal_L2. IPR014726. Ribosomal_L2_3. IPR005880. Ribosomal_L2_bac-type. IPR022669. Ribosomal_L2_C. IPR022671. Ribosomal_L2_CS. IPR014722. Transl_SH3-like_sub. IPR008991. Translation_prot_SH3-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:2.30.30.30. Ribosomal_L2. 1 hit. G3DSA:4.10.950.10. Ribosomal_L2. 1 hit.
PANTHER	PTHR13691:SF5. Ribosom_L2_bac. 1 hit. PTHR13691. Ribosomal_L2. 1 hit.
Pfam	PF00181. Ribosomal_L2. 1 hit. PF03947. Ribosomal_L2_C. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF50104. Transl_SH3_like. 1 hit.
TIGRFAMs	TIGR01171. RplB_bact. 1 hit.
PROSITE	PS00467. RIBOSOMAL_L2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982655.

Entry information

Entry name

RML2_YEAST

Accession

Primary (citable) accession number: P32611
Secondary accession number(s): D3DLK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 14, 2011
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program