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P32586

- PYP2_SCHPO

UniProt

P32586 - PYP2_SCHPO

Protein

Tyrosine-protein phosphatase 2

Gene

pyp2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Plays a role in inhibiting the onset of mitosis. Dephosphorylates sty1/spc1 and wis1/spc2/sty2.

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei630 – 6301Phosphocysteine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. MAP kinase tyrosine phosphatase activity Source: PomBase
    2. protein binding Source: PomBase
    3. protein tyrosine phosphatase activity Source: PomBase

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. negative regulation of G2/M transition of mitotic cell cycle Source: PomBase
    3. negative regulation of stress-activated MAPK cascade Source: PomBase
    4. peptidyl-tyrosine dephosphorylation Source: PomBase
    5. peptidyl-tyrosine dephosphorylation involved in inactivation of protein kinase activity Source: GOC
    6. protein dephosphorylation Source: PomBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein phosphatase 2 (EC:3.1.3.48)
    Alternative name(s):
    Protein-tyrosine phosphatase 2
    Short name:
    PTPase 2
    Gene namesi
    Name:pyp2
    ORF Names:SPAC19D5.01
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC19D5.01.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: PomBase
    2. cytosol Source: PomBase
    3. mitochondrion Source: PomBase
    4. nucleus Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 711711Tyrosine-protein phosphatase 2PRO_0000094859Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    BioGridi278675. 58 interactions.
    MINTiMINT-4688417.
    STRINGi4896.SPAC19D5.01-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP32586.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 130110RhodanesePROSITE-ProRule annotationAdd
    BLAST
    Domaini433 – 698266Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 rhodanese domain.PROSITE-ProRule annotation
    Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5599.
    KOiK01104.
    OrthoDBiEOG7R573B.
    PhylomeDBiP32586.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    3.90.190.10. 1 hit.
    InterProiIPR029021. Prot-tyrosine_phosphatase-like.
    IPR001763. Rhodanese-like_dom.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view]
    PfamiPF00581. Rhodanese. 1 hit.
    PF00102. Y_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00700. PRTYPHPHTASE.
    SMARTiSM00194. PTPc. 1 hit.
    SM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    SSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32586-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLHLLSKDEF NSTLKSFEEQ TESVSWIIDL RLHSKYAVSH IKNAINVSLP    50
    TALLRRPSFD IGKVFACIKC NVKVSLDEIN AIFLYDSSMA GMNRIYDLVQ 100
    KFRRGGYSKK IYLLSNGFEA FASSHPDAIV STEMVKESVP YKIDINENCK 150
    LDILHLSDPS AVSTPISPDY SFPLRVPINI PPPLCTPSVV SDTFSEFASH 200
    AEYPGFSGLT PFSIHSPTAS SVRSCQSIYG SPLSPPNSAF QAEMPYFPIS 250
    PAISCASSCP STPDEQKNFF IVGNAPQQTP ARPSLRSVPS YPSSNNQRRP 300
    SASRVRSFSN YVKSSNVVNP SLSQASLEII PRKSMKRDSN AQNDGTSTMT 350
    SKLKPSVGLS NTRDAPKPGG LRRANKPCFN KETKGSIFSK ENKGPFTCNP 400
    WGAKKVSPPP CEVLADLNTA SIFYKFKRLE EMEMTRSLAF NDSKSDWCCL 450
    ASSRSTSISR KNRYTDIVPY DKTRVRLAVP KGCSDYINAS HIDVGNKKYI 500
    ACQAPKPGTL LDFWEMVWHN SGTNGVIVML TNLYEAGSEK CSQYWPDNKD 550
    HALCLEGGLR ISVQKYETFE DLKVNTHLFR LDKPNGPPKY IHHFWVHTWF 600
    DKTHPDIESI TGIIRCIDKV PNDGPMFVHC SAGVGRTGTF IAVDQILQVP 650
    KNILPKTTNL EDSKDFIFNC VNSLRSQRMK MVQNFEQFKF LYDVVDYLNS 700
    GVNQASKPLM T 711
    Length:711
    Mass (Da):79,356
    Last modified:October 1, 1993 - v1
    Checksum:i15A12CDAE13D341E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti271 – 2755IVGNA → NRRQC in CAA42167. (PubMed:1448087)Curated
    Sequence conflicti487 – 4871I → M(PubMed:1668885)Curated
    Sequence conflicti515 – 5151E → N(PubMed:1668885)Curated
    Sequence conflicti519 – 5191H → D(PubMed:1668885)Curated
    Sequence conflicti636 – 6361R → P in CAA42167. (PubMed:1448087)Curated
    Sequence conflicti668 – 6703FNC → IQL in CAA42167. (PubMed:1448087)Curated
    Sequence conflicti675 – 6762RS → DT in CAA42167. (PubMed:1448087)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S51320 Genomic DNA. Translation: AAB24544.1.
    X59599 mRNA. Translation: CAA42167.1.
    AB027789 Genomic DNA. Translation: BAA87093.1.
    CU329670 Genomic DNA. Translation: CAB16711.1.
    PIRiS28391.
    T45160.
    RefSeqiNP_594899.1. NM_001020328.2.

    Genome annotation databases

    EnsemblFungiiSPAC19D5.01.1; SPAC19D5.01.1:pep; SPAC19D5.01.
    GeneIDi2542200.
    KEGGispo:SPAC19D5.01.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S51320 Genomic DNA. Translation: AAB24544.1 .
    X59599 mRNA. Translation: CAA42167.1 .
    AB027789 Genomic DNA. Translation: BAA87093.1 .
    CU329670 Genomic DNA. Translation: CAB16711.1 .
    PIRi S28391.
    T45160.
    RefSeqi NP_594899.1. NM_001020328.2.

    3D structure databases

    ProteinModelPortali P32586.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 278675. 58 interactions.
    MINTi MINT-4688417.
    STRINGi 4896.SPAC19D5.01-1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC19D5.01.1 ; SPAC19D5.01.1:pep ; SPAC19D5.01 .
    GeneIDi 2542200.
    KEGGi spo:SPAC19D5.01.

    Organism-specific databases

    PomBasei SPAC19D5.01.

    Phylogenomic databases

    eggNOGi COG5599.
    KOi K01104.
    OrthoDBi EOG7R573B.
    PhylomeDBi P32586.

    Miscellaneous databases

    NextBioi 20803270.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    3.90.190.10. 1 hit.
    InterProi IPR029021. Prot-tyrosine_phosphatase-like.
    IPR001763. Rhodanese-like_dom.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view ]
    Pfami PF00581. Rhodanese. 1 hit.
    PF00102. Y_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00700. PRTYPHPHTASE.
    SMARTi SM00194. PTPc. 1 hit.
    SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Negative regulation of mitosis by two functionally overlapping PTPases in fission yeast."
      Millar J.B.A., Russell P., Dixon J.E., Guan K.L.
      EMBO J. 11:4943-4952(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The fission yeast genes pyp1+ and pyp2+ encode protein tyrosine phosphatases that negatively regulate mitosis."
      Ottilie S., Chernoff J., Hannig G., Hoffman C.S., Erikson R.L.
      Mol. Cell. Biol. 12:5571-5580(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    4. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
      Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
      Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 285-437.
      Strain: ATCC 38364 / 968.
    5. "Protein phosphatases in cell division: how vital are they?"
      Yanagida M., Yamano H., Stone E.M., Kinoshita N., Yoshida T., Shiozaki K.
      Princess Takamatsu Symp. 22:137-144(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE OF 485-519.
    6. "Pyp1 and Pyp2 PTPases dephosphorylate an osmosensing MAP kinase controlling cell size at division in fission yeast."
      Millar J.B.A., Buck V., Wilkinson M.G.
      Genes Dev. 9:2117-2130(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiPYP2_SCHPO
    AccessioniPrimary (citable) accession number: P32586
    Secondary accession number(s): Q9UR59, Q9UU64
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3