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Protein

54S ribosomal protein RML2, mitochondrial

Gene

RML2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.2 Publications

Miscellaneous

Present with 1600 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • RNA binding Source: GO_Central
  • structural constituent of ribosome Source: SGD
  • transferase activity Source: InterPro

GO - Biological processi

  • mitochondrial translation Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-30168-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
54S ribosomal protein RML2, mitochondrial
Short name:
L2
Alternative name(s):
Mitochondrial large ribosomal subunit protein uL2m1 Publication
Gene namesi
Name:RML2
Ordered Locus Names:YEL050C
ORF Names:SYGP-ORF37
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL050C
SGDiS000000776 RML2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi336 – 342Missing : Loss of function. 1 Publication7
Mutagenesisi343H → Q: Causes a cold-sensitive respiratory growth defect. Does not impair assembly of the ribosomal subunit. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 43MitochondrionSequence analysisAdd BLAST43
ChainiPRO_000003051244 – 39354S ribosomal protein RML2, mitochondrialAdd BLAST350

Proteomic databases

MaxQBiP32611
PaxDbiP32611
PRIDEiP32611

Interactioni

Subunit structurei

Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature yeast 74S mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S small subunit contains a 15S ribosomal RNA (15S mt-rRNA) and 34 different proteins. The 54S large subunit contains a 21S rRNA (21S mt-rRNA) and 46 different proteins. uL2m has a Na/K ligand binding site.2 Publications

Protein-protein interaction databases

BioGridi36679, 489 interactors
DIPiDIP-6812N
IntActiP32611, 4 interactors
MINTiP32611
STRINGi4932.YEL050C

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Belectron microscopy3.20B1-393[»]
5MRCelectron microscopy3.25B1-393[»]
5MREelectron microscopy3.75B1-393[»]
5MRFelectron microscopy4.97B1-393[»]
ProteinModelPortaliP32611
SMRiP32611
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000000189
HOGENOMiHOG000232982
InParanoidiP32611
OMAiDACATIG
OrthoDBiEOG092C2Z0X

Family and domain databases

Gene3Di2.30.30.30, 1 hit
4.10.950.10, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR022666 Rbsml_prot_L2_RNA-bd_dom
IPR014722 Rib_L2_dom2
IPR002171 Ribosomal_L2
IPR005880 Ribosomal_L2_bac/org-type
IPR022669 Ribosomal_L2_C
IPR022671 Ribosomal_L2_CS
IPR014726 Ribosomal_L2_dom3
IPR008991 Translation_prot_SH3-like_sf
PANTHERiPTHR13691 PTHR13691, 1 hit
PfamiView protein in Pfam
PF00181 Ribosomal_L2, 1 hit
PF03947 Ribosomal_L2_C, 1 hit
SMARTiView protein in SMART
SM01383 Ribosomal_L2, 1 hit
SM01382 Ribosomal_L2_C, 1 hit
SUPFAMiSSF50104 SSF50104, 1 hit
SSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR01171 rplB_bact, 1 hit
PROSITEiView protein in PROSITE
PS00467 RIBOSOMAL_L2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32611-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVLGSLRSA LSCSSTASLI SKRNPCYPYG ILCRTLSQSV KLWQENTSKD
60 70 80 90 100
DSSLNITPRL LKIIPNDTDI VTLEKQDELI KRRRKLSKEV TQMKRLKPVS
110 120 130 140 150
PGLRWYRSPI YPYLYKGRPV RALTVVRKKH GGRNNSGKIT VRHQGGGHRN
160 170 180 190 200
RTRLIDFNRW EGGAQTVQRI EYDPGRSSHI ALLKHNTTGE LSYIIACDGL
210 220 230 240 250
RPGDVVESFR RGIPQTLLNE MGGKVDPAIL SVKTTQRGNC LPISMIPIGT
260 270 280 290 300
IIHNVGITPV GPGKFCRSAG TYARVLAKLP EKKKAIVRLQ SGEHRYVSLE
310 320 330 340 350
AVATIGVVSN IDHQNRSLGK AGRSRWLGIR PTVRGVAMNK CDHPHGGGRG
360 370 380 390
KSKSNKLSMS PWGQLAKGYK TRRGKNQNRM KVKDRPRGKD ARL
Length:393
Mass (Da):43,786
Last modified:October 1, 1993 - v1
Checksum:i6AD955FF61C24923
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18779 Genomic DNA Translation: AAB64992.1
BK006939 Genomic DNA Translation: DAA07604.1
PIRiS30827
RefSeqiNP_010864.3, NM_001178865.3

Genome annotation databases

EnsemblFungiiYEL050C; YEL050C; YEL050C
GeneIDi856660
KEGGisce:YEL050C

Similar proteinsi

Entry informationi

Entry nameiRML2_YEAST
AccessioniPrimary (citable) accession number: P32611
Secondary accession number(s): D3DLK0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

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