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Reviewed, UniProtKB/Swiss-Prot P32611 (RML2_YEAST)

Last modified November 24, 2009. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    54S ribosomal protein RML2, mitochondrial
      Short name=L2
Gene names
Name: RML2
Ordered Locus Names: YEL050C
ORF Names: SYGP-ORF37
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Subunit structure

Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.2

Subcellular location

Mitochondrion.

Miscellaneous

Present with 1600 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the ribosomal protein L2P family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4343Mitochondrion Potential
Chain44 – 39335054S ribosomal protein RML2, mitochondrial
PRO_0000030512

Experimental info

Mutagenesis336 – 3427Missing: Loss of function.
Mutagenesis3431H → Q: Causes a cold-sensitive respiratory growth defect. Does not impair assembly of the ribosomal subunit. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P32611-1 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 6AD955FF61C24923

FASTA39343,786
        10         20         30         40         50         60 
MLVLGSLRSA LSCSSTASLI SKRNPCYPYG ILCRTLSQSV KLWQENTSKD DSSLNITPRL 

        70         80         90        100        110        120 
LKIIPNDTDI VTLEKQDELI KRRRKLSKEV TQMKRLKPVS PGLRWYRSPI YPYLYKGRPV 

       130        140        150        160        170        180 
RALTVVRKKH GGRNNSGKIT VRHQGGGHRN RTRLIDFNRW EGGAQTVQRI EYDPGRSSHI 

       190        200        210        220        230        240 
ALLKHNTTGE LSYIIACDGL RPGDVVESFR RGIPQTLLNE MGGKVDPAIL SVKTTQRGNC 

       250        260        270        280        290        300 
LPISMIPIGT IIHNVGITPV GPGKFCRSAG TYARVLAKLP EKKKAIVRLQ SGEHRYVSLE 

       310        320        330        340        350        360 
AVATIGVVSN IDHQNRSLGK AGRSRWLGIR PTVRGVAMNK CDHPHGGGRG KSKSNKLSMS 

       370        380        390 
PWGQLAKGYK TRRGKNQNRM KVKDRPRGKD ARL 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed: 9169868] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"Functional analysis of ribosomal protein L2 in yeast mitochondria."
Pan C., Mason T.L.
J. Biol. Chem. 272:8165-8171(1997) [PubMed: 9079633] [Abstract]
Cited for: FUNCTION, SUBUNIT, MUTAGENESIS OF 336-VAL--ASP-342 AND HIS-343.
[3]"A yeast strain defective in oleic acid utilization has a mutation in the RML2 gene."
Trotter P.J., Hagerman R.A., Voelker D.R.
Biochim. Biophys. Acta 1438:223-238(1999) [PubMed: 10320805] [Abstract]
Cited for: FUNCTION.
[4]"A mutation in the yeast mitochondrial ribosomal protein Rml2p is associated with a defect in catalase gene expression."
Hagerman R.A., Trotter P.J.
Mol. Cell Biol. Res. Commun. 4:299-306(2001) [PubMed: 11529680] [Abstract]
Cited for: FUNCTION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

U18779 Genomic DNA. Translation: AAB64992.1.
PIRS30827.
RefSeqNP_010864.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:6812N.
IntActP32611. 2 interactions.
STRINGP32611.

Proteomic databases

PeptideAtlasP32611.

Genome annotation databases

EnsemblYEL050C; YEL050C; YEL050C; Saccharomyces cerevisiae. [Genome view]
GeneID856660.
KEGGsce:YEL050C.
NMPDRfig|4932.3.peg.1915.

Organism-specific databases

CYGDYEL050c.
SGDS000000776. RML2.

Phylogenomic databases

HOGENOMP32611.
OMAQRGNCLP
OrthoDBEOG9ZSBK7

Gene expression databases

ArrayExpressP32611.
GenevestigatorP32611.
GermOnlineYEL050C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR002171. Ribosomal_L2.
IPR005880. Ribosomal_L2_bac-type.
IPR014722. Transl_SH3-like_sub.
IPR008991. Translation_prot_SH3-like.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:2.30.30.30. Ribosomal_L2. 1 hit.
PANTHERPTHR13691:SF5. Ribosom_L2_bac. 1 hit.
PTHR13691. Ribosomal_L2. 1 hit.
PfamPF00181. Ribosomal_L2. 1 hit.
PF03947. Ribosomal_L2_C. 1 hit.
[Graphical view]
TIGRFAMsTIGR01171. rplB_bact. 1 hit.
PROSITEPS00467. RIBOSOMAL_L2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio982655.

Entry information

Entry nameRML2_YEAST
AccessionPrimary (citable) accession number: P32611
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 24, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

Ribosomal proteins

Ribosomal proteins families and list of entries

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents