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Protein

ATP-dependent RNA helicase SUV3, mitochondrial

Gene

SUV3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for intron-independent turnover and processing of mitochondrial RNA. It is a key control element in nuclear-mitochondrial interactions.1 Publication

Miscellaneous

Present with 1440 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi239 – 246ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • Group I intron splicing Source: SGD
  • mitochondrial DNA replication Source: SGD
  • mitochondrial RNA 3'-end processing Source: GO_Central
  • mitochondrial RNA catabolic process Source: SGD
  • RNA phosphodiester bond hydrolysis, exonucleolytic Source: GOC

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33944-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase SUV3, mitochondrial (EC:3.6.4.13)
Gene namesi
Name:SUV3
Ordered Locus Names:YPL029W
ORF Names:LPB2W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL029W.
SGDiS000005950. SUV3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi272V → L in suv3-1; probably excised introns are less efficiently released. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 25MitochondrionSequence analysisAdd BLAST25
ChainiPRO_000001330626 – 737ATP-dependent RNA helicase SUV3, mitochondrialAdd BLAST712

Proteomic databases

MaxQBiP32580.
PRIDEiP32580.

Interactioni

Subunit structurei

MSU1 and SUV3 are the two components of the mitochondrial degradosome (mtEXO).1 Publication

Protein-protein interaction databases

BioGridi36149. 214 interactors.
DIPiDIP-6302N.
IntActiP32580. 3 interactors.
MINTiMINT-698589.
STRINGi4932.YPL029W.

Structurei

3D structure databases

ProteinModelPortaliP32580.
SMRiP32580.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini226 – 365Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST140
Domaini390 – 546Helicase C-terminalPROSITE-ProRule annotationAdd BLAST157

Sequence similaritiesi

Belongs to the helicase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000003100.
HOGENOMiHOG000248220.
InParanoidiP32580.
KOiK17675.
OMAiGTIEMIP.
OrthoDBiEOG092C2ASH.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
InterProiView protein in InterPro
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR022192. SUV3_C.
PfamiView protein in Pfam
PF00271. Helicase_C. 1 hit.
PF12513. SUV3_C. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32580-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALVKYSTVF FPLRSLRLFV SIKKAYYHSE PHSIDLFHDK DWIVKRPKFL
60 70 80 90 100
NLPKNEHSKL DIFQFNFNKS ESNNVYLQDS SFKDNLDKAM QFIYNDKLSS
110 120 130 140 150
LDAKQVPIKN LAWLKLRDYI YQQLKDPKLQ AKTYVPSVSE IIHPSSPGNL
160 170 180 190 200
ISLLINCNKI SNLVWKSVLK YSLSNNITTL DKFIHVLQQT FDHVYEQEIL
210 220 230 240 250
PMMTNTDDTD GAHNVDITNP AEWFPEARKI RRHIIMHIGP TNSGKTYRAL
260 270 280 290 300
QKLKSVDRGY YAGPLRLLAR EVYDRFHAEK IRCNLLTGEE VIRDLDDRGN
310 320 330 340 350
SAGLTSGTVE MVPINQKFDV VVLDEIQMMS DGDRGWAWTN ALLGVVSKEV
360 370 380 390 400
HLCGEKSVLP LVKSIVKMTG DKLTINEYER LGKLSVEEKP IKDGIKGLRK
410 420 430 440 450
GDCVVAFSKK KILDLKLKIE KDTNLKVAVI YGSLPPETRV QQAALFNNGE
460 470 480 490 500
YDIMVASDAI GMGLNLSIDR VVFTTNMKYN GEELMEMTSS QIKQIGGRAG
510 520 530 540 550
RFKSRSASGG VPQGFITSFE SKVLKSVRKA IEAPVEYLKT AVTWPTDEIC
560 570 580 590 600
AQLMTQFPPG TPTSVLLQTI SDELEKSSDN LFTLSDLKSK LKVIGLFEHM
610 620 630 640 650
EDIPFFDKLK LSNAPVKDMP MVTKAFTKFC ETIAKRHTRG LLSYRLPFNL
660 670 680 690 700
LDYNCIPNES YSLEVYESLY NIITLYFWLS NRYPNYFIDM ESAKDLKYFC
710 720 730
EMIIFEKLDR LKKNPYAHKP FGSTRGHLSS SRRRLRT
Length:737
Mass (Da):84,324
Last modified:November 1, 1997 - v2
Checksum:i17D18200E5D18099
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti165W → R in AAA35135 (PubMed:1379722).Curated1
Sequence conflicti664E → D in AAA35135 (PubMed:1379722).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91167 Genomic DNA. Translation: AAA35135.1.
U36624 Genomic DNA. Translation: AAB68158.1.
BK006949 Genomic DNA. Translation: DAA11400.1.
PIRiS63453.
RefSeqiNP_015296.1. NM_001183843.1.

Genome annotation databases

EnsemblFungiiYPL029W; YPL029W; YPL029W.
GeneIDi856078.
KEGGisce:YPL029W.

Similar proteinsi

Entry informationi

Entry nameiSUV3_YEAST
AccessioniPrimary (citable) accession number: P32580
Secondary accession number(s): D6W3Y4, Q02649
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: November 1, 1997
Last modified: November 22, 2017
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names