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Protein

Alpha-soluble NSF attachment protein

Gene

SEC17

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SNARE complex protein that binds to cis-SNARE complexes on membranes and is required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus and for homotypic vacuole fusion. During the priming step of membrane fusion, is released from cis-SNARE complexes by SEC18 to establish a pool of unpaired SNAREs, which are required for interactions in trans during docking and fusion steps. Can displace HOPS from SNARE complexes, which may be a prerequisite for trans-SNARE complex disassembly and subsequent rounds of priming, docking and fusion.4 Publications

GO - Molecular functioni

  • ATPase activator activity Source: SGD
  • soluble NSF attachment protein activity Source: SGD

GO - Biological processi

  • autophagy Source: SGD
  • intracellular protein transport Source: InterPro
  • SNARE complex disassembly Source: SGD
  • vacuole fusion, non-autophagic Source: SGD
  • vesicle fusion with Golgi apparatus Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-28949-MONOMER.
ReactomeiR-SCE-6807878. COPI-mediated anterograde transport.
R-SCE-6811438. Intra-Golgi traffic.
R-SCE-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-soluble NSF attachment protein
Short name:
SNAP-alpha
Alternative name(s):
N-ethylmaleimide-sensitive factor attachment protein alpha
Vesicular-fusion protein SEC17
alpha-SNAP chaperone
Gene namesi
Name:SEC17
Ordered Locus Names:YBL050W
ORF Names:YBL0505, YBL0517
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL050W.
SGDiS000000146. SEC17.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • extrinsic component of membrane Source: SGD
  • SNARE complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources1 Publication
Chaini2 – 292291Alpha-soluble NSF attachment proteinPRO_0000219076Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources1 Publication
Cross-linki261 – 261Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP32602.

Interactioni

Subunit structurei

Binds to vacuolar cis-SNARE complexes composed of the v-SNAREs NYV1, VTI1 and YKT6, and the t-SNAREs VAM3 and VAM7. Interacts with SEC18.1 Publication

GO - Molecular functioni

  • soluble NSF attachment protein activity Source: SGD

Protein-protein interaction databases

BioGridi32648. 137 interactions.
DIPiDIP-2496N.
IntActiP32602. 35 interactions.
MINTiMINT-491467.

Structurei

Secondary structure

1
292
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1411Combined sources
Helixi21 – 255Combined sources
Helixi30 – 4920Combined sources
Helixi54 – 6916Combined sources
Helixi73 – 8917Combined sources
Helixi93 – 10917Combined sources
Helixi113 – 12917Combined sources
Helixi134 – 15017Combined sources
Helixi154 – 17017Combined sources
Helixi174 – 18613Combined sources
Turni192 – 1943Combined sources
Helixi195 – 1973Combined sources
Helixi198 – 21114Combined sources
Helixi215 – 2239Combined sources
Helixi224 – 2263Combined sources
Helixi238 – 24912Combined sources
Turni253 – 2553Combined sources
Helixi256 – 2638Combined sources
Helixi271 – 28818Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QQEX-ray2.90A1-292[»]
ProteinModelPortaliP32602.
SMRiP32602. Positions 1-290.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32602.

Family & Domainsi

Sequence similaritiesi

Belongs to the SNAP family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000005826.
HOGENOMiHOG000165015.
KOiK15296.
OMAiINCLNRA.
OrthoDBiEOG092C4020.

Family and domain databases

CDDicd15832. SNAP. 1 hit.
Gene3Di1.25.40.10. 1 hit.
InterProiIPR000744. NSF_attach.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR13768. PTHR13768. 1 hit.
PRINTSiPR00448. NSFATTACHMNT.
SUPFAMiSSF48452. SSF48452. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32602-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDPVELLKR AEKKGVPSSG FMKLFSGSDS YKFEEAADLC VQAATIYRLR
60 70 80 90 100
KELNLAGDSF LKAADYQKKA GNEDEAGNTY VEAYKCFKSG GNSVNAVDSL
110 120 130 140 150
ENAIQIFTHR GQFRRGANFK FELGEILEND LHDYAKAIDC YELAGEWYAQ
160 170 180 190 200
DQSVALSNKC FIKCADLKAL DGQYIEASDI YSKLIKSSMG NRLSQWSLKD
210 220 230 240 250
YFLKKGLCQL AATDAVAAAR TLQEGQSEDP NFADSRESNF LKSLIDAVNE
260 270 280 290
GDSEQLSEHC KEFDNFMRLD KWKITILNKI KESIQQQEDD LL
Length:292
Mass (Da):32,803
Last modified:January 23, 2007 - v4
Checksum:i5002D31F80A65319
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti103 – 1031A → P in AAA35029 (PubMed:1601878).Curated
Sequence conflicti219 – 2191Missing in AAA35029 (PubMed:1601878).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93104 Genomic DNA. Translation: AAA35029.1.
Z23261 Genomic DNA. Translation: CAA80796.1.
Z35811 Genomic DNA. Translation: CAA84870.1.
BK006936 Genomic DNA. Translation: DAA07069.1.
PIRiS39837.
RefSeqiNP_009503.1. NM_001178290.1.

Genome annotation databases

EnsemblFungiiYBL050W; YBL050W; YBL050W.
GeneIDi852230.
KEGGisce:YBL050W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93104 Genomic DNA. Translation: AAA35029.1.
Z23261 Genomic DNA. Translation: CAA80796.1.
Z35811 Genomic DNA. Translation: CAA84870.1.
BK006936 Genomic DNA. Translation: DAA07069.1.
PIRiS39837.
RefSeqiNP_009503.1. NM_001178290.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QQEX-ray2.90A1-292[»]
ProteinModelPortaliP32602.
SMRiP32602. Positions 1-290.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32648. 137 interactions.
DIPiDIP-2496N.
IntActiP32602. 35 interactions.
MINTiMINT-491467.

Proteomic databases

MaxQBiP32602.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL050W; YBL050W; YBL050W.
GeneIDi852230.
KEGGisce:YBL050W.

Organism-specific databases

EuPathDBiFungiDB:YBL050W.
SGDiS000000146. SEC17.

Phylogenomic databases

GeneTreeiENSGT00390000005826.
HOGENOMiHOG000165015.
KOiK15296.
OMAiINCLNRA.
OrthoDBiEOG092C4020.

Enzyme and pathway databases

BioCyciYEAST:G3O-28949-MONOMER.
ReactomeiR-SCE-6807878. COPI-mediated anterograde transport.
R-SCE-6811438. Intra-Golgi traffic.
R-SCE-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

EvolutionaryTraceiP32602.
PROiP32602.

Family and domain databases

CDDicd15832. SNAP. 1 hit.
Gene3Di1.25.40.10. 1 hit.
InterProiIPR000744. NSF_attach.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PANTHERiPTHR13768. PTHR13768. 1 hit.
PRINTSiPR00448. NSFATTACHMNT.
SUPFAMiSSF48452. SSF48452. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSEC17_YEAST
AccessioniPrimary (citable) accession number: P32602
Secondary accession number(s): D6VPU9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 151 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.