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Protein

Cystathionine beta-synthase

Gene

CYS4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 41900 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

L-serine + L-homocysteine = L-cystathionine + H2O.

Cofactori

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Cystathionine beta-synthase (CYS4)
  2. Cystathionine gamma-lyase (CYS3)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84Pyridoxal phosphateBy similarity1
Binding sitei289Pyridoxal phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cysteine biosynthetic process from serine Source: SGD
  • cysteine biosynthetic process via cystathionine Source: SGD
  • hydrogen sulfide biosynthetic process Source: SGD
  • transsulfuration Source: SGD
  • traversing start control point of mitotic cell cycle Source: SGD

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Cysteine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:YGR155W-MONOMER
YEAST:YGR155W-MONOMER
BRENDAi4.2.1.22 984
ReactomeiR-SCE-1614603 Cysteine formation from homocysteine
SABIO-RKP32582
UniPathwayiUPA00136; UER00201

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine beta-synthase (EC:4.2.1.22)
Alternative name(s):
Beta-thionase
Serine sulfhydrase
Sulfur transfer protein 4
Gene namesi
Name:CYS4
Synonyms:STR4
Ordered Locus Names:YGR155W
ORF Names:G6667
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR155W
SGDiS000003387 CYS4

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001671351 – 507Cystathionine beta-synthaseAdd BLAST507

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53N6-(pyridoxal phosphate)lysineBy similarity1
Modified residuei134PhosphoserineCombined sources1
Modified residuei350PhosphoserineCombined sources1
Modified residuei424PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP32582
PaxDbiP32582
PRIDEiP32582

PTM databases

iPTMnetiP32582

Interactioni

Protein-protein interaction databases

BioGridi33403, 182 interactors
DIPiDIP-1282N
IntActiP32582, 30 interactors
MINTiP32582
STRINGi4932.YGR155W

Structurei

3D structure databases

ProteinModelPortaliP32582
SMRiP32582
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini373 – 432CBSPROSITE-ProRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni196 – 200Pyridoxal phosphate bindingBy similarity5

Sequence similaritiesi

Keywords - Domaini

CBS domain

Phylogenomic databases

GeneTreeiENSGT00510000047027
HOGENOMiHOG000217392
InParanoidiP32582
KOiK01697
OMAiSYLSKFA
OrthoDBiEOG092C2X7L

Family and domain databases

InterProiView protein in InterPro
IPR000644 CBS_dom
IPR005857 Cysta_beta_synth
IPR001216 P-phosphate_BS
IPR001926 PLP-dep
IPR036052 Trypto_synt_PLP_dependent
PfamiView protein in Pfam
PF00571 CBS, 2 hits
PF00291 PALP, 1 hit
SMARTiView protein in SMART
SM00116 CBS, 2 hits
SUPFAMiSSF53686 SSF53686, 1 hit
TIGRFAMsiTIGR01137 cysta_beta, 1 hit
PROSITEiView protein in PROSITE
PS51371 CBS, 1 hit
PS00901 CYS_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

P32582-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKSEQQADS RHNVIDLVGN TPLIALKKLP KALGIKPQIY AKLELYNPGG
60 70 80 90 100
SIKDRIAKSM VEEAEASGRI HPSRSTLIEP TSGNTGIGLA LIGAIKGYRT
110 120 130 140 150
IITLPEKMSN EKVSVLKALG AEIIRTPTAA AWDSPESHIG VAKKLEKEIP
160 170 180 190 200
GAVILDQYNN MMNPEAHYFG TGREIQRQLE DLNLFDNLRA VVAGAGTGGT
210 220 230 240 250
ISGISKYLKE QNDKIQIVGA DPFGSILAQP ENLNKTDITD YKVEGIGYDF
260 270 280 290 300
VPQVLDRKLI DVWYKTDDKP SFKYARQLIS NEGVLVGGSS GSAFTAVVKY
310 320 330 340 350
CEDHPELTED DVIVAIFPDS IRSYLTKFVD DEWLKKNNLW DDDVLARFDS
360 370 380 390 400
SKLEASTTKY ADVFGNATVK DLHLKPVVSV KETAKVTDVI KILKDNGFDQ
410 420 430 440 450
LPVLTEDGKL SGLVTLSELL RKLSINNSNN DNTIKGKYLD FKKLNNFNDV
460 470 480 490 500
SSYNENKSGK KKFIKFDENS KLSDLNRFFE KNSSAVITDG LKPIHIVTKM

DLLSYLA
Length:507
Mass (Da):56,022
Last modified:October 1, 1993 - v1
Checksum:iD0C7059B20FD0746
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2T → A in AAC37401 (PubMed:8022826).Curated1
Sequence conflicti8A → T in AAC37401 (PubMed:8022826).Curated1
Sequence conflicti63Missing in AAC37401 (PubMed:8022826).Curated1
Sequence conflicti104L → W in AAC37401 (PubMed:8022826).Curated1
Sequence conflicti129A → V in AAC37401 (PubMed:8022826).Curated1
Sequence conflicti163N → T in AAC37401 (PubMed:8022826).Curated1
Sequence conflicti407D → Y in AAC37401 (PubMed:8022826).Curated1
Sequence conflicti436 – 437GK → VE in AAC37401 (PubMed:8022826).Curated2
Sequence conflicti441F → V in AAC37401 (PubMed:8022826).Curated1
Sequence conflicti481K → E in AAC37401 (PubMed:8022826).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti504S → N in strain: UCD932. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72922 Genomic DNA Translation: CAA51426.1
D16502 Genomic DNA Translation: BAA03952.1
L14578 Unassigned DNA Translation: AAC37401.1
DQ393806 Genomic DNA Translation: ABD57960.1
DQ393807 Genomic DNA Translation: ABD57961.1
DQ393808 Genomic DNA Translation: ABD57962.1
DQ393809 Genomic DNA Translation: ABD57963.1
X85807 Genomic DNA Translation: CAA59812.1
Z72940 Genomic DNA Translation: CAA97169.1
BK006941 Genomic DNA Translation: DAA08246.1
PIRiA48661
RefSeqiNP_011671.3, NM_001181284.3

Genome annotation databases

EnsemblFungiiYGR155W; YGR155W; YGR155W
GeneIDi853059
KEGGisce:YGR155W

Similar proteinsi

Entry informationi

Entry nameiCBS_YEAST
AccessioniPrimary (citable) accession number: P32582
Secondary accession number(s): D6VUT5
, Q05177, Q27JK1, Q27JK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: May 23, 2018
This is version 173 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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