P32573 (SPS19_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxisomal 2,4-dienoyl-CoA reductase SPS19
EC=1.3.1.34

Alternative name(s):
Sporulation-specific protein SPX19

Gene names

Name:	SPS19
Synonyms:	SPX19
Ordered Locus Names:	YNL202W
ORF Names:	N1362

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Auxiliary enzyme of beta-oxidation. Participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA. Dispensable for growth and sporulation on solid acetate and oleate media, but is essential for these processes to occur on petroselineate.
Catalytic activity	Trans-2,3-didehydroacyl-CoA + NADP+ = trans,trans-2,3,4,5-tetradehydroacyl-CoA + NADPH. Ref.6
Subunit structure	Homodimer. Ref.6
Subcellular location	Peroxisome Ref.6.
Developmental stage	Sequentially activated during the process of meiosis and spore formation.
Induction	By oleate. Transcription is regulated by the transcription factors PIP2, OAF1 and ADR1. Weakly induced during sporulation in diploid cells. Ref.6 Ref.7 Ref.8
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Sequence caution	The sequence AAA62403.1 differs from that shown. Reason: Erroneous initiation. The sequence AAT93138.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA55506.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA96103.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Sporulation
Cellular component	Peroxisome
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ascospore formation Inferred from expression pattern Ref.5. Source: SGD fatty acid catabolic process Inferred from direct assay Ref.6. Source: SGD
Cellular_component	peroxisomal matrix Inferred from direct assay Ref.6. Source: SGD
Molecular_function	2,4-dienoyl-CoA reductase (NADPH) activity Inferred from direct assay Ref.6. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 292	291	Peroxisomal 2,4-dienoyl-CoA reductase SPS19		PRO_0000054565
Regions
	Nucleotide binding	29 – 53	25	NADP By similarity
	Motif	290 – 292	3	Microbody targeting signal Potential

Sequences

	Sequence		Length	Mass (Da)
	P32573 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: C82D520B1F5969A8	FASTA	292	31,109
	10 20 30 40 50 60 MNTANTLDGK FVTEGSWRPD LFKGKVAFVT GGAGTICRVQ TEALVLLGCK AAIVGRDQER 70 80 90 100 110 120 TEQAAKGISQ LAKDKDAVLA IANVDVRNFE QVENAVKKTV EKFGKIDFVI AGAAGNFVCD 130 140 150 160 170 180 FANLSPNAFK SVVDIDLLGS FNTAKACLKE LKKSKGSILF VSATFHYYGV PFQGHVGAAK 190 200 210 220 230 240 AGIDALAKNL AVELGPLGIR SNCIAPGAID NTEGLKRLAG KKYKEKALAK IPLQRLGSTR 250 260 270 280 290 DIAESTVYIF SPAASYVTGT VLVVDGGMWH LGTYFGHELY PEALIKSMTS KL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 21.7 kb DNA segment on the left arm of yeast chromosome XIV carries WHI3, GCR2, SPX18, SPX19, an homologue to the heat shock gene SSB1 and 8 new open reading frames of unknown function." Jonniaux J.-L., Coster F., Purnelle B., Goffeau A. Yeast 10:1639-1645(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Identification of a sporulation-specific promoter regulating divergent transcription of two novel sporulation genes in Saccharomyces cerevisiae." Coe J.G., Murray L.E., Dawes I.W. Mol. Gen. Genet. 244:661-672(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-260. Strain: ATCC 28684 / S14.
[6]	"The Saccharomyces cerevisiae peroxisomal 2,4-dienoyl-CoA reductase is encoded by the oleate-inducible gene SPS19." Gurvitz A., Rottensteiner H., Kilpelaeinen S.H., Hartig A., Hiltunen J.K., Binder M., Dawes I.W., Hamilton B. J. Biol. Chem. 272:22140-22147(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-13, ENZYME ACTIVITY, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION.
[7]	"Regulation of the yeast SPS19 gene encoding peroxisomal 2,4-dienoyl-CoA reductase by the transcription factors Pip2p and Oaf1p: beta-oxidation is dispensable for Saccharomyces cerevisiae sporulation in acetate medium." Gurvitz A., Rottensteiner H., Hiltunen J.K., Binder M., Dawes I.W., Ruis H., Hamilton B. Mol. Microbiol. 26:675-685(1997) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[8]	"Adr1p-dependent regulation of the oleic acid-inducible yeast gene SPS19 encoding the peroxisomal beta-oxidation auxiliary enzyme 2,4-dienoyl-CoA reductase." Gurvitz A., Wabnegger L., Rottensteiner H., Dawes I.W., Hartig A., Ruis H., Hamilton B. Mol. Cell Biol. Res. Commun. 4:81-89(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78898 Genomic DNA. Translation: CAA55506.1. Different initiation. Z71479 Genomic DNA. Translation: CAA96103.1. Different initiation. AY693119 Genomic DNA. Translation: AAT93138.1. Different initiation. M90351 Genomic DNA. Translation: AAA62403.1. Different initiation. BK006947 Genomic DNA. Translation: DAA10352.1.
PIR	S50729.
RefSeq	NP_014197.2. NM_001183040.1.
3D structure databases
ProteinModelPortal	P32573.
SMR	P32573. Positions 19-269.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4294N.
MINT	MINT-516231.
STRING	4932.YNL202W.
Proteomic databases
PaxDb	P32573.
PeptideAtlas	P32573.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNL202W; YNL202W; YNL202W.
GeneID	855518.
KEGG	sce:YNL202W.
Organism-specific databases
SGD	S000005146. SPS19.
Phylogenomic databases
eggNOG	COG1028.
GeneTree	ENSGT00690000101945.
KO	K13237.
OMA	SATFHYY.
OrthoDB	EOG4VQDZH.
Gene expression databases
Genevestigator	P32573.
GermOnline	YNL202W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
PRINTS	PR00081. GDHRDH.
PROSITE	PS00061. ADH_SHORT. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	979547.

Entry information

Entry name

SPS19_YEAST

Accession

Primary (citable) accession number: P32573
Secondary accession number(s): D6W0Y6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1993
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 107 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program