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Protein

Integrin beta-2

Gene

ITGB2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. Integrin alpha-L/beta-2 in assosiation with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi138 – 1381Calcium; via carbonyl oxygenBy similarity
Metal bindingi141 – 1411CalciumBy similarity
Metal bindingi142 – 1421CalciumBy similarity
Metal bindingi347 – 3471CalciumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Integrin, Receptor

Keywords - Biological processi

Cell adhesion, Phagocytosis

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-166016. Toll Like Receptor 4 (TLR4) Cascade.
R-BTA-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-BTA-202733. Cell surface interactions at the vascular wall.
R-BTA-216083. Integrin cell surface interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin beta-2
Alternative name(s):
Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta
Complement receptor C3 subunit beta
CD_antigen: CD18
Gene namesi
Name:ITGB2
Synonyms:CD18
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 1

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 700678ExtracellularSequence analysisAdd
BLAST
Transmembranei701 – 72323HelicalSequence analysisAdd
BLAST
Topological domaini724 – 76946CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in ITGB2 are the cause of leukocyte adhesion deficiency (LAD). The mutation causing LAD (Gly-128) is prevalent among Holstein cattle throughout the world, placing this disorder among the most common genetic diseases known in animal agriculture. All cattle with the mutant allele are related to one bull, who through the use of artificial insemination sired many calves in the 1950s and 1960s.

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222By similarityAdd
BLAST
Chaini23 – 769747Integrin beta-2PRO_0000016340Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi25 ↔ 43By similarity
Disulfide bondi33 ↔ 447By similarity
Disulfide bondi36 ↔ 62By similarity
Disulfide bondi46 ↔ 73By similarity
Glycosylationi50 – 501N-linked (GlcNAc...)Sequence analysis
Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence analysis
Disulfide bondi191 ↔ 198By similarity
Disulfide bondi246 ↔ 286By similarity
Glycosylationi254 – 2541N-linked (GlcNAc...)Sequence analysis
Disulfide bondi386 ↔ 400By similarity
Disulfide bondi420 ↔ 445By similarity
Disulfide bondi449 ↔ 467By similarity
Disulfide bondi459 ↔ 470By similarity
Disulfide bondi472 ↔ 481By similarity
Disulfide bondi483 ↔ 514By similarity
Disulfide bondi497 ↔ 512By similarity
Glycosylationi501 – 5011N-linked (GlcNAc...)Sequence analysis
Disulfide bondi506 ↔ 517By similarity
Disulfide bondi519 ↔ 534By similarity
Disulfide bondi536 ↔ 559By similarity
Disulfide bondi541 ↔ 557By similarity
Disulfide bondi549 ↔ 562By similarity
Disulfide bondi564 ↔ 573By similarity
Disulfide bondi575 ↔ 598By similarity
Disulfide bondi582 ↔ 596By similarity
Disulfide bondi590 ↔ 601By similarity
Disulfide bondi603 ↔ 612By similarity
Disulfide bondi615 ↔ 618By similarity
Disulfide bondi622 ↔ 662By similarity
Disulfide bondi628 ↔ 647By similarity
Disulfide bondi631 ↔ 643By similarity
Glycosylationi642 – 6421N-linked (GlcNAc...)Sequence analysis
Disulfide bondi670 ↔ 695By similarity
Modified residuei745 – 7451PhosphoserineBy similarity
Modified residuei756 – 7561PhosphoserineBy similarity
Modified residuei758 – 7581PhosphothreonineBy similarity
Modified residuei760 – 7601PhosphothreonineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP32592.
PeptideAtlasiP32592.
PRIDEiP32592.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Beta-2 associates with either alpha-L, alpha-M, alpha-X or alpha-D. Interacts with COPS5 and RANBP9. Interacts with FGR. Interacts with FLNA (via filamin repeats 4, 9, 12, 17, 19, 21, and 23). Interacts with THBD.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
lktAQ7BHI83EBI-11509482,EBI-11580242From a different organism.

Protein-protein interaction databases

IntActiP32592. 1 interaction.
STRINGi9913.ENSBTAP00000022687.

Structurei

3D structure databases

ProteinModelPortaliP32592.
SMRiP32592. Positions 23-574.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini124 – 363240VWFAAdd
BLAST
Repeati449 – 49648IAdd
BLAST
Repeati497 – 54044IIAdd
BLAST
Repeati541 – 58141IIIAdd
BLAST
Repeati582 – 61736IVAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni449 – 617169Cysteine-rich tandem repeatsAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi397 – 3993Cell attachment siteSequence analysis

Sequence similaritiesi

Belongs to the integrin beta chain family.Curated
Contains 1 VWFA domain.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1226. Eukaryota.
ENOG410XP60. LUCA.
GeneTreeiENSGT00760000119064.
HOGENOMiHOG000252936.
HOVERGENiHBG006190.
InParanoidiP32592.
KOiK06464.
OMAiYISCAEC.
OrthoDBiEOG7T7GSB.
TreeFamiTF105392.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR015812. Integrin_bsu.
IPR015439. Integrin_bsu-2.
IPR014836. Integrin_bsu_cyt_dom.
IPR002369. Integrin_bsu_N.
IPR012896. Integrin_bsu_tail.
IPR032695. Integrin_dom.
IPR016201. Plexin-like_fold.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10082. PTHR10082. 1 hit.
PTHR10082:SF15. PTHR10082:SF15. 1 hit.
PfamiPF08725. Integrin_b_cyt. 1 hit.
PF07965. Integrin_B_tail. 1 hit.
PF00362. Integrin_beta. 1 hit.
[Graphical view]
PIRSFiPIRSF002512. Integrin_B. 1 hit.
PRINTSiPR01186. INTEGRINB.
SMARTiSM00187. INB. 1 hit.
SM01241. Integrin_b_cyt. 1 hit.
SM01242. Integrin_B_tail. 1 hit.
SM00423. PSI. 1 hit.
[Graphical view]
SUPFAMiSSF103575. SSF103575. 1 hit.
SSF53300. SSF53300. 1 hit.
SSF69179. SSF69179. 1 hit.
SSF69687. SSF69687. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 2 hits.
PS00243. INTEGRIN_BETA. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32592-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRQRPQLLL LAGLLALQSV LSQECTNYKV STCRDCIESG PGCAWCQKLN
60 70 80 90 100
FTGQGEPDSI RCDTRAELLS KGCPADDIME PKSLAETRDS QAGSRKQLSP
110 120 130 140 150
QEVTLYLRPG QAVAFNVTFR RAKGYPIDLY YLMDLSYSMV DDLVNVKKLG
160 170 180 190 200
GDLLRALNGI TESGRIGFGS FVDKTVLPFV NTHPEKLRNP CPNKEKECQP
210 220 230 240 250
PFAFRHVLKL TDNSKQFETE VGKQLISGNL DAPEGGLDAM MQVAACPEEI
260 270 280 290 300
GWRNVTRLLV FATDDGFHFA GDGKLGAILT PNDGRCHLED NLYKSSNEFD
310 320 330 340 350
YPSVGQLAHK LAESNIQPIF AVTKKMVKTY EKLTEIIPKS AVGELSEDSR
360 370 380 390 400
NVVELIKNAY NKLSSRVFLD HSTLPDTLKV TYDSFCSNGK SQVDQPRGDC
410 420 430 440 450
DGVQINVPIT FQVKVTATEC IQQQSFTIRA LGFTDTVTVR VLPQCECQCR
460 470 480 490 500
DASRDGSICG GRGSMECGVC RCDAGYIGKN CECQTQGRSS QELEGSCRKD
510 520 530 540 550
NSSIICSGLG DCICGQCVCH TSDVPNKKIY GQFCECDNVN CERYDGQVCG
560 570 580 590 600
GEKRGLCFCG TCRCDEQYEG SACQCLKSTQ GCLNLDGVEC SGRGRCRCNV
610 620 630 640 650
CQCDPGYQPP LCSECPGCPV PCAGFAPCTE CLKFDKGPFA KNCSAACGQT
660 670 680 690 700
KLLSSPVPGR KCKERDSEGC WMTYTLVQRD GRDRYDVHVD DMLECVKGPN
710 720 730 740 750
IAAIVGGTVG GVVLVGILLL VIWKALTHLS DLREYHRFEK EKLKSQWNND
760
NPLFKSATTT VMNPKFAES
Length:769
Mass (Da):84,400
Last modified:October 1, 1993 - v1
Checksum:i5903ADF4E8998CEA
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti128 – 1281D → G in LAD. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81233 mRNA. Translation: AAA30438.1.
PIRiJC1121.
RefSeqiNP_786975.1. NM_175781.1.
XP_015329203.1. XM_015473717.1.
UniGeneiBt.63143.

Genome annotation databases

EnsembliENSBTAT00000022687; ENSBTAP00000022687; ENSBTAG00000017060.
GeneIDi281877.
KEGGibta:281877.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81233 mRNA. Translation: AAA30438.1.
PIRiJC1121.
RefSeqiNP_786975.1. NM_175781.1.
XP_015329203.1. XM_015473717.1.
UniGeneiBt.63143.

3D structure databases

ProteinModelPortaliP32592.
SMRiP32592. Positions 23-574.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP32592. 1 interaction.
STRINGi9913.ENSBTAP00000022687.

Proteomic databases

PaxDbiP32592.
PeptideAtlasiP32592.
PRIDEiP32592.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000022687; ENSBTAP00000022687; ENSBTAG00000017060.
GeneIDi281877.
KEGGibta:281877.

Organism-specific databases

CTDi3689.

Phylogenomic databases

eggNOGiKOG1226. Eukaryota.
ENOG410XP60. LUCA.
GeneTreeiENSGT00760000119064.
HOGENOMiHOG000252936.
HOVERGENiHBG006190.
InParanoidiP32592.
KOiK06464.
OMAiYISCAEC.
OrthoDBiEOG7T7GSB.
TreeFamiTF105392.

Enzyme and pathway databases

ReactomeiR-BTA-166016. Toll Like Receptor 4 (TLR4) Cascade.
R-BTA-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-BTA-202733. Cell surface interactions at the vascular wall.
R-BTA-216083. Integrin cell surface interactions.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR015812. Integrin_bsu.
IPR015439. Integrin_bsu-2.
IPR014836. Integrin_bsu_cyt_dom.
IPR002369. Integrin_bsu_N.
IPR012896. Integrin_bsu_tail.
IPR032695. Integrin_dom.
IPR016201. Plexin-like_fold.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10082. PTHR10082. 1 hit.
PTHR10082:SF15. PTHR10082:SF15. 1 hit.
PfamiPF08725. Integrin_b_cyt. 1 hit.
PF07965. Integrin_B_tail. 1 hit.
PF00362. Integrin_beta. 1 hit.
[Graphical view]
PIRSFiPIRSF002512. Integrin_B. 1 hit.
PRINTSiPR01186. INTEGRINB.
SMARTiSM00187. INB. 1 hit.
SM01241. Integrin_b_cyt. 1 hit.
SM01242. Integrin_B_tail. 1 hit.
SM00423. PSI. 1 hit.
[Graphical view]
SUPFAMiSSF103575. SSF103575. 1 hit.
SSF53300. SSF53300. 1 hit.
SSF69179. SSF69179. 1 hit.
SSF69687. SSF69687. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 2 hits.
PS00243. INTEGRIN_BETA. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of the bovine CD18-encoding cDNA: comparison with the human and murine glycoproteins."
    Shuster D.E., Bosworth B.T., Kehrli M.E. Jr.
    Gene 114:267-271(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Identification and prevalence of a genetic defect that causes leukocyte adhesion deficiency in Holstein cattle."
    Shuster D.E., Kehrli M.E. Jr., Ackermann M.R., Gilbert R.O.
    Proc. Natl. Acad. Sci. U.S.A. 89:9225-9229(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT LAD GLY-128, DISEASE.

Entry informationi

Entry nameiITB2_BOVIN
AccessioniPrimary (citable) accession number: P32592
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 6, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.