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P32573 (SPS19_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peroxisomal 2,4-dienoyl-CoA reductase SPS19

EC=1.3.1.34
Alternative name(s):
Sporulation-specific protein SPX19
Gene names
Name:SPS19
Synonyms:SPX19
Ordered Locus Names:YNL202W
ORF Names:N1362
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length292 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Auxiliary enzyme of beta-oxidation. Participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA. Dispensable for growth and sporulation on solid acetate and oleate media, but is essential for these processes to occur on petroselineate.

Catalytic activity

Trans-2,3-didehydroacyl-CoA + NADP+ = trans,trans-2,3,4,5-tetradehydroacyl-CoA + NADPH. Ref.6

Subunit structure

Homodimer. Ref.6

Subcellular location

Peroxisome Ref.6.

Developmental stage

Sequentially activated during the process of meiosis and spore formation.

Induction

By oleate. Transcription is regulated by the transcription factors PIP2, OAF1 and ADR1. Weakly induced during sporulation in diploid cells. Ref.6 Ref.7 Ref.8

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Sequence caution

The sequence AAA62403.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAT93138.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA55506.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA96103.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processSporulation
   Cellular componentPeroxisome
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processascospore formation

Inferred from mutant phenotype Ref.5. Source: SGD

fatty acid catabolic process

Inferred from direct assay Ref.6. Source: SGD

   Cellular_componentperoxisomal matrix

Inferred from direct assay Ref.6. Source: SGD

   Molecular_function2,4-dienoyl-CoA reductase (NADPH) activity

Inferred from direct assay Ref.6. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 292291Peroxisomal 2,4-dienoyl-CoA reductase SPS19
PRO_0000054565

Regions

Nucleotide binding29 – 5325NADP By similarity
Motif290 – 2923Microbody targeting signal Potential

Sequences

Sequence LengthMass (Da)Tools
P32573 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: C82D520B1F5969A8

FASTA29231,109
        10         20         30         40         50         60 
MNTANTLDGK FVTEGSWRPD LFKGKVAFVT GGAGTICRVQ TEALVLLGCK AAIVGRDQER 

        70         80         90        100        110        120 
TEQAAKGISQ LAKDKDAVLA IANVDVRNFE QVENAVKKTV EKFGKIDFVI AGAAGNFVCD 

       130        140        150        160        170        180 
FANLSPNAFK SVVDIDLLGS FNTAKACLKE LKKSKGSILF VSATFHYYGV PFQGHVGAAK 

       190        200        210        220        230        240 
AGIDALAKNL AVELGPLGIR SNCIAPGAID NTEGLKRLAG KKYKEKALAK IPLQRLGSTR 

       250        260        270        280        290 
DIAESTVYIF SPAASYVTGT VLVVDGGMWH LGTYFGHELY PEALIKSMTS KL 

« Hide

References

« Hide 'large scale' references
[1]"A 21.7 kb DNA segment on the left arm of yeast chromosome XIV carries WHI3, GCR2, SPX18, SPX19, an homologue to the heat shock gene SSB1 and 8 new open reading frames of unknown function."
Jonniaux J.-L., Coster F., Purnelle B., Goffeau A.
Yeast 10:1639-1645(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Identification of a sporulation-specific promoter regulating divergent transcription of two novel sporulation genes in Saccharomyces cerevisiae."
Coe J.G., Murray L.E., Dawes I.W.
Mol. Gen. Genet. 244:661-672(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-260.
Strain: ATCC 28684 / S14.
[6]"The Saccharomyces cerevisiae peroxisomal 2,4-dienoyl-CoA reductase is encoded by the oleate-inducible gene SPS19."
Gurvitz A., Rottensteiner H., Kilpelaeinen S.H., Hartig A., Hiltunen J.K., Binder M., Dawes I.W., Hamilton B.
J. Biol. Chem. 272:22140-22147(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-13, ENZYME ACTIVITY, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION.
[7]"Regulation of the yeast SPS19 gene encoding peroxisomal 2,4-dienoyl-CoA reductase by the transcription factors Pip2p and Oaf1p: beta-oxidation is dispensable for Saccharomyces cerevisiae sporulation in acetate medium."
Gurvitz A., Rottensteiner H., Hiltunen J.K., Binder M., Dawes I.W., Ruis H., Hamilton B.
Mol. Microbiol. 26:675-685(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[8]"Adr1p-dependent regulation of the oleic acid-inducible yeast gene SPS19 encoding the peroxisomal beta-oxidation auxiliary enzyme 2,4-dienoyl-CoA reductase."
Gurvitz A., Wabnegger L., Rottensteiner H., Dawes I.W., Hartig A., Ruis H., Hamilton B.
Mol. Cell Biol. Res. Commun. 4:81-89(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[9]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X78898 Genomic DNA. Translation: CAA55506.1. Different initiation.
Z71479 Genomic DNA. Translation: CAA96103.1. Different initiation.
AY693119 Genomic DNA. Translation: AAT93138.1. Different initiation.
M90351 Genomic DNA. Translation: AAA62403.1. Different initiation.
BK006947 Genomic DNA. Translation: DAA10352.1.
PIRS50729.
RefSeqNP_014197.2. NM_001183040.1.

3D structure databases

ProteinModelPortalP32573.
SMRP32573. Positions 19-269.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35632. 6 interactions.
DIPDIP-4294N.
MINTMINT-516231.
STRING4932.YNL202W.

Proteomic databases

MaxQBP32573.
PaxDbP32573.
PeptideAtlasP32573.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNL202W; YNL202W; YNL202W.
GeneID855518.
KEGGsce:YNL202W.

Organism-specific databases

SGDS000005146. SPS19.

Phylogenomic databases

eggNOGCOG1028.
GeneTreeENSGT00740000115347.
KOK13237.
OMAGRSHAIT.
OrthoDBEOG7CZKH4.

Enzyme and pathway databases

BioCycMetaCyc:YNL202W-MONOMER.
YEAST:YNL202W-MONOMER.

Gene expression databases

GenevestigatorP32573.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PRINTSPR00081. GDHRDH.
ProtoNetSearch...

Other

NextBio979547.
PROP32573.

Entry information

Entry nameSPS19_YEAST
AccessionPrimary (citable) accession number: P32573
Secondary accession number(s): D6W0Y6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 115 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families