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P32611 (RML2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
54S ribosomal protein RML2, mitochondrial

Short name=L2
Gene names
Name:RML2
Ordered Locus Names:YEL050C
ORF Names:SYGP-ORF37
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.3

Subcellular location

Mitochondrion.

Miscellaneous

Present with 1600 molecules/cell in log phase SD medium. Ref.6

Sequence similarities

Belongs to the ribosomal protein L2P family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4343Mitochondrion Potential
Chain44 – 39335054S ribosomal protein RML2, mitochondrial
PRO_0000030512

Experimental info

Mutagenesis336 – 3427Missing: Loss of function. Ref.3
Mutagenesis3431H → Q: Causes a cold-sensitive respiratory growth defect. Does not impair assembly of the ribosomal subunit. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P32611 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 6AD955FF61C24923

FASTA39343,786
        10         20         30         40         50         60 
MLVLGSLRSA LSCSSTASLI SKRNPCYPYG ILCRTLSQSV KLWQENTSKD DSSLNITPRL 

        70         80         90        100        110        120 
LKIIPNDTDI VTLEKQDELI KRRRKLSKEV TQMKRLKPVS PGLRWYRSPI YPYLYKGRPV 

       130        140        150        160        170        180 
RALTVVRKKH GGRNNSGKIT VRHQGGGHRN RTRLIDFNRW EGGAQTVQRI EYDPGRSSHI 

       190        200        210        220        230        240 
ALLKHNTTGE LSYIIACDGL RPGDVVESFR RGIPQTLLNE MGGKVDPAIL SVKTTQRGNC 

       250        260        270        280        290        300 
LPISMIPIGT IIHNVGITPV GPGKFCRSAG TYARVLAKLP EKKKAIVRLQ SGEHRYVSLE 

       310        320        330        340        350        360 
AVATIGVVSN IDHQNRSLGK AGRSRWLGIR PTVRGVAMNK CDHPHGGGRG KSKSNKLSMS 

       370        380        390 
PWGQLAKGYK TRRGKNQNRM KVKDRPRGKD ARL 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed: 9169868] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Functional analysis of ribosomal protein L2 in yeast mitochondria."
Pan C., Mason T.L.
J. Biol. Chem. 272:8165-8171(1997) [PubMed: 9079633] [Abstract]
Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF 336-VAL--ASP-342 AND HIS-343.
[4]"A yeast strain defective in oleic acid utilization has a mutation in the RML2 gene."
Trotter P.J., Hagerman R.A., Voelker D.R.
Biochim. Biophys. Acta 1438:223-238(1999) [PubMed: 10320805] [Abstract]
Cited for: FUNCTION.
[5]"A mutation in the yeast mitochondrial ribosomal protein Rml2p is associated with a defect in catalase gene expression."
Hagerman R.A., Trotter P.J.
Mol. Cell Biol. Res. Commun. 4:299-306(2001) [PubMed: 11529680] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18779 Genomic DNA. Translation: AAB64992.1.
BK006939 Genomic DNA. Translation: DAA07604.1.
PIRS30827.
RefSeqNP_010864.1. NM_001178865.1.

3D structure databases

ProteinModelPortalP32611.
SMRP32611. Positions 92-387.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-6812N.
IntActP32611. 2 interactions.
MINTMINT-615808.
STRINGP32611.

Proteomic databases

PeptideAtlasP32611.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYEL050C; YEL050C; YEL050C.
GeneID856660.
KEGGsce:YEL050C.
NMPDRfig|4932.3.peg.1915.

Organism-specific databases

CYGDYEL050c.
SGDS000000776. RML2.

Phylogenomic databases

eggNOGfuNOG04946.
GeneTreeEFGT00050000005193.
HOGENOMHBG748739.
OMAQRGNCLP.
OrthoDBEOG4W9NCQ.

Gene expression databases

ArrayExpressP32611.
GenevestigatorP32611.
GermOnlineYEL050C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR022666. Rbsml_prot_L2_RNA-bd_dom.
IPR002171. Ribosomal_L2.
IPR014726. Ribosomal_L2_3.
IPR005880. Ribosomal_L2_bac-type.
IPR022669. Ribosomal_L2_C.
IPR022671. Ribosomal_L2_CS.
IPR014722. Transl_SH3-like_sub.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:2.30.30.30. Ribosomal_L2. 1 hit.
G3DSA:4.10.950.10. Ribosomal_L2. 1 hit.
PANTHERPTHR13691:SF5. Ribosom_L2_bac. 1 hit.
PTHR13691. Ribosomal_L2. 1 hit.
PfamPF00181. Ribosomal_L2. 1 hit.
PF03947. Ribosomal_L2_C. 1 hit.
[Graphical view]
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF50104. Transl_SH3_like. 1 hit.
TIGRFAMsTIGR01171. RplB_bact. 1 hit.
PROSITEPS00467. RIBOSOMAL_L2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio982655.

Entry information

Entry nameRML2_YEAST
AccessionPrimary (citable) accession number: P32611
Secondary accession number(s): D3DLK0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: December 14, 2011
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Ribosomal proteins

Ribosomal proteins families and list of entries

SIMILARITY comments

Index of protein domains and families