Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P32584

- STE14_YEAST

UniProt

P32584 - STE14_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Protein-S-isoprenylcysteine O-methyltransferase

Gene

STE14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Mediates C-terminal methylation of the isoprenylated C-terminal cysteine in A-factor mating pheromone and Ras proteins.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.

GO - Molecular functioni

  1. protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Source: SGD

GO - Biological processi

  1. C-terminal protein methylation Source: SGD
  2. peptide pheromone maturation Source: SGD
  3. response to pheromone Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Pheromone response

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-29953-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-S-isoprenylcysteine O-methyltransferase (EC:2.1.1.100)
Alternative name(s):
Isoprenylcysteine carboxylmethyltransferase
Prenylated protein carboxyl methyltransferase
Short name:
PPMT
Prenylcysteine carboxyl methyltransferase
Short name:
pcCMT
Gene namesi
Name:STE14
Ordered Locus Names:YDR410C
ORF Names:D9461.1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDR410c.
SGDiS000002818. STE14.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: SGD
  2. integral component of membrane Source: UniProtKB-KW
  3. nuclear inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 239239Protein-S-isoprenylcysteine O-methyltransferasePRO_0000209899Add
BLAST

Proteomic databases

MaxQBiP32584.
PaxDbiP32584.

Expressioni

Gene expression databases

GenevestigatoriP32584.

Interactioni

Protein-protein interaction databases

BioGridi32470. 15 interactions.
DIPiDIP-2678N.
IntActiP32584. 3 interactions.
MINTiMINT-1651790.
STRINGi4932.YDR410C.

Structurei

3D structure databases

ProteinModelPortaliP32584.
SMRiP32584. Positions 144-237.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2323ExtracellularSequence AnalysisAdd
BLAST
Topological domaini45 – 473CytoplasmicSequence Analysis
Topological domaini69 – 8820ExtracellularSequence AnalysisAdd
BLAST
Topological domaini110 – 1101CytoplasmicSequence Analysis
Topological domaini132 – 18251ExtracellularSequence AnalysisAdd
BLAST
Topological domaini204 – 23936CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei24 – 4421HelicalSequence AnalysisAdd
BLAST
Transmembranei48 – 6821HelicalSequence AnalysisAdd
BLAST
Transmembranei89 – 10921HelicalSequence AnalysisAdd
BLAST
Transmembranei111 – 13121HelicalSequence AnalysisAdd
BLAST
Transmembranei183 – 20321HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2020.
GeneTreeiENSGT00390000017394.
HOGENOMiHOG000213961.
InParanoidiP32584.
KOiK00587.
OMAiGSAIVEC.
OrthoDBiEOG7WHHPD.

Family and domain databases

InterProiIPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view]
PfamiPF04140. ICMT. 1 hit.
[Graphical view]
PROSITEiPS51564. SAM_ICMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32584 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHQDFQEDEH EYPDIRRNPL HEVTMTSYIL GILLGIFVGL FPQIRFKNFN
60 70 80 90 100
LFIIALSLFH FLEYYITAKY NPLKVHSESF LLNNGKSYMA AHSFAILECL
110 120 130 140 150
VESFLFPDLK IFSYSLATKL CTVLGCLLVI LGQYTRTIAM HTAGHSFSHI
160 170 180 190 200
VKTKKESDHV LVKTGVYSWS RHPSYLGFFW WAIGTQLLLL NPLSLVIFIF
210 220 230
VLWKFFSDRI RVEEKYLIEF FSAEYIEYKN KVGVGIPFI
Length:239
Mass (Da):27,888
Last modified:October 1, 1993 - v1
Checksum:i189829AFD0BD6BD5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07952 Unassigned DNA. Translation: AAA16520.1.
L15442 Unassigned DNA. Translation: AAA16840.1.
U33007 Genomic DNA. Translation: AAB64880.1.
BK006938 Genomic DNA. Translation: DAA12252.1.
PIRiS37604.
RefSeqiNP_010698.1. NM_001180718.1.

Genome annotation databases

EnsemblFungiiYDR410C; YDR410C; YDR410C.
GeneIDi852019.
KEGGisce:YDR410C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L07952 Unassigned DNA. Translation: AAA16520.1 .
L15442 Unassigned DNA. Translation: AAA16840.1 .
U33007 Genomic DNA. Translation: AAB64880.1 .
BK006938 Genomic DNA. Translation: DAA12252.1 .
PIRi S37604.
RefSeqi NP_010698.1. NM_001180718.1.

3D structure databases

ProteinModelPortali P32584.
SMRi P32584. Positions 144-237.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32470. 15 interactions.
DIPi DIP-2678N.
IntActi P32584. 3 interactions.
MINTi MINT-1651790.
STRINGi 4932.YDR410C.

Chemistry

BindingDBi P32584.
ChEMBLi CHEMBL4385.

Proteomic databases

MaxQBi P32584.
PaxDbi P32584.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDR410C ; YDR410C ; YDR410C .
GeneIDi 852019.
KEGGi sce:YDR410C.

Organism-specific databases

CYGDi YDR410c.
SGDi S000002818. STE14.

Phylogenomic databases

eggNOGi COG2020.
GeneTreei ENSGT00390000017394.
HOGENOMi HOG000213961.
InParanoidi P32584.
KOi K00587.
OMAi GSAIVEC.
OrthoDBi EOG7WHHPD.

Enzyme and pathway databases

BioCyci YEAST:G3O-29953-MONOMER.

Miscellaneous databases

NextBioi 970234.
PROi P32584.

Gene expression databases

Genevestigatori P32584.

Family and domain databases

InterProi IPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view ]
Pfami PF04140. ICMT. 1 hit.
[Graphical view ]
PROSITEi PS51564. SAM_ICMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Saccharomyces cerevisiae STE14 gene encodes a methyltransferase that mediates C-terminal methylation of a-factor and RAS proteins."
    Hrycyna C.A., Sapperstein S.K., Clarke S., Michaelis S.
    EMBO J. 10:1699-1709(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Isolation and DNA sequence of the STE14 gene encoding farnesyl cysteine: carboxyl methyltransferase."
    Ashby M.N., Errada P.R., Boyartchuk V.L., Rine J.
    Yeast 9:907-913(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  3. "Nucleotide sequence of the yeast STE14 gene, which encodes farnesylcysteine carboxyl methyltransferase, and demonstration of its essential role in a-factor export."
    Sapperstein S., Berkower C., Michaelis S.
    Mol. Cell. Biol. 14:1438-1449(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, FUNCTION.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiSTE14_YEAST
AccessioniPrimary (citable) accession number: P32584
Secondary accession number(s): D6VT42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: October 29, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2690 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3