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Protein

Cystathionine beta-synthase

Gene

CYS4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-serine + L-homocysteine = L-cystathionine + H2O.

Cofactori

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Cystathionine beta-synthase (CYS4)
  2. Cystathionine gamma-lyase (CYS3)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84Pyridoxal phosphateBy similarity1
Binding sitei289Pyridoxal phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular amino acid biosynthetic process Source: UniProtKB-KW
  • cysteine biosynthetic process from serine Source: SGD
  • cysteine biosynthetic process via cystathionine Source: SGD
  • hydrogen sulfide biosynthetic process Source: SGD
  • transsulfuration Source: SGD
  • traversing start control point of mitotic cell cycle Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:YGR155W-MONOMER.
YEAST:YGR155W-MONOMER.
BRENDAi4.2.1.22. 984.
ReactomeiR-SCE-1614603. Cysteine formation from homocysteine.
SABIO-RKP32582.
UniPathwayiUPA00136; UER00201.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine beta-synthase (EC:4.2.1.22)
Alternative name(s):
Beta-thionase
Serine sulfhydrase
Sulfur transfer protein 4
Gene namesi
Name:CYS4
Synonyms:STR4
Ordered Locus Names:YGR155W
ORF Names:G6667
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR155W.
SGDiS000003387. CYS4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytoplasmic stress granule Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001671351 – 507Cystathionine beta-synthaseAdd BLAST507

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53N6-(pyridoxal phosphate)lysineBy similarity1
Modified residuei134PhosphoserineCombined sources1
Modified residuei350PhosphoserineCombined sources1
Modified residuei424PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32582.
PRIDEiP32582.

PTM databases

iPTMnetiP32582.

Interactioni

Protein-protein interaction databases

BioGridi33403. 142 interactors.
DIPiDIP-1282N.
IntActiP32582. 8 interactors.
MINTiMINT-391362.

Structurei

3D structure databases

ProteinModelPortaliP32582.
SMRiP32582.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini373 – 432CBSPROSITE-ProRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni196 – 200Pyridoxal phosphate bindingBy similarity5

Sequence similaritiesi

Contains 1 CBS domain.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain

Phylogenomic databases

GeneTreeiENSGT00510000047027.
HOGENOMiHOG000217392.
InParanoidiP32582.
KOiK01697.
OMAiEEIWTQT.
OrthoDBiEOG092C2X7L.

Family and domain databases

InterProiIPR000644. CBS_dom.
IPR005857. Cysta_beta_synth.
IPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00291. PALP. 1 hit.
[Graphical view]
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01137. cysta_beta. 1 hit.
PROSITEiPS51371. CBS. 1 hit.
PS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P32582-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKSEQQADS RHNVIDLVGN TPLIALKKLP KALGIKPQIY AKLELYNPGG
60 70 80 90 100
SIKDRIAKSM VEEAEASGRI HPSRSTLIEP TSGNTGIGLA LIGAIKGYRT
110 120 130 140 150
IITLPEKMSN EKVSVLKALG AEIIRTPTAA AWDSPESHIG VAKKLEKEIP
160 170 180 190 200
GAVILDQYNN MMNPEAHYFG TGREIQRQLE DLNLFDNLRA VVAGAGTGGT
210 220 230 240 250
ISGISKYLKE QNDKIQIVGA DPFGSILAQP ENLNKTDITD YKVEGIGYDF
260 270 280 290 300
VPQVLDRKLI DVWYKTDDKP SFKYARQLIS NEGVLVGGSS GSAFTAVVKY
310 320 330 340 350
CEDHPELTED DVIVAIFPDS IRSYLTKFVD DEWLKKNNLW DDDVLARFDS
360 370 380 390 400
SKLEASTTKY ADVFGNATVK DLHLKPVVSV KETAKVTDVI KILKDNGFDQ
410 420 430 440 450
LPVLTEDGKL SGLVTLSELL RKLSINNSNN DNTIKGKYLD FKKLNNFNDV
460 470 480 490 500
SSYNENKSGK KKFIKFDENS KLSDLNRFFE KNSSAVITDG LKPIHIVTKM

DLLSYLA
Length:507
Mass (Da):56,022
Last modified:October 1, 1993 - v1
Checksum:iD0C7059B20FD0746
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2T → A in AAC37401 (PubMed:8022826).Curated1
Sequence conflicti8A → T in AAC37401 (PubMed:8022826).Curated1
Sequence conflicti63Missing in AAC37401 (PubMed:8022826).Curated1
Sequence conflicti104L → W in AAC37401 (PubMed:8022826).Curated1
Sequence conflicti129A → V in AAC37401 (PubMed:8022826).Curated1
Sequence conflicti163N → T in AAC37401 (PubMed:8022826).Curated1
Sequence conflicti407D → Y in AAC37401 (PubMed:8022826).Curated1
Sequence conflicti436 – 437GK → VE in AAC37401 (PubMed:8022826).Curated2
Sequence conflicti441F → V in AAC37401 (PubMed:8022826).Curated1
Sequence conflicti481K → E in AAC37401 (PubMed:8022826).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti504S → N in strain: UCD932. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72922 Genomic DNA. Translation: CAA51426.1.
D16502 Genomic DNA. Translation: BAA03952.1.
L14578 Unassigned DNA. Translation: AAC37401.1.
DQ393806 Genomic DNA. Translation: ABD57960.1.
DQ393807 Genomic DNA. Translation: ABD57961.1.
DQ393808 Genomic DNA. Translation: ABD57962.1.
DQ393809 Genomic DNA. Translation: ABD57963.1.
X85807 Genomic DNA. Translation: CAA59812.1.
Z72940 Genomic DNA. Translation: CAA97169.1.
BK006941 Genomic DNA. Translation: DAA08246.1.
PIRiA48661.
RefSeqiNP_011671.3. NM_001181284.3.

Genome annotation databases

EnsemblFungiiYGR155W; YGR155W; YGR155W.
GeneIDi853059.
KEGGisce:YGR155W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72922 Genomic DNA. Translation: CAA51426.1.
D16502 Genomic DNA. Translation: BAA03952.1.
L14578 Unassigned DNA. Translation: AAC37401.1.
DQ393806 Genomic DNA. Translation: ABD57960.1.
DQ393807 Genomic DNA. Translation: ABD57961.1.
DQ393808 Genomic DNA. Translation: ABD57962.1.
DQ393809 Genomic DNA. Translation: ABD57963.1.
X85807 Genomic DNA. Translation: CAA59812.1.
Z72940 Genomic DNA. Translation: CAA97169.1.
BK006941 Genomic DNA. Translation: DAA08246.1.
PIRiA48661.
RefSeqiNP_011671.3. NM_001181284.3.

3D structure databases

ProteinModelPortaliP32582.
SMRiP32582.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33403. 142 interactors.
DIPiDIP-1282N.
IntActiP32582. 8 interactors.
MINTiMINT-391362.

PTM databases

iPTMnetiP32582.

Proteomic databases

MaxQBiP32582.
PRIDEiP32582.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR155W; YGR155W; YGR155W.
GeneIDi853059.
KEGGisce:YGR155W.

Organism-specific databases

EuPathDBiFungiDB:YGR155W.
SGDiS000003387. CYS4.

Phylogenomic databases

GeneTreeiENSGT00510000047027.
HOGENOMiHOG000217392.
InParanoidiP32582.
KOiK01697.
OMAiEEIWTQT.
OrthoDBiEOG092C2X7L.

Enzyme and pathway databases

UniPathwayiUPA00136; UER00201.
BioCyciMetaCyc:YGR155W-MONOMER.
YEAST:YGR155W-MONOMER.
BRENDAi4.2.1.22. 984.
ReactomeiR-SCE-1614603. Cysteine formation from homocysteine.
SABIO-RKP32582.

Miscellaneous databases

PROiP32582.

Family and domain databases

InterProiIPR000644. CBS_dom.
IPR005857. Cysta_beta_synth.
IPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00291. PALP. 1 hit.
[Graphical view]
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01137. cysta_beta. 1 hit.
PROSITEiPS51371. CBS. 1 hit.
PS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBS_YEAST
AccessioniPrimary (citable) accession number: P32582
Secondary accession number(s): D6VUT5
, Q05177, Q27JK1, Q27JK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 160 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 41900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.