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Protein

SWI/SNF complex subunit SWI3

Gene

SWI3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in transcriptional activation. Component of the SWI/SNF complex, an ATP-dependent chromatin-remodeling complex, which is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors.

GO - Molecular functioni

  • DNA binding Source: SGD
  • histone binding Source: SGD

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: SGD
  • carbon catabolite activation of transcription from RNA polymerase II promoter Source: SGD
  • cellular alcohol catabolic process Source: SGD
  • positive regulation of mating type switching Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • sucrose catabolic process Source: SGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31611-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
SWI/SNF complex subunit SWI3
Alternative name(s):
Transcription factor TYE2
Transcription regulatory protein SWI3
Gene namesi
Name:SWI3
Synonyms:TYE2
Ordered Locus Names:YJL176C
ORF Names:J0495
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL176C.
SGDiS000003712. SWI3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: SGD
  • SWI/SNF complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi374 – 3741D → A: Loss of DNA-binding. 1 Publication
Mutagenesisi383 – 3831K → D: Loss of DNA-binding; when associated with D-387. 1 Publication
Mutagenesisi387 – 3871K → D: Loss of DNA-binding; when associated with D-383. 1 Publication
Mutagenesisi392 – 3921N → A: Loss of DNA-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 825825SWI/SNF complex subunit SWI3PRO_0000197123Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei88 – 881PhosphoserineCombined sources
Modified residuei185 – 1851PhosphoserineCombined sources
Modified residuei235 – 2351PhosphothreonineCombined sources
Modified residuei657 – 6571PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32591.
PeptideAtlasiP32591.

PTM databases

iPTMnetiP32591.

Interactioni

Subunit structurei

Interacts with RTT102, SWP82 and the N-terminus of SNF2. Component of the SWI/SNF global transcription activator complex. The 1.14 MDa SWI/SNF complex is composed of 11 different subunits: one copy each of SWI1, SNF2/SWI2, SNF5, SNF12/SWP73, ARP7/SWP61, ARP9/SWP59; two copies each of SWI3, SNF6, SNF11, SWP82; and three copies of TAF14/SWP29.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RTT102P533302EBI-18622,EBI-23637
SNF2P220826EBI-18622,EBI-17526
SNF6P188885EBI-18622,EBI-17550

GO - Molecular functioni

  • histone binding Source: SGD

Protein-protein interaction databases

BioGridi33585. 288 interactions.
DIPiDIP-2471N.
IntActiP32591. 48 interactions.
MINTiMINT-563159.

Structurei

Secondary structure

1
825
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi321 – 3266Combined sources
Helixi328 – 3303Combined sources
Helixi340 – 35617Combined sources
Helixi364 – 3707Combined sources
Helixi375 – 38713Combined sources
Beta strandi390 – 3934Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FQ3X-ray1.40A309-398[»]
ProteinModelPortaliP32591.
SMRiP32591. Positions 311-395.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP32591.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini305 – 40298SWIRMPROSITE-ProRule annotationAdd
BLAST
Domaini522 – 57352SANTPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni694 – 72229Leucine-zipperAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 304304Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 1 SANT domain.PROSITE-ProRule annotation
Contains 1 SWIRM domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000018166.
HOGENOMiHOG000142068.
InParanoidiP32591.
KOiK11772.
OrthoDBiEOG7ZWDBR.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.10.60. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR032451. SMARCC_C.
IPR033051. Swi3.
IPR007526. SWIRM.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12802:SF53. PTHR12802:SF53. 1 hit.
PfamiPF00249. Myb_DNA-binding. 1 hit.
PF04433. SWIRM. 1 hit.
PF16495. SWIRM-assoc_1. 1 hit.
[Graphical view]
SMARTiSM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS51293. SANT. 1 hit.
PS50934. SWIRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32591-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENTLGEGST VNASVDVDQH GNDNNSDSNA NAAVAGVANT DTAGEESQQQ
60 70 80 90 100
DESLKDEATV PNTRDAESEA ITVTAKQQPT MQANKLDSQE TPSTEESRAQ
110 120 130 140 150
NVFGQDNEDS DNLFGETESS VSNNEANTPS IPTNPVDNEN NKPAIKEDST
160 170 180 190 200
IQDSNGDVKN MEDVKIQKEE EPENNTVIEG VKEESQPDEN TKEMDEVEED
210 220 230 240 250
DEDDDQPMIS PDNSIFGDTK SESKQLGNTS SVANTPSEIP DAHKAEQEDI
260 270 280 290 300
IEKTESVDKK VDSGEERNEQ EREIMNDHSK SANPKKTTIT RVEPETFEIP
310 320 330 340 350
QAHEIVIPSY SKWFNLEKIH SIEVQSLPEF FTNRIPSKTP EVYMRYRNFM
360 370 380 390 400
VNSYRLNPNE YFSVTTARRN VSGDAAALFR LHKFLTKWGL INYQVDSKLL
410 420 430 440 450
PKNIEPPLTS QYSTRHDAPR GLFPFESYKP SVQLPDMAKL KKMMNTSDSE
460 470 480 490 500
STLYKYLKES KRKYDEITHP PSTTDDENGD KNDNGGKMNN EVSTSTSMTG
510 520 530 540 550
DANLLEEGET SRPLKKVKIL EQIDENWSKE DLQKLLKGIQ EFGADWYKVA
560 570 580 590 600
KNVGNKSPEQ CILRFLQLPI EDKFLYGDGN GKGDNDNGLG PLKYAPHLPF
610 620 630 640 650
SKSENPVLST IAFLVGLVNP KTVQSMTQRA IQSAESIKSQ KEEISDQKPI
660 670 680 690 700
EHIKEGSEIA ISSLGYRSHI FATNEERQMN FLTNELIRLQ MEKLDAKLNH
710 720 730 740 750
LKKLEKFMEL ERKTLERQQE NLLIQRLNFN QNSSKIVNVL SKCLNLISDS
760 770 780 790 800
NINNSSVAEK EEIRSQIDHF KSMLSKPETL SIGKNPFNKP NIETGENHNG
810 820
QSISNENDVK PISIEAPQFY RYWSA
Length:825
Mass (Da):92,926
Last modified:October 1, 1993 - v1
Checksum:iDBA2E2022F15F9A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84390 Genomic DNA. Translation: AAA35136.1.
X56792 Genomic DNA. Translation: CAA40112.1.
Z49451 Genomic DNA. Translation: CAA89470.1.
BK006943 Genomic DNA. Translation: DAA08629.1.
PIRiS26706.
RefSeqiNP_012359.1. NM_001181609.1.

Genome annotation databases

EnsemblFungiiYJL176C; YJL176C; YJL176C.
GeneIDi853264.
KEGGisce:YJL176C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84390 Genomic DNA. Translation: AAA35136.1.
X56792 Genomic DNA. Translation: CAA40112.1.
Z49451 Genomic DNA. Translation: CAA89470.1.
BK006943 Genomic DNA. Translation: DAA08629.1.
PIRiS26706.
RefSeqiNP_012359.1. NM_001181609.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FQ3X-ray1.40A309-398[»]
ProteinModelPortaliP32591.
SMRiP32591. Positions 311-395.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33585. 288 interactions.
DIPiDIP-2471N.
IntActiP32591. 48 interactions.
MINTiMINT-563159.

PTM databases

iPTMnetiP32591.

Proteomic databases

MaxQBiP32591.
PeptideAtlasiP32591.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL176C; YJL176C; YJL176C.
GeneIDi853264.
KEGGisce:YJL176C.

Organism-specific databases

EuPathDBiFungiDB:YJL176C.
SGDiS000003712. SWI3.

Phylogenomic databases

GeneTreeiENSGT00390000018166.
HOGENOMiHOG000142068.
InParanoidiP32591.
KOiK11772.
OrthoDBiEOG7ZWDBR.

Enzyme and pathway databases

BioCyciYEAST:G3O-31611-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP32591.
PROiP32591.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.10.60. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR032451. SMARCC_C.
IPR033051. Swi3.
IPR007526. SWIRM.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12802:SF53. PTHR12802:SF53. 1 hit.
PfamiPF00249. Myb_DNA-binding. 1 hit.
PF04433. SWIRM. 1 hit.
PF16495. SWIRM-assoc_1. 1 hit.
[Graphical view]
SMARTiSM00717. SANT. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
PROSITEiPS51293. SANT. 1 hit.
PS50934. SWIRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the yeast SWI1, SWI2, and SWI3 genes, which encode a global activator of transcription."
    Peterson C.L., Herskowitz I.
    Cell 68:573-583(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Isolation of the TYE2 gene reveals its identity to SWI3 encoding a general transcription factor in Saccharomyces cerevisiae."
    Loehning C., Rosenbaum C., Ciriacy M.
    Curr. Genet. 24:193-199(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-88; SER-185 AND SER-657, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-235, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Structural analysis of the yeast SWI/SNF chromatin remodeling complex."
    Smith C.L., Horowitz-Scherer R., Flanagan J.F., Woodcock C.L., Peterson C.L.
    Nat. Struct. Biol. 10:141-145(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF THE SWI/SNF COMPLEX, ELECTRON MICROSCOPY OF THE SWI/SNF COMPLEX.
  9. "Proteomic analysis of chromatin-modifying complexes in Saccharomyces cerevisiae identifies novel subunits."
    Lee K.K., Prochasson P., Florens L., Swanson S.K., Washburn M.P., Workman J.L.
    Biochem. Soc. Trans. 32:899-903(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RTT102.
  10. "The RSC chromatin remodeling complex bears an essential fungal-specific protein module with broad functional roles."
    Wilson B., Erdjument-Bromage H., Tempst P., Cairns B.R.
    Genetics 172:795-809(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SWP82.
  11. "Structure and function of the SWIRM domain, a conserved protein module found in chromatin regulatory complexes."
    Da G., Lenkart J., Zhao K., Shiekhattar R., Cairns B.R., Marmorstein R.
    Proc. Natl. Acad. Sci. U.S.A. 103:2057-2062(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF 311-395, MUTAGENESIS OF ASP-374; LYS-383; LYS-387 AND ASN-392.

Entry informationi

Entry nameiSWI3_YEAST
AccessioniPrimary (citable) accession number: P32591
Secondary accession number(s): D6VW13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 8, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3150 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.