P32610 (VATD_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
V-type proton ATPase subunit D
Short name=V-ATPase subunit D

Alternative name(s):
Vacuolar proton pump subunit D

Gene names

Name:	VMA8
Ordered Locus Names:	YEL051W
ORF Names:	SYGP-ORF11

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system.
Subunit structure	V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d and e). Interacts with RAV1 and RAV2 components of the RAVE complex, which are essential for the stability and assembly of V-ATPase. Ref.5
Miscellaneous	Present with 8500 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the V-ATPase D subunit family.

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ATP hydrolysis coupled proton transport Inferred from electronic annotation. Source: InterPro vacuolar acidification Traceable author statement. Source: SGD
Cellular component	fungal-type vacuole membrane Traceable author statement. Source: SGD vacuolar proton-transporting V-type ATPase, V1 domain Traceable author statement. Source: SGD
Molecular function	protein binding Inferred from physical interaction Ref.5. Source: IntAct proton-transporting ATPase activity, rotational mechanism Traceable author statement. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RAV1	P47104	2	EBI-20264,EBI-25471
VMA13	P41807	3	EBI-20264,EBI-20281
VMA7	P39111	5	EBI-20264,EBI-20272

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	V-type proton ATPase subunit D		PRO_0000144244

Sequences

	Sequence		Length	Mass (Da)
	P32610 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: FDE93E1FAF0AEC5B	FASTA	256	29,194
	10 20 30 40 50 60 MSGNREQVFP TRMTLGLMKT KLKGANQGYS LLKRKSEALT KRFRDITKRI DDAKQKMGRV 70 80 90 100 110 120 MQTAAFSLAE VSYATGENIG YQVQESVSTA RFKVRARQEN VSGVYLSQFE SYIDPEINDF 130 140 150 160 170 180 RLTGLGRGGQ QVQRAKEIYS RAVETLVELA SLQTAFIILD EVIKVTNRRV NAIEHVIIPR 190 200 210 220 230 240 TENTIAYINS ELDELDREEF YRLKKVQEKK QNETAKLDAE MKLKRDRAEQ DASEVAADEE 250 PQGETLVADQ EDDVIF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"VMA8 encodes a 32-kDa V1 subunit of the Saccharomyces cerevisiae vacuolar H(+)-ATPase required for function and assembly of the enzyme complex." Graham L.A., Hill K.J., Stevens T.H. J. Biol. Chem. 270:15037-15044(1995) [PubMed: 7797485] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 6-12; 170-179 AND 182-187.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A bovine cDNA and a yeast gene (VMA8) encoding the subunit D of the vacuolar H(+)-ATPase." Nelson H., Mandiyan S., Nelson N. Proc. Natl. Acad. Sci. U.S.A. 92:497-501(1995) [PubMed: 7831318] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly." Seol J.H., Shevchenko A., Shevchenko A., Deshaies R.J. Nat. Cell Biol. 3:384-391(2001) [PubMed: 11283612] [Abstract] Cited for: INTERACTION WITH RAV1 AND RAV2.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18779 Genomic DNA. Translation: AAB64991.1. BK006939 Genomic DNA. Translation: DAA07603.1.
PIR	S30826.
RefSeq	NP_010863.1. NM_001178866.1.
3D structure databases
ProteinModelPortal	P32610.
SMR	P32610. Positions 10-197.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2959N.
IntAct	P32610. 46 interactions.
MINT	MINT-640736.
STRING	P32610.
Protein family/group databases
TCDB	3.A.2.2.3. H+- or Na+-translocating F-type, V-type and A-type ATPase (F-ATPase) superfamily.
Proteomic databases
PeptideAtlas	P32610.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YEL051W; YEL051W; YEL051W.
GeneID	856659.
KEGG	sce:YEL051W.
NMPDR	fig|4932.3.peg.1913.
Organism-specific databases
CYGD	YEL051w.
SGD	S000000777. VMA8.
Phylogenomic databases
eggNOG	fuNOG04947.
GeneTree	EFGT00050000004465.
HOGENOM	HBG732046.
OMA	IIPRIEN.
OrthoDB	EOG4936T0.
Gene expression databases
ArrayExpress	P32610.
Genevestigator	P32610.
GermOnline	YEL051W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002699. ATPase_V1/A1-cplx_dsu. [Graphical view]
KO	K02149.
PANTHER	PTHR11671. ATPase_V1/A1_D. 1 hit.
Pfam	PF01813. ATP-synt_D. 1 hit. [Graphical view]
TIGRFAMs	TIGR00309. V_ATPase_subD. 1 hit.
ProtoNet	Search...
Other
NextBio	982652.

Entry information

Entry name

VATD_YEAST

Accession

Primary (citable) accession number: P32610
Secondary accession number(s): D3DLJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	January 25, 2012
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program