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Protein

Fructose-2,6-bisphosphatase

Gene

FBP26

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This is predominantly if not solely a fructose-2,6-bisphosphatase.

Miscellaneous

Present with 1680 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei53Fructose 6-phosphateBy similarity1
Binding sitei78Fructose 6-phosphateBy similarity1
Active sitei104Sequence analysis1
Binding sitei106Fructose 6-phosphateBy similarity1
Binding sitei112Fructose 6-phosphateBy similarity1
Binding sitei169Fructose 6-phosphateBy similarity1
Binding sitei173Fructose 6-phosphateBy similarity1
Binding sitei231Fructose 2,6-bisphosphateBy similarity1
Sitei231Transition state stabilizerBy similarity1
Active sitei232Tele-phosphohistidine intermediateBy similarity1
Binding sitei238Fructose 2,6-bisphosphateBy similarity1
Sitei238Transition state stabilizerBy similarity1
Binding sitei244Fructose 2,6-bisphosphate; via amide nitrogenBy similarity1
Active sitei302Proton donor/acceptorBy similarity1
Binding sitei313Fructose 2,6-bisphosphateBy similarity1
Binding sitei327Fructose 2,6-bisphosphateBy similarity1
Binding sitei331Fructose 2,6-bisphosphateBy similarity1
Binding sitei342Fructose 2,6-bisphosphateBy similarity1
Sitei367Transition state stabilizerBy similarity1
Binding sitei368Fructose 2,6-bisphosphateBy similarity1
Binding sitei372Fructose 2,6-bisphosphateBy similarity1
Binding sitei404ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 28ATPBy similarity9
Nucleotide bindingi143 – 148ATPBy similarity6
Nucleotide bindingi324 – 327ATPBy similarity4
Nucleotide bindingi368 – 372ATPBy similarity5

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • fructose-2,6-bisphosphate 2-phosphatase activity Source: SGD

GO - Biological processi

  • fructose 2,6-bisphosphate metabolic process Source: InterPro
  • fructose metabolic process Source: InterPro
  • glucose metabolic process Source: SGD

Keywordsi

Molecular functionHydrolase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YJL155C-MONOMER
ReactomeiR-SCE-70171 Glycolysis

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-2,6-bisphosphatase (EC:3.1.3.46)
Gene namesi
Name:FBP26
Ordered Locus Names:YJL155C
ORF Names:J0575
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL155C
SGDiS000003691 FBP26

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001799751 – 452Fructose-2,6-bisphosphataseAdd BLAST452

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei435PhosphoserineCombined sources1
Modified residuei446PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32604
PaxDbiP32604
PRIDEiP32604

PTM databases

iPTMnetiP32604

Expressioni

Inductioni

Inhibited by fructose 6-P, activated by glycerol 3-P.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
YLR345WQ061373EBI-6749,EBI-33827

Protein-protein interaction databases

BioGridi33605, 84 interactors
DIPiDIP-2593N
IntActiP32604, 6 interactors
MINTiP32604
STRINGi4932.YJL155C

Structurei

3D structure databases

ProteinModelPortaliP32604
SMRiP32604
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 2236-phosphofructo-2-kinaseBy similarityAdd BLAST223
Regioni224 – 452Fructose-2,6-bisphosphataseAdd BLAST229

Sequence similaritiesi

In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000018751
HOGENOMiHOG000181112
InParanoidiP32604
KOiK19029
OMAiMKRINCY
OrthoDBiEOG092C2SJU

Family and domain databases

CDDicd07067 HP_PGM_like, 1 hit
Gene3Di3.40.50.1240, 1 hit
InterProiView protein in InterPro
IPR003094 6Pfruct_kin
IPR013079 6Phosfructo_kin
IPR013078 His_Pase_superF_clade-1
IPR029033 His_PPase_superfam
IPR027417 P-loop_NTPase
IPR001345 PG/BPGM_mutase_AS
PANTHERiPTHR10606 PTHR10606, 1 hit
PfamiView protein in Pfam
PF01591 6PF2K, 1 hit
PF00300 His_Phos_1, 1 hit
PRINTSiPR00991 6PFRUCTKNASE
SMARTiView protein in SMART
SM00855 PGAM, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
SSF53254 SSF53254, 1 hit
PROSITEiView protein in PROSITE
PS00175 PG_MUTASE, 1 hit

Sequencei

Sequence statusi: Complete.

P32604-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGYSTISNDN DIKVCVIMVG LPARGKSFIS QKIIRYLSWL SIKAKCFNVG
60 70 80 90 100
NYRRDVSGNV PMDAEFFNFE NTDNFKLREL AAQNAIKDIV NFFTKEDGSV
110 120 130 140 150
AVFDATNSTR KRRKWLKDIC EKNNIQPMFL ESWSNDHELI INNAKDIGST
160 170 180 190 200
SPDYENSEPH VAEADFLERI RQYERFYEPL DPQKDKDMTF IKLVNIIEEV
210 220 230 240 250
VINKIRTYLE SRIVFYVMNI RPKPKYIWLS RHGESIYNVE KKIGGDSSLS
260 270 280 290 300
ERGFQYAKKL EQLVKESAGE INLTVWTSTL KRTQQTANYL PYKKLQWKAL
310 320 330 340 350
DELDAGVCDG MTYEEIEKEY PEDFKARDND KYEYRYRGGE SYRDVVIRLE
360 370 380 390 400
PVIMELERQE NVLIITHQAV LRCIYAYFMN VPQEESPWMS IPLHTLIKLE
410 420 430 440 450
PRAYGTKVTK IKANIPAVST YKEKGTSQVG ELSQSSTKLH QLLNDSPLED

KF
Length:452
Mass (Da):52,595
Last modified:November 1, 1995 - v2
Checksum:i228766A302F32F82
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti199E → Q in AAB22823 (PubMed:1322693).Curated1
Sequence conflicti362V → E in AAB22823 (PubMed:1322693).Curated1
Sequence conflicti448 – 449LE → FQ in AAB22823 (PubMed:1322693).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S42124 Genomic DNA Translation: AAB22823.1
Z49430 Genomic DNA Translation: CAA89450.1
BK006943 Genomic DNA Translation: DAA08648.1
PIRiS56938
RefSeqiNP_012380.1, NM_001181588.1

Genome annotation databases

EnsemblFungiiYJL155C; YJL155C; YJL155C
GeneIDi853286
KEGGisce:YJL155C

Similar proteinsi

Entry informationi

Entry nameiF26_YEAST
AccessioniPrimary (citable) accession number: P32604
Secondary accession number(s): D6VW32
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 172 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

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