P32583 (SRP40_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Suppressor protein SRP40

Gene names

Name:	SRP40
Ordered Locus Names:	YKR092C
ORF Names:	YKR412A

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Not known; weak suppressor of a mutant of the subunit AC40 of DNA dependent RNA polymerase I and III.
Miscellaneous	Present with 12900 molecules/cell in log phase SD medium. Ref.5

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	nucleolus Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Suppressor protein SRP40		PRO_0000072192
Regions
	Compositional bias	25 – 314	290	Asp/Ser-rich
Amino acid modifications
	Modified residue	133	1	Phosphoserine Ref.7
	Modified residue	289	1	Phosphothreonine Ref.6 Ref.8
	Modified residue	293	1	Phosphoserine Ref.8
	Modified residue	301	1	Phosphoserine Ref.8
	Modified residue	308	1	Phosphoserine Ref.8
	Modified residue	313	1	Phosphoserine Ref.8
	Modified residue	389	1	Phosphoserine Ref.7
	Modified residue	394	1	Phosphoserine Ref.7 Ref.9
Experimental info
	Sequence conflict	400	1	G → N in AAA35091. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P32583 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: 8EA007695AF4BA1D	FASTA	406	41,015
	10 20 30 40 50 60 MASKKIKVDE VPKLSVKEKE IEEKSSSSSS SSSSSSSSSS SSSSSSSSSG ESSSSSSSSS 70 80 90 100 110 120 SSSSSDSSDS SDSESSSSSS SSSSSSSSSS DSESSSESDS SSSGSSSSSS SSSDESSSES 130 140 150 160 170 180 ESEDETKKRA RESDNEDAKE TKKAKTEPES SSSSESSSSG SSSSSESESG SESDSDSSSS 190 200 210 220 230 240 SSSSSDSESD SESDSQSSSS SSSSDSSSDS DSSSSDSSSD SDSSSSSSSS SSDSDSDSDS 250 260 270 280 290 300 SSDSDSSGSS DSSSSSDSSS DESTSSDSSD SDSDSDSGSS SELETKEATA DESKAEETPA 310 320 330 340 350 360 SSNESTPSAS SSSSANKLNI PAGTDEIKEG QRKHFSRVDR SKINFEAWEL TDNTYKGAAG 370 380 390 400 TWGEKANEKL GRVRGKDFTK NKNKMKRGSY RGGSITLESG SYKFQD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Interactions between three common subunits of yeast RNA polymerases I and III." Lalo D., Carles C., Sentenac A., Thuriaux P. Proc. Natl. Acad. Sci. U.S.A. 90:5524-5528(1993) [PubMed: 8516295] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]	"The complete sequence of a 15,820 bp segment of Saccharomyces cerevisiae chromosome XI contains the UBI2 and MPL1 genes and three new open reading frames." Bou G., Esteban P.F., Baladron V., Gonzalez G.A., Cantalejo J.G., Remacha M.A., Jimenez A., del Rey F., Ballesta J.P.G., Revuelta J.L. Yeast 9:1349-1354(1993) [PubMed: 8154186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-289, MASS SPECTROMETRY. Strain: ADR376.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133; SER-389 AND SER-394, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-289; SER-293; SER-301; SER-308 AND SER-313, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-394, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L11275 Genomic DNA. Translation: AAA35091.1. X73541 Genomic DNA. Translation: CAA51946.1. Z28317 Genomic DNA. Translation: CAA82171.1. BK006944 Genomic DNA. Translation: DAA09242.1.
PIR	S38170.
RefSeq	NP_013018.1. NM_001179882.1.
3D structure databases
ProteinModelPortal	P32583.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2115N.
IntAct	P32583. 9 interactions.
MINT	MINT-472548.
STRING	P32583.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR092C; YKR092C; YKR092C.
GeneID	853967.
KEGG	sce:YKR092C.
NMPDR	fig|4932.3.peg.4005.
Organism-specific databases
CYGD	YKR092c.
SGD	S000001800. SRP40.
Phylogenomic databases
eggNOG	fuNOG10840.
GeneTree	EFGT00050000000009.
OMA	SGKEGEM.
OrthoDB	EOG4M68TB.
Gene expression databases
ArrayExpress	P32583.
Genevestigator	P32583.
GermOnline	YKR092C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007718. SRP40_C. [Graphical view]
Pfam	PF05022. SRP40_C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975404.

Entry information

Entry name

SRP40_YEAST

Accession

Primary (citable) accession number: P32583
Secondary accession number(s): D6VXF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	June 1, 1994
Last modified:	November 16, 2011
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program