P32608 (RTG2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Retrograde regulation protein 2

Gene names

Name:	RTG2
Ordered Locus Names:	YGL252C
ORF Names:	NRF588

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for a novel path of interorganelle communication between mitochondria, peroxisomes and the nucleus, thereby maintaining a functional metabolic interaction between the tricarboxylic acid and glyoxylate cycles. In particular, required for the retrograde expression of the peroxisomal isoform of citrate synthase, CIT2.
Miscellaneous	Present with 3260 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the gppA/ppx family.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	extrachromosomal rDNA circle accumulation involved in replicative cell aging Inferred from mutant phenotype. Source: SGD intracellular signal transduction Inferred from mutant phenotype. Source: SGD mitochondria-nucleus signaling pathway Inferred from mutant phenotype Ref.1. Source: SGD
Cellular component	SLIK (SAGA-like) complex Inferred from direct assay. Source: SGD cytoplasm Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SPT7	P35177	2	EBI-16322,EBI-17958

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 588	588	Retrograde regulation protein 2		PRO_0000194312
Amino acid modifications
	Modified residue	5	1	Phosphoserine Ref.8
	Modified residue	7	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P32608 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 050DBCA65E38666F	FASTA	588	65,572
	10 20 30 40 50 60 MSTLSDSDTE TEVVSRNLCG IVDIGSNGIR FSISSKAAHH ARIMPCVFKD RVGLSLYEVQ 70 80 90 100 110 120 YNTHTNAKCP IPRDIIKEVC SAMKRFKLIC DDFGVPETSV RVIATEATRD AINADEFVNA 130 140 150 160 170 180 VYGSTGWKVE ILGQEDETRV GIYGVVSSFN TVRGLYLDVA GGSTQLSWVI SSHGEVKQSS 190 200 210 220 230 240 KPVSLPYGAG TLLRRMRTDD NRALFYEIKE AYKDAIEKIG IPQEMIDDAK KEGGFDLWTR 250 260 270 280 290 300 GGGLRGMGHL LLYQSEGYPI QTIINGYACT YEEFSSMSDY LFLKQKIPGS SKEHKIFKVS 310 320 330 340 350 360 DRRALQLPAV GLFMSAVFEA IPQIKAVHFS EGGVREGSLY SLLPKEIRAQ DPLLIASRPY 370 380 390 400 410 420 APLLTEKYLY LLRTSIPQED IPEIVNERIA PALCNLAFVH ASYPKELQPT AALHVATRGI 430 440 450 460 470 480 IAGCHGLSHR ARALIGIALC SRWGGNIPES EEKYSQELEQ VVLREGDKAE ALRIVWWTKY 490 500 510 520 530 540 IGTIMYVICG VHPGGNIRDN VFDFHVSKRS EVETSLKELI IDDANTTKVK EESTRKNRGY 550 560 570 580 EVVVRISKDD LKTSASVRSR IITLQKKVRK LSRGSVERVK IGVQFYEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"RTG1 and RTG2: two yeast genes required for a novel path of communication from mitochondria to the nucleus." Liao X., Butow R.A. Cell 72:61-71(1993) [PubMed: 8422683] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Jia Y., Etherige J., Shyjan A. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: COP161U7.
[3]	"Sequence of a 39,411 bp DNA fragment covering the left end of chromosome VII of Saccharomyces cerevisiae." Coissac E., Maillier E., Robineau S., Netter P. Yeast 12:1555-1562(1996) [PubMed: 8972578] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5 AND SER-7, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M97691 Genomic DNA. Translation: AAA96809.1. X94357 Genomic DNA. Translation: CAA64135.1. Z72774 Genomic DNA. Translation: CAA96972.1. AY692809 Genomic DNA. Translation: AAT92828.1. BK006941 Genomic DNA. Translation: DAA07867.1.
PIR	S61609.
RefSeq	NP_011262.1. NM_001181118.1.
3D structure databases
ProteinModelPortal	P32608.
SMR	P32608. Positions 18-348.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6826N.
IntAct	P32608. 18 interactions.
MINT	MINT-638108.
STRING	P32608.
Proteomic databases
PeptideAtlas	P32608.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL252C; YGL252C; YGL252C.
GeneID	852640.
KEGG	sce:YGL252C.
NMPDR	fig|4932.3.peg.2365.
Organism-specific databases
CYGD	YGL252c.
SGD	S000003221. RTG2.
Phylogenomic databases
eggNOG	fuNOG07656.
GeneTree	EFGT00050000005451.
HOGENOM	HBG325605.
OMA	GSNGIRF.
OrthoDB	EOG418FX7.
Gene expression databases
ArrayExpress	P32608.
Genevestigator	P32608.
GermOnline	YGL252C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003695. Ppx_GppA. [Graphical view]
KO	K11369.
Pfam	PF02541. Ppx-GppA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971891.

Entry information

Entry name

RTG2_YEAST

Accession

Primary (citable) accession number: P32608
Secondary accession number(s): D6VV83

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program