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Reviewed, UniProtKB/Swiss-Prot P32577 (CSK_RAT)

Last modified June 16, 2009. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Tyrosine-protein kinase CSK
    EC=2.7.10.2
Alternative name(s):
    C-SRC kinase
Gene names
Name: Csk
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length450 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure

Interacts with phosphorylated SIT1, LIME1 and TGFB1I1. Interacts with PTPN8 By similarity. Interacts with phosphorylated PAG1.

Subcellular location

Cytoplasm By similarity. Cell membrane By similarity. Note: Mainly cytoplasmic, also present in lipid rafts By similarity.

Tissue specificity

Enriched in lymphoid tissues.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.

Contains 1 protein kinase domain.

Contains 1 SH2 domain.

Contains 1 SH3 domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 450450Tyrosine-protein kinase CSK
PRO_0000088072

Regions

Domain9 – 7062SH3
Domain82 – 17190SH2
Domain195 – 445251Protein kinase
Nucleotide binding201 – 2099ATP By similarity
Region9 – 7062Interaction with PTPN8 By similarity

Sites

Active site3141Proton acceptor By similarity
Binding site2221ATP By similarity

Amino acid modifications

Modified residue1841Phosphotyrosine By similarity
Modified residue4161Phosphotyrosine; by autocatalysis By similarity

Secondary structure

1 ........................................................................... 450
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P32577-1 [UniParc].

Last modified October 1, 1993. Version 1.
Checksum: 393DC8D737DAC67A

FASTA45050,746
        10         20         30         40         50         60 
MSAIQASWPS GTECIAKYNF HGTAEQDLPF CKGDVLTIVA VTKDPNWYKA KNKVGREGII 

        70         80         90        100        110        120 
PANYVQKREG VKAGTKLSLM PWFHGKITRE QAERLLYPPE TGLFLVREST NYPGDYTLCV 

       130        140        150        160        170        180 
SCEGKVEHYR IMYHASKLSI DEEVYFENLM QLVEHYTTDA DGLCTRLIKP KVMEGTVAAQ 

       190        200        210        220        230        240 
DEFYRSGWAL NMKELKLLQT IGKGEFGDVM LGDYRGNKVA VKCIKNDATA QAFLAEASVM 

       250        260        270        280        290        300 
TQLRHSNLVQ LLGVIVEEKG GLYIVTEYMA KGSLVDYLRS RGRSVLGGDC LLKFSLDVCE 

       310        320        330        340        350        360 
AMEYLEGNNF VHRDLAARNV LVSEDNVAKV SDFGLTKEAS STQDTGKLPV KWTAPEALRE 

       370        380        390        400        410        420 
KKFSTKSDVW SFGILLWEIY SFGRVPYPRI PLKDVVPRVE KGYKMDAPDG CPPAVYDVMK 

       430        440        450 
NCWHLDAATR PTFLQLREQL EHIRTHELHL

« Hide

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References

« Hide 'large scale' references
[1]"Cloning of a complementary DNA for a protein-tyrosine kinase that specifically phosphorylates a negative regulatory site of p60c-src."
Nada S., Okada M., McAuley A., Cooper J.A., Nakagawa H.
Nature 351:69-72(1991) [PubMed: 1709258] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Thymus.
[3]"CSK: a protein-tyrosine kinase involved in regulation of src family kinases."
Okada M., Nada S., Yamanashi Y., Yamamoto T., Nakagawa H.
J. Biol. Chem. 266:24249-24252(1991) [PubMed: 1722201] [Abstract]
Cited for: CHARACTERIZATION.
[4]"Transmembrane phosphoprotein Cbp regulates the activities of Src-family tyrosine kinases."
Kawabuchi M., Satomi Y., Takao T., Shimonishi Y., Nada S., Nagai K., Tarakhovsky A., Okada M.
Nature 404:999-1003(2000) [PubMed: 10801129] [Abstract]
Cited for: INTERACTION WITH PAG1.
[5]"Mechanism of Csk-mediated down-regulation of Src family tyrosine kinases in epidermal growth factor signaling."
Matsuoka H., Nada S., Okada M.
J. Biol. Chem. 279:5975-5983(2004) [PubMed: 14613929] [Abstract]
Cited for: INTERACTION WITH PAG1.
+Additional computationally mapped references.

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Cross-references

Sequence databases

X58631 mRNA. Translation: CAA41484.1.
BC098863 mRNA. Translation: AAH98863.1.
IPIIPI00188950.
PIRS15094.
RefSeqNP_001025210.1.
UniGeneRn.2759

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1K9AX-ray2.50A/B/C/D/E/F1-450[»]
ModBaseSearch...

PTM databases

PhosphoSiteP32577.

Genome annotation databases

EnsemblENSRNOG00000019374. Rattus norvegicus. [Contig view]
GeneID315707.
KEGGrno:315707.

Organism-specific databases

RGD1308800. Csk.

Phylogenomic databases

HOVERGENP32577.
OMAP32577. QDLPFCK.

Enzyme and pathway databases

BRENDA2.7.10.2. 248.

Gene expression databases

ArrayExpressP32577.
GermOnlineENSRNOG00000019374. Rattus norvegicus.

Family and domain databases

InterProIPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR001245. Tyr_pkinase.
IPR008266. Tyr_pkinase_AS.
IPR015778. Tyrosine_Protein_Kinase_Csk.
[Graphical view]
Gene3DG3DSA:3.30.505.10. SH2. 1 hit.
PANTHERPTHR23256:SF264. Csk_ProtTyrKin. 1 hit.
PfamPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSPR00401. SH2DOMAIN.
PR00109. TYRKINASE.
ProDomPD000001. Prot_kinase. 1 hit.
PD000093. SH2. 1 hit.
PD000066. SH3. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio669700.

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Entry information

Entry nameCSK_RAT
AccessionPrimary (citable) accession number: P32577
Secondary accession number(s): Q4G003
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: October 1, 1993
Last modified: June 16, 2009
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents