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Protein

V-type proton ATPase subunit D

Gene

VMA8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.

GO - Molecular functioni

  1. proton-transporting ATPase activity, rotational mechanism Source: SGD

GO - Biological processi

  1. hydrogen ion transmembrane transport Source: GOC
  2. vacuolar acidification Source: SGD
Complete GO annotation...

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30169-MONOMER.
ReactomeiREACT_189031. Phagosomal maturation (early endosomal stage).
REACT_189190. Transferrin endocytosis and recycling.
REACT_255567. Insulin receptor recycling.

Protein family/group databases

TCDBi3.A.2.2.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase subunit D
Short name:
V-ATPase subunit D
Alternative name(s):
Vacuolar proton pump subunit D
Gene namesi
Name:VMA8
Ordered Locus Names:YEL051W
ORF Names:SYGP-ORF11
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYEL051w.
SGDiS000000777. VMA8.

Subcellular locationi

GO - Cellular componenti

  1. fungal-type vacuole membrane Source: SGD
  2. vacuolar proton-transporting V-type ATPase, V1 domain Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 256256V-type proton ATPase subunit DPRO_0000144244Add
BLAST

Proteomic databases

MaxQBiP32610.
PaxDbiP32610.
PeptideAtlasiP32610.

Expressioni

Gene expression databases

GenevestigatoriP32610.

Interactioni

Subunit structurei

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d and e). Interacts with RAV1 and RAV2 components of the RAVE complex, which are essential for the stability and assembly of V-ATPase.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RAV1P471042EBI-20264,EBI-25471
VMA7P391115EBI-20264,EBI-20272

Protein-protein interaction databases

BioGridi36678. 206 interactions.
DIPiDIP-2959N.
IntActiP32610. 36 interactions.
MINTiMINT-640736.
STRINGi4932.YEL051W.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4RNDX-ray3.18A/C1-256[»]
ProteinModelPortaliP32610.
SMRiP32610. Positions 10-209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the V-ATPase D subunit family.Curated

Phylogenomic databases

eggNOGiCOG1394.
GeneTreeiENSGT00390000010770.
HOGENOMiHOG000230791.
InParanoidiP32610.
KOiK02149.
OMAiQTAFMIL.
OrthoDBiEOG7PCJTH.

Family and domain databases

InterProiIPR002699. V_ATPase_D.
[Graphical view]
PANTHERiPTHR11671. PTHR11671. 1 hit.
PfamiPF01813. ATP-synt_D. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00309. V_ATPase_subD. 1 hit.

Sequencei

Sequence statusi: Complete.

P32610-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGNREQVFP TRMTLGLMKT KLKGANQGYS LLKRKSEALT KRFRDITKRI
60 70 80 90 100
DDAKQKMGRV MQTAAFSLAE VSYATGENIG YQVQESVSTA RFKVRARQEN
110 120 130 140 150
VSGVYLSQFE SYIDPEINDF RLTGLGRGGQ QVQRAKEIYS RAVETLVELA
160 170 180 190 200
SLQTAFIILD EVIKVTNRRV NAIEHVIIPR TENTIAYINS ELDELDREEF
210 220 230 240 250
YRLKKVQEKK QNETAKLDAE MKLKRDRAEQ DASEVAADEE PQGETLVADQ

EDDVIF
Length:256
Mass (Da):29,194
Last modified:October 1, 1993 - v1
Checksum:iFDE93E1FAF0AEC5B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18779 Genomic DNA. Translation: AAB64991.1.
BK006939 Genomic DNA. Translation: DAA07603.1.
PIRiS30826.
RefSeqiNP_010863.1. NM_001178866.1.

Genome annotation databases

EnsemblFungiiYEL051W; YEL051W; YEL051W.
GeneIDi856659.
KEGGisce:YEL051W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18779 Genomic DNA. Translation: AAB64991.1.
BK006939 Genomic DNA. Translation: DAA07603.1.
PIRiS30826.
RefSeqiNP_010863.1. NM_001178866.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4RNDX-ray3.18A/C1-256[»]
ProteinModelPortaliP32610.
SMRiP32610. Positions 10-209.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36678. 206 interactions.
DIPiDIP-2959N.
IntActiP32610. 36 interactions.
MINTiMINT-640736.
STRINGi4932.YEL051W.

Protein family/group databases

TCDBi3.A.2.2.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Proteomic databases

MaxQBiP32610.
PaxDbiP32610.
PeptideAtlasiP32610.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYEL051W; YEL051W; YEL051W.
GeneIDi856659.
KEGGisce:YEL051W.

Organism-specific databases

CYGDiYEL051w.
SGDiS000000777. VMA8.

Phylogenomic databases

eggNOGiCOG1394.
GeneTreeiENSGT00390000010770.
HOGENOMiHOG000230791.
InParanoidiP32610.
KOiK02149.
OMAiQTAFMIL.
OrthoDBiEOG7PCJTH.

Enzyme and pathway databases

BioCyciYEAST:G3O-30169-MONOMER.
ReactomeiREACT_189031. Phagosomal maturation (early endosomal stage).
REACT_189190. Transferrin endocytosis and recycling.
REACT_255567. Insulin receptor recycling.

Miscellaneous databases

NextBioi982652.
PROiP32610.

Gene expression databases

GenevestigatoriP32610.

Family and domain databases

InterProiIPR002699. V_ATPase_D.
[Graphical view]
PANTHERiPTHR11671. PTHR11671. 1 hit.
PfamiPF01813. ATP-synt_D. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00309. V_ATPase_subD. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "VMA8 encodes a 32-kDa V1 subunit of the Saccharomyces cerevisiae vacuolar H(+)-ATPase required for function and assembly of the enzyme complex."
    Graham L.A., Hill K.J., Stevens T.H.
    J. Biol. Chem. 270:15037-15044(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 6-12; 170-179 AND 182-187.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A bovine cDNA and a yeast gene (VMA8) encoding the subunit D of the vacuolar H(+)-ATPase."
    Nelson H., Mandiyan S., Nelson N.
    Proc. Natl. Acad. Sci. U.S.A. 92:497-501(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly."
    Seol J.H., Shevchenko A., Shevchenko A., Deshaies R.J.
    Nat. Cell Biol. 3:384-391(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAV1 AND RAV2.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVATD_YEAST
AccessioniPrimary (citable) accession number: P32610
Secondary accession number(s): D3DLJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: February 4, 2015
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.