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P32603

- SPR1_YEAST

UniProt

P32603 - SPR1_YEAST

Protein

Sporulation-specific glucan 1,3-beta-glucosidase

Gene

SPR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Probably involved in the processes of spore formation and contributes to ascospore thermoresistance by participating in the morphogenesis of ascospore walls. The enzyme may do this by modifying glucan linkages in the developing ascospore wall, thus strengthening it or lending it plasticity.

    Catalytic activityi

    Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei233 – 2331Proton donorBy similarity
    Active sitei335 – 3351NucleophileBy similarity

    GO - Molecular functioni

    1. glucan exo-1,3-beta-glucosidase activity Source: SGD

    GO - Biological processi

    1. ascospore formation Source: SGD
    2. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation, Sporulation

    Enzyme and pathway databases

    BioCyciYEAST:YOR190W-MONOMER.

    Protein family/group databases

    CAZyiGH5. Glycoside Hydrolase Family 5.
    mycoCLAPiEXG5C_YEAST.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sporulation-specific glucan 1,3-beta-glucosidase (EC:3.2.1.58)
    Alternative name(s):
    Exo-1,3-beta-glucanase
    Gene namesi
    Name:SPR1
    Synonyms:SSG1
    Ordered Locus Names:YOR190W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR190w.
    SGDiS000005716. SPR1.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. ascospore wall Source: SGD
    2. extracellular region Source: UniProtKB-SubCell
    3. fungal-type cell wall Source: SGD

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 445424Sporulation-specific glucan 1,3-beta-glucosidasePRO_0000007898Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi201 – 2011N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Developmental stagei

    Late stages of sporulation, during ascospore wall formation. Probably functions before partitioning of nuclei or is distributed to developing ascospores prior to the completion of pro-spore walls.

    Gene expression databases

    GenevestigatoriP32603.

    Interactioni

    Protein-protein interaction databases

    BioGridi34585. 5 interactions.
    DIPiDIP-3910N.
    MINTiMINT-487637.
    STRINGi4932.YOR190W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32603.
    SMRiP32603. Positions 42-444.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2730.
    GeneTreeiENSGT00390000009809.
    HOGENOMiHOG000114462.
    KOiK01210.
    OMAiIANTRRF.
    OrthoDBiEOG7JT75H.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00150. Cellulase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32603-1 [UniParc]FASTAAdd to Basket

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    MVSFRGLTTL TLLFTKLVNC NPVSTKNRDS IQFIYKEKDS IYSAINNQAI    50
    NEKIHGVNLG GWLVLEPYIT PSLFETFRTN PYNDDGIPVD EYHFCEKLGY 100
    EKAKERLYSH WSTFYKEEDF AKIASQGFNL VRIPIGYWAF TTLSHDPYVT 150
    AEQEYFLDRA IDWARKYGLK VWIDLHGAAG SQNGFDNSGL RDSYKFLEDE 200
    NLSATMKALT YILSKYSTDV YLDTVIGIEL LNEPLGPVID MERLKNLLLK 250
    PAYDYLRNKI NSNQIIVIHD AFQPYHYWDG FLNDEKNEYG VIIDHHHYQV 300
    FSQVELTRKM NERIKIACQW GKDAVSEKHW SVAGEFSAAL TDCTKWLNGV 350
    GLGARYDGSW TKDNEKSHYI NTCANNENIA LWPEERKQNT RKFIEAQLDA 400
    FEMTGGWIMW CYKTENSIEW DVEKLIQLNI FPQPINDRKY PNQCH 445
    Length:445
    Mass (Da):51,809
    Last modified:October 1, 1993 - v1
    Checksum:i0948A6E4703D41D4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S52935 Genomic DNA. Translation: AAB24895.1.
    X59259 Genomic DNA. Translation: CAA41952.1.
    Z75098 Genomic DNA. Translation: CAA99399.1.
    AY723868 Genomic DNA. Translation: AAU09785.1.
    BK006948 Genomic DNA. Translation: DAA10962.1.
    PIRiA40639.
    RefSeqiNP_014833.3. NM_001183609.3.

    Genome annotation databases

    EnsemblFungiiYOR190W; YOR190W; YOR190W.
    GeneIDi854362.
    KEGGisce:YOR190W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S52935 Genomic DNA. Translation: AAB24895.1 .
    X59259 Genomic DNA. Translation: CAA41952.1 .
    Z75098 Genomic DNA. Translation: CAA99399.1 .
    AY723868 Genomic DNA. Translation: AAU09785.1 .
    BK006948 Genomic DNA. Translation: DAA10962.1 .
    PIRi A40639.
    RefSeqi NP_014833.3. NM_001183609.3.

    3D structure databases

    ProteinModelPortali P32603.
    SMRi P32603. Positions 42-444.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34585. 5 interactions.
    DIPi DIP-3910N.
    MINTi MINT-487637.
    STRINGi 4932.YOR190W.

    Protein family/group databases

    CAZyi GH5. Glycoside Hydrolase Family 5.
    mycoCLAPi EXG5C_YEAST.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR190W ; YOR190W ; YOR190W .
    GeneIDi 854362.
    KEGGi sce:YOR190W.

    Organism-specific databases

    CYGDi YOR190w.
    SGDi S000005716. SPR1.

    Phylogenomic databases

    eggNOGi COG2730.
    GeneTreei ENSGT00390000009809.
    HOGENOMi HOG000114462.
    KOi K01210.
    OMAi IANTRRF.
    OrthoDBi EOG7JT75H.

    Enzyme and pathway databases

    BioCyci YEAST:YOR190W-MONOMER.

    Miscellaneous databases

    NextBioi 976473.

    Gene expression databases

    Genevestigatori P32603.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00150. Cellulase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Saccharomyces cerevisiae SPR1 gene encodes a sporulation-specific exo-1,3-beta-glucanase which contributes to ascospore thermoresistance."
      Muthukumar G., Suhng S.-H., Magee P.T., Jewell R.D., Primerano D.A.
      J. Bacteriol. 175:386-394(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "SSG1, a gene encoding a sporulation-specific 1,3-beta-glucanase in Saccharomyces cerevisiae."
      San Segundo P., Correa J., Vazquez de Aldana C.R., del Rey F.
      J. Bacteriol. 175:3823-3837(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / GRF88.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.

    Entry informationi

    Entry nameiSPR1_YEAST
    AccessioniPrimary (citable) accession number: P32603
    Secondary accession number(s): D6W2P6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3