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Protein

Vacuolar segregation protein PEP7

Gene

PEP7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for vacuole segregation and vacuole protein sorting. Possibly part of a complex which tethers the vacuole membrane to microtubules, either directly or via kinesin or dynein-like motor proteins. Probably functions in several interorganelle traffic pathways.2 Publications

Miscellaneous

Present with 768 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri6 – 29C2H2-typeAdd BLAST24
Zinc fingeri72 – 137FYVE-type 1PROSITE-ProRule annotationAdd BLAST66
Zinc fingeri215 – 297FYVE-type 2PROSITE-ProRule annotationAdd BLAST83

GO - Molecular functioni

GO - Biological processi

  • endocytosis Source: GO_Central
  • endosomal vesicle fusion Source: GO_Central
  • Golgi to endosome transport Source: SGD
  • Golgi to vacuole transport Source: SGD
  • vacuole inheritance Source: SGD
  • vesicle docking involved in exocytosis Source: SGD
  • vesicle fusion Source: SGD

Keywordsi

LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29880-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar segregation protein PEP7
Alternative name(s):
Carboxypeptidase Y-deficient protein 7
Protein VAC1
Vacuolar protein sorting-associated protein 19
Vacuolar protein-targeting protein 19
Gene namesi
Name:PEP7
Synonyms:VAC1, VPL21, VPS19, VPT19
Ordered Locus Names:YDR323C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR323C
SGDiS000002731 PEP7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000468581 – 515Vacuolar segregation protein PEP7Add BLAST515

Proteomic databases

MaxQBiP32609
PaxDbiP32609
PRIDEiP32609

Interactioni

Subunit structurei

Interacts with VPS21, VPS45, PEP3 and PEP5.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
VPS45P389323EBI-20208,EBI-20444

GO - Molecular functioni

Protein-protein interaction databases

BioGridi32381, 150 interactors
DIPiDIP-5240N
IntActiP32609, 6 interactors
STRINGi4932.YDR323C

Structurei

3D structure databases

ProteinModelPortaliP32609
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri6 – 29C2H2-typeAdd BLAST24
Zinc fingeri72 – 137FYVE-type 1PROSITE-ProRule annotationAdd BLAST66
Zinc fingeri215 – 297FYVE-type 2PROSITE-ProRule annotationAdd BLAST83

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOGENOMiHOG000001058
InParanoidiP32609
KOiK12481
OMAiQLNRHID
OrthoDBiEOG092C2B59

Family and domain databases

Gene3Di3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR036531 Rbsn_Rab-bd_sf
IPR021565 Rbsn_Rab_binding_dom
IPR013087 Znf_C2H2_type
IPR000306 Znf_FYVE
IPR017455 Znf_FYVE-rel
IPR011011 Znf_FYVE_PHD
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF01363 FYVE, 1 hit
PF11464 Rbsn, 1 hit
SMARTiView protein in SMART
SM00064 FYVE, 2 hits
SUPFAMiSSF140125 SSF140125, 1 hit
SSF57903 SSF57903, 2 hits
PROSITEiView protein in PROSITE
PS50178 ZF_FYVE, 2 hits
PS00028 ZINC_FINGER_C2H2_1, 1 hit

Sequencei

Sequence statusi: Complete.

P32609-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLENVSCPI CLRKFDNLQA LNAHLDVEHG FNDNEDSLGS NDSRLVNGKQ
60 70 80 90 100
KKARSVDSSA QKLKRSHWEK FKKGKSCCHT CGRTLNNNIG AINCRKCGKL
110 120 130 140 150
YCRRHLPNMI KLNLSAQYDP RNGKWYNCCH DCFVTKPGYN DYGEVIDLTP
160 170 180 190 200
EFFKVRNIKR EDKNLRLLQL ENRFVRLVDG LITLYNTYSR SIIHNLKMNS
210 220 230 240 250
EMSKLERTVT PWRDDRSVLF CNICSEPFGL LLRKHHCRLC GMVVCDDANR
260 270 280 290 300
NCSNEISIGY LMSAASDLPF EYNIQKDDLL HIPISIRLCS HCIDMLFIGR
310 320 330 340 350
KFNKDVRMPL SGIFAKYDSM QNISKVIDSL LPIFEDSLNS LKVETAKDSE
360 370 380 390 400
NTLDPKNLND LARLRHKLLN SFNLYNTLTR QLLSVEPQSH LERQLQNSIK
410 420 430 440 450
IASAAYINEK ILPLKSLPAI LNPEGHKTNE DGQKAEPEVK KLSQLMIENL
460 470 480 490 500
TIKEVKELRE ELMVLKEQSY LIESTIQDYK KQRRLEEIVT LNKNLEELHS
510
RIHTVQSKLG DHGFN
Length:515
Mass (Da):59,443
Last modified:September 27, 2004 - v2
Checksum:i53C304F1ADA5B7A1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti366H → Y in AAA35203 (PubMed:1730622).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M80596 Genomic DNA Translation: AAA35203.1
U22070 Genomic DNA Translation: AAB60290.1
U32517 Genomic DNA Translation: AAB64759.1
BK006938 Genomic DNA Translation: DAA12166.1
PIRiS59811
RefSeqiNP_010610.3, NM_001180631.3

Genome annotation databases

EnsemblFungiiYDR323C; YDR323C; YDR323C
GeneIDi851923
KEGGisce:YDR323C

Similar proteinsi

Entry informationi

Entry nameiPEP7_YEAST
AccessioniPrimary (citable) accession number: P32609
Secondary accession number(s): D6VSV6, Q99229
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: September 27, 2004
Last modified: May 23, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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