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Protein

Alpha-soluble NSF attachment protein

Gene

SEC17

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

SNARE complex protein that binds to cis-SNARE complexes on membranes and is required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus and for homotypic vacuole fusion. During the priming step of membrane fusion, is released from cis-SNARE complexes by SEC18 to establish a pool of unpaired SNAREs, which are required for interactions in trans during docking and fusion steps. Can displace HOPS from SNARE complexes, which may be a prerequisite for trans-SNARE complex disassembly and subsequent rounds of priming, docking and fusion.4 Publications

GO - Molecular functioni

  • ATPase activator activity Source: SGD
  • soluble NSF attachment protein activity Source: SGD
  • syntaxin binding Source: GO_Central

GO - Biological processi

  • autophagy Source: SGD
  • intracellular protein transport Source: GO_Central
  • SNARE complex disassembly Source: SGD
  • vacuole fusion, non-autophagic Source: SGD
  • vesicle fusion with Golgi apparatus Source: SGD

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-28949-MONOMER
ReactomeiR-SCE-6807878 COPI-mediated anterograde transport
R-SCE-6811438 Intra-Golgi traffic

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-soluble NSF attachment protein
Short name:
SNAP-alpha
Alternative name(s):
N-ethylmaleimide-sensitive factor attachment protein alpha
Vesicular-fusion protein SEC17
alpha-SNAP chaperone
Gene namesi
Name:SEC17
Ordered Locus Names:YBL050W
ORF Names:YBL0505, YBL0517
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL050W
SGDiS000000146 SEC17

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00002190762 – 292Alpha-soluble NSF attachment proteinAdd BLAST291

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1 Publication1
Cross-linki261Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP32602
PaxDbiP32602
PRIDEiP32602

PTM databases

iPTMnetiP32602

Interactioni

Subunit structurei

Binds to vacuolar cis-SNARE complexes composed of the v-SNAREs NYV1, VTI1 and YKT6, and the t-SNAREs VAM3 and VAM7. Interacts with SEC18.1 Publication

GO - Molecular functioni

  • soluble NSF attachment protein activity Source: SGD
  • syntaxin binding Source: GO_Central

Protein-protein interaction databases

BioGridi32648, 390 interactors
DIPiDIP-2496N
IntActiP32602, 36 interactors
MINTiP32602
STRINGi4932.YBL050W

Structurei

Secondary structure

1292
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 14Combined sources11
Helixi21 – 25Combined sources5
Helixi30 – 49Combined sources20
Helixi54 – 69Combined sources16
Helixi73 – 89Combined sources17
Helixi93 – 109Combined sources17
Helixi113 – 129Combined sources17
Helixi134 – 150Combined sources17
Helixi154 – 170Combined sources17
Helixi174 – 186Combined sources13
Turni192 – 194Combined sources3
Helixi195 – 197Combined sources3
Helixi198 – 211Combined sources14
Helixi215 – 223Combined sources9
Helixi224 – 226Combined sources3
Helixi238 – 249Combined sources12
Turni253 – 255Combined sources3
Helixi256 – 263Combined sources8
Helixi271 – 288Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QQEX-ray2.90A1-292[»]
ProteinModelPortaliP32602
SMRiP32602
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32602

Family & Domainsi

Sequence similaritiesi

Belongs to the SNAP family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000005826
HOGENOMiHOG000165015
KOiK15296
OMAiMADNEQK
OrthoDBiEOG092C4020

Family and domain databases

CDDicd15832 SNAP, 1 hit
Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR000744 NSF_attach
IPR011990 TPR-like_helical_dom_sf
PANTHERiPTHR13768 PTHR13768, 1 hit
PRINTSiPR00448 NSFATTACHMNT
SUPFAMiSSF48452 SSF48452, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32602-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDPVELLKR AEKKGVPSSG FMKLFSGSDS YKFEEAADLC VQAATIYRLR
60 70 80 90 100
KELNLAGDSF LKAADYQKKA GNEDEAGNTY VEAYKCFKSG GNSVNAVDSL
110 120 130 140 150
ENAIQIFTHR GQFRRGANFK FELGEILEND LHDYAKAIDC YELAGEWYAQ
160 170 180 190 200
DQSVALSNKC FIKCADLKAL DGQYIEASDI YSKLIKSSMG NRLSQWSLKD
210 220 230 240 250
YFLKKGLCQL AATDAVAAAR TLQEGQSEDP NFADSRESNF LKSLIDAVNE
260 270 280 290
GDSEQLSEHC KEFDNFMRLD KWKITILNKI KESIQQQEDD LL
Length:292
Mass (Da):32,803
Last modified:January 23, 2007 - v4
Checksum:i5002D31F80A65319
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti103A → P in AAA35029 (PubMed:1601878).Curated1
Sequence conflicti219Missing in AAA35029 (PubMed:1601878).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93104 Genomic DNA Translation: AAA35029.1
Z23261 Genomic DNA Translation: CAA80796.1
Z35811 Genomic DNA Translation: CAA84870.1
BK006936 Genomic DNA Translation: DAA07069.1
PIRiS39837
RefSeqiNP_009503.1, NM_001178290.1

Genome annotation databases

EnsemblFungiiYBL050W; YBL050W; YBL050W
GeneIDi852230
KEGGisce:YBL050W

Similar proteinsi

Entry informationi

Entry nameiSEC17_YEAST
AccessioniPrimary (citable) accession number: P32602
Secondary accession number(s): D6VPU9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 164 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health