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P32594

- GAG_MSVMT

UniProt

P32594 - GAG_MSVMT

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Protein

Gag polyprotein

Gene

gag

Organism
Moloney murine sarcoma virus (strain ts110) (MoMSV)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release (By similarity).By similarity
Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex (By similarity).By similarity
Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.By similarity
Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei131 – 1322Cleavage; by viral proteaseBy similarity
Sitei215 – 2162Cleavage; by viral proteaseBy similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural constituent of virion Source: UniProtKB-KW

GO - Biological processi

  1. viral budding via host ESCRT complex Source: UniProtKB-KW
  2. viral release from host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Virus exit from host cell

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 3 chains:
Matrix protein p15
Short name:
MA
Alternative name(s):
pp12
Capsid protein p30
Short name:
CA
Gene namesi
Name:gag
OrganismiMoloney murine sarcoma virus (strain ts110) (MoMSV)
Taxonomic identifieri31691 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirus
Virus hostiMus musculus (Mouse) [TaxID: 10090]

Subcellular locationi

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
  3. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by hostBy similarity
Chaini2 – 468467Gag polyproteinPRO_0000390819Add
BLAST
Chaini2 – 131130Matrix protein p15Sequence AnalysisPRO_0000040953Add
BLAST
Chaini132 – 21584RNA-binding phosphoprotein p12Sequence AnalysisPRO_0000040954Add
BLAST
Chaini216 – 468253Capsid protein p30Sequence AnalysisPRO_0000040955Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine; by hostBy similarity

Post-translational modificationi

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Myristate

Interactioni

Subunit structurei

Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP32594.
SMRiP32594. Positions 2-98, 216-346, 352-382.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi111 – 1144PTAP/PSAP motif
Motifi130 – 1345LYPX(n)L motif
Motifi162 – 1654PPXY motif

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 also contains one L domain: a LYPX(n)L which potentially interacts with PDCD6IP (By similarity).By similarity

Family and domain databases

Gene3Di1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
InterProiIPR000840. G_retro_matrix_N.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
[Graphical view]
PfamiPF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
[Graphical view]
SUPFAMiSSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32594 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MGQTVTTPLS LTLDHWKDVE RIAHNQSVDV KKRRWVTFCS AEWPTFNVGW
60 70 80 90 100
PRDGTFNRDL ITQVKIKVFS PGPHGHPDQV PYIVTWEALA FDPPPWVKPF
110 120 130 140 150
VHPKPPPPLL PSAPSLPLEP PLSTPPQSSL YPALTPSLGA KPKPQVLSDS
160 170 180 190 200
GGPLIDLLTE DPPPYRDPRP PPSDRDGDSG EATPAGEAPD PSPMASRLRG
210 220 230 240 250
RREPPVADST TSQAFPLRTG GNGQLQYWPF SSSDLYNWKS NNPSFSEDPG
260 270 280 290 300
KLTALIESVL ITHQPTWDDC QQLLGTLLTG EEKQRVLLEA RKAVRGDDGR
310 320 330 340 350
PTQLPNEVDA AFPLERPDWE YTTQAGRNHL VHYRQLLIAG LQNAGRSPTN
360 370 380 390 400
LAKVKGITQG PNESPSAFLE RLKEAYRRYT PYDPEDPGQE TNVSMSFIWQ
410 420 430 440 450
SAPDIGRKLE RLEDLRNKTL GDLVREAERI FNKRETPEER EERIRREREE
460
KEERHAPKLP WLFLIISP
Length:468
Mass (Da):52,681
Last modified:January 23, 2007 - v3
Checksum:iAF4F795F5D69824F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M96854 Genomic DNA. No translation available.
PIRiA42745. FOMVMU.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M96854 Genomic DNA. No translation available.
PIRi A42745. FOMVMU.

3D structure databases

ProteinModelPortali P32594.
SMRi P32594. Positions 2-98, 216-346, 352-382.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
InterProi IPR000840. G_retro_matrix_N.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
[Graphical view ]
Pfami PF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
[Graphical view ]
SUPFAMi SSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Moloney murine sarcoma virus MuSVts110 DNA: cloning, nucleotide sequence, and gene expression."
    Huai L., Chiocca S.M., Gilbreth M.A., Ainsworth J.R., Bishop L.A., Murphy E.C. Jr.
    J. Virol. 66:5329-5337(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGAG_MSVMT
AccessioniPrimary (citable) accession number: P32594
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 80 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is probably translated as a Gag-Mos polyprotein.

External Data

Dasty 3