P32590 (HSP79_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Heat shock protein homolog SSE2

Gene names

Name:	SSE2
Synonyms:	HSP
Ordered Locus Names:	YBR169C
ORF Names:	YBR1221

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	693 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a calcium-dependent calmodulin-binding activity.
Induction	By upshift to 37 degrees Celsius.
Miscellaneous	Present with 6300 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Biological process	Stress response
Ligand	ATP-binding Calmodulin-binding Nucleotide-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein refolding Inferred from genetic interaction PubMed 16688211. Source: SGD response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenyl-nucleotide exchange factor activity Inferred from direct assay PubMed 16688212. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 693	692	Heat shock protein homolog SSE2		PRO_0000078397
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	384	1	Phosphoserine Ref.7 Ref.8 Ref.9
	Modified residue	386	1	Phosphothreonine Ref.9
Experimental info
	Sequence conflict	160 – 167	8	Missing in CAA51027. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P32590 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: D6BC9656D93895C2	FASTA	693	77,621
	10 20 30 40 50 60 MSTPFGLDLG NNNSVLAVAR NRGIDVVVNE VSNRSTPSLV GFGPRNRYLG ESGKTKQTSN 70 80 90 100 110 120 VKNTVENLKR IIGLKFKDPE FDIENKFFTS KLVQLKNGKV GVEVEFGGKT HVFSATQLTA 130 140 150 160 170 180 MFIDKVKHTV QEETKSSITD VCLAVPVWYS EEQRYNIADA ARIAGLNPVR IVNDVTAAAV 190 200 210 220 230 240 SYGVFKNDLP GPEEKPRIIG LVDIGHSTYT CSIMAFRKGE MKVLGTAYDK HFGGRDFDRA 250 260 270 280 290 300 ITEHFADQFK DKYKIDIRKN PKAYNRILIA AEKLKKVLSA NTTAPFSVES VMDDIDVSSQ 310 320 330 340 350 360 LSREELEELV EPLLKRVTYP ITNALAQAKL TVNDIDFVEI IGGTTRIPVL KKSISDVFGK 370 380 390 400 410 420 PLSSTLNQDE AVAKGAAFIC AIHSPTLRVR PFKFEDIDPY SVSYTWDKQV DDEDRLEVFP 430 440 450 460 470 480 ANSSYPSTKL ITLHRTGDFS MKAVYTHPSK LPKGTSTTIA KWSFTGVKVP KDQDFIPVKV 490 500 510 520 530 540 KLRCDPSGLH IIENAYTTED ITVQEPVPLP EDAPEDAEPQ FKEVTKTIKK DVLGMTAKTF 550 560 570 580 590 600 ALNPVELNDL IEKENELRNQ DKLVAETEDR KNALEEYIYT LRAKLDDEYS DFASDAEKEK 610 620 630 640 650 660 LKNMLATTEN WLYGDGDDST KAKYIAKYEE LASLGNIIRG RYLAKEEEKR QALRANQETS 670 680 690 KMNDIAEKLA EQRRARAASD DSDDNNDENM DLD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of SSE1 and SSE2, new members of the yeast HSP70 multigene family." Mukai H., Kuno T., Tanaka H., Hirata D., Miyakawa T., Tanaka C. Gene 132:57-66(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 204626 / S288c / A364A.
[2]	"Suppression of a sec63 mutation identifies a novel component of the yeast endoplasmic reticulum translocation apparatus." Kurihara T., Silver P.A. Mol. Biol. Cell 4:919-930(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-384, MASS SPECTROMETRY. Strain: YAL6B.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-384, MASS SPECTROMETRY. Strain: ADR376.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-384 AND THR-386, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D38369 mRNA. Translation: BAA07450.1. D38371 Genomic DNA. Translation: BAA07452.1. X72224 Genomic DNA. Translation: CAA51027.1. Z36038 Genomic DNA. Translation: CAA85130.1. AY692880 Genomic DNA. Translation: AAT92899.1. BK006936 Genomic DNA. Translation: DAA07285.1.
PIR	JN0843.
RefSeq	NP_009728.1. NM_001178517.1.
3D structure databases
ProteinModelPortal	P32590.
SMR	P32590. Positions 1-649.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4862N.
IntAct	P32590. 8 interactions.
MINT	MINT-500770.
STRING	4932.YBR169C.
2D gel databases
UCD-2DPAGE	P32590.
Proteomic databases
PaxDb	P32590.
PeptideAtlas	P32590.
PRIDE	P32590.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR169C; YBR169C; YBR169C.
GeneID	852467.
KEGG	sce:YBR169C.
Organism-specific databases
SGD	S000000373. SSE2.
Phylogenomic databases
eggNOG	COG0443.
GeneTree	ENSGT00390000016919.
HOGENOM	HOG000228138.
KO	K09485.
OMA	WEIRSSA.
OrthoDB	EOG490BJ6.
Enzyme and pathway databases
BioCyc	YEAST:G3O-29117-MONOMER.
Gene expression databases
Genevestigator	P32590.
GermOnline	YBR169C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018181. Heat_shock_70_CS. IPR013126. Hsp_70_fam. [Graphical view]
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
PROSITE	PS00297. HSP70_1. False negative. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971417.

Entry information

Entry name

HSP79_YEAST

Accession

Primary (citable) accession number: P32590
Secondary accession number(s): D6VQG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 115 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program