P32605 (RU1A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
U1 small nuclear ribonucleoprotein A
Short name=U1 snRNP A
Short name=U1-A
Short name=U1A

Alternative name(s):
Mutant U1 die protein 1

Gene names

Name:	MUD1
Ordered Locus Names:	YBR119W
ORF Names:	YBR0915

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in nuclear mRNA splicing. The principal role of the U1A is to help fold or maintain U1 RNA in an active configuration. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. Ref.1 Ref.5
Subunit structure	Component of the spliceosome where it is associated with snRNP U1.
Subcellular location	Nucleus By similarity Ref.8.
Miscellaneous	Present with 2950 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Belongs to the RRM U1 A/B'' family. Contains 2 RRM (RNA recognition motif) domains.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus Spliceosome
Domain	Repeat
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nuclear mRNA splicing, via spliceosome Inferred from genetic interaction Ref.1. Source: SGD
Cellular component	U1 snRNP Inferred from direct assay. Source: SGD U2-type prespliceosome Inferred from direct assay. Source: SGD
Molecular function	U1 snRNA binding Inferred from physical interaction Ref.5. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	U1 small nuclear ribonucleoprotein A		PRO_0000081891
Regions
	Domain	2 – 113	112	RRM 1
	Domain	227 – 298	72	RRM 2
Experimental info
	Mutagenesis	4	1	L → I: Sensitive in DMS protection of U1 snRNA; when associated with 6-I-N-7. Ref.5
	Mutagenesis	6 – 7	2	FQ → IN: Sensitive in DMS protection of U1 snRNA; when associated with I-4.
	Mutagenesis	63	1	L → I: Sensitive in DMS protection of U1 snRNA; when associated with 65-D--L-67. Ref.5
	Mutagenesis	65 – 67	3	VSI → DIL: Sensitive in DMS protection of U1 snRNA; when associated with I-63. Ref.5
	Mutagenesis	69 – 72	4	RSSK → LKTM: Sensitive in DMS protection of U1 snRNA. Ref.5
	Mutagenesis	74 – 75	2	TN → RG: Sensitive in DMS protection of U1 snRNA; when associated with 79-V-I-80.
	Mutagenesis	79 – 80	2	LT → VI: Sensitive in DMS protection of U1 snRNA; when associated with 74-R-G-75.
	Mutagenesis	228	1	V → I: Splicing defects; when associated with 230-F--T-232. Ref.5
	Mutagenesis	230 – 232	3	LIQ → FLT: Splicing defects; when associated with I-228. Ref.5
	Mutagenesis	258 – 260	3	VSV → PGH: Splicing defects. Ref.5
	Mutagenesis	262 – 263	2	NL → DI: Splicing defects; when associated with F-268.
	Mutagenesis	268	1	Y → F: Splicing defects; when associated with 262-D-I-263. Ref.5
	Mutagenesis	291 – 293	3	DVT → AMK: Splicing defects; when associated with S-295. Ref.5
	Mutagenesis	295	1	G → S: Splicing defects; when associated with 291-A--K-293. Ref.5
	Sequence conflict	1 – 3	3	MSA → MTNKNR in CAA55621. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P32605 [UniParc]. Last modified October 1, 1993. Version 1. Checksum: 42256530BF1C5379	FASTA	298	34,378
	10 20 30 40 50 60 MSALYFQNLP SRPANKENYT RLLLKHINPN NKYAINPSLP LPHNKLQISS QPLMLLDDQM 70 80 90 100 110 120 GLLEVSISRS SKMTNQAFLT FVTQEEADRF LEKYTTTALK VQGRKVRMGK ARTNSLLGLS 130 140 150 160 170 180 IEMQKKKGND ETYNLDIKKV LKARKLKRKL RSDDICAKKF RLKRQIRRLK HKLRSRKVEE 190 200 210 220 230 240 AEIDRIVKEF ETRRLENMKS QQENLKQSQK PLKRAKVSNT MENPPNKVLL IQNLPSGTTE 250 260 270 280 290 QLLSQILGNE ALVEIRLVSV RNLAFVEYET VADATKIKNQ LGSTYKLQNN DVTIGFAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An enhancer screen identifies a gene that encodes the yeast U1 snRNP A protein: implications for snRNP protein function in pre-mRNA splicing." Liao X.C., Tang J., Rosbash M. Genes Dev. 7:419-428(1993) [PubMed: 8449403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH U1 SNRNA. Strain: XLY219.
[2]	"Analysis of a 70 kb region on the right arm of yeast chromosome II." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 10:1363-1381(1994) [PubMed: 7900426] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Characterization of yeast U1 snRNP A protein: identification of the N-terminal RNA binding domain (RBD) binding site and evidence that the C-terminal RBD functions in splicing." Tang J., Rosbash M. RNA 2:1058-1070(1996) [PubMed: 8849781] [Abstract] Cited for: FUNCTION, U1 SNRNA BINDING, MUTAGENESIS OF LEU-4; 6-PHE-GLN-7; LEU-63; 65-VAL--ILE-67; 69-ARG--LYS-72; 74-THR-ASN-75; 79-LEU-THR-80; VAL-228; 230-LEU--GLN-232; 258-VAL--VAL-260; 262-ASN-LEU-263; TYR-268; 291-ASP--THR-293 AND GLY-295.
[6]	"Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometry." Neubauer G., Gottschalk A., Fabrizio P., Seraphin B., Luehrmann R., Mann M. Proc. Natl. Acad. Sci. U.S.A. 94:385-390(1997) [PubMed: 9012791] [Abstract] Cited for: IDENTIFICATION IN U1 SNRNP BY MASS SPECTROMETRY.
[7]	"Composition and functional characterization of the yeast spliceosomal penta-snRNP." Stevens S.W., Ryan D.E., Ge H.Y., Moore R.E., Young M.K., Lee T.D., Abelson J. Mol. Cell 9:31-44(2002) [PubMed: 11804584] [Abstract] Cited for: IDENTIFICATION IN U1 SNRNP BY MASS SPECTROMETRY.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71061 Genomic DNA. Translation: CAA50378.1. X78993 Genomic DNA. Translation: CAA55621.1. Z35988 Genomic DNA. Translation: CAA85076.1. BK006936 Genomic DNA. Translation: DAA07237.1.
PIR	A46301.
RefSeq	NP_009677.1. NM_001178467.1.
3D structure databases
ProteinModelPortal	P32605.
SMR	P32605. Positions 26-124, 219-298.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-223N.
IntAct	P32605. 14 interactions.
MINT	MINT-525465.
STRING	P32605.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR119W; YBR119W; YBR119W.
GeneID	852416.
KEGG	sce:YBR119W.
NMPDR	fig|4932.3.peg.378.
Organism-specific databases
CYGD	YBR119w.
SGD	S000000323. MUD1.
Phylogenomic databases
eggNOG	fuNOG11707.
OMA	FESHELA.
OrthoDB	EOG4TB7MX.
Gene expression databases
ArrayExpress	P32605.
Genevestigator	P32605.
GermOnline	YBR119W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. IPR024888. U1_snRNP_A/U2_snRNP_B''. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 2 hits.
PANTHER	PTHR10501. PTHR10501. 1 hit.
Pfam	PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00360. RRM. 2 hits. [Graphical view]
PROSITE	PS50102. RRM. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	971273.

Entry information

Entry name

RU1A_YEAST

Accession

Primary (citable) accession number: P32605
Secondary accession number(s): D6VQB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	January 25, 2012
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program