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Protein

Protein-S-isoprenylcysteine O-methyltransferase

Gene

STE14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates C-terminal methylation of the isoprenylated C-terminal cysteine in A-factor mating pheromone and Ras proteins.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.

GO - Molecular functioni

GO - Biological processi

  • C-terminal protein methylation Source: SGD
  • peptide pheromone maturation Source: SGD
  • response to pheromone Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Pheromone response

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-29953-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-S-isoprenylcysteine O-methyltransferase (EC:2.1.1.100)
Alternative name(s):
Isoprenylcysteine carboxylmethyltransferase
Prenylated protein carboxyl methyltransferase
Short name:
PPMT
Prenylcysteine carboxyl methyltransferase
Short name:
pcCMT
Gene namesi
Name:STE14
Ordered Locus Names:YDR410C
ORF Names:D9461.1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IV

Organism-specific databases

CYGDiYDR410c.
EuPathDBiFungiDB:YDR410C.
SGDiS000002818. STE14.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2323ExtracellularSequence AnalysisAdd
BLAST
Transmembranei24 – 4421HelicalSequence AnalysisAdd
BLAST
Topological domaini45 – 473CytoplasmicSequence Analysis
Transmembranei48 – 6821HelicalSequence AnalysisAdd
BLAST
Topological domaini69 – 8820ExtracellularSequence AnalysisAdd
BLAST
Transmembranei89 – 10921HelicalSequence AnalysisAdd
BLAST
Topological domaini110 – 1101CytoplasmicSequence Analysis
Transmembranei111 – 13121HelicalSequence AnalysisAdd
BLAST
Topological domaini132 – 18251ExtracellularSequence AnalysisAdd
BLAST
Transmembranei183 – 20321HelicalSequence AnalysisAdd
BLAST
Topological domaini204 – 23936CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • nuclear inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 239239Protein-S-isoprenylcysteine O-methyltransferasePRO_0000209899Add
BLAST

Proteomic databases

MaxQBiP32584.
PaxDbiP32584.

Interactioni

Protein-protein interaction databases

BioGridi32470. 15 interactions.
DIPiDIP-2678N.
IntActiP32584. 3 interactions.
MINTiMINT-1651790.

Structurei

3D structure databases

ProteinModelPortaliP32584.
SMRiP32584. Positions 144-237.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2020.
GeneTreeiENSGT00390000017394.
HOGENOMiHOG000213961.
InParanoidiP32584.
KOiK00587.
OMAiNFNHHVQ.
OrthoDBiEOG7WHHPD.

Family and domain databases

InterProiIPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view]
PfamiPF04140. ICMT. 1 hit.
[Graphical view]
PROSITEiPS51564. SAM_ICMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32584-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHQDFQEDEH EYPDIRRNPL HEVTMTSYIL GILLGIFVGL FPQIRFKNFN
60 70 80 90 100
LFIIALSLFH FLEYYITAKY NPLKVHSESF LLNNGKSYMA AHSFAILECL
110 120 130 140 150
VESFLFPDLK IFSYSLATKL CTVLGCLLVI LGQYTRTIAM HTAGHSFSHI
160 170 180 190 200
VKTKKESDHV LVKTGVYSWS RHPSYLGFFW WAIGTQLLLL NPLSLVIFIF
210 220 230
VLWKFFSDRI RVEEKYLIEF FSAEYIEYKN KVGVGIPFI
Length:239
Mass (Da):27,888
Last modified:October 1, 1993 - v1
Checksum:i189829AFD0BD6BD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07952 Unassigned DNA. Translation: AAA16520.1.
L15442 Unassigned DNA. Translation: AAA16840.1.
U33007 Genomic DNA. Translation: AAB64880.1.
BK006938 Genomic DNA. Translation: DAA12252.1.
PIRiS37604.
RefSeqiNP_010698.1. NM_001180718.1.

Genome annotation databases

EnsemblFungiiYDR410C; YDR410C; YDR410C.
GeneIDi852019.
KEGGisce:YDR410C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07952 Unassigned DNA. Translation: AAA16520.1.
L15442 Unassigned DNA. Translation: AAA16840.1.
U33007 Genomic DNA. Translation: AAB64880.1.
BK006938 Genomic DNA. Translation: DAA12252.1.
PIRiS37604.
RefSeqiNP_010698.1. NM_001180718.1.

3D structure databases

ProteinModelPortaliP32584.
SMRiP32584. Positions 144-237.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32470. 15 interactions.
DIPiDIP-2678N.
IntActiP32584. 3 interactions.
MINTiMINT-1651790.

Chemistry

BindingDBiP32584.
ChEMBLiCHEMBL4385.

Proteomic databases

MaxQBiP32584.
PaxDbiP32584.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR410C; YDR410C; YDR410C.
GeneIDi852019.
KEGGisce:YDR410C.

Organism-specific databases

CYGDiYDR410c.
EuPathDBiFungiDB:YDR410C.
SGDiS000002818. STE14.

Phylogenomic databases

eggNOGiCOG2020.
GeneTreeiENSGT00390000017394.
HOGENOMiHOG000213961.
InParanoidiP32584.
KOiK00587.
OMAiNFNHHVQ.
OrthoDBiEOG7WHHPD.

Enzyme and pathway databases

BioCyciYEAST:G3O-29953-MONOMER.

Miscellaneous databases

NextBioi970234.
PROiP32584.

Family and domain databases

InterProiIPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view]
PfamiPF04140. ICMT. 1 hit.
[Graphical view]
PROSITEiPS51564. SAM_ICMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Saccharomyces cerevisiae STE14 gene encodes a methyltransferase that mediates C-terminal methylation of a-factor and RAS proteins."
    Hrycyna C.A., Sapperstein S.K., Clarke S., Michaelis S.
    EMBO J. 10:1699-1709(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Isolation and DNA sequence of the STE14 gene encoding farnesyl cysteine: carboxyl methyltransferase."
    Ashby M.N., Errada P.R., Boyartchuk V.L., Rine J.
    Yeast 9:907-913(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  3. "Nucleotide sequence of the yeast STE14 gene, which encodes farnesylcysteine carboxyl methyltransferase, and demonstration of its essential role in a-factor export."
    Sapperstein S., Berkower C., Michaelis S.
    Mol. Cell. Biol. 14:1438-1449(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, FUNCTION.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiSTE14_YEAST
AccessioniPrimary (citable) accession number: P32584
Secondary accession number(s): D6VT42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: July 22, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2690 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.