Retrograde regulation protein 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P32607 (RTG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Retrograde regulation protein 1

Gene names

Name:	RTG1
Ordered Locus Names:	YOL067C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for a novel path of interorganelle communication between mitochondria, peroxisomes and the nucleus, thereby maintaining a functional metabolic interaction between the tricarboxylic acid and glyoxylate cycles. Transcription factor that regulates CIT2 gene expression. Binds to two identical sites oriented as inverted repeats 28 bp apart in a regulatory upstream activation sequence element (UASR) in the CIT2 promoter. The core binding site is 5'-GGTCAC-3'.
Subunit structure	Binds DNA as a heterodimer with RTG3.
Subcellular location	Nucleus Probable.
Miscellaneous	Present with 2190 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to oleic acid Inferred from mutant phenotype PubMed 12393187. Source: SGD mitochondria-nucleus signaling pathway Inferred from direct assay PubMed 7493987. Source: SGD positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 7493987. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 10848632. Source: SGD nucleus Inferred from direct assay PubMed 10848632. Source: SGD
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from mutant phenotype PubMed 7493987 Ref.1. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RTG3	P38165	4	EBI-16312,EBI-16328

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 177

177

Retrograde regulation protein 1

PRO_0000127433

Regions

Domain

11 – 96

bHLH

Amino acid modifications

Modified residue

Phosphoserine Ref.7

Modified residue

Phosphothreonine Ref.7

Modified residue

164

Phosphoserine Ref.7

Modified residue

177

Phosphoserine Ref.7

Experimental info

Sequence conflict

105 – 113

REEVELMVK → KRGGGTDGE Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P32607 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 396A6C3A34C49E50
Show »

FASTA

177

19,016

        10         20         30         40         50         60 
MSSIPAGTDP GSCGANFKND RKRRDKINDR IQELLSIIPK DFFRDYYGNS GSNDTLSEST 

        70         80         90        100        110        120 
PGALGLSSKA KGTGTKDGKP NKGQILTQAV EYISHLQNQV DTQNREEVEL MVKATQLAKQ 

       130        140        150        160        170 
TGTIVNDINL ENTSAEVALS RIGVGPLAAT NDDSVRPPAK RLSSFEYGGY GEYGNGS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"RTG1 and RTG2: two yeast genes required for a novel path of communication from mitochondria to the nucleus." Liao X., Butow R.A. Cell 72:61-71(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of a 33.2 kb segment from the left arm of yeast chromosome XV reveals eight known genes and ten new open reading frames including homologues of ABC transporters, inositol phosphatases and human expressed sequence tags." Tzermia M., Katsoulou C., Alexandraki D. Yeast 13:583-589(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52; THR-55; SER-164 AND SER-177, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M97690 Genomic DNA. No translation available. Z74809 Genomic DNA. Translation: CAA99077.1. AY692940 Genomic DNA. Translation: AAT92959.1. BK006948 Genomic DNA. Translation: DAA10716.1.
PIR	S66760.
RefSeq	NP_014574.1. NM_001183322.1.
3D structure databases
ProteinModelPortal	P32607.
SMR	P32607. Positions 15-103.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2500N.
IntAct	P32607. 12 interactions.
MINT	MINT-2786078.
STRING	4932.YOL067C.
Proteomic databases
PaxDb	P32607.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOL067C; YOL067C; YOL067C.
GeneID	854087.
KEGG	sce:YOL067C.
Organism-specific databases
CYGD	YOL067c.
SGD	S000005428. RTG1.
Phylogenomic databases
eggNOG	NOG42107.
HOGENOM	HOG000141752.
OMA	YISHLQN.
OrthoDB	EOG43JGFG.
Gene expression databases
Genevestigator	P32607.
GermOnline	YOL067C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	4.10.280.10. 2 hits.
InterPro	IPR011598. bHLH_dom. [Graphical view]
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. BHLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975734.

Entry information

Entry name

RTG1_YEAST

Accession

Primary (citable) accession number: P32607
Secondary accession number(s): D6W200, Q08228

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents