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P32610 (VATD_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
V-type proton ATPase subunit D

Short name=V-ATPase subunit D
Alternative name(s):
Vacuolar proton pump subunit D
Gene names
Name:VMA8
Ordered Locus Names:YEL051W
ORF Names:SYGP-ORF11
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length256 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.

Subunit structure

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d and e). Interacts with RAV1 and RAV2 components of the RAVE complex, which are essential for the stability and assembly of V-ATPase. Ref.5

Miscellaneous

Present with 8500 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the V-ATPase D subunit family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RAV1P471042EBI-20264,EBI-25471
VMA7P391115EBI-20264,EBI-20272

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 256256V-type proton ATPase subunit D
PRO_0000144244

Sequences

Sequence LengthMass (Da)Tools
P32610 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: FDE93E1FAF0AEC5B

FASTA25629,194
        10         20         30         40         50         60 
MSGNREQVFP TRMTLGLMKT KLKGANQGYS LLKRKSEALT KRFRDITKRI DDAKQKMGRV 

        70         80         90        100        110        120 
MQTAAFSLAE VSYATGENIG YQVQESVSTA RFKVRARQEN VSGVYLSQFE SYIDPEINDF 

       130        140        150        160        170        180 
RLTGLGRGGQ QVQRAKEIYS RAVETLVELA SLQTAFIILD EVIKVTNRRV NAIEHVIIPR 

       190        200        210        220        230        240 
TENTIAYINS ELDELDREEF YRLKKVQEKK QNETAKLDAE MKLKRDRAEQ DASEVAADEE 

       250 
PQGETLVADQ EDDVIF 

« Hide

References

« Hide 'large scale' references
[1]"VMA8 encodes a 32-kDa V1 subunit of the Saccharomyces cerevisiae vacuolar H(+)-ATPase required for function and assembly of the enzyme complex."
Graham L.A., Hill K.J., Stevens T.H.
J. Biol. Chem. 270:15037-15044(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 6-12; 170-179 AND 182-187.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A bovine cDNA and a yeast gene (VMA8) encoding the subunit D of the vacuolar H(+)-ATPase."
Nelson H., Mandiyan S., Nelson N.
Proc. Natl. Acad. Sci. U.S.A. 92:497-501(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly."
Seol J.H., Shevchenko A., Shevchenko A., Deshaies R.J.
Nat. Cell Biol. 3:384-391(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RAV1 AND RAV2.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18779 Genomic DNA. Translation: AAB64991.1.
BK006939 Genomic DNA. Translation: DAA07603.1.
PIRS30826.
RefSeqNP_010863.1. NM_001178866.1.

3D structure databases

ProteinModelPortalP32610.
SMRP32610. Positions 10-209.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36678. 204 interactions.
DIPDIP-2959N.
IntActP32610. 36 interactions.
MINTMINT-640736.
STRING4932.YEL051W.

Protein family/group databases

TCDB3.A.2.2.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Proteomic databases

PaxDbP32610.
PeptideAtlasP32610.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYEL051W; YEL051W; YEL051W.
GeneID856659.
KEGGsce:YEL051W.

Organism-specific databases

CYGDYEL051w.
SGDS000000777. VMA8.

Phylogenomic databases

eggNOGCOG1394.
GeneTreeENSGT00390000010770.
HOGENOMHOG000230791.
KOK02149.
OMAQSKNIMG.
OrthoDBEOG7PCJTH.

Enzyme and pathway databases

BioCycYEAST:G3O-30169-MONOMER.

Gene expression databases

GenevestigatorP32610.

Family and domain databases

InterProIPR002699. V_ATPase_D.
[Graphical view]
PANTHERPTHR11671. PTHR11671. 1 hit.
PfamPF01813. ATP-synt_D. 1 hit.
[Graphical view]
TIGRFAMsTIGR00309. V_ATPase_subD. 1 hit.
ProtoNetSearch...

Other

NextBio982652.
PROP32610.

Entry information

Entry nameVATD_YEAST
AccessionPrimary (citable) accession number: P32610
Secondary accession number(s): D3DLJ9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: April 16, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families