SWI/SNF complex subunit SWI3 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P32591 (SWI3_YEAST)

Last modified December 15, 2009. Version 91. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    SWI/SNF complex subunit SWI3
Alternative name(s):
    Transcription regulatory protein SWI3
    Transcription factor TYE2

Gene names

Name:	SWI3
Synonyms:	TYE2
Ordered Locus Names:	YJL176C
ORF Names:	J0495

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	825 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Involved in transcriptional activation. Component of the SWI/SNF complex, an ATP-dependent chromatin-remodeling complex, which is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors.
Subunit structure	Interacts with RTT102, SWP82 and the N-terminus of SNF2. Component of the SWI/SNF global transcription activator complex. The 1.14 MDa SWI/SNF complex is composed of 11 different subunits: one copy each of SWI1, SNF2/SWI2, SNF5, SNF12/SWP73, ARP7/SWP61, ARP9/SWP59; two copies each of SWI3, SNF6, SNF11, SWP82; and three copies of TAF14/SWP29. Ref.8 Ref.9
Subcellular location	Nucleus.
Miscellaneous	Present with 3150 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Contains 1 SANT domain. Contains 1 SWIRM domain.

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Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	ATP-dependent chromatin remodeling Inferred from direct assay. Source: SGD positive regulation of transcription, DNA-dependent Inferred from direct assay. Source: SGD transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	SWI/SNF complex Inferred from direct assay. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW general RNA polymerase II transcription factor activity Traceable author statement. Source: SGD protein binding Ref.9 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 825

825

SWI/SNF complex subunit SWI3

PRO_0000197123

Regions

Domain

305 – 402

SWIRM

Domain

522 – 573

SANT

Domain

694 – 722

Leucine-zipper

Compositional bias

1 – 304

304

Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue

Phosphoserine Ref.5 Ref.6

Modified residue

Phosphothreonine Ref.6

Modified residue

185

Phosphoserine Ref.6

Modified residue

231

Phosphoserine Ref.5 Ref.6

Modified residue

235

Phosphothreonine Ref.5

Modified residue

657

Phosphoserine Ref.6

Experimental info

Mutagenesis

374

D → A: Loss of DNA-binding. Ref.10

Mutagenesis

383

K → D: Loss of DNA-binding; when associated with D-387. Ref.10

Mutagenesis

387

K → D: Loss of DNA-binding; when associated with D-383. Ref.10

Mutagenesis

392

N → A: Loss of DNA-binding. Ref.10

Secondary structure

825

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P32591-1 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: DBA2E2022F15F9A6
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FASTA

825

92,926

        10         20         30         40         50         60 
MENTLGEGST VNASVDVDQH GNDNNSDSNA NAAVAGVANT DTAGEESQQQ DESLKDEATV 

        70         80         90        100        110        120 
PNTRDAESEA ITVTAKQQPT MQANKLDSQE TPSTEESRAQ NVFGQDNEDS DNLFGETESS 

       130        140        150        160        170        180 
VSNNEANTPS IPTNPVDNEN NKPAIKEDST IQDSNGDVKN MEDVKIQKEE EPENNTVIEG 

       190        200        210        220        230        240 
VKEESQPDEN TKEMDEVEED DEDDDQPMIS PDNSIFGDTK SESKQLGNTS SVANTPSEIP 

       250        260        270        280        290        300 
DAHKAEQEDI IEKTESVDKK VDSGEERNEQ EREIMNDHSK SANPKKTTIT RVEPETFEIP 

       310        320        330        340        350        360 
QAHEIVIPSY SKWFNLEKIH SIEVQSLPEF FTNRIPSKTP EVYMRYRNFM VNSYRLNPNE 

       370        380        390        400        410        420 
YFSVTTARRN VSGDAAALFR LHKFLTKWGL INYQVDSKLL PKNIEPPLTS QYSTRHDAPR 

       430        440        450        460        470        480 
GLFPFESYKP SVQLPDMAKL KKMMNTSDSE STLYKYLKES KRKYDEITHP PSTTDDENGD 

       490        500        510        520        530        540 
KNDNGGKMNN EVSTSTSMTG DANLLEEGET SRPLKKVKIL EQIDENWSKE DLQKLLKGIQ 

       550        560        570        580        590        600 
EFGADWYKVA KNVGNKSPEQ CILRFLQLPI EDKFLYGDGN GKGDNDNGLG PLKYAPHLPF 

       610        620        630        640        650        660 
SKSENPVLST IAFLVGLVNP KTVQSMTQRA IQSAESIKSQ KEEISDQKPI EHIKEGSEIA 

       670        680        690        700        710        720 
ISSLGYRSHI FATNEERQMN FLTNELIRLQ MEKLDAKLNH LKKLEKFMEL ERKTLERQQE 

       730        740        750        760        770        780 
NLLIQRLNFN QNSSKIVNVL SKCLNLISDS NINNSSVAEK EEIRSQIDHF KSMLSKPETL 

       790        800        810        820 
SIGKNPFNKP NIETGENHNG QSISNENDVK PISIEAPQFY RYWSA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the yeast SWI1, SWI2, and SWI3 genes, which encode a global activator of transcription." Peterson C.L., Herskowitz I. Cell 68:573-583(1992) [PubMed: 1339306] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Isolation of the TYE2 gene reveals its identity to SWI3 encoding a general transcription factor in Saccharomyces cerevisiae." Loehning C., Rosenbaum C., Ciriacy M. Curr. Genet. 24:193-199(1993) [PubMed: 8221926] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-88; SER-231 AND THR-235, MASS SPECTROMETRY.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-88; THR-91; SER-185; SER-231 AND SER-657, MASS SPECTROMETRY.
[7]	"Structural analysis of the yeast SWI/SNF chromatin remodeling complex." Smith C.L., Horowitz-Scherer R., Flanagan J.F., Woodcock C.L., Peterson C.L. Nat. Struct. Biol. 10:141-145(2003) [PubMed: 12524530] [Abstract] Cited for: 3D-STRUCTURE MODELING OF THE SWI/SNF COMPLEX, ELECTRON MICROSCOPY OF THE SWI/SNF COMPLEX.
[8]	"Proteomic analysis of chromatin-modifying complexes in Saccharomyces cerevisiae identifies novel subunits." Lee K.K., Prochasson P., Florens L., Swanson S.K., Washburn M.P., Workman J.L. Biochem. Soc. Trans. 32:899-903(2004) [PubMed: 15506919] [Abstract] Cited for: INTERACTION WITH RTT102.
[9]	"The RSC chromatin remodeling complex bears an essential fungal-specific protein module with broad functional roles." Wilson B., Erdjument-Bromage H., Tempst P., Cairns B.R. Genetics 172:795-809(2006) [PubMed: 16204215] [Abstract] Cited for: INTERACTION WITH SWP82.
[10]	"Structure and function of the SWIRM domain, a conserved protein module found in chromatin regulatory complexes." Da G., Lenkart J., Zhao K., Shiekhattar R., Cairns B.R., Marmorstein R. Proc. Natl. Acad. Sci. U.S.A. 103:2057-2062(2006) [PubMed: 16461455] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF 311-395, MUTAGENESIS OF ASP-374; LYS-383; LYS-387 AND ASN-392.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

M84390 Genomic DNA. Translation: AAA35136.1.
X56792 Genomic DNA. Translation: CAA40112.1.
Z49451 Genomic DNA. Translation: CAA89470.1.

PIR

S26706.

RefSeq

NP_012359.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FQ3	X-ray	1.40	A	309-398	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2471N.

IntAct

P32591. 28 interactions.

STRING

P32591.

Proteomic databases

PeptideAtlas

P32591.

Genome annotation databases

Ensembl

YJL176C; YJL176C; YJL176C; Saccharomyces cerevisiae. [Genome view]

GeneID

853264.

KEGG

sce:YJL176C.

NMPDR

fig|4932.3.peg.3323.

Organism-specific databases

CYGD

YJL176c.

SGD

S000003712. SWI3.

Phylogenomic databases

HOGENOM

HBG202280.

OrthoDB

EOG9KSR2K.

Gene expression databases

ArrayExpress

P32591.

Genevestigator

P32591.

GermOnline

YJL176C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR009057. Homeodomain-like.
IPR014778. Myb_DNA-bd.
IPR001005. SANT_DNA-bd.
IPR017884. SANT_eukarya.
IPR007526. SWIRM.
[Graphical view]

Pfam

PF00249. Myb_DNA-binding. 1 hit.
PF04433. SWIRM. 1 hit.
[Graphical view]

SMART

SM00717. SANT. 1 hit.
[Graphical view]

PROSITE

PS51293. SANT. 1 hit.
PS50934. SWIRM. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

973526.

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Entry information

Entry name

SWI3_YEAST

Accession

Primary (citable) accession number: P32591

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	October 1, 1993
Last modified:	December 15, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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