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P32584

- STE14_YEAST

UniProt

P32584 - STE14_YEAST

Protein

Protein-S-isoprenylcysteine O-methyltransferase

Gene

STE14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Oct 1993)
      Previous versions | rss
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    Functioni

    Mediates C-terminal methylation of the isoprenylated C-terminal cysteine in A-factor mating pheromone and Ras proteins.1 Publication

    Catalytic activityi

    S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.

    GO - Molecular functioni

    1. protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Source: SGD

    GO - Biological processi

    1. C-terminal protein methylation Source: SGD
    2. peptide pheromone maturation Source: SGD
    3. response to pheromone Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Pheromone response

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29953-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein-S-isoprenylcysteine O-methyltransferase (EC:2.1.1.100)
    Alternative name(s):
    Isoprenylcysteine carboxylmethyltransferase
    Prenylated protein carboxyl methyltransferase
    Short name:
    PPMT
    Prenylcysteine carboxyl methyltransferase
    Short name:
    pcCMT
    Gene namesi
    Name:STE14
    Ordered Locus Names:YDR410C
    ORF Names:D9461.1
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR410c.
    SGDiS000002818. STE14.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: SGD
    2. integral component of membrane Source: UniProtKB-KW
    3. nuclear inner membrane Source: SGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 239239Protein-S-isoprenylcysteine O-methyltransferasePRO_0000209899Add
    BLAST

    Proteomic databases

    MaxQBiP32584.
    PaxDbiP32584.

    Expressioni

    Gene expression databases

    GenevestigatoriP32584.

    Interactioni

    Protein-protein interaction databases

    BioGridi32470. 15 interactions.
    DIPiDIP-2678N.
    IntActiP32584. 3 interactions.
    MINTiMINT-1651790.
    STRINGi4932.YDR410C.

    Structurei

    3D structure databases

    ProteinModelPortaliP32584.
    SMRiP32584. Positions 144-237.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2323ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini45 – 473CytoplasmicSequence Analysis
    Topological domaini69 – 8820ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini110 – 1101CytoplasmicSequence Analysis
    Topological domaini132 – 18251ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini204 – 23936CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei24 – 4421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei48 – 6821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei89 – 10921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei111 – 13121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei183 – 20321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2020.
    GeneTreeiENSGT00390000017394.
    HOGENOMiHOG000213961.
    KOiK00587.
    OMAiGSAIVEC.
    OrthoDBiEOG7WHHPD.

    Family and domain databases

    InterProiIPR007269. ICMT_MeTrfase.
    IPR025770. PPMT_MeTrfase.
    [Graphical view]
    PfamiPF04140. ICMT. 1 hit.
    [Graphical view]
    PROSITEiPS51564. SAM_ICMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P32584-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHQDFQEDEH EYPDIRRNPL HEVTMTSYIL GILLGIFVGL FPQIRFKNFN    50
    LFIIALSLFH FLEYYITAKY NPLKVHSESF LLNNGKSYMA AHSFAILECL 100
    VESFLFPDLK IFSYSLATKL CTVLGCLLVI LGQYTRTIAM HTAGHSFSHI 150
    VKTKKESDHV LVKTGVYSWS RHPSYLGFFW WAIGTQLLLL NPLSLVIFIF 200
    VLWKFFSDRI RVEEKYLIEF FSAEYIEYKN KVGVGIPFI 239
    Length:239
    Mass (Da):27,888
    Last modified:October 1, 1993 - v1
    Checksum:i189829AFD0BD6BD5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07952 Unassigned DNA. Translation: AAA16520.1.
    L15442 Unassigned DNA. Translation: AAA16840.1.
    U33007 Genomic DNA. Translation: AAB64880.1.
    BK006938 Genomic DNA. Translation: DAA12252.1.
    PIRiS37604.
    RefSeqiNP_010698.1. NM_001180718.1.

    Genome annotation databases

    EnsemblFungiiYDR410C; YDR410C; YDR410C.
    GeneIDi852019.
    KEGGisce:YDR410C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L07952 Unassigned DNA. Translation: AAA16520.1 .
    L15442 Unassigned DNA. Translation: AAA16840.1 .
    U33007 Genomic DNA. Translation: AAB64880.1 .
    BK006938 Genomic DNA. Translation: DAA12252.1 .
    PIRi S37604.
    RefSeqi NP_010698.1. NM_001180718.1.

    3D structure databases

    ProteinModelPortali P32584.
    SMRi P32584. Positions 144-237.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32470. 15 interactions.
    DIPi DIP-2678N.
    IntActi P32584. 3 interactions.
    MINTi MINT-1651790.
    STRINGi 4932.YDR410C.

    Chemistry

    BindingDBi P32584.
    ChEMBLi CHEMBL4385.

    Proteomic databases

    MaxQBi P32584.
    PaxDbi P32584.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR410C ; YDR410C ; YDR410C .
    GeneIDi 852019.
    KEGGi sce:YDR410C.

    Organism-specific databases

    CYGDi YDR410c.
    SGDi S000002818. STE14.

    Phylogenomic databases

    eggNOGi COG2020.
    GeneTreei ENSGT00390000017394.
    HOGENOMi HOG000213961.
    KOi K00587.
    OMAi GSAIVEC.
    OrthoDBi EOG7WHHPD.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29953-MONOMER.

    Miscellaneous databases

    NextBioi 970234.
    PROi P32584.

    Gene expression databases

    Genevestigatori P32584.

    Family and domain databases

    InterProi IPR007269. ICMT_MeTrfase.
    IPR025770. PPMT_MeTrfase.
    [Graphical view ]
    Pfami PF04140. ICMT. 1 hit.
    [Graphical view ]
    PROSITEi PS51564. SAM_ICMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Saccharomyces cerevisiae STE14 gene encodes a methyltransferase that mediates C-terminal methylation of a-factor and RAS proteins."
      Hrycyna C.A., Sapperstein S.K., Clarke S., Michaelis S.
      EMBO J. 10:1699-1709(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "Isolation and DNA sequence of the STE14 gene encoding farnesyl cysteine: carboxyl methyltransferase."
      Ashby M.N., Errada P.R., Boyartchuk V.L., Rine J.
      Yeast 9:907-913(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    3. "Nucleotide sequence of the yeast STE14 gene, which encodes farnesylcysteine carboxyl methyltransferase, and demonstration of its essential role in a-factor export."
      Sapperstein S., Berkower C., Michaelis S.
      Mol. Cell. Biol. 14:1438-1449(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE, FUNCTION.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.

    Entry informationi

    Entry nameiSTE14_YEAST
    AccessioniPrimary (citable) accession number: P32584
    Secondary accession number(s): D6VT42
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: October 1, 1993
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2690 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3