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Protein

Protein-S-isoprenylcysteine O-methyltransferase

Gene

STE14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates C-terminal methylation of the isoprenylated C-terminal cysteine in A-factor mating pheromone and Ras proteins.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.

GO - Molecular functioni

GO - Biological processi

  • C-terminal protein methylation Source: SGD
  • peptide pheromone maturation Source: SGD
  • response to pheromone Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Pheromone response

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-29953-MONOMER.
ReactomeiR-SCE-163841. Gamma carboxylation, hypusine formation and arylsulfatase activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-S-isoprenylcysteine O-methyltransferase (EC:2.1.1.100)
Alternative name(s):
Isoprenylcysteine carboxylmethyltransferase
Prenylated protein carboxyl methyltransferase
Short name:
PPMT
Prenylcysteine carboxyl methyltransferase
Short name:
pcCMT
Gene namesi
Name:STE14
Ordered Locus Names:YDR410C
ORF Names:D9461.1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR410C.
SGDiS000002818. STE14.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 23ExtracellularSequence analysisAdd BLAST23
Transmembranei24 – 44HelicalSequence analysisAdd BLAST21
Topological domaini45 – 47CytoplasmicSequence analysis3
Transmembranei48 – 68HelicalSequence analysisAdd BLAST21
Topological domaini69 – 88ExtracellularSequence analysisAdd BLAST20
Transmembranei89 – 109HelicalSequence analysisAdd BLAST21
Topological domaini110CytoplasmicSequence analysis1
Transmembranei111 – 131HelicalSequence analysisAdd BLAST21
Topological domaini132 – 182ExtracellularSequence analysisAdd BLAST51
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21
Topological domaini204 – 239CytoplasmicSequence analysisAdd BLAST36

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • nuclear inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4385.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002098991 – 239Protein-S-isoprenylcysteine O-methyltransferaseAdd BLAST239

Proteomic databases

MaxQBiP32584.
PRIDEiP32584.

Interactioni

Protein-protein interaction databases

BioGridi32470. 15 interactors.
DIPiDIP-2678N.
IntActiP32584. 3 interactors.
MINTiMINT-1651790.

Chemistry databases

BindingDBiP32584.

Structurei

3D structure databases

ProteinModelPortaliP32584.
SMRiP32584.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000017394.
HOGENOMiHOG000213961.
InParanoidiP32584.
KOiK00587.
OMAiLPFIRGY.
OrthoDBiEOG092C5DVA.

Family and domain databases

InterProiIPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view]
PfamiPF04140. ICMT. 1 hit.
[Graphical view]
PROSITEiPS51564. SAM_ICMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32584-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHQDFQEDEH EYPDIRRNPL HEVTMTSYIL GILLGIFVGL FPQIRFKNFN
60 70 80 90 100
LFIIALSLFH FLEYYITAKY NPLKVHSESF LLNNGKSYMA AHSFAILECL
110 120 130 140 150
VESFLFPDLK IFSYSLATKL CTVLGCLLVI LGQYTRTIAM HTAGHSFSHI
160 170 180 190 200
VKTKKESDHV LVKTGVYSWS RHPSYLGFFW WAIGTQLLLL NPLSLVIFIF
210 220 230
VLWKFFSDRI RVEEKYLIEF FSAEYIEYKN KVGVGIPFI
Length:239
Mass (Da):27,888
Last modified:October 1, 1993 - v1
Checksum:i189829AFD0BD6BD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07952 Unassigned DNA. Translation: AAA16520.1.
L15442 Unassigned DNA. Translation: AAA16840.1.
U33007 Genomic DNA. Translation: AAB64880.1.
BK006938 Genomic DNA. Translation: DAA12252.1.
PIRiS37604.
RefSeqiNP_010698.1. NM_001180718.1.

Genome annotation databases

EnsemblFungiiYDR410C; YDR410C; YDR410C.
GeneIDi852019.
KEGGisce:YDR410C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L07952 Unassigned DNA. Translation: AAA16520.1.
L15442 Unassigned DNA. Translation: AAA16840.1.
U33007 Genomic DNA. Translation: AAB64880.1.
BK006938 Genomic DNA. Translation: DAA12252.1.
PIRiS37604.
RefSeqiNP_010698.1. NM_001180718.1.

3D structure databases

ProteinModelPortaliP32584.
SMRiP32584.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32470. 15 interactors.
DIPiDIP-2678N.
IntActiP32584. 3 interactors.
MINTiMINT-1651790.

Chemistry databases

BindingDBiP32584.
ChEMBLiCHEMBL4385.

Proteomic databases

MaxQBiP32584.
PRIDEiP32584.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR410C; YDR410C; YDR410C.
GeneIDi852019.
KEGGisce:YDR410C.

Organism-specific databases

EuPathDBiFungiDB:YDR410C.
SGDiS000002818. STE14.

Phylogenomic databases

GeneTreeiENSGT00390000017394.
HOGENOMiHOG000213961.
InParanoidiP32584.
KOiK00587.
OMAiLPFIRGY.
OrthoDBiEOG092C5DVA.

Enzyme and pathway databases

BioCyciYEAST:G3O-29953-MONOMER.
ReactomeiR-SCE-163841. Gamma carboxylation, hypusine formation and arylsulfatase activation.

Miscellaneous databases

PROiP32584.

Family and domain databases

InterProiIPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view]
PfamiPF04140. ICMT. 1 hit.
[Graphical view]
PROSITEiPS51564. SAM_ICMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTE14_YEAST
AccessioniPrimary (citable) accession number: P32584
Secondary accession number(s): D6VT42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2690 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.