Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Peroxisomal 2,4-dienoyl-CoA reductase SPS19

Gene

SPS19

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Auxiliary enzyme of beta-oxidation. Participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA. Dispensable for growth and sporulation on solid acetate and oleate media, but is essential for these processes to occur on petroselineate.

Catalytic activityi

Trans-2,3-didehydroacyl-CoA + NADP+ = trans,trans-2,3,4,5-tetradehydroacyl-CoA + NADPH.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 53NADPBy similarityAdd BLAST25

GO - Molecular functioni

  • 2,4-dienoyl-CoA reductase (NADPH) activity Source: SGD

GO - Biological processi

  • ascospore formation Source: SGD
  • fatty acid catabolic process Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processSporulation
LigandNADP

Enzyme and pathway databases

BioCyciMetaCyc:YNL202W-MONOMER.
YEAST:YNL202W-MONOMER.
ReactomeiR-SCE-193144. Estrogen biosynthesis.
R-SCE-2142700. Synthesis of Lipoxins (LX).
R-SCE-389599. Alpha-oxidation of phytanate.
R-SCE-390247. Beta-oxidation of very long chain fatty acids.
R-SCE-5365859. RA biosynthesis pathway.
R-SCE-5661270. Catabolism of glucuronate to xylulose-5-phosphate.
R-SCE-70895. Branched-chain amino acid catabolism.
R-SCE-75105. Fatty acyl-CoA biosynthesis.
R-SCE-77111. Synthesis of Ketone Bodies.
R-SCE-77288. mitochondrial fatty acid beta-oxidation of unsaturated fatty acids.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal 2,4-dienoyl-CoA reductase SPS19 (EC:1.3.1.34)
Alternative name(s):
Sporulation-specific protein SPX19
Gene namesi
Name:SPS19
Synonyms:SPX19
Ordered Locus Names:YNL202W
ORF Names:N1362
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL202W.
SGDiS000005146. SPS19.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000545652 – 292Peroxisomal 2,4-dienoyl-CoA reductase SPS19Add BLAST291

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki188Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP32573.
PRIDEiP32573.

Expressioni

Developmental stagei

Sequentially activated during the process of meiosis and spore formation.

Inductioni

By oleate. Transcription is regulated by the transcription factors PIP2, OAF1 and ADR1. Weakly induced during sporulation in diploid cells.3 Publications

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi35632. 48 interactors.
DIPiDIP-4294N.
IntActiP32573. 3 interactors.
MINTiMINT-516231.
STRINGi4932.YNL202W.

Structurei

3D structure databases

ProteinModelPortaliP32573.
SMRiP32573.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi290 – 292Microbody targeting signalSequence analysis3

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00760000118868.
InParanoidiP32573.
KOiK13237.
OMAiNKTEMAH.
OrthoDBiEOG092C3TSH.

Family and domain databases

InterProiView protein in InterPro
IPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P32573-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTANTLDGK FVTEGSWRPD LFKGKVAFVT GGAGTICRVQ TEALVLLGCK
60 70 80 90 100
AAIVGRDQER TEQAAKGISQ LAKDKDAVLA IANVDVRNFE QVENAVKKTV
110 120 130 140 150
EKFGKIDFVI AGAAGNFVCD FANLSPNAFK SVVDIDLLGS FNTAKACLKE
160 170 180 190 200
LKKSKGSILF VSATFHYYGV PFQGHVGAAK AGIDALAKNL AVELGPLGIR
210 220 230 240 250
SNCIAPGAID NTEGLKRLAG KKYKEKALAK IPLQRLGSTR DIAESTVYIF
260 270 280 290
SPAASYVTGT VLVVDGGMWH LGTYFGHELY PEALIKSMTS KL
Length:292
Mass (Da):31,109
Last modified:January 23, 2007 - v4
Checksum:iC82D520B1F5969A8
GO

Sequence cautioni

The sequence AAA62403 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT93138 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA55506 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA96103 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78898 Genomic DNA. Translation: CAA55506.1. Different initiation.
Z71479 Genomic DNA. Translation: CAA96103.1. Different initiation.
AY693119 Genomic DNA. Translation: AAT93138.1. Different initiation.
M90351 Genomic DNA. Translation: AAA62403.1. Different initiation.
BK006947 Genomic DNA. Translation: DAA10352.1.
PIRiS50729.
RefSeqiNP_014197.2. NM_001183040.1.

Genome annotation databases

EnsemblFungiiYNL202W; YNL202W; YNL202W.
GeneIDi855518.
KEGGisce:YNL202W.

Similar proteinsi

Entry informationi

Entry nameiSPS19_YEAST
AccessioniPrimary (citable) accession number: P32573
Secondary accession number(s): D6W0Y6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1993
Last sequence update: January 23, 2007
Last modified: September 27, 2017
This is version 139 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names