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P32573

- SPS19_YEAST

UniProt

P32573 - SPS19_YEAST

Protein

Peroxisomal 2,4-dienoyl-CoA reductase SPS19

Gene

SPS19

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Auxiliary enzyme of beta-oxidation. Participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA. Dispensable for growth and sporulation on solid acetate and oleate media, but is essential for these processes to occur on petroselineate.

    Catalytic activityi

    Trans-2,3-didehydroacyl-CoA + NADP+ = trans,trans-2,3,4,5-tetradehydroacyl-CoA + NADPH.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi29 – 5325NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 2,4-dienoyl-CoA reductase (NADPH) activity Source: SGD

    GO - Biological processi

    1. ascospore formation Source: SGD
    2. fatty acid catabolic process Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Sporulation

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:YNL202W-MONOMER.
    YEAST:YNL202W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxisomal 2,4-dienoyl-CoA reductase SPS19 (EC:1.3.1.34)
    Alternative name(s):
    Sporulation-specific protein SPX19
    Gene namesi
    Name:SPS19
    Synonyms:SPX19
    Ordered Locus Names:YNL202W
    ORF Names:N1362
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    SGDiS000005146. SPS19.

    Subcellular locationi

    Peroxisome 1 Publication

    GO - Cellular componenti

    1. peroxisomal matrix Source: SGD

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 292291Peroxisomal 2,4-dienoyl-CoA reductase SPS19PRO_0000054565Add
    BLAST

    Proteomic databases

    MaxQBiP32573.
    PaxDbiP32573.
    PeptideAtlasiP32573.

    Expressioni

    Developmental stagei

    Sequentially activated during the process of meiosis and spore formation.

    Inductioni

    By oleate. Transcription is regulated by the transcription factors PIP2, OAF1 and ADR1. Weakly induced during sporulation in diploid cells.3 Publications

    Gene expression databases

    GenevestigatoriP32573.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi35632. 6 interactions.
    DIPiDIP-4294N.
    MINTiMINT-516231.
    STRINGi4932.YNL202W.

    Structurei

    3D structure databases

    ProteinModelPortaliP32573.
    SMRiP32573. Positions 19-269.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi290 – 2923Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1028.
    GeneTreeiENSGT00740000115347.
    KOiK13237.
    OMAiGRSHAIT.
    OrthoDBiEOG7CZKH4.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P32573-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTANTLDGK FVTEGSWRPD LFKGKVAFVT GGAGTICRVQ TEALVLLGCK    50
    AAIVGRDQER TEQAAKGISQ LAKDKDAVLA IANVDVRNFE QVENAVKKTV 100
    EKFGKIDFVI AGAAGNFVCD FANLSPNAFK SVVDIDLLGS FNTAKACLKE 150
    LKKSKGSILF VSATFHYYGV PFQGHVGAAK AGIDALAKNL AVELGPLGIR 200
    SNCIAPGAID NTEGLKRLAG KKYKEKALAK IPLQRLGSTR DIAESTVYIF 250
    SPAASYVTGT VLVVDGGMWH LGTYFGHELY PEALIKSMTS KL 292
    Length:292
    Mass (Da):31,109
    Last modified:January 23, 2007 - v4
    Checksum:iC82D520B1F5969A8
    GO

    Sequence cautioni

    The sequence AAA62403.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAT93138.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA55506.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA96103.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78898 Genomic DNA. Translation: CAA55506.1. Different initiation.
    Z71479 Genomic DNA. Translation: CAA96103.1. Different initiation.
    AY693119 Genomic DNA. Translation: AAT93138.1. Different initiation.
    M90351 Genomic DNA. Translation: AAA62403.1. Different initiation.
    BK006947 Genomic DNA. Translation: DAA10352.1.
    PIRiS50729.
    RefSeqiNP_014197.2. NM_001183040.1.

    Genome annotation databases

    EnsemblFungiiYNL202W; YNL202W; YNL202W.
    GeneIDi855518.
    KEGGisce:YNL202W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78898 Genomic DNA. Translation: CAA55506.1 . Different initiation.
    Z71479 Genomic DNA. Translation: CAA96103.1 . Different initiation.
    AY693119 Genomic DNA. Translation: AAT93138.1 . Different initiation.
    M90351 Genomic DNA. Translation: AAA62403.1 . Different initiation.
    BK006947 Genomic DNA. Translation: DAA10352.1 .
    PIRi S50729.
    RefSeqi NP_014197.2. NM_001183040.1.

    3D structure databases

    ProteinModelPortali P32573.
    SMRi P32573. Positions 19-269.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35632. 6 interactions.
    DIPi DIP-4294N.
    MINTi MINT-516231.
    STRINGi 4932.YNL202W.

    Proteomic databases

    MaxQBi P32573.
    PaxDbi P32573.
    PeptideAtlasi P32573.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL202W ; YNL202W ; YNL202W .
    GeneIDi 855518.
    KEGGi sce:YNL202W.

    Organism-specific databases

    SGDi S000005146. SPS19.

    Phylogenomic databases

    eggNOGi COG1028.
    GeneTreei ENSGT00740000115347.
    KOi K13237.
    OMAi GRSHAIT.
    OrthoDBi EOG7CZKH4.

    Enzyme and pathway databases

    BioCyci MetaCyc:YNL202W-MONOMER.
    YEAST:YNL202W-MONOMER.

    Miscellaneous databases

    NextBioi 979547.
    PROi P32573.

    Gene expression databases

    Genevestigatori P32573.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    ProtoNeti Search...

    Publicationsi

    1. "A 21.7 kb DNA segment on the left arm of yeast chromosome XIV carries WHI3, GCR2, SPX18, SPX19, an homologue to the heat shock gene SSB1 and 8 new open reading frames of unknown function."
      Jonniaux J.-L., Coster F., Purnelle B., Goffeau A.
      Yeast 10:1639-1645(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Identification of a sporulation-specific promoter regulating divergent transcription of two novel sporulation genes in Saccharomyces cerevisiae."
      Coe J.G., Murray L.E., Dawes I.W.
      Mol. Gen. Genet. 244:661-672(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-260.
      Strain: ATCC 28684 / S14.
    6. "The Saccharomyces cerevisiae peroxisomal 2,4-dienoyl-CoA reductase is encoded by the oleate-inducible gene SPS19."
      Gurvitz A., Rottensteiner H., Kilpelaeinen S.H., Hartig A., Hiltunen J.K., Binder M., Dawes I.W., Hamilton B.
      J. Biol. Chem. 272:22140-22147(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-13, ENZYME ACTIVITY, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION.
    7. "Regulation of the yeast SPS19 gene encoding peroxisomal 2,4-dienoyl-CoA reductase by the transcription factors Pip2p and Oaf1p: beta-oxidation is dispensable for Saccharomyces cerevisiae sporulation in acetate medium."
      Gurvitz A., Rottensteiner H., Hiltunen J.K., Binder M., Dawes I.W., Ruis H., Hamilton B.
      Mol. Microbiol. 26:675-685(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    8. "Adr1p-dependent regulation of the oleic acid-inducible yeast gene SPS19 encoding the peroxisomal beta-oxidation auxiliary enzyme 2,4-dienoyl-CoA reductase."
      Gurvitz A., Wabnegger L., Rottensteiner H., Dawes I.W., Hartig A., Ruis H., Hamilton B.
      Mol. Cell Biol. Res. Commun. 4:81-89(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSPS19_YEAST
    AccessioniPrimary (citable) accession number: P32573
    Secondary accession number(s): D6W0Y6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1993
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 116 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3